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The map label for this gene is kefB [H]

Identifier: 209693906

GI number: 209693906

Start: 346636

End: 348426

Strand: Direct

Name: kefB [H]

Synonym: VSAL_I0300

Alternate gene names: 209693906

Gene position: 346636-348426 (Clockwise)

Preceding gene: 209693905

Following gene: 209693907

Centisome position: 10.42

GC content: 42.94

Gene sequence:

>1791_bases
ATGGCAGGCGATTGGCTACATACCAGTGCGATATTTTTAGCTGCTGCTGTGGTTACGGTGCCATTGGCTCAACGATTTGG
CTTAGGGGCTGTACTCGGTTATTTACTCGCGGGCATCGCTATTGGACCGTGGGGATTACAACTTATTTCAGATGTTGATG
CTATTTTGCACTTCTCTGAATTTGGCGTGGTGCTTCTTTTATTTTTAATAGGCTTGGAACTGAACCCCAATAAATTATGG
AAAATGCGAAAACCTATTATTGGTTTAGGCGGCAGTCAGGTGGTGGTGACATCGTGTGTGATCACCGCTGCAATGATGTT
TTATGGTTTGGCGTGGCAAGTCAGTGTCGTTATCGGTATGGGATTGGCTTTGTCTTCAACGGCCATTGCGTTACGAGTCA
TTGAAGAGCGGAAGTTATCTGATTCAGAAAGTGGTCAGTCAGGTTTTGCGGTGTTGTTGTTCCAAGATATTGCCGTCATT
CCTATGTTAGCGGTATTGCCAGTATTAGCGGGTGGCGCAAGTGGTAATTGGCTCGATGGATTATGGGTACTCGCCGGCGT
AATTGGTATTTTTACGTTAGGGCATTTCTTATTACGTCCATTATTTAGCTATGTTGTTAAAAGTGGCGCGCGAGAACTGT
TCACTGTCGCCGCGTTATTGCTTGTTATCGGTGTTTCGGCAGCAATGAAAGAACTTGGGTTATCCATGGCGTTAGGGGCT
TTTCTCGCTGGTGTGTTATTGGCCGAAAGTGAATTTCGTCATGAGTTAGAGGTTGCGATTGAACCTTTCAAAGGATTGTT
GTTAGGTTTGTTTTTTATTGCTGTTGGTATGTCGGTCAATTTAGGCTTATTACTTCAGTACCCAGTCGAAATAGTCATTG
CTGTTACCTTATTAGTGGCATCAAAAGGATTTCTTCTTTACGGTTTAGCTCGAACTTTTGGAACGCAAGCCAAAGCCCGA
AGCCAAATGGCGATGATTTTAAGCCAAGGGGGGGAATTTGCGTTTGTCTTGTTTACTGCGGCACAAATGGAAGGCTTGTT
GAGTAAGGAATTGACGGCGTTTTTATTAGTGGTGGTCAGTTTGTCTATGATGACGACGCCATTGCTATTGATTGCTCAAG
ATAAATGGTTTGCAAGAACATTGAACCATGAAGAAGAGATGCCGTTATCTGATTTCACCCACGCCACCCAGCCAAAAGTG
ATCATAGCCGGTTTTGGTCGATTTGGTCAGATTGTCGGTCGTTTGTTGTTTGCGAATAAAATTAAGTTAACCATTTTAGA
AGGGGATCCAAGTCAAATTCGTTTGCTGAGAAAATTTGGTTATAAAGTGTATTACGGTGATGCGACTCAAGTTGAACTAT
TAAGAGCCGCAGGAGCCGAAAAAGCAGAAGCAATCGTGATTTGTAAAACAGATCCTCAGAAGGTGATGGAGATTGTGGCG
TTATGTAAACAACATTTCCCTCACTTAAAAATATTGGCTCGTGCTCGTAGCCGAGTAGAAGCGTATGAGTTACTCAATGA
AGGGGTTGATCACTTTTCTCGAGAAACTTTTTATGGAGCCCTTGATTTAGGACGACAAACCCTCACATCTTTGGGTATGC
ATCCTTATCAGGCCAAGCGTGCAGAGTCGCATTTTAAAAAGTTAGATGTTGCTATGCTAAAAGAATTGTTACCATTGCAT
TCAGATGACAAAATTCTGGCTTCTAAAGCGAAAGCCGCTCGAATAGAGTTAGAAGAGATTTTTGGTTTGGAAATGGAAAG
TGAACGTTCAAACCAGGCTCACTGGGATTAA

Upstream 100 bases:

>100_bases
CGTCAGTGCAATATTGGGCACGTAAAATTACGATAGAAAAAAGACAGTCTTATATTAATGATTACAGAAACTGGTTATTA
AATCCTAAGGGAGAACTCTA

Downstream 100 bases:

>100_bases
TTTGCACAAGGATATTTACCGTGAAAAAAAGATTCATTGCGGGTGCAGTTTGTCCGTCATGTAAAGTGTCAGACACTCTA
CGTTGGTGGCAAGAAAGTAA

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MAGDWLHTSAIFLAAAVVTVPLAQRFGLGAVLGYLLAGIAIGPWGLQLISDVDAILHFSEFGVVLLLFLIGLELNPNKLW
KMRKPIIGLGGSQVVVTSCVITAAMMFYGLAWQVSVVIGMGLALSSTAIALRVIEERKLSDSESGQSGFAVLLFQDIAVI
PMLAVLPVLAGGASGNWLDGLWVLAGVIGIFTLGHFLLRPLFSYVVKSGARELFTVAALLLVIGVSAAMKELGLSMALGA
FLAGVLLAESEFRHELEVAIEPFKGLLLGLFFIAVGMSVNLGLLLQYPVEIVIAVTLLVASKGFLLYGLARTFGTQAKAR
SQMAMILSQGGEFAFVLFTAAQMEGLLSKELTAFLLVVVSLSMMTTPLLLIAQDKWFARTLNHEEEMPLSDFTHATQPKV
IIAGFGRFGQIVGRLLFANKIKLTILEGDPSQIRLLRKFGYKVYYGDATQVELLRAAGAEKAEAIVICKTDPQKVMEIVA
LCKQHFPHLKILARARSRVEAYELLNEGVDHFSRETFYGALDLGRQTLTSLGMHPYQAKRAESHFKKLDVAMLKELLPLH
SDDKILASKAKAARIELEEIFGLEMESERSNQAHWD

Sequences:

>Translated_596_residues
MAGDWLHTSAIFLAAAVVTVPLAQRFGLGAVLGYLLAGIAIGPWGLQLISDVDAILHFSEFGVVLLLFLIGLELNPNKLW
KMRKPIIGLGGSQVVVTSCVITAAMMFYGLAWQVSVVIGMGLALSSTAIALRVIEERKLSDSESGQSGFAVLLFQDIAVI
PMLAVLPVLAGGASGNWLDGLWVLAGVIGIFTLGHFLLRPLFSYVVKSGARELFTVAALLLVIGVSAAMKELGLSMALGA
FLAGVLLAESEFRHELEVAIEPFKGLLLGLFFIAVGMSVNLGLLLQYPVEIVIAVTLLVASKGFLLYGLARTFGTQAKAR
SQMAMILSQGGEFAFVLFTAAQMEGLLSKELTAFLLVVVSLSMMTTPLLLIAQDKWFARTLNHEEEMPLSDFTHATQPKV
IIAGFGRFGQIVGRLLFANKIKLTILEGDPSQIRLLRKFGYKVYYGDATQVELLRAAGAEKAEAIVICKTDPQKVMEIVA
LCKQHFPHLKILARARSRVEAYELLNEGVDHFSRETFYGALDLGRQTLTSLGMHPYQAKRAESHFKKLDVAMLKELLPLH
SDDKILASKAKAARIELEEIFGLEMESERSNQAHWD
>Mature_595_residues
AGDWLHTSAIFLAAAVVTVPLAQRFGLGAVLGYLLAGIAIGPWGLQLISDVDAILHFSEFGVVLLLFLIGLELNPNKLWK
MRKPIIGLGGSQVVVTSCVITAAMMFYGLAWQVSVVIGMGLALSSTAIALRVIEERKLSDSESGQSGFAVLLFQDIAVIP
MLAVLPVLAGGASGNWLDGLWVLAGVIGIFTLGHFLLRPLFSYVVKSGARELFTVAALLLVIGVSAAMKELGLSMALGAF
LAGVLLAESEFRHELEVAIEPFKGLLLGLFFIAVGMSVNLGLLLQYPVEIVIAVTLLVASKGFLLYGLARTFGTQAKARS
QMAMILSQGGEFAFVLFTAAQMEGLLSKELTAFLLVVVSLSMMTTPLLLIAQDKWFARTLNHEEEMPLSDFTHATQPKVI
IAGFGRFGQIVGRLLFANKIKLTILEGDPSQIRLLRKFGYKVYYGDATQVELLRAAGAEKAEAIVICKTDPQKVMEIVAL
CKQHFPHLKILARARSRVEAYELLNEGVDHFSRETFYGALDLGRQTLTSLGMHPYQAKRAESHFKKLDVAMLKELLPLHS
DDKILASKAKAARIELEEIFGLEMESERSNQAHWD

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=384, Percent_Identity=30.46875, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1789749, Length=596, Percent_Identity=58.3892617449664, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=589, Percent_Identity=47.0288624787776, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1786685, Length=537, Percent_Identity=28.1191806331471, Blast_Score=192, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 64844; Mature: 64712

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGDWLHTSAIFLAAAVVTVPLAQRFGLGAVLGYLLAGIAIGPWGLQLISDVDAILHFSE
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA