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The map label for this gene is murI [H]

Identifier: 203284025

GI number: 203284025

Start: 108744

End: 109508

Strand: Reverse

Name: murI [H]

Synonym: BDU_103

Alternate gene names: 203284025

Gene position: 109508-108744 (Counterclockwise)

Preceding gene: 203284028

Following gene: 203284024

Centisome position: 11.75

GC content: 23.4

Gene sequence:

>765_bases
ATGAACAATTTAGAAGATGTTATAGTTATTTTTGATTCAGGTATTGGTGGACTTGCTTATTTTGAATATATAAGTAAGAA
ATTGTTTAATAGAAATTATATTTATGTTGCAGATAATAAAAATTTTCCTTATGGTGATAAAAGTTCAAATTTTATTTTAA
ATGAGTTTTTGGAGCTTATTTCAAAATTAAGGGAAATATATAATATTGTCTCTATTGTTGTTGCGTGTAATACAGCTTCT
ATTAGTGTTTATAATAAATTAAATTTTAATTTTCCTATAATATATACTTTGCCTTCAGTTAGTTTAGTTAAGGAACTTTT
GTGTAAAAGGGTGATTTTAATTGCAACAAATACTACTATTAATAGCGAATTTGTCAAGAAAGAGCAGAATAATCATATAG
ATTTGATTTTAAAATCTGCGGGTGAACTTGTAAAATTTGTTGAATATGGAGATATCTTTGAAAAAGATGCGCTTGAGTAT
TTAAAATCTTTAAAGTTTGAAGTTCAATCTACTAAACGAGATATGGTTTTTTTGGGTTGTACTCATTATTTGCATATTAA
AGATATGATAGAAAGTGTTTTAGAAATACCCGTTTATGATAATCGTAAAATTGTTGCCGATGAACTTAATAAATCGTTAA
AAAATGTTGAAAATAGTGATTATTGTTTTAAACGTTATTTTTATTTAACACAAGATAAAAATTTACACTTTTATAAGAAT
TTTTGTGAAAAATATAATTTTTATTTTAAAGGAATATTCAATTGA

Upstream 100 bases:

>100_bases
CTTGATGTTTATTATAATTATAGTTTAAGCCATATTATTTTATCATTTTGTCATTGAAAGAGAGTAAATTAATTTTTATA
CTTAAATTTAGAGAAGATTT

Downstream 100 bases:

>100_bases
ATAGTAGTACATTTGAGGTTCTCTGGGGTGTTAATAATATTTATTCTGTTGTTGAAGTTAATACTAATACAGTTTATGAG
GGTGTTATTAAAGGTAAATT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNNLEDVIVIFDSGIGGLAYFEYISKKLFNRNYIYVADNKNFPYGDKSSNFILNEFLELISKLREIYNIVSIVVACNTAS
ISVYNKLNFNFPIIYTLPSVSLVKELLCKRVILIATNTTINSEFVKKEQNNHIDLILKSAGELVKFVEYGDIFEKDALEY
LKSLKFEVQSTKRDMVFLGCTHYLHIKDMIESVLEIPVYDNRKIVADELNKSLKNVENSDYCFKRYFYLTQDKNLHFYKN
FCEKYNFYFKGIFN

Sequences:

>Translated_254_residues
MNNLEDVIVIFDSGIGGLAYFEYISKKLFNRNYIYVADNKNFPYGDKSSNFILNEFLELISKLREIYNIVSIVVACNTAS
ISVYNKLNFNFPIIYTLPSVSLVKELLCKRVILIATNTTINSEFVKKEQNNHIDLILKSAGELVKFVEYGDIFEKDALEY
LKSLKFEVQSTKRDMVFLGCTHYLHIKDMIESVLEIPVYDNRKIVADELNKSLKNVENSDYCFKRYFYLTQDKNLHFYKN
FCEKYNFYFKGIFN
>Mature_254_residues
MNNLEDVIVIFDSGIGGLAYFEYISKKLFNRNYIYVADNKNFPYGDKSSNFILNEFLELISKLREIYNIVSIVVACNTAS
ISVYNKLNFNFPIIYTLPSVSLVKELLCKRVILIATNTTINSEFVKKEQNNHIDLILKSAGELVKFVEYGDIFEKDALEY
LKSLKFEVQSTKRDMVFLGCTHYLHIKDMIESVLEIPVYDNRKIVADELNKSLKNVENSDYCFKRYFYLTQDKNLHFYKN
FCEKYNFYFKGIFN

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=186, Percent_Identity=32.7956989247312, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 29800; Mature: 29800

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00923 ASP_GLU_RACEMASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLEDVIVIFDSGIGGLAYFEYISKKLFNRNYIYVADNKNFPYGDKSSNFILNEFLELI
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
SKLREIYNIVSIVVACNTASISVYNKLNFNFPIIYTLPSVSLVKELLCKRVILIATNTTI
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHCEEEEEEECCCC
NSEFVKKEQNNHIDLILKSAGELVKFVEYGDIFEKDALEYLKSLKFEVQSTKRDMVFLGC
CHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
THYLHIKDMIESVLEIPVYDNRKIVADELNKSLKNVENSDYCFKRYFYLTQDKNLHFYKN
HHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHH
FCEKYNFYFKGIFN
HHHHCCEEEEEECC
>Mature Secondary Structure
MNNLEDVIVIFDSGIGGLAYFEYISKKLFNRNYIYVADNKNFPYGDKSSNFILNEFLELI
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
SKLREIYNIVSIVVACNTASISVYNKLNFNFPIIYTLPSVSLVKELLCKRVILIATNTTI
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHCEEEEEEECCCC
NSEFVKKEQNNHIDLILKSAGELVKFVEYGDIFEKDALEYLKSLKFEVQSTKRDMVFLGC
CHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
THYLHIKDMIESVLEIPVYDNRKIVADELNKSLKNVENSDYCFKRYFYLTQDKNLHFYKN
HHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHH
FCEKYNFYFKGIFN
HHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]