Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 203284259

GI number: 203284259

Start: 363962

End: 365407

Strand: Reverse

Name: gatA [H]

Synonym: BDU_341

Alternate gene names: 203284259

Gene position: 365407-363962 (Counterclockwise)

Preceding gene: 203284260

Following gene: 203284258

Centisome position: 39.22

GC content: 29.53

Gene sequence:

>1446_bases
TTGGACTTAAGTGGTTTGAATTTAATAAAAATTAAAGAATTAATATTGACTCGGAAATATAAGATTTATGATATTATCCT
TTATTATAAGAAAGTTTATGAAGATAATAGAGATATTAATGGTTATGTTGAGTTTTTTGATGATGCATTGGAAATAGCCA
AAGAATATGATGATCTTTTAAGTAAAGGTGGGGGACAAGATTTGCCTTTACTTGGTATTCCTATTGCCGTTAAGGATAAT
ATTGCAATTAAAAATAAAGATTTAACTTGTGCATCTGAGATTTTGCAAGGTTATATTTCTCCTTATGATGCAACTGTTAT
TAAAAGATTAAAAGATAATGGGGCAATTATAATTGGAAGAACTAATATGGATGAGTTTGCAATGGGTTCTTCTTGTGAAT
TTTCTTATTATGGAGTTACTCTTAATCCTTCAAATAAAGAATATGTTGTGGGGGGAAGTTCTGGTGGTTCTGCAGCTGTT
GTTGCTGGAGGTCAAGCCCCTTTTTCACTTGGAAGTGATACGGGTGGTTCTGTTAGGCTTCCTGCTTCATTTGCAGGTGT
AATTGGATTTAAGCCTTCCTATGGAGGATTGTCTCGCTATGGACTGTCATCTTATTCTTCGTCTCTTGATCAAATAGGAT
TTTTTGCTAATTCTATTGATGACGTGGCTTTAATATTAAAATATACTTGTGGAATTGATACAATGGATTCTACTAGTATA
GATATTATTCAAGAACCCTATCCATTATTAAATAAACCTTTAAAAGGTACTAAATTGGCTGTTATTAAAGAGTTTAGTAA
AGACTTGATGGAAGAGGATATTTATTGTGAGTTTTCTAAATTTAAGTCTGCTCTTTTAAGTAAGGGTGTTGAGATACATG
AAATTTCAATAGAAGCATTTAATTTTGTACTTTCTCTTTATTATTCAATATCTCCTGTTGAAGCTGCATCTAATCTTGCT
CGTTATACTTGTCTTCATTATGGAAAAAGATTAAATGATACATTGAGTCTTAATGATTTTTATTACAAACATAGAAGTTT
ATTTTTACAAGAAGAAGTTAAAAGGCGTGTTGTACTTGGTAATTATTTATTATCAGAAGGATATGATTTGGAATATTATA
CTAAGGCTTGTAAAATTATAGAAAACGTATTGATTCCAAAATTTGATGAAATTTTTAACAATTATTCGTATATTATAACT
CCCACAAGTTTTTTGAAACCTTTTAAAATAGGTGAAAATTTTGATGATCCTTTGAAAATGTATTATTCTGATTTATGTAC
TGTGATTGCCAATCTTATTGGTTTTCCTGCACTTTCAATTCCATTTGCTAAAGATGATAAGGGATTACCTATTGGTATGC
AAGTTATTGGTAGAGTTAAGAGGGATTTTGAACTTTTAAGTTTTTCAAAAAATATAATAGAGGAATTAGAATTAGATGGA
ATATAA

Upstream 100 bases:

>100_bases
TTTTGCCTTCGTTGACAATAGAATCTATTAAGAATTTTAGCAATGTTTTTGTTGATGGTTATTTTTTATCACCTAAAGTA
CTTGAATAGGGAGTAATGAT

Downstream 100 bases:

>100_bases
ATTGCTTATTGGATTAGAAGTCCACGTACAATTAGGATTAAAGACTAAAGCTTTTTGTGGATGTAAAAATGATTTTGGTG
GAATTCCAAATTCTCGTGTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MDLSGLNLIKIKELILTRKYKIYDIILYYKKVYEDNRDINGYVEFFDDALEIAKEYDDLLSKGGGQDLPLLGIPIAVKDN
IAIKNKDLTCASEILQGYISPYDATVIKRLKDNGAIIIGRTNMDEFAMGSSCEFSYYGVTLNPSNKEYVVGGSSGGSAAV
VAGGQAPFSLGSDTGGSVRLPASFAGVIGFKPSYGGLSRYGLSSYSSSLDQIGFFANSIDDVALILKYTCGIDTMDSTSI
DIIQEPYPLLNKPLKGTKLAVIKEFSKDLMEEDIYCEFSKFKSALLSKGVEIHEISIEAFNFVLSLYYSISPVEAASNLA
RYTCLHYGKRLNDTLSLNDFYYKHRSLFLQEEVKRRVVLGNYLLSEGYDLEYYTKACKIIENVLIPKFDEIFNNYSYIIT
PTSFLKPFKIGENFDDPLKMYYSDLCTVIANLIGFPALSIPFAKDDKGLPIGMQVIGRVKRDFELLSFSKNIIEELELDG
I

Sequences:

>Translated_481_residues
MDLSGLNLIKIKELILTRKYKIYDIILYYKKVYEDNRDINGYVEFFDDALEIAKEYDDLLSKGGGQDLPLLGIPIAVKDN
IAIKNKDLTCASEILQGYISPYDATVIKRLKDNGAIIIGRTNMDEFAMGSSCEFSYYGVTLNPSNKEYVVGGSSGGSAAV
VAGGQAPFSLGSDTGGSVRLPASFAGVIGFKPSYGGLSRYGLSSYSSSLDQIGFFANSIDDVALILKYTCGIDTMDSTSI
DIIQEPYPLLNKPLKGTKLAVIKEFSKDLMEEDIYCEFSKFKSALLSKGVEIHEISIEAFNFVLSLYYSISPVEAASNLA
RYTCLHYGKRLNDTLSLNDFYYKHRSLFLQEEVKRRVVLGNYLLSEGYDLEYYTKACKIIENVLIPKFDEIFNNYSYIIT
PTSFLKPFKIGENFDDPLKMYYSDLCTVIANLIGFPALSIPFAKDDKGLPIGMQVIGRVKRDFELLSFSKNIIEELELDG
I
>Mature_481_residues
MDLSGLNLIKIKELILTRKYKIYDIILYYKKVYEDNRDINGYVEFFDDALEIAKEYDDLLSKGGGQDLPLLGIPIAVKDN
IAIKNKDLTCASEILQGYISPYDATVIKRLKDNGAIIIGRTNMDEFAMGSSCEFSYYGVTLNPSNKEYVVGGSSGGSAAV
VAGGQAPFSLGSDTGGSVRLPASFAGVIGFKPSYGGLSRYGLSSYSSSLDQIGFFANSIDDVALILKYTCGIDTMDSTSI
DIIQEPYPLLNKPLKGTKLAVIKEFSKDLMEEDIYCEFSKFKSALLSKGVEIHEISIEAFNFVLSLYYSISPVEAASNLA
RYTCLHYGKRLNDTLSLNDFYYKHRSLFLQEEVKRRVVLGNYLLSEGYDLEYYTKACKIIENVLIPKFDEIFNNYSYIIT
PTSFLKPFKIGENFDDPLKMYYSDLCTVIANLIGFPALSIPFAKDDKGLPIGMQVIGRVKRDFELLSFSKNIIEELELDG
I

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=466, Percent_Identity=36.0515021459227, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI195972892, Length=495, Percent_Identity=25.4545454545455, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI166795287, Length=178, Percent_Identity=32.5842696629214, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=421, Percent_Identity=36.104513064133, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17537465, Length=472, Percent_Identity=24.5762711864407, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=168, Percent_Identity=38.0952380952381, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=278, Percent_Identity=28.4172661870504, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=171, Percent_Identity=30.9941520467836, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=205, Percent_Identity=27.8048780487805, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=233, Percent_Identity=30.9012875536481, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=233, Percent_Identity=30.9012875536481, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=38.2830626450116, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=436, Percent_Identity=29.8165137614679, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=153, Percent_Identity=31.3725490196078, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=35.2668213457077, Blast_Score=231, Evalue=9e-61,
Organism=Drosophila melanogaster, GI45550774, Length=500, Percent_Identity=25.4, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24644968, Length=464, Percent_Identity=25.2155172413793, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648435, Length=500, Percent_Identity=25.4, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24648437, Length=500, Percent_Identity=25.4, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21356731, Length=202, Percent_Identity=34.1584158415842, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24652985, Length=471, Percent_Identity=25.0530785562633, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922090, Length=471, Percent_Identity=25.0530785562633, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652981, Length=471, Percent_Identity=25.0530785562633, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652983, Length=471, Percent_Identity=25.0530785562633, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078093, Length=490, Percent_Identity=23.6734693877551, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648441, Length=432, Percent_Identity=24.7685185185185, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=432, Percent_Identity=24.7685185185185, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 53644; Mature: 53644

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSGLNLIKIKELILTRKYKIYDIILYYKKVYEDNRDINGYVEFFDDALEIAKEYDDLL
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
SKGGGQDLPLLGIPIAVKDNIAIKNKDLTCASEILQGYISPYDATVIKRLKDNGAIIIGR
HCCCCCCCCEEECCEEEECCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEE
TNMDEFAMGSSCEFSYYGVTLNPSNKEYVVGGSSGGSAAVVAGGQAPFSLGSDTGGSVRL
CCCHHHCCCCCCCEEEEEEEECCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEC
PASFAGVIGFKPSYGGLSRYGLSSYSSSLDQIGFFANSIDDVALILKYTCGIDTMDSTSI
CHHHHHHEECCCCCCCCHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
DIIQEPYPLLNKPLKGTKLAVIKEFSKDLMEEDIYCEFSKFKSALLSKGVEIHEISIEAF
HHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHCCCEEEEEHHHHH
NFVLSLYYSISPVEAASNLARYTCLHYGKRLNDTLSLNDFYYKHRSLFLQEEVKRRVVLG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
NYLLSEGYDLEYYTKACKIIENVLIPKFDEIFNNYSYIITPTSFLKPFKIGENFDDPLKM
HHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEECHHHCCCCCCCCCCCHHHHH
YYSDLCTVIANLIGFPALSIPFAKDDKGLPIGMQVIGRVKRDFELLSFSKNIIEELELDG
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
I
C
>Mature Secondary Structure
MDLSGLNLIKIKELILTRKYKIYDIILYYKKVYEDNRDINGYVEFFDDALEIAKEYDDLL
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
SKGGGQDLPLLGIPIAVKDNIAIKNKDLTCASEILQGYISPYDATVIKRLKDNGAIIIGR
HCCCCCCCCEEECCEEEECCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEE
TNMDEFAMGSSCEFSYYGVTLNPSNKEYVVGGSSGGSAAVVAGGQAPFSLGSDTGGSVRL
CCCHHHCCCCCCCEEEEEEEECCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEC
PASFAGVIGFKPSYGGLSRYGLSSYSSSLDQIGFFANSIDDVALILKYTCGIDTMDSTSI
CHHHHHHEECCCCCCCCHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
DIIQEPYPLLNKPLKGTKLAVIKEFSKDLMEEDIYCEFSKFKSALLSKGVEIHEISIEAF
HHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHCCCEEEEEHHHHH
NFVLSLYYSISPVEAASNLARYTCLHYGKRLNDTLSLNDFYYKHRSLFLQEEVKRRVVLG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
NYLLSEGYDLEYYTKACKIIENVLIPKFDEIFNNYSYIITPTSFLKPFKIGENFDDPLKM
HHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEECHHHCCCCCCCCCCCHHHHH
YYSDLCTVIANLIGFPALSIPFAKDDKGLPIGMQVIGRVKRDFELLSFSKNIIEELELDG
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]