Click here to switch to the map view.

The map label for this gene is gyrA [H]

Identifier: 203284343

GI number: 203284343

Start: 463666

End: 466113

Strand: Reverse

Name: gyrA [H]

Synonym: BDU_432

Alternate gene names: 203284343

Gene position: 466113-463666 (Counterclockwise)

Preceding gene: 203284344

Following gene: 203284342

Centisome position: 50.03

GC content: 31.9

Gene sequence:

>2448_bases
GTGATTGGAGCACTAATGGCAATTGAAAATAATAAAGAGCAAGTATTAAATATAAAGATAGAAGATGAGGTTAGGACTTC
GTATTTAAATTATGCAATGTCTGTTATTGTTTCAAGGGCTCTTCCTGATGTTAGAGATGGTCTTAAACCTGTTCACAGAA
GAATTTTATATTCAATGCATGAAATGGGTCTTAAGTCTGATAAGTCGTTTAAGAAGGCTGGGCGGATTGTTGGAGATGTT
CTTGGTAAGTATCATCCTCATGGTGATCAGTCAATTTATGAGGCTCTTGTAAGGCTTGCACAGGATTTTTCGTTAAGATA
TCCTATAGTTAGTGGACAGGGAAATTTTGGTTCTATTGATGGCGATCCTCCTGCTGCTATGCGATATACTGAAGCTCGAA
TGGCAAAAATAGCAGAAGAACTCGTTATAGATATAGATAAACAAACTGTTAATTTTAAGCCCAATTATGATGATTCTTTG
CTTGAACCTGAGGTTTTACCAGCAGCTTTTCCATTTCTTTTAGTTAATGGTTCTAGTGGTATTGCTGTTGGGATGGCTAC
AAATATGGCTCCTCATAATTTGAGAGAGGTTTGTGATGCCATAATTTATATGTTGGAGCATGATGATGTTTCTATTTACG
ATTTAATAGCAATAATTAAAGGTCCAGATTTTCCAACTTATGCTGAGATCATTTATAATGAAAATTTAATTAAGGCTTAT
ACTACGGGAAAAGGTAGCGTTGTTATTCGAGCACGTTATCATATTGAAGAAAAGTCGGAAGATAATATTTCTATTATAAT
TACACAAATACCTTATGCTGTTAACAAATCTTCTTTACTTATGAAGATTGCATTGTTAATAAAAGAAGAAAAATTGGAAG
GTGTATCTGATATAAGGGATGAGTCGGATCGTGAAGGTATTAGAATTGTTCTTGAAATAAAAAAAGGTTTTGATCCTCAT
GTTGTGATGAATTTACTTTATGAATATACCGAACTTAGGAGGAATTTTAGTATAAATAATTTAGCTCTTGTTAATGGTAT
TCCTAAACAATTAAATTTAAGAGAGCTTATATCTGAATTTATCGAGCATAGAAAGAATATTCTTAGACGGCGTGTAGAGT
TTGACTTAAAGAGAGCACAAGAGAAGGCCCATATTCTTGAAGGGTTGAATATTGCACTAAGAAATATTGATAGAGTAATA
GAAATAATACGATTTTCTAGACTTGTAAAAGATGCTAGAGAGTGTATTATGAGAGAATTTCAACTATCGGAAATACAGGC
TAATTCTATACTGGATATGAAGTTACAAAAGTTGACATCTCTTGAGGTGGCAAAAATAGAAGAAGATTTTCAAGTTGTAT
TAGCTTTAATAAAAGATTATGAAGATATTCTAATAAGTCCATCAAGAATTGTTAATATGATAAGAGAAGAGATTATTAAT
TTAAGTTTAAAATTTGGCGATGATCGCCGAACAAAAATAATTTATGATGAGGAGGTTTTAAAAACTAGTATGTCAGATTT
AATGCAGAGGGAAAATGTTATTGTTATTCTTACAAAGCAAGGTTTCATTAAAAGGGTCTTACAAGATGAATATAAACTTC
AAGGTATAGGTGGTAAGGGCCTTGGTTCTTTTGATTTACAAGATAGCGATCAAGTTAATGTTGCTTTATGTGTAAATACT
CATGACTTTTTATTGATGATTTCGAATGAAGGTAAGCTTTATGTAATTAATGCTTATGGGATTAAAGATTCTTCTAGAAC
TTCAAGAGGACAAAATATTCGTGAACTTATTAATTTAGGAGAAAAAGAAGAAATATTAGCTATTAAAAATTGCAGTGAAT
TGTCTGTAGATAATTATTTGTTAATAACAACCGCAAATGGAAAAATAGCACGTATTGAGACTGAAGGGTTTAAAACTGTT
AAATCAAGGGGCGTTATTATTATGAAACTTGATGATAAAGATTTTGTTACAAGTGCGGAGATTGTATCTAGAGATGATAA
AATTATTTGTATTTCCAAGAAAGGTAATGCTTTTGCATTTAGTTCTAATGACATTAGATTGACACATAGAGGAACTCAAG
GGGTGTCTGGAATGAAAGTAAGAGAGGGAGATATGCTTATTAAGGCTTTAGCCATAAAACAAGGATCTCATCTTTTAATT
ATGTCTGAGAATGGATATGGAAAGCGATTAGAAGTGTCTAAAGTAACTGGGCTTAAGAGAGGTTCTACTGGGTATACTTG
CTATAAGAAGTCTGATGAAAAGGCAGGTGAAGTTGTTGATGCTATTACAGTTTCTCAAGATGATGAAATATTGCTTATAA
GTAAAAGTTCAAAAGTGTTACGAACATTAGTTGACAAAATATCTGAACAAGGTAAGGATGCTAGAGGAATGCAGGTATTA
TCTCTTGATGAAGATAAATTAGTAGCTGTTTCAAAATTTATTAAATAA

Upstream 100 bases:

>100_bases
TACAAGCTGAGAAAATTTTTGTTACTCTTATGGGGGATGATGTTGAACCTAGAAGAGAATTTATTGAGCAGAATGCACTT
GATGTAGTAAATCTAGATGT

Downstream 100 bases:

>100_bases
TAAATCGATATTAATGATATTGTTAGAATGTTCCACGTGGAACATTCTTTTTTTTTGTGATTGTTTAAATTGAAAGTTAA
ATTAACATGGAACAACTTTA

Product: DNA gyrase, subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MIGALMAIENNKEQVLNIKIEDEVRTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMHEMGLKSDKSFKKAGRIVGDV
LGKYHPHGDQSIYEALVRLAQDFSLRYPIVSGQGNFGSIDGDPPAAMRYTEARMAKIAEELVIDIDKQTVNFKPNYDDSL
LEPEVLPAAFPFLLVNGSSGIAVGMATNMAPHNLREVCDAIIYMLEHDDVSIYDLIAIIKGPDFPTYAEIIYNENLIKAY
TTGKGSVVIRARYHIEEKSEDNISIIITQIPYAVNKSSLLMKIALLIKEEKLEGVSDIRDESDREGIRIVLEIKKGFDPH
VVMNLLYEYTELRRNFSINNLALVNGIPKQLNLRELISEFIEHRKNILRRRVEFDLKRAQEKAHILEGLNIALRNIDRVI
EIIRFSRLVKDARECIMREFQLSEIQANSILDMKLQKLTSLEVAKIEEDFQVVLALIKDYEDILISPSRIVNMIREEIIN
LSLKFGDDRRTKIIYDEEVLKTSMSDLMQRENVIVILTKQGFIKRVLQDEYKLQGIGGKGLGSFDLQDSDQVNVALCVNT
HDFLLMISNEGKLYVINAYGIKDSSRTSRGQNIRELINLGEKEEILAIKNCSELSVDNYLLITTANGKIARIETEGFKTV
KSRGVIIMKLDDKDFVTSAEIVSRDDKIICISKKGNAFAFSSNDIRLTHRGTQGVSGMKVREGDMLIKALAIKQGSHLLI
MSENGYGKRLEVSKVTGLKRGSTGYTCYKKSDEKAGEVVDAITVSQDDEILLISKSSKVLRTLVDKISEQGKDARGMQVL
SLDEDKLVAVSKFIK

Sequences:

>Translated_815_residues
MIGALMAIENNKEQVLNIKIEDEVRTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMHEMGLKSDKSFKKAGRIVGDV
LGKYHPHGDQSIYEALVRLAQDFSLRYPIVSGQGNFGSIDGDPPAAMRYTEARMAKIAEELVIDIDKQTVNFKPNYDDSL
LEPEVLPAAFPFLLVNGSSGIAVGMATNMAPHNLREVCDAIIYMLEHDDVSIYDLIAIIKGPDFPTYAEIIYNENLIKAY
TTGKGSVVIRARYHIEEKSEDNISIIITQIPYAVNKSSLLMKIALLIKEEKLEGVSDIRDESDREGIRIVLEIKKGFDPH
VVMNLLYEYTELRRNFSINNLALVNGIPKQLNLRELISEFIEHRKNILRRRVEFDLKRAQEKAHILEGLNIALRNIDRVI
EIIRFSRLVKDARECIMREFQLSEIQANSILDMKLQKLTSLEVAKIEEDFQVVLALIKDYEDILISPSRIVNMIREEIIN
LSLKFGDDRRTKIIYDEEVLKTSMSDLMQRENVIVILTKQGFIKRVLQDEYKLQGIGGKGLGSFDLQDSDQVNVALCVNT
HDFLLMISNEGKLYVINAYGIKDSSRTSRGQNIRELINLGEKEEILAIKNCSELSVDNYLLITTANGKIARIETEGFKTV
KSRGVIIMKLDDKDFVTSAEIVSRDDKIICISKKGNAFAFSSNDIRLTHRGTQGVSGMKVREGDMLIKALAIKQGSHLLI
MSENGYGKRLEVSKVTGLKRGSTGYTCYKKSDEKAGEVVDAITVSQDDEILLISKSSKVLRTLVDKISEQGKDARGMQVL
SLDEDKLVAVSKFIK
>Mature_815_residues
MIGALMAIENNKEQVLNIKIEDEVRTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMHEMGLKSDKSFKKAGRIVGDV
LGKYHPHGDQSIYEALVRLAQDFSLRYPIVSGQGNFGSIDGDPPAAMRYTEARMAKIAEELVIDIDKQTVNFKPNYDDSL
LEPEVLPAAFPFLLVNGSSGIAVGMATNMAPHNLREVCDAIIYMLEHDDVSIYDLIAIIKGPDFPTYAEIIYNENLIKAY
TTGKGSVVIRARYHIEEKSEDNISIIITQIPYAVNKSSLLMKIALLIKEEKLEGVSDIRDESDREGIRIVLEIKKGFDPH
VVMNLLYEYTELRRNFSINNLALVNGIPKQLNLRELISEFIEHRKNILRRRVEFDLKRAQEKAHILEGLNIALRNIDRVI
EIIRFSRLVKDARECIMREFQLSEIQANSILDMKLQKLTSLEVAKIEEDFQVVLALIKDYEDILISPSRIVNMIREEIIN
LSLKFGDDRRTKIIYDEEVLKTSMSDLMQRENVIVILTKQGFIKRVLQDEYKLQGIGGKGLGSFDLQDSDQVNVALCVNT
HDFLLMISNEGKLYVINAYGIKDSSRTSRGQNIRELINLGEKEEILAIKNCSELSVDNYLLITTANGKIARIETEGFKTV
KSRGVIIMKLDDKDFVTSAEIVSRDDKIICISKKGNAFAFSSNDIRLTHRGTQGVSGMKVREGDMLIKALAIKQGSHLLI
MSENGYGKRLEVSKVTGLKRGSTGYTCYKKSDEKAGEVVDAITVSQDDEILLISKSSKVLRTLVDKISEQGKDARGMQVL
SLDEDKLVAVSKFIK

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=179, Percent_Identity=30.7262569832402, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI19913406, Length=182, Percent_Identity=29.6703296703297, Blast_Score=76, Evalue=2e-13,
Organism=Escherichia coli, GI1788562, Length=830, Percent_Identity=40.9638554216867, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=745, Percent_Identity=34.8993288590604, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI212645845, Length=463, Percent_Identity=25.4859611231102, Blast_Score=96, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535065, Length=173, Percent_Identity=31.2138728323699, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI212645657, Length=185, Percent_Identity=29.1891891891892, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115534553, Length=161, Percent_Identity=29.1925465838509, Blast_Score=70, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=176, Percent_Identity=29.5454545454545, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136538, Length=180, Percent_Identity=27.2222222222222, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91910; Mature: 91910

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGALMAIENNKEQVLNIKIEDEVRTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMH
CCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EMGLKSDKSFKKAGRIVGDVLGKYHPHGDQSIYEALVRLAQDFSLRYPIVSGQGNFGSID
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCEEECCCCCCCCC
GDPPAAMRYTEARMAKIAEELVIDIDKQTVNFKPNYDDSLLEPEVLPAAFPFLLVNGSSG
CCCCHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCCCCHHHCCCCEEEEECCCC
IAVGMATNMAPHNLREVCDAIIYMLEHDDVSIYDLIAIIKGPDFPTYAEIIYNENLIKAY
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHEECCCEEEEE
TTGKGSVVIRARYHIEEKSEDNISIIITQIPYAVNKSSLLMKIALLIKEEKLEGVSDIRD
ECCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHCC
ESDREGIRIVLEIKKGFDPHVVMNLLYEYTELRRNFSINNLALVNGIPKQLNLRELISEF
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
IEHRKNILRRRVEFDLKRAQEKAHILEGLNIALRNIDRVIEIIRFSRLVKDARECIMREF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLSEIQANSILDMKLQKLTSLEVAKIEEDFQVVLALIKDYEDILISPSRIVNMIREEIIN
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHEECHHHHHHHHHHHHHE
LSLKFGDDRRTKIIYDEEVLKTSMSDLMQRENVIVILTKQGFIKRVLQDEYKLQGIGGKG
EEEEECCCCCEEEEECHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHEEEECCCCC
LGSFDLQDSDQVNVALCVNTHDFLLMISNEGKLYVINAYGIKDSSRTSRGQNIRELINLG
CCCCCCCCCCCEEEEEEEECCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCC
EKEEILAIKNCSELSVDNYLLITTANGKIARIETEGFKTVKSRGVIIMKLDDKDFVTSAE
CCCCEEEECCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHCCEEEEEECCCCHHCHHH
IVSRDDKIICISKKGNAFAFSSNDIRLTHRGTQGVSGMKVREGDMLIKALAIKQGSHLLI
HHCCCCCEEEEECCCCEEEEECCCEEEEECCCCCCCCCEEECCHHEEEEHEECCCCEEEE
MSENGYGKRLEVSKVTGLKRGSTGYTCYKKSDEKAGEVVDAITVSQDDEILLISKSSKVL
EECCCCCCEEEEHHHCCCCCCCCCEEEEECCCCCHHHHEEEEEECCCCCEEEEECCHHHH
RTLVDKISEQGKDARGMQVLSLDEDKLVAVSKFIK
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MIGALMAIENNKEQVLNIKIEDEVRTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMH
CCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EMGLKSDKSFKKAGRIVGDVLGKYHPHGDQSIYEALVRLAQDFSLRYPIVSGQGNFGSID
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCEEECCCCCCCCC
GDPPAAMRYTEARMAKIAEELVIDIDKQTVNFKPNYDDSLLEPEVLPAAFPFLLVNGSSG
CCCCHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCCCCHHHCCCCEEEEECCCC
IAVGMATNMAPHNLREVCDAIIYMLEHDDVSIYDLIAIIKGPDFPTYAEIIYNENLIKAY
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHEECCCEEEEE
TTGKGSVVIRARYHIEEKSEDNISIIITQIPYAVNKSSLLMKIALLIKEEKLEGVSDIRD
ECCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHCC
ESDREGIRIVLEIKKGFDPHVVMNLLYEYTELRRNFSINNLALVNGIPKQLNLRELISEF
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
IEHRKNILRRRVEFDLKRAQEKAHILEGLNIALRNIDRVIEIIRFSRLVKDARECIMREF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLSEIQANSILDMKLQKLTSLEVAKIEEDFQVVLALIKDYEDILISPSRIVNMIREEIIN
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHEECHHHHHHHHHHHHHE
LSLKFGDDRRTKIIYDEEVLKTSMSDLMQRENVIVILTKQGFIKRVLQDEYKLQGIGGKG
EEEEECCCCCEEEEECHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHEEEECCCCC
LGSFDLQDSDQVNVALCVNTHDFLLMISNEGKLYVINAYGIKDSSRTSRGQNIRELINLG
CCCCCCCCCCCEEEEEEEECCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCC
EKEEILAIKNCSELSVDNYLLITTANGKIARIETEGFKTVKSRGVIIMKLDDKDFVTSAE
CCCCEEEECCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHCCEEEEEECCCCHHCHHH
IVSRDDKIICISKKGNAFAFSSNDIRLTHRGTQGVSGMKVREGDMLIKALAIKQGSHLLI
HHCCCCCEEEEECCCCEEEEECCCEEEEECCCCCCCCCEEECCHHEEEEHEECCCCEEEE
MSENGYGKRLEVSKVTGLKRGSTGYTCYKKSDEKAGEVVDAITVSQDDEILLISKSSKVL
EECCCCCCEEEEHHHCCCCCCCCCEEEEECCCCCHHHHEEEEEECCCCCEEEEECCHHHH
RTLVDKISEQGKDARGMQVLSLDEDKLVAVSKFIK
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 1490605 [H]