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The map label for this gene is lepA [H]

Identifier: 203284013

GI number: 203284013

Start: 95012

End: 96679

Strand: Reverse

Name: lepA [H]

Synonym: BDU_91

Alternate gene names: 203284013

Gene position: 96679-95012 (Counterclockwise)

Preceding gene: 203284014

Following gene: 203284012

Centisome position: 10.38

GC content: 31.53

Gene sequence:

>1668_bases
ATGGATATTGAAAGAGAGAGAGGAATTACCATTAAAAGTCAAGCTGTAACTATTGATTATAAGTGTAGTGATGGCAATAT
TTATGAGCTTAATTTTGTAGATACTCCAGGACATGTTGATTTTTCTTATGAAGTTTCAAGAGCAATTTCATCTTGTGAAG
GGGCTCTTTTACTTGTTGATGCTAGTCAGGGAATAGAAGCTCAAACCGTTTCAAATTTTTATATGGCATTTGAACATAAT
CTTGAAATTATTCCTGTTATTAATAAAATAGATTTGCCAAGTGCAAATATTGATTTTGTGAAAGAGCAAATAGAGAATGA
TTTAGGATTAGATGCTAATGTTGCTGTTCTCATATCTGCTAAGAATGGAATAGGTATTGATGAATTACTTGAGGCTATTT
GTAAGTATGTTCCTTCTCCTAAGGGTAGCAGGGAGAGTCCATTAAAAGCTTTGATTTTTGATTCACATTATGATTCTTAT
CGTGGTGTTGTTGTTCATTTTAGAATTTTTGAGGGACAAATTAGACCCGGTTATAAAATTAAATTTATGCATGCAGATAG
AGAGTATTTTGTGGAAGAGATTGGAATTTTTAAAATTATTCTTGAAAAAAAAGATGTTTTAGAAGCAGGTGATGTTGGAT
ATTTTATTGCAGGAATAAAAAATATATCGGATGTTAAAATTGGAGACACCGTAACTCTTGTTGATAATCCAGCAGTAGCT
CCTCTTGAAGGATTTAAAGAGGTTAAGCCTGTAGTTTTTTCATCTGTTTATCCAGTTGATGCTAATCAATATGATGATCT
ATTGAAAGCAATGGATAGACTTAAGCTAAATGATGCATCTCTTACTTTTGAAAAAGATGCATCAGCTGCTCTTGGCCATG
GATTTAAATGTGGATTTTTGGGACTTTTGCATTTAGAGGTGATTCAGGAAAGAATTGAGCGTGAATTTGATCTTAGTGTA
ATATTAACATCACCATCTGTTCGTTATAAAATTATTCCTAAAAAGGGAAATCCCTATTTTATTGAAAGTCCTGAACAATT
TCCCGGAAATGAAAATATTGAAGTTACACTTGAACCTTATATTAGAGCTAATATTATTGTTCCTGCTGAATTTTTAGGTA
ATATTATGAGCGTTTGTTTACTTAAAAGAGGTGTACAGGAAAATTTAATTTATCTTGATGCGAAGCGTGTTGAAGTTATT
TATAAGATGCCTCTTGCTGAAATATTGTTTGATTTTTATGACAAGATTAAGTCTGTAAGTCGTGGATATGCTTCTTTTGA
TTATACACTATTAGGATATGAGGAAACAGATTTAGTTAAGTTGGATATTTTAGTTAATGGAGATAGAGTTGATGCATTAT
CTCAATTGGTTTTTAGAGATGGTGCACGAGCAAAGGCTTTAAATATTTGTAAAAAATTAAAAGATGAGATTGCAAGACAG
CAATTTAAAATAGCAATTCAAGGAGCCATTGGTTCAAATATTATTGCCCGTGAAACAATTTCACCTGTTAGAAAAGATGT
TACTGCTAAATGTTATGGGGGTGATATTACTCGTAAGAGAAAGCTTTTAGAAAAACAAAAAGAAGGAAAGAAGCGACTTA
AGATGATAGGAAATATTGAAATACCACAAAGTGCATTTCTTGCTGTTCTTAAATCAGATGATAATTAA

Upstream 100 bases:

>100_bases
TGCACATATTGACCATGGTAAATCAACTTTAGCAGATAGGTTTATACAAAAGGCTAAAATAATCTCAGATCGAGAATTTA
AGAGTCAAATTCTTGATAGT

Downstream 100 bases:

>100_bases
TTTATGTTGAGATTAGTATTGAGATAAATTATTTTTTCATTTCAATGTATCAATTTATGCGTTTATTTGCTTTATATATT
AAATACTTAATACAGGGCTT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MDIERERGITIKSQAVTIDYKCSDGNIYELNFVDTPGHVDFSYEVSRAISSCEGALLLVDASQGIEAQTVSNFYMAFEHN
LEIIPVINKIDLPSANIDFVKEQIENDLGLDANVAVLISAKNGIGIDELLEAICKYVPSPKGSRESPLKALIFDSHYDSY
RGVVVHFRIFEGQIRPGYKIKFMHADREYFVEEIGIFKIILEKKDVLEAGDVGYFIAGIKNISDVKIGDTVTLVDNPAVA
PLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDASAALGHGFKCGFLGLLHLEVIQERIEREFDLSV
ILTSPSVRYKIIPKKGNPYFIESPEQFPGNENIEVTLEPYIRANIIVPAEFLGNIMSVCLLKRGVQENLIYLDAKRVEVI
YKMPLAEILFDFYDKIKSVSRGYASFDYTLLGYEETDLVKLDILVNGDRVDALSQLVFRDGARAKALNICKKLKDEIARQ
QFKIAIQGAIGSNIIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRLKMIGNIEIPQSAFLAVLKSDDN

Sequences:

>Translated_555_residues
MDIERERGITIKSQAVTIDYKCSDGNIYELNFVDTPGHVDFSYEVSRAISSCEGALLLVDASQGIEAQTVSNFYMAFEHN
LEIIPVINKIDLPSANIDFVKEQIENDLGLDANVAVLISAKNGIGIDELLEAICKYVPSPKGSRESPLKALIFDSHYDSY
RGVVVHFRIFEGQIRPGYKIKFMHADREYFVEEIGIFKIILEKKDVLEAGDVGYFIAGIKNISDVKIGDTVTLVDNPAVA
PLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDASAALGHGFKCGFLGLLHLEVIQERIEREFDLSV
ILTSPSVRYKIIPKKGNPYFIESPEQFPGNENIEVTLEPYIRANIIVPAEFLGNIMSVCLLKRGVQENLIYLDAKRVEVI
YKMPLAEILFDFYDKIKSVSRGYASFDYTLLGYEETDLVKLDILVNGDRVDALSQLVFRDGARAKALNICKKLKDEIARQ
QFKIAIQGAIGSNIIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRLKMIGNIEIPQSAFLAVLKSDDN
>Mature_555_residues
MDIERERGITIKSQAVTIDYKCSDGNIYELNFVDTPGHVDFSYEVSRAISSCEGALLLVDASQGIEAQTVSNFYMAFEHN
LEIIPVINKIDLPSANIDFVKEQIENDLGLDANVAVLISAKNGIGIDELLEAICKYVPSPKGSRESPLKALIFDSHYDSY
RGVVVHFRIFEGQIRPGYKIKFMHADREYFVEEIGIFKIILEKKDVLEAGDVGYFIAGIKNISDVKIGDTVTLVDNPAVA
PLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDASAALGHGFKCGFLGLLHLEVIQERIEREFDLSV
ILTSPSVRYKIIPKKGNPYFIESPEQFPGNENIEVTLEPYIRANIIVPAEFLGNIMSVCLLKRGVQENLIYLDAKRVEVI
YKMPLAEILFDFYDKIKSVSRGYASFDYTLLGYEETDLVKLDILVNGDRVDALSQLVFRDGARAKALNICKKLKDEIARQ
QFKIAIQGAIGSNIIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRLKMIGNIEIPQSAFLAVLKSDDN

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=563, Percent_Identity=48.8454706927176, Blast_Score=568, Evalue=1e-162,
Organism=Homo sapiens, GI25306287, Length=108, Percent_Identity=45.3703703703704, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI25306283, Length=108, Percent_Identity=45.3703703703704, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI19923640, Length=108, Percent_Identity=45.3703703703704, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=120, Percent_Identity=37.5, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI18390331, Length=112, Percent_Identity=38.3928571428571, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI4503483, Length=99, Percent_Identity=37.3737373737374, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI217272894, Length=88, Percent_Identity=39.7727272727273, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI217272892, Length=88, Percent_Identity=39.7727272727273, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI94966752, Length=96, Percent_Identity=37.5, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788922, Length=550, Percent_Identity=51.2727272727273, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI48994988, Length=461, Percent_Identity=28.4164859002169, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789738, Length=113, Percent_Identity=35.3982300884956, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789559, Length=216, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=570, Percent_Identity=40, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17556745, Length=416, Percent_Identity=24.5192307692308, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=138, Percent_Identity=34.7826086956522, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=35.3383458646617, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=561, Percent_Identity=42.7807486631016, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6323098, Length=138, Percent_Identity=34.7826086956522, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=112, Percent_Identity=37.5, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=112, Percent_Identity=37.5, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322359, Length=69, Percent_Identity=42.0289855072464, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=236, Percent_Identity=25.8474576271186, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI78706572, Length=561, Percent_Identity=40.6417112299465, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24582462, Length=112, Percent_Identity=38.3928571428571, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI221458488, Length=98, Percent_Identity=42.8571428571429, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574573, Length=92, Percent_Identity=42.3913043478261, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 62180; Mature: 62180

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIERERGITIKSQAVTIDYKCSDGNIYELNFVDTPGHVDFSYEVSRAISSCEGALLLVD
CCCCCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEE
ASQGIEAQTVSNFYMAFEHNLEIIPVINKIDLPSANIDFVKEQIENDLGLDANVAVLISA
CCCCCCHHHHHHEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEC
KNGIGIDELLEAICKYVPSPKGSRESPLKALIFDSHYDSYRGVVVHFRIFEGQIRPGYKI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCEEE
KFMHADREYFVEEIGIFKIILEKKDVLEAGDVGYFIAGIKNISDVKIGDTVTLVDNPAVA
EEEECCHHHHHHHHCEEEEEECHHHHHCCCCCCEEEECCCCCCCEEECCEEEEECCCCCC
PLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDASAALGHGFKCGFL
CHHHHHHHCHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCCCCHHHH
GLLHLEVIQERIEREFDLSVILTSPSVRYKIIPKKGNPYFIESPEQFPGNENIEVTLEPY
HHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCCCEEEEECCCCCCCCCCEEEEEECC
IRANIIVPAEFLGNIMSVCLLKRGVQENLIYLDAKRVEVIYKMPLAEILFDFYDKIKSVS
EEEEEEECHHHHHHHHHHHHHHCCCHHCEEEEEHHHEEEEEECCHHHHHHHHHHHHHHHH
RGYASFDYTLLGYEETDLVKLDILVNGDRVDALSQLVFRDGARAKALNICKKLKDEIARQ
CCCCCCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QFKIAIQGAIGSNIIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRLKMIGNIE
HEEEEEEECCCCCEEHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
IPQSAFLAVLKSDDN
CCHHHEEEEEECCCC
>Mature Secondary Structure
MDIERERGITIKSQAVTIDYKCSDGNIYELNFVDTPGHVDFSYEVSRAISSCEGALLLVD
CCCCCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEE
ASQGIEAQTVSNFYMAFEHNLEIIPVINKIDLPSANIDFVKEQIENDLGLDANVAVLISA
CCCCCCHHHHHHEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEC
KNGIGIDELLEAICKYVPSPKGSRESPLKALIFDSHYDSYRGVVVHFRIFEGQIRPGYKI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCEEE
KFMHADREYFVEEIGIFKIILEKKDVLEAGDVGYFIAGIKNISDVKIGDTVTLVDNPAVA
EEEECCHHHHHHHHCEEEEEECHHHHHCCCCCCEEEECCCCCCCEEECCEEEEECCCCCC
PLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDASAALGHGFKCGFL
CHHHHHHHCHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCCCCHHHH
GLLHLEVIQERIEREFDLSVILTSPSVRYKIIPKKGNPYFIESPEQFPGNENIEVTLEPY
HHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCCCEEEEECCCCCCCCCCEEEEEECC
IRANIIVPAEFLGNIMSVCLLKRGVQENLIYLDAKRVEVIYKMPLAEILFDFYDKIKSVS
EEEEEEECHHHHHHHHHHHHHHCCCHHCEEEEEHHHEEEEEECCHHHHHHHHHHHHHHHH
RGYASFDYTLLGYEETDLVKLDILVNGDRVDALSQLVFRDGARAKALNICKKLKDEIARQ
CCCCCCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QFKIAIQGAIGSNIIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRLKMIGNIE
HEEEEEEECCCCCEEHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
IPQSAFLAVLKSDDN
CCHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]