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The map label for this gene is ldh [H]

Identifier: 203284012

GI number: 203284012

Start: 93872

End: 94834

Strand: Reverse

Name: ldh [H]

Synonym: BDU_90

Alternate gene names: 203284012

Gene position: 94834-93872 (Counterclockwise)

Preceding gene: 203284013

Following gene: 203284008

Centisome position: 10.18

GC content: 31.15

Gene sequence:

>963_bases
ATGGAGATGGTTATGCTTAAACGTAATAAAGTTGTTCTTGTTGGGGCAGGTGGTGTTGGCTCAAGTTTTGCTTATGCTTT
GACAATAGATAACTCACTTGTTCATGAACTTGTAATTATTGATGTAGCTCAAGATAAAGCGAAGGGTGAAGTCATGGATT
TAAACCATGGTCAGATGTTTTTGAAAAAAAATATCAAAATAGAATTTGGTAGTTATGATGATTGTTCTGATGCTGATATT
GTTGTAATTACTGCCGGTCTTAATCAAAAACCAGGTGAGACAAGACTTGATTTGGTTGGCAAGAATACTAAGATTTTTAA
GGAAATTGTAACAAGTATTGTTTCAAGTGGATTTGATGGTATTTTTGTCATTGCAAGTAATCCTGTTGATATTATGACTT
ATGTGACAATGAAGTATTCTAATTTTCCAACCTGTAAGGTTATTGGAACAGGTACAACACTCGATACTTCAAGACTTAGA
TATTTTTTGGCTGAGCGTCTTAATGTTAATACTCAAAATATACATTCATATATTATGGGTGAGCATGGAGATAGTTCTTT
TGCTACTTGGGATGAGACCAAAATAGCTATGAAGTCTTTATCAGAATATATTGCTGATGGGACAATATTAGAATCGGAAC
TTGATGAGATTCATAATAATGTTGTTAATGCTGCTTATGAGGTAATTAAACTTAAAGGTTCAACTTATTATGCTATTGGA
CTTGGAATTAAACGAATTGTTAATGCAATAATTGGTGATCAAAACCTTATTTTACCGATATCTTCATATATTAATGGTCA
GTATGGTGATTCTATTAAAGATATTTATATTGGGGCACCTGCTGTAGTTTGTAAAGATGGTGTAAAAGAAGTCCTTGATT
TTAAAATTAGTGATAGAGAACTTAAAAAATTTCAAATTTCTGCTAATCAATTGAAAAGTTATCTTGATAAAATAGAATTT
TAA

Upstream 100 bases:

>100_bases
ATTAAATACTTAATACAGGGCTTTATAAAAATATTATTGGATTGTTTTAAAGTATCATAAACTGTTATAGAATTACATTA
TACCAATAAAATAATTTTAT

Downstream 100 bases:

>100_bases
TTTTGCCCCAATTGGGGCTTTTTTATTCTTCTAATAGTTTTTCTCCATGTTTTCTGTGCTCAATTGCTTCTAGTAATGAT
TCTCTTGAAATATGAGACTC

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MEMVMLKRNKVVLVGAGGVGSSFAYALTIDNSLVHELVIIDVAQDKAKGEVMDLNHGQMFLKKNIKIEFGSYDDCSDADI
VVITAGLNQKPGETRLDLVGKNTKIFKEIVTSIVSSGFDGIFVIASNPVDIMTYVTMKYSNFPTCKVIGTGTTLDTSRLR
YFLAERLNVNTQNIHSYIMGEHGDSSFATWDETKIAMKSLSEYIADGTILESELDEIHNNVVNAAYEVIKLKGSTYYAIG
LGIKRIVNAIIGDQNLILPISSYINGQYGDSIKDIYIGAPAVVCKDGVKEVLDFKISDRELKKFQISANQLKSYLDKIEF

Sequences:

>Translated_320_residues
MEMVMLKRNKVVLVGAGGVGSSFAYALTIDNSLVHELVIIDVAQDKAKGEVMDLNHGQMFLKKNIKIEFGSYDDCSDADI
VVITAGLNQKPGETRLDLVGKNTKIFKEIVTSIVSSGFDGIFVIASNPVDIMTYVTMKYSNFPTCKVIGTGTTLDTSRLR
YFLAERLNVNTQNIHSYIMGEHGDSSFATWDETKIAMKSLSEYIADGTILESELDEIHNNVVNAAYEVIKLKGSTYYAIG
LGIKRIVNAIIGDQNLILPISSYINGQYGDSIKDIYIGAPAVVCKDGVKEVLDFKISDRELKKFQISANQLKSYLDKIEF
>Mature_320_residues
MEMVMLKRNKVVLVGAGGVGSSFAYALTIDNSLVHELVIIDVAQDKAKGEVMDLNHGQMFLKKNIKIEFGSYDDCSDADI
VVITAGLNQKPGETRLDLVGKNTKIFKEIVTSIVSSGFDGIFVIASNPVDIMTYVTMKYSNFPTCKVIGTGTTLDTSRLR
YFLAERLNVNTQNIHSYIMGEHGDSSFATWDETKIAMKSLSEYIADGTILESELDEIHNNVVNAAYEVIKLKGSTYYAIG
LGIKRIVNAIIGDQNLILPISSYINGQYGDSIKDIYIGAPAVVCKDGVKEVLDFKISDRELKKFQISANQLKSYLDKIEF

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI291575128, Length=312, Percent_Identity=40.3846153846154, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI4557032, Length=312, Percent_Identity=40.3846153846154, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=38.6075949367089, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=38.6075949367089, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI9257228, Length=306, Percent_Identity=38.562091503268, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI4504973, Length=306, Percent_Identity=38.562091503268, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI47059044, Length=308, Percent_Identity=37.012987012987, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI221136809, Length=308, Percent_Identity=37.012987012987, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI15082234, Length=315, Percent_Identity=36.1904761904762, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=41.1764705882353, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI260099725, Length=199, Percent_Identity=42.2110552763819, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI207028494, Length=200, Percent_Identity=38, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI103472011, Length=314, Percent_Identity=26.7515923566879, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI103472015, Length=183, Percent_Identity=32.2404371584699, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI21735621, Length=326, Percent_Identity=25.7668711656442, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789632, Length=325, Percent_Identity=26.7692307692308, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17535107, Length=293, Percent_Identity=38.5665529010239, Blast_Score=214, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17554310, Length=327, Percent_Identity=26.605504587156, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322765, Length=298, Percent_Identity=25.8389261744966, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17136226, Length=315, Percent_Identity=38.4126984126984, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI45550422, Length=310, Percent_Identity=32.9032258064516, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24647881, Length=329, Percent_Identity=27.355623100304, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24663599, Length=318, Percent_Identity=25.4716981132075, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 35315; Mature: 35315

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMVMLKRNKVVLVGAGGVGSSFAYALTIDNSLVHELVIIDVAQDKAKGEVMDLNHGQMF
CCEEEEECCCEEEEECCCCCCCEEEEEEECCHHEEEEEEEEECCCCCCCCEEECCCCEEE
LKKNIKIEFGSYDDCSDADIVVITAGLNQKPGETRLDLVGKNTKIFKEIVTSIVSSGFDG
EEECEEEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCE
IFVIASNPVDIMTYVTMKYSNFPTCKVIGTGTTLDTSRLRYFLAERLNVNTQNIHSYIMG
EEEEECCCCEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHEEC
EHGDSSFATWDETKIAMKSLSEYIADGTILESELDEIHNNVVNAAYEVIKLKGSTYYAIG
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHEEEECCCEEEEEH
LGIKRIVNAIIGDQNLILPISSYINGQYGDSIKDIYIGAPAVVCKDGVKEVLDFKISDRE
HHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCH
LKKFQISANQLKSYLDKIEF
HHHEEECHHHHHHHHHHHCC
>Mature Secondary Structure
MEMVMLKRNKVVLVGAGGVGSSFAYALTIDNSLVHELVIIDVAQDKAKGEVMDLNHGQMF
CCEEEEECCCEEEEECCCCCCCEEEEEEECCHHEEEEEEEEECCCCCCCCEEECCCCEEE
LKKNIKIEFGSYDDCSDADIVVITAGLNQKPGETRLDLVGKNTKIFKEIVTSIVSSGFDG
EEECEEEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCE
IFVIASNPVDIMTYVTMKYSNFPTCKVIGTGTTLDTSRLRYFLAERLNVNTQNIHSYIMG
EEEEECCCCEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHEEC
EHGDSSFATWDETKIAMKSLSEYIADGTILESELDEIHNNVVNAAYEVIKLKGSTYYAIG
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHEEEECCCEEEEEH
LGIKRIVNAIIGDQNLILPISSYINGQYGDSIKDIYIGAPAVVCKDGVKEVLDFKISDRE
HHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCH
LKKFQISANQLKSYLDKIEF
HHHEEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA