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The map label for this gene is pfpB [H]

Identifier: 203283950

GI number: 203283950

Start: 30307

End: 31971

Strand: Reverse

Name: pfpB [H]

Synonym: BDU_26

Alternate gene names: 203283950

Gene position: 31971-30307 (Counterclockwise)

Preceding gene: 203283951

Following gene: 203283949

Centisome position: 3.43

GC content: 30.33

Gene sequence:

>1665_bases
ATGATTTCAATTTTGCAAAAGGAAAGATATAAGTATGTTCCAAGGTTGCCAAAAATTTTAACAAATGATTTTCAAAACAT
TAGTGTAGTTTTTGGTGACAAGACAGATGCTCTTGGAAATAAAGATGAGTTAAGAGAAGTTTTTGAAAATACTTATGGAC
TTCCGGTTGTTAATTTTATTCAAGGTTCTTCGAATGTAGATTTTACAAAAATTTTAAATGTAGGAATAATTCTTTCAGGT
GGGCCTGCTCCTGGAGGACATAATGTTGTTGCTGGTATTTTTGATGCAATAAAAAAATCTAATTCAAATTCAAAAATTTT
TGGTTTTAAAGGTGGTCCTGCAGGTTTGTTAGATGATAAAAAAATTGAAATTACACAAGATCTAATAGATGCTTATAAGA
ACACAGGTGGTTTTGATATTGTATCTTCTGGTCGCACTAAAATAGAAACCTATGTTCAATATGAGCAAGTTTTGTCAGTA
GTTCTTAAAAATAATCTTAATGCTCTTATTATTATTGGTGGCGATGATTCAAATACTAATGCCGCTTTATTAGCAGAGTT
TTTTAAGAAAAAACATCATGATATTCAAGTTATTGGTGTCCCTAAAACAATTGATGCTGATTTGAGGAATGAGCATATTC
AAATTTCATTTGGATTTGATTCTGCTACTAAAACTTATTCTGAAATGGTAGGTAATTTATGTCGTGATGCTATGTCTGCT
GGAAAATATTGGCATTTTATAAAGATAATGGGAAGAAGTGCGTCTCATGTTGCTCTTGAGTGTGCTTTAAAGACGCATCC
TAATATTTGTATTATATCTGAAGAAGTTTTGGCAAAAAATAAAACTTTATCAGAAATTGTTGAAGATATAGCGTCTGTTG
TTATAAAGCGTTCATTGAAAGGGTATAATTTTGGTGTTATTATAATTCCTGAAGGAGTTATTGAATTTATTCCTGAAGTT
AAATCTTTGATGATTGAATTGTGTAGTATCTTTGATAGTAATGAAGAGGAATTTAAGGGATTAGATGTTGAGGATATAAG
AAAAGTTTTTATCTCTAAGCTTAGTGAGTATATGAAAAAAGTTTATGTGTCTTTGCCATTATTTATTCAAATTGAACTTG
TAAATTCTGTTTTAGAGAGAGATCCTCATGGTAATTTTCATGTGTCTCGAGTGCCTACTGAGAAGTTATTTATGGAAATG
GTTAGTGTTAGATTAGAAGAGTTAAGAAATCTTGGTGAATATAGTGGTAAATTTTCTCCAATTGATCATTTTTTTGGTTA
TGAGGGTAGAAGTGTTACTCCTTCAAATTTTGATAGTGATTATTGTTATAGTTTGGGTTATAATGCTGCATTGCTTGTTT
TAAATGGTTTTACAGGCTATATGTCTACTATTAAAAATTTAAATAAAAATTCTACTGAATGGATTGCAGGTGGGGTGCCT
ATAACAATGATGATGAATATGGAGGAGAGATATGGTGTTTTAAAACCTGTTATTAAAAAAGCTCTTGTTGATTTAAATGG
AGCACCTTTTAATGAATTTGTGAAGCATCGTGAACAATGGGCAATCAATAATTTATATGTTTTTCCAGGGCCTATTCAAT
ATTTTGGTGCATCTGAACTTGTTGATGAGATAACTTTAACATTGAAGTTAGAATTGGAAACATGA

Upstream 100 bases:

>100_bases
TGATGCTACTTTATTTTTAAGTCATATATAGATATTAATGATTAATTATTTTAGTATTTACTTAATAATAATTTAGATAA
ATCTTGAAAGGAGAAGATAT

Downstream 100 bases:

>100_bases
ATTATTAATGATGATTATATTTTTGCTTTTTATTTGCAATATTTTTATCCTTTATTCAAATTCTTTAGGTTATACTAAAT
TAGATTTTGAATATTTAAAT

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MISILQKERYKYVPRLPKILTNDFQNISVVFGDKTDALGNKDELREVFENTYGLPVVNFIQGSSNVDFTKILNVGIILSG
GPAPGGHNVVAGIFDAIKKSNSNSKIFGFKGGPAGLLDDKKIEITQDLIDAYKNTGGFDIVSSGRTKIETYVQYEQVLSV
VLKNNLNALIIIGGDDSNTNAALLAEFFKKKHHDIQVIGVPKTIDADLRNEHIQISFGFDSATKTYSEMVGNLCRDAMSA
GKYWHFIKIMGRSASHVALECALKTHPNICIISEEVLAKNKTLSEIVEDIASVVIKRSLKGYNFGVIIIPEGVIEFIPEV
KSLMIELCSIFDSNEEEFKGLDVEDIRKVFISKLSEYMKKVYVSLPLFIQIELVNSVLERDPHGNFHVSRVPTEKLFMEM
VSVRLEELRNLGEYSGKFSPIDHFFGYEGRSVTPSNFDSDYCYSLGYNAALLVLNGFTGYMSTIKNLNKNSTEWIAGGVP
ITMMMNMEERYGVLKPVIKKALVDLNGAPFNEFVKHREQWAINNLYVFPGPIQYFGASELVDEITLTLKLELET

Sequences:

>Translated_554_residues
MISILQKERYKYVPRLPKILTNDFQNISVVFGDKTDALGNKDELREVFENTYGLPVVNFIQGSSNVDFTKILNVGIILSG
GPAPGGHNVVAGIFDAIKKSNSNSKIFGFKGGPAGLLDDKKIEITQDLIDAYKNTGGFDIVSSGRTKIETYVQYEQVLSV
VLKNNLNALIIIGGDDSNTNAALLAEFFKKKHHDIQVIGVPKTIDADLRNEHIQISFGFDSATKTYSEMVGNLCRDAMSA
GKYWHFIKIMGRSASHVALECALKTHPNICIISEEVLAKNKTLSEIVEDIASVVIKRSLKGYNFGVIIIPEGVIEFIPEV
KSLMIELCSIFDSNEEEFKGLDVEDIRKVFISKLSEYMKKVYVSLPLFIQIELVNSVLERDPHGNFHVSRVPTEKLFMEM
VSVRLEELRNLGEYSGKFSPIDHFFGYEGRSVTPSNFDSDYCYSLGYNAALLVLNGFTGYMSTIKNLNKNSTEWIAGGVP
ITMMMNMEERYGVLKPVIKKALVDLNGAPFNEFVKHREQWAINNLYVFPGPIQYFGASELVDEITLTLKLELET
>Mature_554_residues
MISILQKERYKYVPRLPKILTNDFQNISVVFGDKTDALGNKDELREVFENTYGLPVVNFIQGSSNVDFTKILNVGIILSG
GPAPGGHNVVAGIFDAIKKSNSNSKIFGFKGGPAGLLDDKKIEITQDLIDAYKNTGGFDIVSSGRTKIETYVQYEQVLSV
VLKNNLNALIIIGGDDSNTNAALLAEFFKKKHHDIQVIGVPKTIDADLRNEHIQISFGFDSATKTYSEMVGNLCRDAMSA
GKYWHFIKIMGRSASHVALECALKTHPNICIISEEVLAKNKTLSEIVEDIASVVIKRSLKGYNFGVIIIPEGVIEFIPEV
KSLMIELCSIFDSNEEEFKGLDVEDIRKVFISKLSEYMKKVYVSLPLFIQIELVNSVLERDPHGNFHVSRVPTEKLFMEM
VSVRLEELRNLGEYSGKFSPIDHFFGYEGRSVTPSNFDSDYCYSLGYNAALLVLNGFTGYMSTIKNLNKNSTEWIAGGVP
ITMMMNMEERYGVLKPVIKKALVDLNGAPFNEFVKHREQWAINNLYVFPGPIQYFGASELVDEITLTLKLELET

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=245, Percent_Identity=25.3061224489796, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 61934; Mature: 61934

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISILQKERYKYVPRLPKILTNDFQNISVVFGDKTDALGNKDELREVFENTYGLPVVNFI
CCCCHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEE
QGSSNVDFTKILNVGIILSGGPAPGGHNVVAGIFDAIKKSNSNSKIFGFKGGPAGLLDDK
CCCCCCCHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
KIEITQDLIDAYKNTGGFDIVSSGRTKIETYVQYEQVLSVVLKNNLNALIIIGGDDSNTN
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
AALLAEFFKKKHHDIQVIGVPKTIDADLRNEHIQISFGFDSATKTYSEMVGNLCRDAMSA
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHC
GKYWHFIKIMGRSASHVALECALKTHPNICIISEEVLAKNKTLSEIVEDIASVVIKRSLK
CCCEEEEEEECCCCCCEEEEEEECCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCC
GYNFGVIIIPEGVIEFIPEVKSLMIELCSIFDSNEEEFKGLDVEDIRKVFISKLSEYMKK
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
VYVSLPLFIQIELVNSVLERDPHGNFHVSRVPTEKLFMEMVSVRLEELRNLGEYSGKFSP
HHHCCCCEEEHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IDHFFGYEGRSVTPSNFDSDYCYSLGYNAALLVLNGFTGYMSTIKNLNKNSTEWIAGGVP
HHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCHHHHHHHHHCCCCCCCEEECCCC
ITMMMNMEERYGVLKPVIKKALVDLNGAPFNEFVKHREQWAINNLYVFPGPIQYFGASEL
EEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHCCHHHH
VDEITLTLKLELET
HHHEEEEEEEEECC
>Mature Secondary Structure
MISILQKERYKYVPRLPKILTNDFQNISVVFGDKTDALGNKDELREVFENTYGLPVVNFI
CCCCHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEE
QGSSNVDFTKILNVGIILSGGPAPGGHNVVAGIFDAIKKSNSNSKIFGFKGGPAGLLDDK
CCCCCCCHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
KIEITQDLIDAYKNTGGFDIVSSGRTKIETYVQYEQVLSVVLKNNLNALIIIGGDDSNTN
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
AALLAEFFKKKHHDIQVIGVPKTIDADLRNEHIQISFGFDSATKTYSEMVGNLCRDAMSA
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHC
GKYWHFIKIMGRSASHVALECALKTHPNICIISEEVLAKNKTLSEIVEDIASVVIKRSLK
CCCEEEEEEECCCCCCEEEEEEECCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCC
GYNFGVIIIPEGVIEFIPEVKSLMIELCSIFDSNEEEFKGLDVEDIRKVFISKLSEYMKK
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
VYVSLPLFIQIELVNSVLERDPHGNFHVSRVPTEKLFMEMVSVRLEELRNLGEYSGKFSP
HHHCCCCEEEHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IDHFFGYEGRSVTPSNFDSDYCYSLGYNAALLVLNGFTGYMSTIKNLNKNSTEWIAGGVP
HHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCHHHHHHHHHCCCCCCCEEECCCC
ITMMMNMEERYGVLKPVIKKALVDLNGAPFNEFVKHREQWAINNLYVFPGPIQYFGASEL
EEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHCCHHHH
VDEITLTLKLELET
HHHEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]