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The map label for this gene is gatB

Identifier: 203284258

GI number: 203284258

Start: 362515

End: 363972

Strand: Reverse

Name: gatB

Synonym: BDU_340

Alternate gene names: 203284258

Gene position: 363972-362515 (Counterclockwise)

Preceding gene: 203284259

Following gene: 203284257

Centisome position: 39.07

GC content: 30.11

Gene sequence:

>1458_bases
ATGGAATATAAATTGCTTATTGGATTAGAAGTCCACGTACAATTAGGATTAAAGACTAAAGCTTTTTGTGGATGTAAAAA
TGATTTTGGTGGAATTCCAAATTCTCGTGTTTGTCCAATATGTCTTGGGCTTCCTGGAGCATTACCTAGCGTAAATAAAG
AACTTGTTAGTAGTGCAATTTTGGCAGGGCATGCTACTAATTCTACAATTAGAAATATTGTTAAATTCGATAGAAAGCAT
TATGCTTATCCTGATTTGCCCAAAGGATACCAAATATCTCAAAATGATGAGCCTATTTGTGAGAATGGGTTTATTTTTAT
TAAAACTAGTTTGGGTGTAAAAAGAATTAATATTGCAAGAATACATATGGAAGAGGATTCTGGAAAAAGTTTGCATTTGC
TTTCAAATGATAATCAAAGTTATATTGATTTTAATCGTGCAGGAGCACCATTGCTAGAAATTGTATCACAACCTGATATA
CGTAGTGGAGAAGAAGCCGTAGCTTATTTGAATGCTTTAAGGGAAATTTTTAGATATCTTGATTTGTCTGAGTGTAGTAT
GGAAAATGGATCATTTCGTTGTGATGTTAATATTAACTTACTTATTAATGAGAATGATGTTAACTATAAAACTCCCATTT
CTGAGATAAAAAATTTAAATTCTTTTAAGTCAGTAAAGTTAGCAATTGATTATGAGGAGTCAAAACAAAAAGAAGAATGG
ATTTTGTATAGAAAAACTTTGGAAAGTGTCGGGAAGTGTACAATGGGATTTGATGATAAGAAAGGTATTACAGTTCTTCA
AAGAAGCAAAGAAACAATATCTGATTATCGTTATATCAAGGATCCAGATTTACCTTTAATTAAACTTGAGAGTGATTATA
TTGAAAATATTAAATCTCATAGGATGGTGGAGTTACCTTTTGATGCAAGAATTAGGTTGCAAGAGCAGTATGGGCTTAGT
GATTTTGATGTTGTAACTTTAACTTCAGATAAAAATTTGGTTAAATATTTTGAAGAGGCAGCATTAACTTCAAGTGAACC
TAAGAAAGTGGCTAATTGGATATTATCTGAAGTGTTAAGTGTGTTAAATGAAAGGGAAATAGGTATACTTGATTTTAATT
TACCAGCATCATATATTGGTGAGCTTGTTGAATTTATTCTTAATGGAAAAATAAGTGGAAAGATTTCTAAGGAAATATTT
TTAGAAATGGTTGACAGAAATGTTTCTTCTATTACTATTATCAATGAAAAAAAATTAGAACAGATAAGCGATAAATCGTT
TATTGAATCAATTGTAATTGAAGTGTTAAACGAAAATCCTAAATCAATTGAACTTTACAAAAAAGGGAAAAGTCATGCTG
TTAAATTTATGATGGGACAGATTATGCGTAAAACTTCTGGTAAGGTTAATCCTGTTCTTTCAAATGAAATTTTAATGAAT
AAATTGCAGGATGTGTAG

Upstream 100 bases:

>100_bases
GATAAGGGATTACCTATTGGTATGCAAGTTATTGGTAGAGTTAAGAGGGATTTTGAACTTTTAAGTTTTTCAAAAAATAT
AATAGAGGAATTAGAATTAG

Downstream 100 bases:

>100_bases
TAAATCTTTATTGAGTTGTTTTCCCATAATTAAAAGAGCAAGATTTTTTTATCTTTATGATATTAATGGTAATCGGTATT
TAGATTTATATTTAAATGAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MEYKLLIGLEVHVQLGLKTKAFCGCKNDFGGIPNSRVCPICLGLPGALPSVNKELVSSAILAGHATNSTIRNIVKFDRKH
YAYPDLPKGYQISQNDEPICENGFIFIKTSLGVKRINIARIHMEEDSGKSLHLLSNDNQSYIDFNRAGAPLLEIVSQPDI
RSGEEAVAYLNALREIFRYLDLSECSMENGSFRCDVNINLLINENDVNYKTPISEIKNLNSFKSVKLAIDYEESKQKEEW
ILYRKTLESVGKCTMGFDDKKGITVLQRSKETISDYRYIKDPDLPLIKLESDYIENIKSHRMVELPFDARIRLQEQYGLS
DFDVVTLTSDKNLVKYFEEAALTSSEPKKVANWILSEVLSVLNEREIGILDFNLPASYIGELVEFILNGKISGKISKEIF
LEMVDRNVSSITIINEKKLEQISDKSFIESIVIEVLNENPKSIELYKKGKSHAVKFMMGQIMRKTSGKVNPVLSNEILMN
KLQDV

Sequences:

>Translated_485_residues
MEYKLLIGLEVHVQLGLKTKAFCGCKNDFGGIPNSRVCPICLGLPGALPSVNKELVSSAILAGHATNSTIRNIVKFDRKH
YAYPDLPKGYQISQNDEPICENGFIFIKTSLGVKRINIARIHMEEDSGKSLHLLSNDNQSYIDFNRAGAPLLEIVSQPDI
RSGEEAVAYLNALREIFRYLDLSECSMENGSFRCDVNINLLINENDVNYKTPISEIKNLNSFKSVKLAIDYEESKQKEEW
ILYRKTLESVGKCTMGFDDKKGITVLQRSKETISDYRYIKDPDLPLIKLESDYIENIKSHRMVELPFDARIRLQEQYGLS
DFDVVTLTSDKNLVKYFEEAALTSSEPKKVANWILSEVLSVLNEREIGILDFNLPASYIGELVEFILNGKISGKISKEIF
LEMVDRNVSSITIINEKKLEQISDKSFIESIVIEVLNENPKSIELYKKGKSHAVKFMMGQIMRKTSGKVNPVLSNEILMN
KLQDV
>Mature_485_residues
MEYKLLIGLEVHVQLGLKTKAFCGCKNDFGGIPNSRVCPICLGLPGALPSVNKELVSSAILAGHATNSTIRNIVKFDRKH
YAYPDLPKGYQISQNDEPICENGFIFIKTSLGVKRINIARIHMEEDSGKSLHLLSNDNQSYIDFNRAGAPLLEIVSQPDI
RSGEEAVAYLNALREIFRYLDLSECSMENGSFRCDVNINLLINENDVNYKTPISEIKNLNSFKSVKLAIDYEESKQKEEW
ILYRKTLESVGKCTMGFDDKKGITVLQRSKETISDYRYIKDPDLPLIKLESDYIENIKSHRMVELPFDARIRLQEQYGLS
DFDVVTLTSDKNLVKYFEEAALTSSEPKKVANWILSEVLSVLNEREIGILDFNLPASYIGELVEFILNGKISGKISKEIF
LEMVDRNVSSITIINEKKLEQISDKSFIESIVIEVLNENPKSIELYKKGKSHAVKFMMGQIMRKTSGKVNPVLSNEILMN
KLQDV

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=508, Percent_Identity=33.6614173228346, Blast_Score=254, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI115534094, Length=343, Percent_Identity=33.8192419825073, Blast_Score=178, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6319391, Length=528, Percent_Identity=30.8712121212121, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=30.7535641547862, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_BORDL (B5RLS6)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221998.1
- EnsemblBacteria:   EBBORT00000000983
- GeneID:   6918424
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_340
- GeneTree:   EBGT00050000007362
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: NA

Molecular weight: Translated: 54930; Mature: 54930

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKLLIGLEVHVQLGLKTKAFCGCKNDFGGIPNSRVCPICLGLPGALPSVNKELVSSAI
CCEEEEEEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
LAGHATNSTIRNIVKFDRKHYAYPDLPKGYQISQNDEPICENGFIFIKTSLGVKRINIAR
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEE
IHMEEDSGKSLHLLSNDNQSYIDFNRAGAPLLEIVSQPDIRSGEEAVAYLNALREIFRYL
EEEECCCCCEEEEEECCCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
DLSECSMENGSFRCDVNINLLINENDVNYKTPISEIKNLNSFKSVKLAIDYEESKQKEEW
CHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHCCCCCCEEEEEEECHHHCCHHHH
ILYRKTLESVGKCTMGFDDKKGITVLQRSKETISDYRYIKDPDLPLIKLESDYIENIKSH
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHC
RMVELPFDARIRLQEQYGLSDFDVVTLTSDKNLVKYFEEAALTSSEPKKVANWILSEVLS
CEEECCCCCEEEEHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VLNEREIGILDFNLPASYIGELVEFILNGKISGKISKEIFLEMVDRNVSSITIINEKKLE
HHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHH
QISDKSFIESIVIEVLNENPKSIELYKKGKSHAVKFMMGQIMRKTSGKVNPVLSNEILMN
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KLQDV
HHHCC
>Mature Secondary Structure
MEYKLLIGLEVHVQLGLKTKAFCGCKNDFGGIPNSRVCPICLGLPGALPSVNKELVSSAI
CCEEEEEEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
LAGHATNSTIRNIVKFDRKHYAYPDLPKGYQISQNDEPICENGFIFIKTSLGVKRINIAR
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEE
IHMEEDSGKSLHLLSNDNQSYIDFNRAGAPLLEIVSQPDIRSGEEAVAYLNALREIFRYL
EEEECCCCCEEEEEECCCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
DLSECSMENGSFRCDVNINLLINENDVNYKTPISEIKNLNSFKSVKLAIDYEESKQKEEW
CHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHCCCCCCEEEEEEECHHHCCHHHH
ILYRKTLESVGKCTMGFDDKKGITVLQRSKETISDYRYIKDPDLPLIKLESDYIENIKSH
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHC
RMVELPFDARIRLQEQYGLSDFDVVTLTSDKNLVKYFEEAALTSSEPKKVANWILSEVLS
CEEECCCCCEEEEHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VLNEREIGILDFNLPASYIGELVEFILNGKISGKISKEIFLEMVDRNVSSITIINEKKLE
HHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHH
QISDKSFIESIVIEVLNENPKSIELYKKGKSHAVKFMMGQIMRKTSGKVNPVLSNEILMN
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KLQDV
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA