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The map label for this gene is gyrB [H]

Identifier: 203284344

GI number: 203284344

Start: 466110

End: 468029

Strand: Reverse

Name: gyrB [H]

Synonym: BDU_433

Alternate gene names: 203284344

Gene position: 468029-466110 (Counterclockwise)

Preceding gene: 203284356

Following gene: 203284343

Centisome position: 50.24

GC content: 31.77

Gene sequence:

>1920_bases
TTGTGTGGAAGGTTTATGAGTTATGTTGCTAGTAATATTCAAGTTTTAAAGGGTCTTGAAGCTGTTAGAAAACGTCCTGG
TATGTATATTGGCTCTGTTTCAATTAATGGATTGCATCATTTAGTTTATGAAATTGTTGACAACAGTATTGATGAAGCCT
TGGCAGGATTTTGTGATAGGATAGAGGTTGTTATAAATTCAGATAATTCTATAATGGTGAATGATAATGGTAGGGGAATT
CCTACAGATATTCATGAAGAGGAAGGAATTAGTGCACTTGAACTTGTTTTGACAAAATTACATTCTGGAGGGAAATTTAA
TAAGGGTACTTATAAAGTTTCTGGTGGGCTTCATGGAGTTGGAGTTTCGGTTGTTAATGCTTTATCATCTTTCTTAGAAG
TAGTTGTTAGCAGAGAAGGAAAGCTTTTTAAACAAACGTATTCAAGAGGTATTCCGACGTCTGAGGTTGAAGTTGTTGGA
GTAAGTTCTACTACTGGTACGAAAGTTACTTTTTTTGCTGATTCTGAAATTTTTGAAACTTTAAAATATGATTTTGAGAC
TTTGTCAAGGAGGCTTAGAGAACTTGCATTTTTAAATGATAAAATACGGATTTCGATTGAAGATAGGAGATTAGGCGAAG
AAAAATTTTTAGAATTTTATTTTGAAGGTGGGATAAAAGCTTTTGTAGATTATATAACTAATAATAATAAAAATATTCAA
AGTAGTCCTTATTTTATTGAAGGAGTTTGTAATGATGTTATTGTTAGTGTTGGTCTTAAATGGACTGAAGGATATTCTGA
TAATACTTTATCTTTTGTAAATAATATTAATACTAAGGAGGGAGGAACTCATGTTGCTGGCTTTAAGAGTGGACTTTTAA
AAGCAATGAGTGAGGCTTTTAGAGATTCAAAAATAAGCAAAAAAGATATTCCCAATCTTACATTAGATGATTTTAAAGAA
GGACTAACAGCAGTAATTTCTATTAAAGTGCCAGAACCTCAGTTTGAGGGTCAAACCAAAGGAAAATTAGGAAATTCTTA
TATAAAAAAAATTGTTGAAACCATTGTATATGATGGACTTTTAAGGATTATTAATGAAAATTTTGTAGAAATAGATATTA
TTCTTAATAAGGCAATAAGAGCTGCTCGTGCTCGTGAAGCTGCAAGAAAAGCAAGGGAATCTGAGAGAAAGAAAAGTGCA
TTTGAAAGTTTGGCATTGCCTGGTAAATTAGCGGATTGTACATCTAAAAATCCAGCAGAGAGAGAAATCTACATTGTAGA
AGGTGATTCTGCAGGAGGAAGTGCAAAAATGGGAAGAGATAGGTTTTTTCAAGCTATTTTGCCATTATGGGGTAAAATGC
TTAATGTTGAGAAAACCAGAGAAGATAAAGTTATTACAAATGATAAGCTGATTCCAATAATTGCATCTCTTGGAGCTGGT
GTTGGTAAAGCTTTTTATATTGAAAAGCTTCGCTATCATAAAATTATTATTATGGCTGATGCTGATGTTGATGGATCTCA
TATTCGAACATTACTTCTTACATTTTTCTTTCGTTATATGAGAGAATTGATTGATAATAAGCATGTATATATAGCTATGC
CACCTCTTTATAAGATTAAACATGAAAATAAGGTGCATTATTTTTATGATGATTTTGAAAAGGAAACTTTTTTAGGGTCG
ATTGATGTTGTTAAAAGGAATAAAGTAAGTTTACAAAGATATAAAGGATTAGGAGAGATGAATCCTACACAACTTTGGGA
AACTACTATGAATCCTGCTACTAGAAAAATGAAGTTAATAGAAATAGATGATGCTATACAAGCTGAGAAAATTTTTGTTA
CTCTTATGGGGGATGATGTTGAACCTAGAAGAGAATTTATTGAGCAGAATGCACTTGATGTAGTAAATCTAGATGTGTGA

Upstream 100 bases:

>100_bases
ATTTTTAGGTCAAGCTAGATGTGTTTTGTAGTTTGTGTCATTTAATGGATATTATATTGTTTTTAATGTAGAATTTGCTT
ATGTCGTATATTATGATTAA

Downstream 100 bases:

>100_bases
TTGGAGCACTAATGGCAATTGAAAATAATAAAGAGCAAGTATTAAATATAAAGATAGAAGATGAGGTTAGGACTTCGTAT
TTAAATTATGCAATGTCTGT

Product: DNA gyrase, subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MCGRFMSYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEIVDNSIDEALAGFCDRIEVVINSDNSIMVNDNGRGI
PTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGVGVSVVNALSSFLEVVVSREGKLFKQTYSRGIPTSEVEVVG
VSSTTGTKVTFFADSEIFETLKYDFETLSRRLRELAFLNDKIRISIEDRRLGEEKFLEFYFEGGIKAFVDYITNNNKNIQ
SSPYFIEGVCNDVIVSVGLKWTEGYSDNTLSFVNNINTKEGGTHVAGFKSGLLKAMSEAFRDSKISKKDIPNLTLDDFKE
GLTAVISIKVPEPQFEGQTKGKLGNSYIKKIVETIVYDGLLRIINENFVEIDIILNKAIRAARAREAARKARESERKKSA
FESLALPGKLADCTSKNPAEREIYIVEGDSAGGSAKMGRDRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
VGKAFYIEKLRYHKIIIMADADVDGSHIRTLLLTFFFRYMRELIDNKHVYIAMPPLYKIKHENKVHYFYDDFEKETFLGS
IDVVKRNKVSLQRYKGLGEMNPTQLWETTMNPATRKMKLIEIDDAIQAEKIFVTLMGDDVEPRREFIEQNALDVVNLDV

Sequences:

>Translated_639_residues
MCGRFMSYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEIVDNSIDEALAGFCDRIEVVINSDNSIMVNDNGRGI
PTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGVGVSVVNALSSFLEVVVSREGKLFKQTYSRGIPTSEVEVVG
VSSTTGTKVTFFADSEIFETLKYDFETLSRRLRELAFLNDKIRISIEDRRLGEEKFLEFYFEGGIKAFVDYITNNNKNIQ
SSPYFIEGVCNDVIVSVGLKWTEGYSDNTLSFVNNINTKEGGTHVAGFKSGLLKAMSEAFRDSKISKKDIPNLTLDDFKE
GLTAVISIKVPEPQFEGQTKGKLGNSYIKKIVETIVYDGLLRIINENFVEIDIILNKAIRAARAREAARKARESERKKSA
FESLALPGKLADCTSKNPAEREIYIVEGDSAGGSAKMGRDRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
VGKAFYIEKLRYHKIIIMADADVDGSHIRTLLLTFFFRYMRELIDNKHVYIAMPPLYKIKHENKVHYFYDDFEKETFLGS
IDVVKRNKVSLQRYKGLGEMNPTQLWETTMNPATRKMKLIEIDDAIQAEKIFVTLMGDDVEPRREFIEQNALDVVNLDV
>Mature_639_residues
MCGRFMSYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEIVDNSIDEALAGFCDRIEVVINSDNSIMVNDNGRGI
PTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGVGVSVVNALSSFLEVVVSREGKLFKQTYSRGIPTSEVEVVG
VSSTTGTKVTFFADSEIFETLKYDFETLSRRLRELAFLNDKIRISIEDRRLGEEKFLEFYFEGGIKAFVDYITNNNKNIQ
SSPYFIEGVCNDVIVSVGLKWTEGYSDNTLSFVNNINTKEGGTHVAGFKSGLLKAMSEAFRDSKISKKDIPNLTLDDFKE
GLTAVISIKVPEPQFEGQTKGKLGNSYIKKIVETIVYDGLLRIINENFVEIDIILNKAIRAARAREAARKARESERKKSA
FESLALPGKLADCTSKNPAEREIYIVEGDSAGGSAKMGRDRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
VGKAFYIEKLRYHKIIIMADADVDGSHIRTLLLTFFFRYMRELIDNKHVYIAMPPLYKIKHENKVHYFYDDFEKETFLGS
IDVVKRNKVSLQRYKGLGEMNPTQLWETTMNPATRKMKLIEIDDAIQAEKIFVTLMGDDVEPRREFIEQNALDVVNLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=615, Percent_Identity=25.0406504065041, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI19913408, Length=619, Percent_Identity=24.5557350565428, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=51.7921146953405, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1789408, Length=601, Percent_Identity=40.432612312812, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17535065, Length=620, Percent_Identity=26.2903225806452, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=609, Percent_Identity=26.6009852216749, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=195, Percent_Identity=28.7179487179487, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=635, Percent_Identity=24.8818897637795, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17136538, Length=665, Percent_Identity=24.812030075188, Blast_Score=143, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71789; Mature: 71789

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGRFMSYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEIVDNSIDEALAGFCDR
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCHHHHHHHHHHCE
IEVVINSDNSIMVNDNGRGIPTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGV
EEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHH
GVSVVNALSSFLEVVVSREGKLFKQTYSRGIPTSEVEVVGVSSTTGTKVTFFADSEIFET
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECHHHHHH
LKYDFETLSRRLRELAFLNDKIRISIEDRRLGEEKFLEFYFEGGIKAFVDYITNNNKNIQ
HHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCCC
SSPYFIEGVCNDVIVSVGLKWTEGYSDNTLSFVNNINTKEGGTHVAGFKSGLLKAMSEAF
CCCCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHH
RDSKISKKDIPNLTLDDFKEGLTAVISIKVPEPQFEGQTKGKLGNSYIKKIVETIVYDGL
HCCCCCHHCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LRIINENFVEIDIILNKAIRAARAREAARKARESERKKSAFESLALPGKLADCTSKNPAE
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
REIYIVEGDSAGGSAKMGRDRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHCCC
VGKAFYIEKLRYHKIIIMADADVDGSHIRTLLLTFFFRYMRELIDNKHVYIAMPPLYKIK
CCHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEC
HENKVHYFYDDFEKETFLGSIDVVKRNKVSLQRYKGLGEMNPTQLWETTMNPATRKMKLI
CCCEEEEEECCCCCHHHCCCHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHEEEEE
EIDDAIQAEKIFVTLMGDDVEPRREFIEQNALDVVNLDV
EECCCCCEEEEEEEEECCCCCHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MCGRFMSYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEIVDNSIDEALAGFCDR
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCHHHHHHHHHHCE
IEVVINSDNSIMVNDNGRGIPTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGV
EEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHH
GVSVVNALSSFLEVVVSREGKLFKQTYSRGIPTSEVEVVGVSSTTGTKVTFFADSEIFET
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECHHHHHH
LKYDFETLSRRLRELAFLNDKIRISIEDRRLGEEKFLEFYFEGGIKAFVDYITNNNKNIQ
HHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCCC
SSPYFIEGVCNDVIVSVGLKWTEGYSDNTLSFVNNINTKEGGTHVAGFKSGLLKAMSEAF
CCCCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHH
RDSKISKKDIPNLTLDDFKEGLTAVISIKVPEPQFEGQTKGKLGNSYIKKIVETIVYDGL
HCCCCCHHCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LRIINENFVEIDIILNKAIRAARAREAARKARESERKKSAFESLALPGKLADCTSKNPAE
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
REIYIVEGDSAGGSAKMGRDRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHCCC
VGKAFYIEKLRYHKIIIMADADVDGSHIRTLLLTFFFRYMRELIDNKHVYIAMPPLYKIK
CCHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEC
HENKVHYFYDDFEKETFLGSIDVVKRNKVSLQRYKGLGEMNPTQLWETTMNPATRKMKLI
CCCEEEEEECCCCCHHHCCCHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHEEEEE
EIDDAIQAEKIFVTLMGDDVEPRREFIEQNALDVVNLDV
EECCCCCEEEEEEEEECCCCCHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA