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The map label for this gene is aspS

Identifier: 203284356

GI number: 203284356

Start: 478470

End: 480227

Strand: Reverse

Name: aspS

Synonym: BDU_445

Alternate gene names: 203284356

Gene position: 480227-478470 (Counterclockwise)

Preceding gene: 203284357

Following gene: 203284344

Centisome position: 51.54

GC content: 27.3

Gene sequence:

>1758_bases
ATGTTTAAAATCATTAAATGCAATCAAATAAATAATAAACTTATAAATAAAAAGATAGAAATAAATGGATGGGTAAAAAA
AATAAGAAATCATGGAAAAGTCACATTCATCAATATTAGAGACAGATATGATGAAGCACAAATACTTGTAAGTGATGAGA
AACTTTTAAAAATAACATCTCAAATAAAAATGGAATACTGCATTAAAATTCAAGGAAAATTAAATTTAAGACCCTTAGAA
CTTGTAAACAGAGAAATGAAAACCGGAGAACTTGAAATAATTGCAGAAAATATAGATATAATTTCAAAATGCAATGAATT
ACCCTTTATGATTGAAAACAATAATAATGCCAGTGATAACTCCAAACTTGAATATAGGTATTTAGATTTAAGACGAGAAG
AACAAAAACAAAAAATAATATTAAGAAGTAAAGTAATACATATCATTAGAAATCATCTAACAAAACAAGATTTCTTAGAA
TTAGAAACACCGACATTTGTTAAGTCAACTCCAGAAGGTGCCAGAGATTTTCTTGTTCCATCAAGAATACATAAGGGTCA
TTTTTATGCACTACCACAATCACCACAAATTTATAAACAACTTGCAATGATAGCCGGACTTGATAAATATTTTCAAATAG
CTAGATGCTACAGAGATGAAGATTCAAGAGGCGATAGACAACCAGAATTCACACAACTTGACCTTGAAATGAGTTTTATA
AAAAAAGAAAATATATTTAAACTAATAGAAAATCTTATTTTTACTATTTTTAAAAACTCATTAAATATCACATTACCAAA
AAAATTCAAAAAAATTACCTACAAAGATGCAATGAATATATATGGAAGTGACAAACCAGATACTAGATATGAATTATTAA
TACAAGACATGAGTAAAGCTTTAAAGCAATCTCCATTTGACGTCTTTAAAGACACATTACAAAACAAAGGAACAATAAAA
GCACTTATTATAAAAAATCAAGCACATAATTTCTCAAGAAGTAAGATCAATTCATTAGAAGAACATGCCAAACTTTATAA
AGCACGTACTCTTTACTTTACAAAAATAGAAAATAATGAATTTACAGGTGGCATTGCAAAATTTATAAATCCAATTAAAA
AAACTCTAATTGAAACTTATTCACTTAAAAATAATGACTTAATATTTTTTATAGCAGACCTGTGGGAAACCGCTTGTAAA
GCCATAGGGCAAATTAGAATTAAAATTGCAACCGAACTTAATCTTATCAACAAAAATATATTTGAGTTCCTATGGATTTA
TGATTTTCCTTTATTTGAATATGATGAGGATACACAAAGTTATAAAGCAGCTCATCATATGTTTTCAATGCCGCAAGCAA
AATATATAAATACTCTAGAGAGTAATCCAAGTGAAGTCTTAGGAGAAGTTTATGATCTTGTATTAAATGGAACAGAACTT
GGTTCAGGCTCAATCAGAGTACATACCAAAGAACTGCAACAAAGAATTTTTAATATAGTAGGATTTAACGATAAAATAGC
TGAAGAAAGATTTGGTTTTTTTCTAAAAGCATTAGAATATGGGGCTCCCATTCATGGAGGAATAGCAATTGGAATTGACA
GGCTTTTAATGTTAATGACAAATTCAAATTCAATAAAAGATGTAATACTTTTTCCAAAAAATTCATTTGCTGCAAGCCCA
CTTGATAAATCTCCTTCTAAAATTTCAAATGAACAGCTCAAAGAACTAAATTTAACAATTGAAAATAATAAAAATTAA

Upstream 100 bases:

>100_bases
CATTTTATCATCTATTGTAGGTAAAGTAGATTCTAATAAAAAAGCAATGCTTAAAGCTAAAACTGACAAAGAAATTTATA
ACATATTAATAGGATAGGTT

Downstream 100 bases:

>100_bases
GGATGCAAAAGCATCCTTAATTAAGGGAGCAGAAAATTTATTCAATTACAATTTTTTTCTCCTTAGATTTTTCAATAACT
TCTGAGTTTTTTGGAAGTTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MFKIIKCNQINNKLINKKIEINGWVKKIRNHGKVTFINIRDRYDEAQILVSDEKLLKITSQIKMEYCIKIQGKLNLRPLE
LVNREMKTGELEIIAENIDIISKCNELPFMIENNNNASDNSKLEYRYLDLRREEQKQKIILRSKVIHIIRNHLTKQDFLE
LETPTFVKSTPEGARDFLVPSRIHKGHFYALPQSPQIYKQLAMIAGLDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFI
KKENIFKLIENLIFTIFKNSLNITLPKKFKKITYKDAMNIYGSDKPDTRYELLIQDMSKALKQSPFDVFKDTLQNKGTIK
ALIIKNQAHNFSRSKINSLEEHAKLYKARTLYFTKIENNEFTGGIAKFINPIKKTLIETYSLKNNDLIFFIADLWETACK
AIGQIRIKIATELNLINKNIFEFLWIYDFPLFEYDEDTQSYKAAHHMFSMPQAKYINTLESNPSEVLGEVYDLVLNGTEL
GSGSIRVHTKELQQRIFNIVGFNDKIAEERFGFFLKALEYGAPIHGGIAIGIDRLLMLMTNSNSIKDVILFPKNSFAASP
LDKSPSKISNEQLKELNLTIENNKN

Sequences:

>Translated_585_residues
MFKIIKCNQINNKLINKKIEINGWVKKIRNHGKVTFINIRDRYDEAQILVSDEKLLKITSQIKMEYCIKIQGKLNLRPLE
LVNREMKTGELEIIAENIDIISKCNELPFMIENNNNASDNSKLEYRYLDLRREEQKQKIILRSKVIHIIRNHLTKQDFLE
LETPTFVKSTPEGARDFLVPSRIHKGHFYALPQSPQIYKQLAMIAGLDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFI
KKENIFKLIENLIFTIFKNSLNITLPKKFKKITYKDAMNIYGSDKPDTRYELLIQDMSKALKQSPFDVFKDTLQNKGTIK
ALIIKNQAHNFSRSKINSLEEHAKLYKARTLYFTKIENNEFTGGIAKFINPIKKTLIETYSLKNNDLIFFIADLWETACK
AIGQIRIKIATELNLINKNIFEFLWIYDFPLFEYDEDTQSYKAAHHMFSMPQAKYINTLESNPSEVLGEVYDLVLNGTEL
GSGSIRVHTKELQQRIFNIVGFNDKIAEERFGFFLKALEYGAPIHGGIAIGIDRLLMLMTNSNSIKDVILFPKNSFAASP
LDKSPSKISNEQLKELNLTIENNKN
>Mature_585_residues
MFKIIKCNQINNKLINKKIEINGWVKKIRNHGKVTFINIRDRYDEAQILVSDEKLLKITSQIKMEYCIKIQGKLNLRPLE
LVNREMKTGELEIIAENIDIISKCNELPFMIENNNNASDNSKLEYRYLDLRREEQKQKIILRSKVIHIIRNHLTKQDFLE
LETPTFVKSTPEGARDFLVPSRIHKGHFYALPQSPQIYKQLAMIAGLDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFI
KKENIFKLIENLIFTIFKNSLNITLPKKFKKITYKDAMNIYGSDKPDTRYELLIQDMSKALKQSPFDVFKDTLQNKGTIK
ALIIKNQAHNFSRSKINSLEEHAKLYKARTLYFTKIENNEFTGGIAKFINPIKKTLIETYSLKNNDLIFFIADLWETACK
AIGQIRIKIATELNLINKNIFEFLWIYDFPLFEYDEDTQSYKAAHHMFSMPQAKYINTLESNPSEVLGEVYDLVLNGTEL
GSGSIRVHTKELQQRIFNIVGFNDKIAEERFGFFLKALEYGAPIHGGIAIGIDRLLMLMTNSNSIKDVILFPKNSFAASP
LDKSPSKISNEQLKELNLTIENNKN

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=589, Percent_Identity=36.5025466893039, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI45439306, Length=279, Percent_Identity=23.6559139784946, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI194272210, Length=262, Percent_Identity=25.5725190839695, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI5031815, Length=262, Percent_Identity=25.5725190839695, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4758762, Length=284, Percent_Identity=26.056338028169, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI226491502, Length=279, Percent_Identity=22.9390681003584, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788173, Length=592, Percent_Identity=38.1756756756757, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1790571, Length=312, Percent_Identity=25.9615384615385, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789256, Length=309, Percent_Identity=25.5663430420712, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=602, Percent_Identity=32.5581395348837, Blast_Score=317, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17551876, Length=287, Percent_Identity=25.7839721254355, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=255, Percent_Identity=22.7450980392157, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=255, Percent_Identity=22.7450980392157, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=255, Percent_Identity=22.7450980392157, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71984122, Length=284, Percent_Identity=23.943661971831, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=632, Percent_Identity=33.7025316455696, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6323011, Length=280, Percent_Identity=26.4285714285714, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=291, Percent_Identity=23.7113402061856, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584738, Length=590, Percent_Identity=35.4237288135593, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17136276, Length=278, Percent_Identity=28.4172661870504, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=25.8823529411765, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=25.8823529411765, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921528, Length=234, Percent_Identity=26.0683760683761, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_BORDL (B5RM04)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002222096.1
- EnsemblBacteria:   EBBORT00000001509
- GeneID:   6918529
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_445
- GeneTree:   EBGT00050000007238
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 68004; Mature: 68004

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIIKCNQINNKLINKKIEINGWVKKIRNHGKVTFINIRDRYDEAQILVSDEKLLKITS
CEEEEEECCCCHHHCCCEEEHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCHHHHHHHH
QIKMEYCIKIQGKLNLRPLELVNREMKTGELEIIAENIDIISKCNELPFMIENNNNASDN
HHCEEEEEEEECCCCCCHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCEEEECCCCCCCC
SKLEYRYLDLRREEQKQKIILRSKVIHIIRNHLTKQDFLELETPTFVKSTPEGARDFLVP
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHCCCC
SRIHKGHFYALPQSPQIYKQLAMIAGLDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFI
CCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHH
KKENIFKLIENLIFTIFKNSLNITLPKKFKKITYKDAMNIYGSDKPDTRYELLIQDMSKA
HHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LKQSPFDVFKDTLQNKGTIKALIIKNQAHNFSRSKINSLEEHAKLYKARTLYFTKIENNE
HHCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCC
FTGGIAKFINPIKKTLIETYSLKNNDLIFFIADLWETACKAIGQIRIKIATELNLINKNI
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHEEEEEEEHHHHHHHHH
FEFLWIYDFPLFEYDEDTQSYKAAHHMFSMPQAKYINTLESNPSEVLGEVYDLVLNGTEL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEE
GSGSIRVHTKELQQRIFNIVGFNDKIAEERFGFFLKALEYGAPIHGGIAIGIDRLLMLMT
CCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEE
NSNSIKDVILFPKNSFAASPLDKSPSKISNEQLKELNLTIENNKN
CCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHEEEEEECCCC
>Mature Secondary Structure
MFKIIKCNQINNKLINKKIEINGWVKKIRNHGKVTFINIRDRYDEAQILVSDEKLLKITS
CEEEEEECCCCHHHCCCEEEHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCHHHHHHHH
QIKMEYCIKIQGKLNLRPLELVNREMKTGELEIIAENIDIISKCNELPFMIENNNNASDN
HHCEEEEEEEECCCCCCHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCEEEECCCCCCCC
SKLEYRYLDLRREEQKQKIILRSKVIHIIRNHLTKQDFLELETPTFVKSTPEGARDFLVP
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHCCCC
SRIHKGHFYALPQSPQIYKQLAMIAGLDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFI
CCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHH
KKENIFKLIENLIFTIFKNSLNITLPKKFKKITYKDAMNIYGSDKPDTRYELLIQDMSKA
HHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LKQSPFDVFKDTLQNKGTIKALIIKNQAHNFSRSKINSLEEHAKLYKARTLYFTKIENNE
HHCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCC
FTGGIAKFINPIKKTLIETYSLKNNDLIFFIADLWETACKAIGQIRIKIATELNLINKNI
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHEEEEEEEHHHHHHHHH
FEFLWIYDFPLFEYDEDTQSYKAAHHMFSMPQAKYINTLESNPSEVLGEVYDLVLNGTEL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEE
GSGSIRVHTKELQQRIFNIVGFNDKIAEERFGFFLKALEYGAPIHGGIAIGIDRLLMLMT
CCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEE
NSNSIKDVILFPKNSFAASPLDKSPSKISNEQLKELNLTIENNKN
CCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA