Click here to switch to the map view.

The map label for this gene is proW [H]

Identifier: 203284070

GI number: 203284070

Start: 157130

End: 158029

Strand: Reverse

Name: proW [H]

Synonym: BDU_148

Alternate gene names: 203284070

Gene position: 158029-157130 (Counterclockwise)

Preceding gene: 203284071

Following gene: 203284069

Centisome position: 16.96

GC content: 29.11

Gene sequence:

>900_bases
ATGAATAGGGATTTTATAGTTAGTAGTATAGATAAGGCATTTGATTTTTTGGTTGATAATTTTGCAAATTCTAGTGGTAT
TGGATTTGCTAAGGTTATAGATTTTTTTTATGAAAATTTGAAAAGGTTGTTTACCGTAATTAATCCTGTTCTTTTTATTG
TAATAATTTGTGTTTTTAGTTTTTTATTTTTGAAAAAAAGATTGGCACTTTTGATTATGTTGGGCTTTTGTTTTATTTTA
TATTTTGATCTTTGGGAAGTTTCTATGGATACAATATCACTTATTTTTGTATCTGTATTTTTTTCTGTACTTTGGGGAAT
TTTAATAGGTATTTTGGGTGGGTATTGTGCAAAATTTTATGTTTTTTTAAAACCATTTCTTGATTTGATGCAAGCAATGC
CCCCATTTGTTTATTTGATCCCAGCTATATCGTTTTTCGATACGGGTACGTCTTCGGCAATTTTTGCTACAATAATTTTT
GCAATGCCCCCAGTTATTAGATATACACGATTAGGAATTATTCAAGTTCCAAGTGAGGTTATTGAAGCTGCAAAATCTTT
TGGAAGTAGTAGTGCTCGTATTCTTTTTCAAATTCAATTACCATTGGCTTTACAGAGTATAATAGAGGGGATTAATCAAT
CAATAATGATGGCAATATCTATGATAGTGATTGCGGCAATGGTTGGGTCCTCAGGACTTGGTAGAACCGTGATATATTCT
GTTGAAAGGTTACATTTTGCTGAAGGTTTAATATCTGGATTGGCTGTTGTAATAATAGCTATTATTTTAGATAGAATTAT
GCAAGCTATTTTCATTAAATTTAGTTATTTAAATACTGATAATTATGGGGGTAAAAAAGAAAATAAGTTCAAAAGGTTTT
TAGAAATGTATAACAAATAA

Upstream 100 bases:

>100_bases
AAATAAGTTAGATTATTTGATTGTGACAAAAGAACAAGGTGATATTATTGGATATATTGATTTAGGAGAGATTGCTGCTT
TATTGGCAAGATAGTTATTT

Downstream 100 bases:

>100_bases
TGCTTTGTTTGTATTTGTTAGAAGGGGGATTGGTTTATGAAAGGTATTTTTATACCTGTTGTTGTAAGTTTTGTTTTAAT
TTTTGTGGCTTGCAGTAAAG

Product: glycine betaine/L-proline transport system permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNRDFIVSSIDKAFDFLVDNFANSSGIGFAKVIDFFYENLKRLFTVINPVLFIVIICVFSFLFLKKRLALLIMLGFCFIL
YFDLWEVSMDTISLIFVSVFFSVLWGILIGILGGYCAKFYVFLKPFLDLMQAMPPFVYLIPAISFFDTGTSSAIFATIIF
AMPPVIRYTRLGIIQVPSEVIEAAKSFGSSSARILFQIQLPLALQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
VERLHFAEGLISGLAVVIIAIILDRIMQAIFIKFSYLNTDNYGGKKENKFKRFLEMYNK

Sequences:

>Translated_299_residues
MNRDFIVSSIDKAFDFLVDNFANSSGIGFAKVIDFFYENLKRLFTVINPVLFIVIICVFSFLFLKKRLALLIMLGFCFIL
YFDLWEVSMDTISLIFVSVFFSVLWGILIGILGGYCAKFYVFLKPFLDLMQAMPPFVYLIPAISFFDTGTSSAIFATIIF
AMPPVIRYTRLGIIQVPSEVIEAAKSFGSSSARILFQIQLPLALQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
VERLHFAEGLISGLAVVIIAIILDRIMQAIFIKFSYLNTDNYGGKKENKFKRFLEMYNK
>Mature_299_residues
MNRDFIVSSIDKAFDFLVDNFANSSGIGFAKVIDFFYENLKRLFTVINPVLFIVIICVFSFLFLKKRLALLIMLGFCFIL
YFDLWEVSMDTISLIFVSVFFSVLWGILIGILGGYCAKFYVFLKPFLDLMQAMPPFVYLIPAISFFDTGTSSAIFATIIF
AMPPVIRYTRLGIIQVPSEVIEAAKSFGSSSARILFQIQLPLALQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
VERLHFAEGLISGLAVVIIAIILDRIMQAIFIKFSYLNTDNYGGKKENKFKRFLEMYNK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=263, Percent_Identity=33.8403041825095, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1788449, Length=177, Percent_Identity=29.9435028248588, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788451, Length=150, Percent_Identity=27.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786564, Length=153, Percent_Identity=30.0653594771242, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33657; Mature: 33657

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRDFIVSSIDKAFDFLVDNFANSSGIGFAKVIDFFYENLKRLFTVINPVLFIVIICVFS
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLFLKKRLALLIMLGFCFILYFDLWEVSMDTISLIFVSVFFSVLWGILIGILGGYCAKFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFLKPFLDLMQAMPPFVYLIPAISFFDTGTSSAIFATIIFAMPPVIRYTRLGIIQVPSEV
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHCCHHHCCHHH
IEAAKSFGSSSARILFQIQLPLALQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
HHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VERLHFAEGLISGLAVVIIAIILDRIMQAIFIKFSYLNTDNYGGKKENKFKRFLEMYNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNRDFIVSSIDKAFDFLVDNFANSSGIGFAKVIDFFYENLKRLFTVINPVLFIVIICVFS
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLFLKKRLALLIMLGFCFILYFDLWEVSMDTISLIFVSVFFSVLWGILIGILGGYCAKFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFLKPFLDLMQAMPPFVYLIPAISFFDTGTSSAIFATIIFAMPPVIRYTRLGIIQVPSEV
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHCCHHHCCHHH
IEAAKSFGSSSARILFQIQLPLALQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
HHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VERLHFAEGLISGLAVVIIAIILDRIMQAIFIKFSYLNTDNYGGKKENKFKRFLEMYNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]