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The map label for this gene is tpiA

Identifier: 203283981

GI number: 203283981

Start: 61387

End: 62151

Strand: Reverse

Name: tpiA

Synonym: BDU_59

Alternate gene names: 203283981

Gene position: 62151-61387 (Counterclockwise)

Preceding gene: 203283982

Following gene: 203283980

Centisome position: 6.67

GC content: 33.2

Gene sequence:

>765_bases
ATGAGAAAGGTTTTCTTAGCAGGAAATTGGAAAATGCATTATACAAGTGTAGAGGCCGCAGATGTTGCTAAGCAGATTGT
AGATGGTGTATACAATATTAATAATAATGTGGTTGTTATGGTAACACCTACATTTACGTCCCTTTGTAAAGTTTGTAGAG
TAACTAAAGGAACCAATGTTCTTCTTGGTGCTCAAAATATGTCTTATGAGAATAGCGGAGCTAGAACAAGTGAAATTGCG
CCTTCTATGCTTTTGGAATTTGGGGTTGATTACGTAATACTTGGACATTCTGAATGTAGAACATATCTTGGCGAAAATGA
TGAAATTATAAATAAGAAAGTTCTTACAGGTCTTAAACATCCATTTAAATATTTAATTCTCTGTGTTGGAGAAACTCTTG
AAGAGAGAGAAAAAGGTAAAACTTTAGATGTAGTTTTGAATCAGGTTAGAAATGGATTAGCATCTGTATATGAATCTGAT
CTTCAGAGAATAATTTTAGCTTATGAACCTGTATGGGCAATTGGGACAGGAAAGACAGCAACAAAAGAAGAAGCACAAGA
AGTTCATAAGGCAATTAGACTTGAAATTCAATCGTTATATTCAAAGTCCGCAGCAGATAATATTATTATTCAATATGGTG
GTTCTGTTAATGTTGACAATGTGGAAGGTTTAATGGGTGAAAACGATATTGATGGAGCATTAATTGGTGGTGCATCTTTA
AAAGCAGATTCGTTTTTAAAGATAATTAATAAAATATCGAAGTAG

Upstream 100 bases:

>100_bases
TTGTCTGGAAAGATAACTCATGTTTCAACAGGGGGTGGTGCTTCTCTTGAATATCTTGAAGGAAAAGTTTTGCCAGGTAT
AAAGGTGTTGGAGGTTTAAG

Downstream 100 bases:

>100_bases
AGAGGTTGATTTTGGAGTTAGTTAGGTTTTTGGTATTTATCTTTTTTGTTATTACTTCATTTATGATTATTTTGTTAGTA
TTGTTTCAAGATGAGCAAGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRKVFLAGNWKMHYTSVEAADVAKQIVDGVYNINNNVVVMVTPTFTSLCKVCRVTKGTNVLLGAQNMSYENSGARTSEIA
PSMLLEFGVDYVILGHSECRTYLGENDEIINKKVLTGLKHPFKYLILCVGETLEEREKGKTLDVVLNQVRNGLASVYESD
LQRIILAYEPVWAIGTGKTATKEEAQEVHKAIRLEIQSLYSKSAADNIIIQYGGSVNVDNVEGLMGENDIDGALIGGASL
KADSFLKIINKISK

Sequences:

>Translated_254_residues
MRKVFLAGNWKMHYTSVEAADVAKQIVDGVYNINNNVVVMVTPTFTSLCKVCRVTKGTNVLLGAQNMSYENSGARTSEIA
PSMLLEFGVDYVILGHSECRTYLGENDEIINKKVLTGLKHPFKYLILCVGETLEEREKGKTLDVVLNQVRNGLASVYESD
LQRIILAYEPVWAIGTGKTATKEEAQEVHKAIRLEIQSLYSKSAADNIIIQYGGSVNVDNVEGLMGENDIDGALIGGASL
KADSFLKIINKISK
>Mature_254_residues
MRKVFLAGNWKMHYTSVEAADVAKQIVDGVYNINNNVVVMVTPTFTSLCKVCRVTKGTNVLLGAQNMSYENSGARTSEIA
PSMLLEFGVDYVILGHSECRTYLGENDEIINKKVLTGLKHPFKYLILCVGETLEEREKGKTLDVVLNQVRNGLASVYESD
LQRIILAYEPVWAIGTGKTATKEEAQEVHKAIRLEIQSLYSKSAADNIIIQYGGSVNVDNVEGLMGENDIDGALIGGASL
KADSFLKIINKISK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=39.7590361445783, Blast_Score=164, Evalue=5e-41,
Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=39.7590361445783, Blast_Score=164, Evalue=5e-41,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=41.6, Blast_Score=185, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17536593, Length=260, Percent_Identity=44.2307692307692, Blast_Score=183, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=40.5622489959839, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28572008, Length=254, Percent_Identity=42.1259842519685, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28572006, Length=254, Percent_Identity=42.1259842519685, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28572004, Length=254, Percent_Identity=42.1259842519685, Blast_Score=178, Evalue=3e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_BORDL (B5RKV4)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221721.1
- EnsemblBacteria:   EBBORT00000000979
- GeneID:   6918143
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_59
- GeneTree:   EBGT00050000007284
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27879; Mature: 27879

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 169-169 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVFLAGNWKMHYTSVEAADVAKQIVDGVYNINNNVVVMVTPTFTSLCKVCRVTKGTNV
CCEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEE
LLGAQNMSYENSGARTSEIAPSMLLEFGVDYVILGHSECRTYLGENDEIINKKVLTGLKH
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHH
PFKYLILCVGETLEEREKGKTLDVVLNQVRNGLASVYESDLQRIILAYEPVWAIGTGKTA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
TKEEAQEVHKAIRLEIQSLYSKSAADNIIIQYGGSVNVDNVEGLMGENDIDGALIGGASL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCCCCEEECCCCC
KADSFLKIINKISK
CHHHHHHHHHHHCC
>Mature Secondary Structure
MRKVFLAGNWKMHYTSVEAADVAKQIVDGVYNINNNVVVMVTPTFTSLCKVCRVTKGTNV
CCEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEE
LLGAQNMSYENSGARTSEIAPSMLLEFGVDYVILGHSECRTYLGENDEIINKKVLTGLKH
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHH
PFKYLILCVGETLEEREKGKTLDVVLNQVRNGLASVYESDLQRIILAYEPVWAIGTGKTA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
TKEEAQEVHKAIRLEIQSLYSKSAADNIIIQYGGSVNVDNVEGLMGENDIDGALIGGASL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCCCCEEECCCCC
KADSFLKIINKISK
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA