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The map label for this gene is potB [H]

Identifier: 203284539

GI number: 203284539

Start: 691237

End: 692040

Strand: Reverse

Name: potB [H]

Synonym: BDU_644

Alternate gene names: 203284539

Gene position: 692040-691237 (Counterclockwise)

Preceding gene: 203284540

Following gene: 203284538

Centisome position: 74.28

GC content: 25.12

Gene sequence:

>804_bases
ATGAATAAAATAATGTTAACATTATATAGTCTCTTCTTATTAATCTTCATTATCTTCCCACTAGTCATAATAATAACTCT
AGGGTTTCTTAATGAAAGAAACGAATTTACATTTGCAAATTTTATAAGATTATTAGAACCAAGTTACTTAAGAATTTTTT
CAAGAAGTATAAATTTTGCATTAATTACAACAATTTTTTGCATATTAATTGGATATCCTACAGCATGGTTTATATCTATG
TCAAAAAAAAGTACTCAAAACAATTTAATTATAATGATAATACTCCCTATGTGGATTAATACCCTACTTAGAAGTTATGC
TTGGATTAGAATACTTGGAAAAAATGGCATTATCAATAATTTATTTATAGCAATGGGATTTAAACCCATGGATTTACTTT
ACAATGAAAAAGCTGTAATAATTGGTATGATATACAATTTTTTACCATTTATGATTTTACCAATATATATAGGGCTCTCA
AAAATAAAATATGAATACATCGAAGCAGCAAGGGACCTAGGAGCTAGGATGTGGCAAATATTAATCTATATCAAAATACC
ATTAACATTATCATATCTTGCTACAGGAATAATAATGGTATTTATTCCTTCAATCACAGTGTTCATTATCTCAGATCTTC
TAGGAGGTTCTAAACAAATTTTACTTGGAAATTTAATTGAAAAACAATTTTTATTTGTACCAGATTGGAATACAGGATCT
GCTATATCATTTATTTTAATGATAGTCATAGTAATATTTAATTTAACAATAATTAAATTAATGCAAAAAACAGAAAAAAT
ATGA

Upstream 100 bases:

>100_bases
ATTGGTTAGTCCAAAGTACAAAATTGACAAAAGTTGGTGAAGAAGTTGACATTTCACTAGGCCCTGATGACATTCATGTA
ATGTGTAAGGAATAATAAGT

Downstream 100 bases:

>100_bases
GGTATTAACATGCTTAAAATATTTAAAAATACTTTTTTATTTTTGACACTTAGCTTTATTTATGCTCCCATAATAATTTT
AATAATTTATTCTTTCAACG

Product: spermidine/putrescine ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNKIMLTLYSLFLLIFIIFPLVIIITLGFLNERNEFTFANFIRLLEPSYLRIFSRSINFALITTIFCILIGYPTAWFISM
SKKSTQNNLIIMIILPMWINTLLRSYAWIRILGKNGIINNLFIAMGFKPMDLLYNEKAVIIGMIYNFLPFMILPIYIGLS
KIKYEYIEAARDLGARMWQILIYIKIPLTLSYLATGIIMVFIPSITVFIISDLLGGSKQILLGNLIEKQFLFVPDWNTGS
AISFILMIVIVIFNLTIIKLMQKTEKI

Sequences:

>Translated_267_residues
MNKIMLTLYSLFLLIFIIFPLVIIITLGFLNERNEFTFANFIRLLEPSYLRIFSRSINFALITTIFCILIGYPTAWFISM
SKKSTQNNLIIMIILPMWINTLLRSYAWIRILGKNGIINNLFIAMGFKPMDLLYNEKAVIIGMIYNFLPFMILPIYIGLS
KIKYEYIEAARDLGARMWQILIYIKIPLTLSYLATGIIMVFIPSITVFIISDLLGGSKQILLGNLIEKQFLFVPDWNTGS
AISFILMIVIVIFNLTIIKLMQKTEKI
>Mature_267_residues
MNKIMLTLYSLFLLIFIIFPLVIIITLGFLNERNEFTFANFIRLLEPSYLRIFSRSINFALITTIFCILIGYPTAWFISM
SKKSTQNNLIIMIILPMWINTLLRSYAWIRILGKNGIINNLFIAMGFKPMDLLYNEKAVIIGMIYNFLPFMILPIYIGLS
KIKYEYIEAARDLGARMWQILIYIKIPLTLSYLATGIIMVFIPSITVFIISDLLGGSKQILLGNLIEKQFLFVPDWNTGS
AISFILMIVIVIFNLTIIKLMQKTEKI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=247, Percent_Identity=38.0566801619433, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1787080, Length=229, Percent_Identity=38.8646288209607, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787713, Length=233, Percent_Identity=26.1802575107296, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30855; Mature: 30855

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIMLTLYSLFLLIFIIFPLVIIITLGFLNERNEFTFANFIRLLEPSYLRIFSRSINFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHCCCHH
LITTIFCILIGYPTAWFISMSKKSTQNNLIIMIILPMWINTLLRSYAWIRILGKNGIINN
HHHHHHHHHHCCCHHHEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHEEEECCCCCHHH
LFIAMGFKPMDLLYNEKAVIIGMIYNFLPFMILPIYIGLSKIKYEYIEAARDLGARMWQI
HHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIYIKIPLTLSYLATGIIMVFIPSITVFIISDLLGGSKQILLGNLIEKQFLFVPDWNTGS
HHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCCH
AISFILMIVIVIFNLTIIKLMQKTEKI
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIMLTLYSLFLLIFIIFPLVIIITLGFLNERNEFTFANFIRLLEPSYLRIFSRSINFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHCCCHH
LITTIFCILIGYPTAWFISMSKKSTQNNLIIMIILPMWINTLLRSYAWIRILGKNGIINN
HHHHHHHHHHCCCHHHEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHEEEECCCCCHHH
LFIAMGFKPMDLLYNEKAVIIGMIYNFLPFMILPIYIGLSKIKYEYIEAARDLGARMWQI
HHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIYIKIPLTLSYLATGIIMVFIPSITVFIISDLLGGSKQILLGNLIEKQFLFVPDWNTGS
HHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCCH
AISFILMIVIVIFNLTIIKLMQKTEKI
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]