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The map label for this gene is pgk

Identifier: 203283982

GI number: 203283982

Start: 62153

End: 63331

Strand: Reverse

Name: pgk

Synonym: BDU_60

Alternate gene names: 203283982

Gene position: 63331-62153 (Counterclockwise)

Preceding gene: 203283983

Following gene: 203283981

Centisome position: 6.8

GC content: 35.03

Gene sequence:

>1179_bases
ATGTCAATAAGAACTATAAAAGATTGTGATTTTTCAGGCAAACGTGCTTTGGTTAGATGTGATTTTAATGTTCCCTTACG
AGAAGGGAACATTACTGATGATACTAGAATTAAGGCGGCTTTACCCACAATAGAATATCTTAAATCTCAAGGAGCCAGAG
TTGTTTTGATGAGTCATTTGGGTAGACCAAAGGGAGAGAAAAATCTTAGGTATTCTCTTATGCCTATTGCTAGGAGATTA
TCAGAACTGTTGGGGCAAAATGTTAAGATGTTGTCTGATTGTATAGGTGATGAGGTAGTTACAGCTGTTTCTTGTATGCA
AAATGGAGATGTTGTTTTACTTGAAAATGTGAGGTTTTATAAGGAAGAAGAAGAAAATAGTGATGCTTTTGCAATGCAGT
TATCAAAAAGTGGTGATATTTTTGTAAATGATGCTTTTGGAACTGCTCATAGAGCTCATGCTTCTACATCAGGTGTTGCA
TCTTATTTGCCAGCCGTTGGTGGATTTTTAATGGAAAGAGAAGATGAATTTTTGGGTAAAATTTTAAAAAATCCTGAGAG
TCCATTTGTTTCAATAATTGGTGGTTCAAAAGTTTCTTCAAAAATTGCAGTTCTTGAATCCCTTTTACCAAAATCAAATG
TGATGGTAATTGGTGGTGGAATGGCATATACCTTTTTAAAAGTAGAGGGATATTCTATTGGTAAATCTCTTTTAGAAAAT
GAATATATTGATGTAGCCTCATCTTTTTTGAAGAAGGCAAAGGAATTAAGTGTAGAAGTTATTTTGCCTATTGATCATGT
TGTTGCAAGTGAATTTCAGGAATATTCTATGCCTGAATATGTTGACTCTGTTAATATTCCTGATAGTAAGATTGGGATGG
ATATTGGAGAGAAGACTTTGAAAAAAATTGAGGGAGTTCTTAGTAGTGCAAAAACTGTCATTTGGAATGGTCCTCTTGGA
GTATTTGAGTTTGATTCTTTTGCCAAAGGTACAGCAAAGGTTGCAGAGTATGTGGCTAATTGTCCTGGAATTACGGTTGT
TGGAGGTGGAGATTCAGTTGCTGCTGTAAATAAATTTAATTTGTCTGGAAAGATAACTCATGTTTCAACAGGGGGTGGTG
CTTCTCTTGAATATCTTGAAGGAAAAGTTTTGCCAGGTATAAAGGTGTTGGAGGTTTAA

Upstream 100 bases:

>100_bases
TTTCTTGGTATGACAATGAATTTGGATATTCTACAAGAGTAGTTGATCTTGCACAAAAATTAGTTAAATAATTTATAAGA
TAATCTTGAGGTATTTGTAA

Downstream 100 bases:

>100_bases
GATGAGAAAGGTTTTCTTAGCAGGAAATTGGAAAATGCATTATACAAGTGTAGAGGCCGCAGATGTTGCTAAGCAGATTG
TAGATGGTGTATACAATATT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSIRTIKDCDFSGKRALVRCDFNVPLREGNITDDTRIKAALPTIEYLKSQGARVVLMSHLGRPKGEKNLRYSLMPIARRL
SELLGQNVKMLSDCIGDEVVTAVSCMQNGDVVLLENVRFYKEEEENSDAFAMQLSKSGDIFVNDAFGTAHRAHASTSGVA
SYLPAVGGFLMEREDEFLGKILKNPESPFVSIIGGSKVSSKIAVLESLLPKSNVMVIGGGMAYTFLKVEGYSIGKSLLEN
EYIDVASSFLKKAKELSVEVILPIDHVVASEFQEYSMPEYVDSVNIPDSKIGMDIGEKTLKKIEGVLSSAKTVIWNGPLG
VFEFDSFAKGTAKVAEYVANCPGITVVGGGDSVAAVNKFNLSGKITHVSTGGGASLEYLEGKVLPGIKVLEV

Sequences:

>Translated_392_residues
MSIRTIKDCDFSGKRALVRCDFNVPLREGNITDDTRIKAALPTIEYLKSQGARVVLMSHLGRPKGEKNLRYSLMPIARRL
SELLGQNVKMLSDCIGDEVVTAVSCMQNGDVVLLENVRFYKEEEENSDAFAMQLSKSGDIFVNDAFGTAHRAHASTSGVA
SYLPAVGGFLMEREDEFLGKILKNPESPFVSIIGGSKVSSKIAVLESLLPKSNVMVIGGGMAYTFLKVEGYSIGKSLLEN
EYIDVASSFLKKAKELSVEVILPIDHVVASEFQEYSMPEYVDSVNIPDSKIGMDIGEKTLKKIEGVLSSAKTVIWNGPLG
VFEFDSFAKGTAKVAEYVANCPGITVVGGGDSVAAVNKFNLSGKITHVSTGGGASLEYLEGKVLPGIKVLEV
>Mature_391_residues
SIRTIKDCDFSGKRALVRCDFNVPLREGNITDDTRIKAALPTIEYLKSQGARVVLMSHLGRPKGEKNLRYSLMPIARRLS
ELLGQNVKMLSDCIGDEVVTAVSCMQNGDVVLLENVRFYKEEEENSDAFAMQLSKSGDIFVNDAFGTAHRAHASTSGVAS
YLPAVGGFLMEREDEFLGKILKNPESPFVSIIGGSKVSSKIAVLESLLPKSNVMVIGGGMAYTFLKVEGYSIGKSLLENE
YIDVASSFLKKAKELSVEVILPIDHVVASEFQEYSMPEYVDSVNIPDSKIGMDIGEKTLKKIEGVLSSAKTVIWNGPLGV
FEFDSFAKGTAKVAEYVANCPGITVVGGGDSVAAVNKFNLSGKITHVSTGGGASLEYLEGKVLPGIKVLEV

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=409, Percent_Identity=45.2322738386308, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=44.7432762836186, Blast_Score=338, Evalue=7e-93,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=43.5443037974684, Blast_Score=324, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=47.1744471744472, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319857, Length=407, Percent_Identity=44.963144963145, Blast_Score=338, Evalue=6e-94,
Organism=Drosophila melanogaster, GI17136394, Length=407, Percent_Identity=45.4545454545455, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24581203, Length=408, Percent_Identity=43.8725490196078, Blast_Score=340, Evalue=9e-94,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_BORDL (B5RKV5)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221722.1
- EnsemblBacteria:   EBBORT00000001657
- GeneID:   6918144
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_60
- GeneTree:   EBGT00050000007101
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42296; Mature: 42165

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 292-292 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRTIKDCDFSGKRALVRCDFNVPLREGNITDDTRIKAALPTIEYLKSQGARVVLMSHL
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCEEEEEHHC
GRPKGEKNLRYSLMPIARRLSELLGQNVKMLSDCIGDEVVTAVSCMQNGDVVLLENVRFY
CCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEH
KEEEENSDAFAMQLSKSGDIFVNDAFGTAHRAHASTSGVASYLPAVGGFLMEREDEFLGK
HCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILKNPESPFVSIIGGSKVSSKIAVLESLLPKSNVMVIGGGMAYTFLKVEGYSIGKSLLEN
HHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEEEECCHHHHHHHHH
EYIDVASSFLKKAKELSVEVILPIDHVVASEFQEYSMPEYVDSVNIPDSKIGMDIGEKTL
HHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHCCCHHHHHCCCCCCHHHCCCHHHHHH
KKIEGVLSSAKTVIWNGPLGVFEFDSFAKGTAKVAEYVANCPGITVVGGGDSVAAVNKFN
HHHHHHHHCCCEEEECCCCCCEECCHHCCHHHHHHHHHHCCCCEEEECCCCCEEEEEEEC
LSGKITHVSTGGGASLEYLEGKVLPGIKVLEV
CCEEEEEEECCCCCEEHHHCCCCCCCEEEEEC
>Mature Secondary Structure 
SIRTIKDCDFSGKRALVRCDFNVPLREGNITDDTRIKAALPTIEYLKSQGARVVLMSHL
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCEEEEEHHC
GRPKGEKNLRYSLMPIARRLSELLGQNVKMLSDCIGDEVVTAVSCMQNGDVVLLENVRFY
CCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEH
KEEEENSDAFAMQLSKSGDIFVNDAFGTAHRAHASTSGVASYLPAVGGFLMEREDEFLGK
HCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILKNPESPFVSIIGGSKVSSKIAVLESLLPKSNVMVIGGGMAYTFLKVEGYSIGKSLLEN
HHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEEEECCHHHHHHHHH
EYIDVASSFLKKAKELSVEVILPIDHVVASEFQEYSMPEYVDSVNIPDSKIGMDIGEKTL
HHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHCCCHHHHHCCCCCCHHHCCCHHHHHH
KKIEGVLSSAKTVIWNGPLGVFEFDSFAKGTAKVAEYVANCPGITVVGGGDSVAAVNKFN
HHHHHHHHCCCEEEECCCCCCEECCHHCCHHHHHHHHHHCCCCEEEECCCCCEEEEEEEC
LSGKITHVSTGGGASLEYLEGKVLPGIKVLEV
CCEEEEEEECCCCCEEHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA