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The map label for this gene is tig

Identifier: 209694397

GI number: 209694397

Start: 907133

End: 908431

Strand: Direct

Name: tig

Synonym: VSAL_I0817

Alternate gene names: 209694397

Gene position: 907133-908431 (Clockwise)

Preceding gene: 209694396

Following gene: 209694398

Centisome position: 27.28

GC content: 41.26

Gene sequence:

>1299_bases
ATGCAAGTTACCGTTGAAACTAAAGAAGGCCTAGAGCGCGTTCTAACTATTACTGTTCCTGCTGCAAACATTGAAGATGC
TGTAAATGCAGAGCTTCGTAACATCGCTAAAAATCGTCGTTTTGATGGCTTCCGTAAAGGTAAAGTTCCAATGAAGATGG
TTGCTAAAATGTACGGCAGTGCTGTTCGTCACGATAAAATGGGTGAAGTTATGCAACGTCACTTCATTGAAGCGATCGTT
AAAGACAAAATCAACCCAGCAGGCGCTCCAACATTTGCACCAGTTGAATTTGCTGAAGGCCAAGATTTAGTATTCACTGC
AACATTTGAAATCTACCCAGAAGTAACACTTCAAGGTCTAGACAAAGTTGTTGTTGAGAAACCACAAGTTGAAGTTAAAG
ACGAAGACGTAGCAGAAATGCTAGAAACGTTACGTAAGCAACAAGCTACTTGGTCAGATGCTGACGTTGCGGCTGAAGAT
GGCACTCGCGCTACAATCAACTTTGTTGGTTCTATCGATGGTGAGCTATTTGAAGGCGGAAAAGCAGATAACTTCCCTCT
AGAAATGGGCCAAGGTCGTATGATCCCTGGTTTCGAAGACGGTATCAAAGGTAAGAAAGCAGGCGACGAACTAACTATCA
ATGTTAACTTCCCTGAAGATTACCACGCTGAAAACCTAAAAGGTAAAGCTGCAGAATTTGCTATTACAGTAGTTAAAGTT
GAAGCTCGTGAACTTCCAGAAATGAACGATGAGTTCGTAACTAAGTTTGGTGCTCAAGGCGGCGTTGAAGGTCTTAAAAC
TGAAGTTCGTAAGAACATGGAGCGTGAATTAGCTCAAACAGTTAAAAACAAGATCAAAGAACAAGCAATCAATGGCCTAG
TTGAACAAAACAACATTGACGTACCTTCTGCTCTTATCGATCAAGAAGTTCAAGTTTTACGTCAACAAGCAGTTCAACGT
TTTGGCGGTAACGCGGATTCAGCTCCTGAGCTTCCACGTGAATTGTTCGAAGAACAAGCTAAGCGTCGTGTAGTTGTAGG
TCTTCTTCTTGGTGAAGTAATCAAAACTGAAGAACTAAAAGCTGACGATGAGAAAGTAAAAGCACTTATCAACGAAATGG
CTTCTGCTTACGAAGATCCAACTGAAGTTGTTGCTTACTACGAAGGCAACGAACAAATGATGAACAACATGCGTAATGTT
GCTCTAGAAGAACAAGCGATTGATGCTATTTTAGCTAAAGCGCAAGTTTCTGAAAAAGCGGTTGGCTTTAACGAACTAAT
GAATCAACAACCAGCTTAA

Upstream 100 bases:

>100_bases
TTACGGTTTCTATTTTATTAGGCCTGTAACGCAAGGATGCGACACGTATTTTTTGTCGCAAACTCAGAGAACCTGAGTCG
ATTTTGAGGTTTATATAAAC

Downstream 100 bases:

>100_bases
TTTAAAATTAATTAAGTAGTTGACTTAGTTTCAAAAGATCTGCTAATAATGGTTCGAATGAAGCGTCATTCGGACCATTT
ATTTTATAAGGGATACGGTT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MQVTVETKEGLERVLTITVPAANIEDAVNAELRNIAKNRRFDGFRKGKVPMKMVAKMYGSAVRHDKMGEVMQRHFIEAIV
KDKINPAGAPTFAPVEFAEGQDLVFTATFEIYPEVTLQGLDKVVVEKPQVEVKDEDVAEMLETLRKQQATWSDADVAAED
GTRATINFVGSIDGELFEGGKADNFPLEMGQGRMIPGFEDGIKGKKAGDELTINVNFPEDYHAENLKGKAAEFAITVVKV
EARELPEMNDEFVTKFGAQGGVEGLKTEVRKNMERELAQTVKNKIKEQAINGLVEQNNIDVPSALIDQEVQVLRQQAVQR
FGGNADSAPELPRELFEEQAKRRVVVGLLLGEVIKTEELKADDEKVKALINEMASAYEDPTEVVAYYEGNEQMMNNMRNV
ALEEQAIDAILAKAQVSEKAVGFNELMNQQPA

Sequences:

>Translated_432_residues
MQVTVETKEGLERVLTITVPAANIEDAVNAELRNIAKNRRFDGFRKGKVPMKMVAKMYGSAVRHDKMGEVMQRHFIEAIV
KDKINPAGAPTFAPVEFAEGQDLVFTATFEIYPEVTLQGLDKVVVEKPQVEVKDEDVAEMLETLRKQQATWSDADVAAED
GTRATINFVGSIDGELFEGGKADNFPLEMGQGRMIPGFEDGIKGKKAGDELTINVNFPEDYHAENLKGKAAEFAITVVKV
EARELPEMNDEFVTKFGAQGGVEGLKTEVRKNMERELAQTVKNKIKEQAINGLVEQNNIDVPSALIDQEVQVLRQQAVQR
FGGNADSAPELPRELFEEQAKRRVVVGLLLGEVIKTEELKADDEKVKALINEMASAYEDPTEVVAYYEGNEQMMNNMRNV
ALEEQAIDAILAKAQVSEKAVGFNELMNQQPA
>Mature_432_residues
MQVTVETKEGLERVLTITVPAANIEDAVNAELRNIAKNRRFDGFRKGKVPMKMVAKMYGSAVRHDKMGEVMQRHFIEAIV
KDKINPAGAPTFAPVEFAEGQDLVFTATFEIYPEVTLQGLDKVVVEKPQVEVKDEDVAEMLETLRKQQATWSDADVAAED
GTRATINFVGSIDGELFEGGKADNFPLEMGQGRMIPGFEDGIKGKKAGDELTINVNFPEDYHAENLKGKAAEFAITVVKV
EARELPEMNDEFVTKFGAQGGVEGLKTEVRKNMERELAQTVKNKIKEQAINGLVEQNNIDVPSALIDQEVQVLRQQAVQR
FGGNADSAPELPRELFEEQAKRRVVVGLLLGEVIKTEELKADDEKVKALINEMASAYEDPTEVVAYYEGNEQMMNNMRNV
ALEEQAIDAILAKAQVSEKAVGFNELMNQQPA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=431, Percent_Identity=66.3573085846868, Blast_Score=587, Evalue=1e-169,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_ALISL (B6EHK2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262325.1
- GeneID:   6988646
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0817
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47861; Mature: 47861

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTVETKEGLERVLTITVPAANIEDAVNAELRNIAKNRRFDGFRKGKVPMKMVAKMYGS
CEEEEEHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AVRHDKMGEVMQRHFIEAIVKDKINPAGAPTFAPVEFAEGQDLVFTATFEIYPEVTLQGL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEEEEECCHHHHHHHH
DKVVVEKPQVEVKDEDVAEMLETLRKQQATWSDADVAAEDGTRATINFVGSIDGELFEGG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHCCC
KADNFPLEMGQGRMIPGFEDGIKGKKAGDELTINVNFPEDYHAENLKGKAAEFAITVVKV
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEE
EARELPEMNDEFVTKFGAQGGVEGLKTEVRKNMERELAQTVKNKIKEQAINGLVEQNNID
CHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPSALIDQEVQVLRQQAVQRFGGNADSAPELPRELFEEQAKRRVVVGLLLGEVIKTEELK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADDEKVKALINEMASAYEDPTEVVAYYEGNEQMMNNMRNVALEEQAIDAILAKAQVSEKA
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFNELMNQQPA
CCHHHHHCCCCC
>Mature Secondary Structure
MQVTVETKEGLERVLTITVPAANIEDAVNAELRNIAKNRRFDGFRKGKVPMKMVAKMYGS
CEEEEEHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AVRHDKMGEVMQRHFIEAIVKDKINPAGAPTFAPVEFAEGQDLVFTATFEIYPEVTLQGL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEEEEECCHHHHHHHH
DKVVVEKPQVEVKDEDVAEMLETLRKQQATWSDADVAAEDGTRATINFVGSIDGELFEGG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHCCC
KADNFPLEMGQGRMIPGFEDGIKGKKAGDELTINVNFPEDYHAENLKGKAAEFAITVVKV
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEE
EARELPEMNDEFVTKFGAQGGVEGLKTEVRKNMERELAQTVKNKIKEQAINGLVEQNNID
CHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPSALIDQEVQVLRQQAVQRFGGNADSAPELPRELFEEQAKRRVVVGLLLGEVIKTEELK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADDEKVKALINEMASAYEDPTEVVAYYEGNEQMMNNMRNVALEEQAIDAILAKAQVSEKA
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFNELMNQQPA
CCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA