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The map label for this gene is dppA [H]

Identifier: 209693718

GI number: 209693718

Start: 128498

End: 130051

Strand: Direct

Name: dppA [H]

Synonym: VSAL_I0095

Alternate gene names: 209693718

Gene position: 128498-130051 (Clockwise)

Preceding gene: 209693716

Following gene: 209693719

Centisome position: 3.86

GC content: 41.76

Gene sequence:

>1554_bases
ATGAAAACCATTAAGACTAAATTAGCGATCGCGATTATGGCTGCAGGCTTAAGCTTTAGTGCTGCTGCGGCAGATATTAC
GGTAGCTTATGATGCTGATCCAGTATCTCTTGACCCACATGAGCAACTATCAGGCGGTACACTGCAACTGTCTCACATGG
TGTTTGATCCCCTAGTTCGTTATACGCAAGAGTTCGACTTTGAACCTCGTTTGGCTGAAAAATGGGAACGTGTCGATGAC
AAAACGTTCCGTTTTACGTTACGTAAAGGTGTGAAGTTCCATTCTGGCAATACAATGACTGCAGATGATGTGGTATGGAC
ATTTAATCGTCTTAAAGACTCTGCGGATTTTAAAGCGATTTTCGCACCGTATGAAAAATTAGTAAAAATTGATGATTACA
CCGTAGAACTTATTTCTAAAGGCGCTTATCCGTTGGTTCTTCAAACCGCGACTTACCTTTTCCCTATGGATAGCAAGTTC
TACTCAGGAAAAACTGAAGATGGTAAAGATAAAGATGAAATCGTAAAACACGGTAATTCATTTACTTCAACGCACATCTC
TGGTACTGGCCCATTTGTTGTTAAGTCTCGTGAACAAGGCGTGAAAGTCGTGTTTGATCGTTTTGACGGTTACTGGGATA
AAAATTCTCCAGGTAACGTCGATGAGCTGACTTTGGTTCCAATTAAAGAAAATGCAACACGTGTTGCTGCGCTTCTTTCT
GGTGACGTAGACGTTATCGCCCCTGTGGCACCGAATGATCACAAGCGAGTGAAAAATACGAAGGGTATCGATTTAGTTAC
ATTACCTGGTACTCGTCTAATTTCATTCCAAATGAATCAAACAAGCAACGAAGCATTAAAAGATGTACGTGTTCGTCAAG
CTATCGTTCATGCAATCAATAACGAAGGTATCGTTAAGAAAATCATGAAAGGCTTTGCGACAGCGGCCGGTCAGCAATCT
CCTCCAGGTTATTCTGGCTATGATGCTGATTTGGTTCCACGTTATGATCTTAAAAAAGCAAAAGAGTTGATGAAAGAAGC
GGGTTACGAGAATGGCTTCAAACTTAGCATGATGGCGCCAAATAACCGTTATGTGAATGATGCAAAAATCGCTCAAGCTG
CGGCAGCAATGCTGTCTAAGATCGGAATCAAAGTGGATCTTAAAACGTTGCCAAAAGCACAATACTGGCCTGAATTTGAT
GTTTGTTCTGCGGACATGATGATGATCGGTTGGCACTCAGATACAGAAGATTCAGCGAACCTTACTGAATTCTTAACGAT
GACTCGTAACGAAGAAACGGGTAAAGGTCAGTATAACTGTGGCCATTATTCAAACCCTGAAGTTGATCGTCTAATTGAAG
CGGCAAACGTAGAAACGGATCCAGCGAAACGCAGTGAGATGCTGAAAACAGTGGAAGACACGTTGTATAACGACGCGGCG
TTTGTTCCTCTGCATTGGCAAAATCTTGCGTGGGGTGCAAAATCAAATATTGATATTCAACCAATTGTTAACTCGATGGA
ATACCCATACTTTGGTGACTTAGTTGTTAAGTAA

Upstream 100 bases:

>100_bases
AATTTAAAGGGTTAAGAGAAGGGTAACAACGGTGAAAACCGTCTAATAATAAATAAAATACAACAAACTAGGGATTGATT
CCCGCATAAGGCGTTATGCA

Downstream 100 bases:

>100_bases
GTACTAACTATATAATCAATTAGGTTGCTTTGAATGAGTCAAAGCAACCTTTGTTTATAATAAAAAGCAAGACCAACAAT
ATGTTAGGCATGAAATTTTA

Product: transport protein, extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKTIKTKLAIAIMAAGLSFSAAAADITVAYDADPVSLDPHEQLSGGTLQLSHMVFDPLVRYTQEFDFEPRLAEKWERVDD
KTFRFTLRKGVKFHSGNTMTADDVVWTFNRLKDSADFKAIFAPYEKLVKIDDYTVELISKGAYPLVLQTATYLFPMDSKF
YSGKTEDGKDKDEIVKHGNSFTSTHISGTGPFVVKSREQGVKVVFDRFDGYWDKNSPGNVDELTLVPIKENATRVAALLS
GDVDVIAPVAPNDHKRVKNTKGIDLVTLPGTRLISFQMNQTSNEALKDVRVRQAIVHAINNEGIVKKIMKGFATAAGQQS
PPGYSGYDADLVPRYDLKKAKELMKEAGYENGFKLSMMAPNNRYVNDAKIAQAAAAMLSKIGIKVDLKTLPKAQYWPEFD
VCSADMMMIGWHSDTEDSANLTEFLTMTRNEETGKGQYNCGHYSNPEVDRLIEAANVETDPAKRSEMLKTVEDTLYNDAA
FVPLHWQNLAWGAKSNIDIQPIVNSMEYPYFGDLVVK

Sequences:

>Translated_517_residues
MKTIKTKLAIAIMAAGLSFSAAAADITVAYDADPVSLDPHEQLSGGTLQLSHMVFDPLVRYTQEFDFEPRLAEKWERVDD
KTFRFTLRKGVKFHSGNTMTADDVVWTFNRLKDSADFKAIFAPYEKLVKIDDYTVELISKGAYPLVLQTATYLFPMDSKF
YSGKTEDGKDKDEIVKHGNSFTSTHISGTGPFVVKSREQGVKVVFDRFDGYWDKNSPGNVDELTLVPIKENATRVAALLS
GDVDVIAPVAPNDHKRVKNTKGIDLVTLPGTRLISFQMNQTSNEALKDVRVRQAIVHAINNEGIVKKIMKGFATAAGQQS
PPGYSGYDADLVPRYDLKKAKELMKEAGYENGFKLSMMAPNNRYVNDAKIAQAAAAMLSKIGIKVDLKTLPKAQYWPEFD
VCSADMMMIGWHSDTEDSANLTEFLTMTRNEETGKGQYNCGHYSNPEVDRLIEAANVETDPAKRSEMLKTVEDTLYNDAA
FVPLHWQNLAWGAKSNIDIQPIVNSMEYPYFGDLVVK
>Mature_517_residues
MKTIKTKLAIAIMAAGLSFSAAAADITVAYDADPVSLDPHEQLSGGTLQLSHMVFDPLVRYTQEFDFEPRLAEKWERVDD
KTFRFTLRKGVKFHSGNTMTADDVVWTFNRLKDSADFKAIFAPYEKLVKIDDYTVELISKGAYPLVLQTATYLFPMDSKF
YSGKTEDGKDKDEIVKHGNSFTSTHISGTGPFVVKSREQGVKVVFDRFDGYWDKNSPGNVDELTLVPIKENATRVAALLS
GDVDVIAPVAPNDHKRVKNTKGIDLVTLPGTRLISFQMNQTSNEALKDVRVRQAIVHAINNEGIVKKIMKGFATAAGQQS
PPGYSGYDADLVPRYDLKKAKELMKEAGYENGFKLSMMAPNNRYVNDAKIAQAAAAMLSKIGIKVDLKTLPKAQYWPEFD
VCSADMMMIGWHSDTEDSANLTEFLTMTRNEETGKGQYNCGHYSNPEVDRLIEAANVETDPAKRSEMLKTVEDTLYNDAA
FVPLHWQNLAWGAKSNIDIQPIVNSMEYPYFGDLVVK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=505, Percent_Identity=28.1188118811881, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1787052, Length=487, Percent_Identity=26.694045174538, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87081878, Length=523, Percent_Identity=24.282982791587, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787762, Length=509, Percent_Identity=25.147347740668, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787551, Length=450, Percent_Identity=25.5555555555556, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787495, Length=518, Percent_Identity=25.6756756756757, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789397, Length=520, Percent_Identity=24.2307692307692, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789887, Length=543, Percent_Identity=23.2044198895028, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57629; Mature: 57629

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIKTKLAIAIMAAGLSFSAAAADITVAYDADPVSLDPHEQLSGGTLQLSHMVFDPLVR
CCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCEEEEHHHHHHHHHH
YTQEFDFEPRLAEKWERVDDKTFRFTLRKGVKFHSGNTMTADDVVWTFNRLKDSADFKAI
HHHHCCCCCHHHHHHHHCCCHHEEEEECCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHH
FAPYEKLVKIDDYTVELISKGAYPLVLQTATYLFPMDSKFYSGKTEDGKDKDEIVKHGNS
HHCHHHHEECCCHHHHHHHCCCCEEEEEHHHHEEECCCCCCCCCCCCCCCHHHHHHCCCC
FTSTHISGTGPFVVKSREQGVKVVFDRFDGYWDKNSPGNVDELTLVPIKENATRVAALLS
CCEEEECCCCCEEEECCCCCCEEEEEHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHC
GDVDVIAPVAPNDHKRVKNTKGIDLVTLPGTRLISFQMNQTSNEALKDVRVRQAIVHAIN
CCCEEEECCCCCCHHHCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHC
NEGIVKKIMKGFATAAGQQSPPGYSGYDADLVPRYDLKKAKELMKEAGYENGFKLSMMAP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECC
NNRYVNDAKIAQAAAAMLSKIGIKVDLKTLPKAQYWPEFDVCSADMMMIGWHSDTEDSAN
CCCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
LTEFLTMTRNEETGKGQYNCGHYSNPEVDRLIEAANVETDPAKRSEMLKTVEDTLYNDAA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEE
FVPLHWQNLAWGAKSNIDIQPIVNSMEYPYFGDLVVK
EEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCEEEC
>Mature Secondary Structure
MKTIKTKLAIAIMAAGLSFSAAAADITVAYDADPVSLDPHEQLSGGTLQLSHMVFDPLVR
CCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCEEEEHHHHHHHHHH
YTQEFDFEPRLAEKWERVDDKTFRFTLRKGVKFHSGNTMTADDVVWTFNRLKDSADFKAI
HHHHCCCCCHHHHHHHHCCCHHEEEEECCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHH
FAPYEKLVKIDDYTVELISKGAYPLVLQTATYLFPMDSKFYSGKTEDGKDKDEIVKHGNS
HHCHHHHEECCCHHHHHHHCCCCEEEEEHHHHEEECCCCCCCCCCCCCCCHHHHHHCCCC
FTSTHISGTGPFVVKSREQGVKVVFDRFDGYWDKNSPGNVDELTLVPIKENATRVAALLS
CCEEEECCCCCEEEECCCCCCEEEEEHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHC
GDVDVIAPVAPNDHKRVKNTKGIDLVTLPGTRLISFQMNQTSNEALKDVRVRQAIVHAIN
CCCEEEECCCCCCHHHCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHC
NEGIVKKIMKGFATAAGQQSPPGYSGYDADLVPRYDLKKAKELMKEAGYENGFKLSMMAP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECC
NNRYVNDAKIAQAAAAMLSKIGIKVDLKTLPKAQYWPEFDVCSADMMMIGWHSDTEDSAN
CCCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
LTEFLTMTRNEETGKGQYNCGHYSNPEVDRLIEAANVETDPAKRSEMLKTVEDTLYNDAA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEE
FVPLHWQNLAWGAKSNIDIQPIVNSMEYPYFGDLVVK
EEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]