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The map label for this gene is gap [H]

Identifier: 203283983

GI number: 203283983

Start: 63361

End: 64368

Strand: Reverse

Name: gap [H]

Synonym: BDU_61

Alternate gene names: 203283983

Gene position: 64368-63361 (Counterclockwise)

Preceding gene: 203283984

Following gene: 203283982

Centisome position: 6.91

GC content: 35.12

Gene sequence:

>1008_bases
ATGAAGTTAGCTATTAATGGCTTTGGACGTATAGGCAGAAATGTTTTTAAAATTGCTTTTGAGAGAGGGATAGAAGTTGT
TGCTGTAAATGATTTGACAGATCCTAAAACACTTGCACACCTTTTGAAATATGATTCTACTTTTGGAGTATATAATAAAA
AGGTTGAAGCAAGAGATGGTGCTATTGTAGTAGATGGAAAAGAAATAAAGATTATTGCTGAGCGTGATCCCAAAAAGCTT
CCTTGGGGTAAATTGGGAATTGATGTTGTAATTGAGTCAACAGGTGTTTTTACTTCAGCAACAAGTGATAGAGGTGGGTA
TCTTGATCATGTTGAATATGCTGGTGCTAAGAAGGTAATTTTGACAGTGCCTGCTAAAGATGATATTAAAACAATTGTAC
TTGGTGTTAATGAGCATGAAATTACTTCTGATTTAAAAGCTGTTTCTAATGCTTCATGCACAACAAACTGTTTGGCACCT
CTTGCAAAGGTTTTACATGAGGCTTTTGGTATTGAGAAAGGTCTTATGACTACTGTGCATGCTTATACTAATGATCAAAA
GATTTTGGATTTGCCACATGCTGATTTAAGACGAGCACGAGCTGGGGCTCTTTCAATTATTCCTACTTCAACAGGTGCGG
CTAAGGCTGTTGGGCTTGTTTTGCCTGAACTTAAAGGTAAGCTTAATGGTACTTCTATGAGAGTTCCTGTACCAACAGGT
TCTATTGTTGATCTTACTGTTCAACTTAAGAAAAAAGATGTTACAAAAGAAGAGATCAATTCTGTACTTAAGAAAGCATC
AGAGTCTAGAGAACTTAGTGGTATTTTAGGATATACAGAAGATCCGATAGTGTCTTCAGATATTAAAGGAAATTCTCATT
CTTCAATAGTTGATGGTCTTGAGACTATGGTATTGTTAGATGGTTTTGTGAAAGTACTTTCTTGGTATGACAATGAATTT
GGATATTCTACAAGAGTAGTTGATCTTGCACAAAAATTAGTTAAATAA

Upstream 100 bases:

>100_bases
AGGATATACGGTTTTTATAAAAAAATAGTTTGACACTTGGTATTAATTAATTTATTATTTAATAATTCTAGTAGACAATA
AAAAATGATGGAGGAGTTGT

Downstream 100 bases:

>100_bases
TTTATAAGATAATCTTGAGGTATTTGTAAATGTCAATAAGAACTATAAAAGATTGTGATTTTTCAGGCAAACGTGCTTTG
GTTAGATGTGATTTTAATGT

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKLAINGFGRIGRNVFKIAFERGIEVVAVNDLTDPKTLAHLLKYDSTFGVYNKKVEARDGAIVVDGKEIKIIAERDPKKL
PWGKLGIDVVIESTGVFTSATSDRGGYLDHVEYAGAKKVILTVPAKDDIKTIVLGVNEHEITSDLKAVSNASCTTNCLAP
LAKVLHEAFGIEKGLMTTVHAYTNDQKILDLPHADLRRARAGALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
SIVDLTVQLKKKDVTKEEINSVLKKASESRELSGILGYTEDPIVSSDIKGNSHSSIVDGLETMVLLDGFVKVLSWYDNEF
GYSTRVVDLAQKLVK

Sequences:

>Translated_335_residues
MKLAINGFGRIGRNVFKIAFERGIEVVAVNDLTDPKTLAHLLKYDSTFGVYNKKVEARDGAIVVDGKEIKIIAERDPKKL
PWGKLGIDVVIESTGVFTSATSDRGGYLDHVEYAGAKKVILTVPAKDDIKTIVLGVNEHEITSDLKAVSNASCTTNCLAP
LAKVLHEAFGIEKGLMTTVHAYTNDQKILDLPHADLRRARAGALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
SIVDLTVQLKKKDVTKEEINSVLKKASESRELSGILGYTEDPIVSSDIKGNSHSSIVDGLETMVLLDGFVKVLSWYDNEF
GYSTRVVDLAQKLVK
>Mature_335_residues
MKLAINGFGRIGRNVFKIAFERGIEVVAVNDLTDPKTLAHLLKYDSTFGVYNKKVEARDGAIVVDGKEIKIIAERDPKKL
PWGKLGIDVVIESTGVFTSATSDRGGYLDHVEYAGAKKVILTVPAKDDIKTIVLGVNEHEITSDLKAVSNASCTTNCLAP
LAKVLHEAFGIEKGLMTTVHAYTNDQKILDLPHADLRRARAGALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
SIVDLTVQLKKKDVTKEEINSVLKKASESRELSGILGYTEDPIVSSDIKGNSHSSIVDGLETMVLLDGFVKVLSWYDNEF
GYSTRVVDLAQKLVK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=336, Percent_Identity=51.1904761904762, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI7657116, Length=339, Percent_Identity=49.2625368731563, Blast_Score=314, Evalue=8e-86,
Organism=Escherichia coli, GI1788079, Length=337, Percent_Identity=52.2255192878338, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1789295, Length=333, Percent_Identity=41.4414414414414, Blast_Score=268, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17534677, Length=337, Percent_Identity=48.9614243323442, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17534679, Length=337, Percent_Identity=48.6646884272997, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32566163, Length=337, Percent_Identity=48.0712166172107, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=48.0712166172107, Blast_Score=271, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6321631, Length=338, Percent_Identity=55.0295857988166, Blast_Score=339, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6322468, Length=338, Percent_Identity=54.7337278106509, Blast_Score=338, Evalue=8e-94,
Organism=Saccharomyces cerevisiae, GI6322409, Length=338, Percent_Identity=52.6627218934911, Blast_Score=324, Evalue=1e-89,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=50.2994011976048, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=50.2994011976048, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=49.7005988023952, Blast_Score=301, Evalue=3e-82,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=49.7005988023952, Blast_Score=301, Evalue=3e-82,
Organism=Drosophila melanogaster, GI19922412, Length=330, Percent_Identity=48.7878787878788, Blast_Score=298, Evalue=3e-81,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36182; Mature: 36182

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAINGFGRIGRNVFKIAFERGIEVVAVNDLTDPKTLAHLLKYDSTFGVYNKKVEARDG
CEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEECCCC
AIVVDGKEIKIIAERDPKKLPWGKLGIDVVIESTGVFTSATSDRGGYLDHVEYAGAKKVI
EEEECCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCHHHHCCCCEEE
LTVPAKDDIKTIVLGVNEHEITSDLKAVSNASCTTNCLAPLAKVLHEAFGIEKGLMTTVH
EECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEE
AYTNDQKILDLPHADLRRARAGALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
EECCCCEEEECCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCC
SIVDLTVQLKKKDVTKEEINSVLKKASESRELSGILGYTEDPIVSSDIKGNSHSSIVDGL
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
ETMVLLDGFVKVLSWYDNEFGYSTRVVDLAQKLVK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKLAINGFGRIGRNVFKIAFERGIEVVAVNDLTDPKTLAHLLKYDSTFGVYNKKVEARDG
CEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEECCCC
AIVVDGKEIKIIAERDPKKLPWGKLGIDVVIESTGVFTSATSDRGGYLDHVEYAGAKKVI
EEEECCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCHHHHCCCCEEE
LTVPAKDDIKTIVLGVNEHEITSDLKAVSNASCTTNCLAPLAKVLHEAFGIEKGLMTTVH
EECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEE
AYTNDQKILDLPHADLRRARAGALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
EECCCCEEEECCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCC
SIVDLTVQLKKKDVTKEEINSVLKKASESRELSGILGYTEDPIVSSDIKGNSHSSIVDGL
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
ETMVLLDGFVKVLSWYDNEFGYSTRVVDLAQKLVK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8557349; 9403685 [H]