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The map label for this gene is spoT [H]

Identifier: 209693825

GI number: 209693825

Start: 252018

End: 254147

Strand: Direct

Name: spoT [H]

Synonym: VSAL_I0212

Alternate gene names: 209693825

Gene position: 252018-254147 (Clockwise)

Preceding gene: 209693824

Following gene: 209693833

Centisome position: 7.58

GC content: 44.13

Gene sequence:

>2130_bases
TTGGATTTATTCGATAGCCTGAAAGATGTTGCCAAAGATTATTTGCCAGAGCCTCACATTGAGGCTCTTCGTCGATCTTA
TCTGGTAGCAAAAGAGGCCCATGAAGGGCAAACTCGCTCAACGGGCGAACCTTATATCATTCACCCAGTAGCTGTGGCTC
GTATCCTTGCAGAAATGCGACTCGACCACAAAACGCTAATGGCTGCATTACTTCATGATGTCATTGAAGACACCCAAGTA
AGCAAGGAAGAACTTGCAGAGCAGTTTGGCGATACCGTTGCTGAATTAGTGGATGGCGTTTCTAAGCTGGACAAACTTAA
GTTCCGCGATAAAAAAGAAGCGCAGGCTGAAAACTTCCGAAAAATGGTCATGGCGATGGTGCAAGACATCCGCGTTATTT
TGATCAAACTTGCTGACCGTACTCATAACATGCGTACACTCGGTGCTTTGCGCCCTGACAAACGTCGTCGTATTGCTCGT
GAAACCCTCGAAATATTCTCTCCTTTGGCACATCGCCTTGGTATCCACAATATCAAAACTGAATTAGAAGAACTTGGTTT
TGAAGCGCTTTACCCTAATCGCTATCGCGTATTAAAAGAAGTGGTAAAGTCAGCTCGTGGCAACCGTAAAGAGATGATCC
AAAAAATCCACGCAGAAATTGAAGGCCGTATTGCTGAAGCTGGCATTAAAGGTCGTGTGGTTGGTCGTGAGAAGAACTTG
TTCGCCATCTATCATAAGATGAAAAATAAAGAACAGCGCTTCCACACGATTATGGATATTTACGCCTTCCGAGTCATCAC
TGATGATGTTGATACCTGTTATCGCGCATTAGGTCAGGTTCATAGTATTTATAAACCTCGCCCTGGTCGCATTAAAGATT
ACATTGCAGTCCCTAAAGCCAACGGCTACCAATCTCTTCATACGTCAATGGTTGGCCCGCATGGTGTCCCTGTTGAAGTA
CAAATTCGTACCGAAGATATGGAGCAAATGGCAAATAAAGGGGTTGCTGCACATTGGTCGTACAAAGAGGGTGATAACTC
AAGCAGTACGACAGCTCAAATTCGTGCTCAGCGTTGGATGCAAAGCCTACTTGAGCTTCAACAAAGTGCAGGTAGCTCAT
TTGAATTTATTGAAAACGTAAAGTCGGATTTATTCCCGAACGAAATTTACGTCTTCACTCCTAAAGGCCGTATTTTTGAA
CTGCCAGTAGGTGCTACTGCGGTCGATTTCGCTTATGCCGTTCACTCAGATGTTGGTAATAGCTGTGTTGGCTCTCGTGT
AAACCGTCAACCTTATCCATTAAGTAAACCTCTGAAAAGTGGCCAAACGGTTGAGATAATTAGTGCTCCAGGTGCGCGTC
CAAACGCGGCTTGGTTAAACTATGTGGTTACCTCTAAAGCTCGAACTAAGATCCGTCAAGTGCTTAAAACAATGCGCAAA
GAAGACTCTGTTATTTTGGGTCGTCGCCTATTGAATCATGCATTGGGGCATAATTTATCTTTAGATGATGTTTCTGAAGA
AAACATGACGCACGTTTTATCGGATCTTAAGCTAGCTACACAAGACGATTTATTAGCGGCGATTGGTCTAGGCGAATTAA
TGAGTATTGTGGTTGCTCGCCGATTGCTTGGTGATGCCGACGAATTGACCCAACAAAACCCGTCTAACATTGAAACCTCG
ACAGGTAAGAAATTACCCATTCGCGGTGCTGATGGAATACTGTTGACCTTTGCTAACTGTTGTCGCCCTATTCCTGGTGA
TCATATCATTGCTCATGTTAGTCCTGGTCGTGGTCTTGTCGTTCATATGGAAGACTGTGCCAATGTTCGTGGTTATCAAA
AAGAATTAGATAAATACATGGGCGTTGAATGGGCGGACGAATTTGATCAAGAATTTGCAACCGCTCTAGATATTGATCTG
CAAAACCATCAAGGTGCGCTGGCCAATTTAACCAATGTTGTCGCGAGTACGGGATCGAATATCCGTGGTATTTCTACCGA
AGAAAAAGATGGCCGATTGTATACAGTCACCATCGAATTAACGGCGAAAGATCGCACACATTTAGCGGAAATAATGCGTC
GCATTCGAGTCATGCCGCACGCATTGAAAGTTCGTAGACATAAGAACTAG

Upstream 100 bases:

>100_bases
AGCAGCAGTAAGCTCAATCACACACAATCGTTAATCGCTATTATTTATCTTTAAGTAGATAAATACGCGTTAATAATAAG
CAAACCTGACGGGGCGAAAT

Downstream 100 bases:

>100_bases
AAGTAGATATGAGGGACGAGGTTCGAGTACGGACTTCGTCCTTTTAGTAAGAGCGATACACTAAAGCTAACTAGAACGCT
TCGCTCTAGAAACTATAAAA

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSTGEPYIIHPVAVARILAEMRLDHKTLMAALLHDVIEDTQV
SKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFRKMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIFSPLAHRLGIHNIKTELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHAEIEGRIAEAGIKGRVVGREKNL
FAIYHKMKNKEQRFHTIMDIYAFRVITDDVDTCYRALGQVHSIYKPRPGRIKDYIAVPKANGYQSLHTSMVGPHGVPVEV
QIRTEDMEQMANKGVAAHWSYKEGDNSSSTTAQIRAQRWMQSLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFE
LPVGATAVDFAYAVHSDVGNSCVGSRVNRQPYPLSKPLKSGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRK
EDSVILGRRLLNHALGHNLSLDDVSEENMTHVLSDLKLATQDDLLAAIGLGELMSIVVARRLLGDADELTQQNPSNIETS
TGKKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLVVHMEDCANVRGYQKELDKYMGVEWADEFDQEFATALDIDL
QNHQGALANLTNVVASTGSNIRGISTEEKDGRLYTVTIELTAKDRTHLAEIMRRIRVMPHALKVRRHKN

Sequences:

>Translated_709_residues
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSTGEPYIIHPVAVARILAEMRLDHKTLMAALLHDVIEDTQV
SKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFRKMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIFSPLAHRLGIHNIKTELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHAEIEGRIAEAGIKGRVVGREKNL
FAIYHKMKNKEQRFHTIMDIYAFRVITDDVDTCYRALGQVHSIYKPRPGRIKDYIAVPKANGYQSLHTSMVGPHGVPVEV
QIRTEDMEQMANKGVAAHWSYKEGDNSSSTTAQIRAQRWMQSLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFE
LPVGATAVDFAYAVHSDVGNSCVGSRVNRQPYPLSKPLKSGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRK
EDSVILGRRLLNHALGHNLSLDDVSEENMTHVLSDLKLATQDDLLAAIGLGELMSIVVARRLLGDADELTQQNPSNIETS
TGKKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLVVHMEDCANVRGYQKELDKYMGVEWADEFDQEFATALDIDL
QNHQGALANLTNVVASTGSNIRGISTEEKDGRLYTVTIELTAKDRTHLAEIMRRIRVMPHALKVRRHKN
>Mature_709_residues
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSTGEPYIIHPVAVARILAEMRLDHKTLMAALLHDVIEDTQV
SKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFRKMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIFSPLAHRLGIHNIKTELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHAEIEGRIAEAGIKGRVVGREKNL
FAIYHKMKNKEQRFHTIMDIYAFRVITDDVDTCYRALGQVHSIYKPRPGRIKDYIAVPKANGYQSLHTSMVGPHGVPVEV
QIRTEDMEQMANKGVAAHWSYKEGDNSSSTTAQIRAQRWMQSLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFE
LPVGATAVDFAYAVHSDVGNSCVGSRVNRQPYPLSKPLKSGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRK
EDSVILGRRLLNHALGHNLSLDDVSEENMTHVLSDLKLATQDDLLAAIGLGELMSIVVARRLLGDADELTQQNPSNIETS
TGKKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLVVHMEDCANVRGYQKELDKYMGVEWADEFDQEFATALDIDL
QNHQGALANLTNVVASTGSNIRGISTEEKDGRLYTVTIELTAKDRTHLAEIMRRIRVMPHALKVRRHKN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=710, Percent_Identity=73.0985915492958, Blast_Score=1085, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=688, Percent_Identity=31.9767441860465, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25143118, Length=139, Percent_Identity=33.0935251798561, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79785; Mature: 79785

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSTGEPYIIHPVAVARILAEMR
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHH
LDHKTLMAALLHDVIEDTQVSKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIFSPLAHRLGIHNIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHAEIEGRIAEAGIKGRVVGREKNL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCH
FAIYHKMKNKEQRFHTIMDIYAFRVITDDVDTCYRALGQVHSIYKPRPGRIKDYIAVPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMVGPHGVPVEVQIRTEDMEQMANKGVAAHWSYKEGDNSSSTTAQIRAQRWM
CCHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHH
QSLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFELPVGATAVDFAYAVHSDVGN
HHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHCCC
SCVGSRVNRQPYPLSKPLKSGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRK
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDSVILGRRLLNHALGHNLSLDDVSEENMTHVLSDLKLATQDDLLAAIGLGELMSIVVAR
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RLLGDADELTQQNPSNIETSTGKKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLV
HHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEE
VHMEDCANVRGYQKELDKYMGVEWADEFDQEFATALDIDLQNHQGALANLTNVVASTGSN
EEEHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCC
IRGISTEEKDGRLYTVTIELTAKDRTHLAEIMRRIRVMPHALKVRRHKN
CCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSTGEPYIIHPVAVARILAEMR
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHH
LDHKTLMAALLHDVIEDTQVSKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIFSPLAHRLGIHNIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHAEIEGRIAEAGIKGRVVGREKNL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCH
FAIYHKMKNKEQRFHTIMDIYAFRVITDDVDTCYRALGQVHSIYKPRPGRIKDYIAVPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMVGPHGVPVEVQIRTEDMEQMANKGVAAHWSYKEGDNSSSTTAQIRAQRWM
CCHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHH
QSLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFELPVGATAVDFAYAVHSDVGN
HHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHCCC
SCVGSRVNRQPYPLSKPLKSGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRK
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDSVILGRRLLNHALGHNLSLDDVSEENMTHVLSDLKLATQDDLLAAIGLGELMSIVVAR
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RLLGDADELTQQNPSNIETSTGKKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLV
HHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEE
VHMEDCANVRGYQKELDKYMGVEWADEFDQEFATALDIDLQNHQGALANLTNVVASTGSN
EEEHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCC
IRGISTEEKDGRLYTVTIELTAKDRTHLAEIMRRIRVMPHALKVRRHKN
CCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]