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The map label for this gene is cht60 [H]

Identifier: 203284521

GI number: 203284521

Start: 659283

End: 660938

Strand: Reverse

Name: cht60 [H]

Synonym: BDU_623

Alternate gene names: 203284521

Gene position: 660938-659283 (Counterclockwise)

Preceding gene: 203284523

Following gene: 203284519

Centisome position: 70.94

GC content: 25.36

Gene sequence:

>1656_bases
ATGAAAAAAATTATTTATATAATTGTTTTTTTAATTAATAATAATTGTTTTTGTTATGACTTTTGGAGTACAGAAAATAA
AGAAAAACTAATAGAACAAATAGTAAGTCAAATGAATGACAAAGAATTATTGGGACAAATGTTTATGATAAGTTATCCAA
AAGAAAAAATAACAAACTTCACCCTAAACTTCATCAAAGAAAAAAATTTAGGTGGAATCAAAATTTTTGGATGGAATGCA
AAAAATTTATACAACTTAATAGAAAGTATAAATAAAGCACAAACAATTTCTCAAAAGAATAGATTTAAAATTCCCTTATT
TATAGCAACAGATCAAGAAGGTGGATGGACACAACATATAAAACTTAAAACATCAAAAACAATAGGAAATCTTGGAATCA
CAGCAACTTTATCACCAAATGATGCCTACTTTACTGGATATCATATAGCAAATGAATTAAAACAACTTGGAATTAACATA
AATTTTGCCCCAATAACAGATATTTACAGTAATGAAGAAAATTTCATAATAGGCCCAAGAACATATTCAAATAATCCACA
AATCGTATCTCTATTTGCATTGGCTTTCTATAAAGGACAAAAACAAGCAGGCATCATCTCAACAGCAAAACATTTTCCCG
GACATGGCAATACTGTTATTGATTCTCATATAAAAATGCCAATAATCAATTCCGATTTAAAAGAAATTAAAACAAATGAA
TTGCTGCCATATAAAATACTAATACAAGAAAACATTCCAATGATTATGAGCGGACACTTAGCATATCCAATGCTTACAAA
CGGAAAAGAAATACCTGCATCCTCATCAACTAAAATTATCAAGGAAATTCTTAGGCAAGACTTAAAATACGACAATTTAA
TAATAACAGACGACCTACTAATGAATGCAGTAAGATATCAAAATGAAAGCATTTATGACACAATTGAACGAATTATTAGA
ACAGAAACTGATATTTTATTAATATCATTAAATGAAGATATACAAAATAATGCTTACAATCAACTATTAAACCTCATGCA
AAAAGACAATACAATAAAAGAAAACATTATTAAATCTAACAAAAGAATACTTAGAATAAAATTAGAATATCTAAAAGAAA
AAAGAAACAAAGAAGAACTTTTCCCAAAATATGACAATGCTAAAGAAATACCTACTAAAGAAGCTGAATTTTTTTTTGAA
CAAAATACATTAAGAAGTATAACAAAAATAAAACTATCAAAAAAAATATCAAAGCATAAAAAAACGCTATTAATATCACC
TTATAATACAATGATTAAAGAAGGTAAAAAAATATTTCAAAATAGTTATGGCTATTATTATAATTATTATCCACTAAATA
GCATAAATACCACACAATTAAAAGAAATAAAAAATTTAATTGCACAATATGAACAAGTTATTTTTAATGTATCAACACCT
GCTAGTTTAGAGTACATAAAAAATTTAAAAGAATATAAAAATAAAATAAGCATCATTGTATCTCTTACCCCCCAACACAT
TAAACAACTGAACTGGATAGAAAACATAGTAGTAGTATATGGAACTACAACACTATCATTTAAATCTGGATTTTTAACTT
TAACAGAAGATTTTAATCCAAAAGGACAAATACCTTTAATGAATCTCAAGAATTAA

Upstream 100 bases:

>100_bases
ATATATTCAAAAAATTTTAAAATAATAAGGAACTTGAAATAAGTAAACTTAAAACAATATGATATACTTATGAATCAAAA
AAAGATTAGTATGGTTTCAA

Downstream 100 bases:

>100_bases
TAGCATCTTTAATATATGCAATTATTTGATTTTCTAAAAGGTTTAAAGCAGTATTTTTAATCTTAAATCCACTAGCATGT
TTATGTCCCCCTCCTCCAAA

Product: beta-glucosidase, putative

Products: NA

Alternate protein names: Beta-N-acetylhexosaminidase; Chitobiase; N-acetyl-beta-glucosaminidase [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MKKIIYIIVFLINNNCFCYDFWSTENKEKLIEQIVSQMNDKELLGQMFMISYPKEKITNFTLNFIKEKNLGGIKIFGWNA
KNLYNLIESINKAQTISQKNRFKIPLFIATDQEGGWTQHIKLKTSKTIGNLGITATLSPNDAYFTGYHIANELKQLGINI
NFAPITDIYSNEENFIIGPRTYSNNPQIVSLFALAFYKGQKQAGIISTAKHFPGHGNTVIDSHIKMPIINSDLKEIKTNE
LLPYKILIQENIPMIMSGHLAYPMLTNGKEIPASSSTKIIKEILRQDLKYDNLIITDDLLMNAVRYQNESIYDTIERIIR
TETDILLISLNEDIQNNAYNQLLNLMQKDNTIKENIIKSNKRILRIKLEYLKEKRNKEELFPKYDNAKEIPTKEAEFFFE
QNTLRSITKIKLSKKISKHKKTLLISPYNTMIKEGKKIFQNSYGYYYNYYPLNSINTTQLKEIKNLIAQYEQVIFNVSTP
ASLEYIKNLKEYKNKISIIVSLTPQHIKQLNWIENIVVVYGTTTLSFKSGFLTLTEDFNPKGQIPLMNLKN

Sequences:

>Translated_551_residues
MKKIIYIIVFLINNNCFCYDFWSTENKEKLIEQIVSQMNDKELLGQMFMISYPKEKITNFTLNFIKEKNLGGIKIFGWNA
KNLYNLIESINKAQTISQKNRFKIPLFIATDQEGGWTQHIKLKTSKTIGNLGITATLSPNDAYFTGYHIANELKQLGINI
NFAPITDIYSNEENFIIGPRTYSNNPQIVSLFALAFYKGQKQAGIISTAKHFPGHGNTVIDSHIKMPIINSDLKEIKTNE
LLPYKILIQENIPMIMSGHLAYPMLTNGKEIPASSSTKIIKEILRQDLKYDNLIITDDLLMNAVRYQNESIYDTIERIIR
TETDILLISLNEDIQNNAYNQLLNLMQKDNTIKENIIKSNKRILRIKLEYLKEKRNKEELFPKYDNAKEIPTKEAEFFFE
QNTLRSITKIKLSKKISKHKKTLLISPYNTMIKEGKKIFQNSYGYYYNYYPLNSINTTQLKEIKNLIAQYEQVIFNVSTP
ASLEYIKNLKEYKNKISIIVSLTPQHIKQLNWIENIVVVYGTTTLSFKSGFLTLTEDFNPKGQIPLMNLKN
>Mature_551_residues
MKKIIYIIVFLINNNCFCYDFWSTENKEKLIEQIVSQMNDKELLGQMFMISYPKEKITNFTLNFIKEKNLGGIKIFGWNA
KNLYNLIESINKAQTISQKNRFKIPLFIATDQEGGWTQHIKLKTSKTIGNLGITATLSPNDAYFTGYHIANELKQLGINI
NFAPITDIYSNEENFIIGPRTYSNNPQIVSLFALAFYKGQKQAGIISTAKHFPGHGNTVIDSHIKMPIINSDLKEIKTNE
LLPYKILIQENIPMIMSGHLAYPMLTNGKEIPASSSTKIIKEILRQDLKYDNLIITDDLLMNAVRYQNESIYDTIERIIR
TETDILLISLNEDIQNNAYNQLLNLMQKDNTIKENIIKSNKRILRIKLEYLKEKRNKEELFPKYDNAKEIPTKEAEFFFE
QNTLRSITKIKLSKKISKHKKTLLISPYNTMIKEGKKIFQNSYGYYYNYYPLNSINTTQLKEIKNLIAQYEQVIFNVSTP
ASLEYIKNLKEYKNKISIIVSLTPQHIKQLNWIENIVVVYGTTTLSFKSGFLTLTEDFNPKGQIPLMNLKN

Specific function: Most active towards p-nitrophenyl-N-acetyl-beta-D- glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose [H]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=240, Percent_Identity=31.6666666666667, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3 [H]

EC number: =3.2.1.52 [H]

Molecular weight: Translated: 63842; Mature: 63842

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIYIIVFLINNNCFCYDFWSTENKEKLIEQIVSQMNDKELLGQMFMISYPKEKITNF
CCEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHEECCHHHHHHH
TLNFIKEKNLGGIKIFGWNAKNLYNLIESINKAQTISQKNRFKIPLFIATDQEGGWTQHI
HEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEE
KLKTSKTIGNLGITATLSPNDAYFTGYHIANELKQLGININFAPITDIYSNEENFIIGPR
EEEECCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCCEEEEECHHHHCCCCCEEECCE
TYSNNPQIVSLFALAFYKGQKQAGIISTAKHFPGHGNTVIDSHIKMPIINSDLKEIKTNE
EECCCCCHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCEEEECCEECCEECCHHHHHHCCC
LLPYKILIQENIPMIMSGHLAYPMLTNGKEIPASSSTKIIKEILRQDLKYDNLIITDDLL
CCCEEEEEECCCCEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHH
MNAVRYQNESIYDTIERIIRTETDILLISLNEDIQNNAYNQLLNLMQKDNTIKENIIKSN
HHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCC
KRILRIKLEYLKEKRNKEELFPKYDNAKEIPTKEAEFFFEQNTLRSITKIKLSKKISKHK
CEEEEEEHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
KTLLISPYNTMIKEGKKIFQNSYGYYYNYYPLNSINTTQLKEIKNLIAQYEQVIFNVSTP
CEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCC
ASLEYIKNLKEYKNKISIIVSLTPQHIKQLNWIENIVVVYGTTTLSFKSGFLTLTEDFNP
HHHHHHHHHHHHHCEEEEEEEECHHHHHHHHHHCCEEEEEECEEEEECCCEEEEEECCCC
KGQIPLMNLKN
CCCEEEECCCC
>Mature Secondary Structure
MKKIIYIIVFLINNNCFCYDFWSTENKEKLIEQIVSQMNDKELLGQMFMISYPKEKITNF
CCEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHEECCHHHHHHH
TLNFIKEKNLGGIKIFGWNAKNLYNLIESINKAQTISQKNRFKIPLFIATDQEGGWTQHI
HEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEE
KLKTSKTIGNLGITATLSPNDAYFTGYHIANELKQLGININFAPITDIYSNEENFIIGPR
EEEECCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCCEEEEECHHHHCCCCCEEECCE
TYSNNPQIVSLFALAFYKGQKQAGIISTAKHFPGHGNTVIDSHIKMPIINSDLKEIKTNE
EECCCCCHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCEEEECCEECCEECCHHHHHHCCC
LLPYKILIQENIPMIMSGHLAYPMLTNGKEIPASSSTKIIKEILRQDLKYDNLIITDDLL
CCCEEEEEECCCCEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHH
MNAVRYQNESIYDTIERIIRTETDILLISLNEDIQNNAYNQLLNLMQKDNTIKENIIKSN
HHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCC
KRILRIKLEYLKEKRNKEELFPKYDNAKEIPTKEAEFFFEQNTLRSITKIKLSKKISKHK
CEEEEEEHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
KTLLISPYNTMIKEGKKIFQNSYGYYYNYYPLNSINTTQLKEIKNLIAQYEQVIFNVSTP
CEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCC
ASLEYIKNLKEYKNKISIIVSLTPQHIKQLNWIENIVVVYGTTTLSFKSGFLTLTEDFNP
HHHHHHHHHHHHHCEEEEEEEECHHHHHHHHHHCCEEEEEECEEEEECCCEEEEEECCCC
KGQIPLMNLKN
CCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063094 [H]