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The map label for this gene is queA [H]

Identifier: 203283951

GI number: 203283951

Start: 32041

End: 33099

Strand: Reverse

Name: queA [H]

Synonym: BDU_27

Alternate gene names: 203283951

Gene position: 33099-32041 (Counterclockwise)

Preceding gene: 203283952

Following gene: 203283950

Centisome position: 3.55

GC content: 29.18

Gene sequence:

>1059_bases
TTGATATTAGTAGGTAGTATGGAGACTAAGGAATTTTATTTTGATTTACCACATCACTTAATAGCACAGTATCCAAGTGA
AAGAAGAGGTTTGGCAAAATTAATGGTTTTGGATTCTATTTGTCAAAAAATTTATCATACTGATTCAGTAAATGATATTT
TAAAGTATATTGGCAGCAATACTTTTTTAGTATTTAATGATTCAAGAGTAAGGAAATCGAGGATATATGCTAAGACAGAT
TATGATGGTAATGTTGAGTTTTTAATTTTAAATAGATTGACAGGAGATACATTTACGTCTTTAATTTCTAAGGCTAAAAG
ACAAAAAGTTGGTAAAGTGTATAGATTTCCTCAGGATTTGTCAGCTCAGATAATTTCAAAATTAGATAATGAATTTACGA
TTAAATTTAATAGATATGTGGATGAATCTTATTTTGAACAGTATGGGTTTATTCCTTTGCCCCCTTATATTAAAAGAGAT
TATGATAAAGAGGATGAAGATCGTTATCAGACTATTTATTCAAAATATATTGGTTCATCAGCATCAGCTACAGCGGGTTT
ACATTTTAGTGAAGAATTATTTTCTAAATTTGCTCAGAATAATATTGAATATGATTTTATTACACTTCATGTTGGACTTG
GGACTTTTCTTCCCGTTAGGACCAAAACAATAGAAAAACATAAAATGCATTTTGAAAGTTTTTCAATAAGAGATTCTGTA
GCTTGTAGGCTTGAGAGGGCTAAGTCTTTAGGTAAAAAAATTTTAGCTGTTGGGACTACTACTCTTAGGGCATTGGAATC
TGCTTATGATAAACAAAAGGGGAGGTTTACTAGAGGTGAACAGAAAACCAATCTTTTTATTTATCCAGGTAAAAATTATA
AATTTAAATCTGTTGATATGCTTTTTACAAATTTTCATACACCAGAATCGACACTTTTAATGTTAGTCTCTTCTTTTGGT
GGTAAAGATTTCGTATTTAATGCTTATAGAGAAGCTGTTACAATGAATTACAGATTTTTTTCTTATGGTGATGCTACTTT
ATTTTTAAGTCATATATAG

Upstream 100 bases:

>100_bases
CTTGTTTTAAGGGGTTTTATTGAGAGAACTAATAGAGGACGCAGAGCTACTGATTTTGCGTATTTGCATTTAAATTTAAA
CAAAAATAATCAAACCATTT

Downstream 100 bases:

>100_bases
ATATTAATGATTAATTATTTTAGTATTTACTTAATAATAATTTAGATAAATCTTGAAAGGAGAAGATATATGATTTCAAT
TTTGCAAAAGGAAAGATATA

Product: S-adenosylmethionine-tRNA ribosyltransferase-isomerase (queuine synthetase)

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MILVGSMETKEFYFDLPHHLIAQYPSERRGLAKLMVLDSICQKIYHTDSVNDILKYIGSNTFLVFNDSRVRKSRIYAKTD
YDGNVEFLILNRLTGDTFTSLISKAKRQKVGKVYRFPQDLSAQIISKLDNEFTIKFNRYVDESYFEQYGFIPLPPYIKRD
YDKEDEDRYQTIYSKYIGSSASATAGLHFSEELFSKFAQNNIEYDFITLHVGLGTFLPVRTKTIEKHKMHFESFSIRDSV
ACRLERAKSLGKKILAVGTTTLRALESAYDKQKGRFTRGEQKTNLFIYPGKNYKFKSVDMLFTNFHTPESTLLMLVSSFG
GKDFVFNAYREAVTMNYRFFSYGDATLFLSHI

Sequences:

>Translated_352_residues
MILVGSMETKEFYFDLPHHLIAQYPSERRGLAKLMVLDSICQKIYHTDSVNDILKYIGSNTFLVFNDSRVRKSRIYAKTD
YDGNVEFLILNRLTGDTFTSLISKAKRQKVGKVYRFPQDLSAQIISKLDNEFTIKFNRYVDESYFEQYGFIPLPPYIKRD
YDKEDEDRYQTIYSKYIGSSASATAGLHFSEELFSKFAQNNIEYDFITLHVGLGTFLPVRTKTIEKHKMHFESFSIRDSV
ACRLERAKSLGKKILAVGTTTLRALESAYDKQKGRFTRGEQKTNLFIYPGKNYKFKSVDMLFTNFHTPESTLLMLVSSFG
GKDFVFNAYREAVTMNYRFFSYGDATLFLSHI
>Mature_352_residues
MILVGSMETKEFYFDLPHHLIAQYPSERRGLAKLMVLDSICQKIYHTDSVNDILKYIGSNTFLVFNDSRVRKSRIYAKTD
YDGNVEFLILNRLTGDTFTSLISKAKRQKVGKVYRFPQDLSAQIISKLDNEFTIKFNRYVDESYFEQYGFIPLPPYIKRD
YDKEDEDRYQTIYSKYIGSSASATAGLHFSEELFSKFAQNNIEYDFITLHVGLGTFLPVRTKTIEKHKMHFESFSIRDSV
ACRLERAKSLGKKILAVGTTTLRALESAYDKQKGRFTRGEQKTNLFIYPGKNYKFKSVDMLFTNFHTPESTLLMLVSSFG
GKDFVFNAYREAVTMNYRFFSYGDATLFLSHI

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=39.8843930635838, Blast_Score=243, Evalue=9e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40852; Mature: 40852

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVGSMETKEFYFDLPHHLIAQYPSERRGLAKLMVLDSICQKIYHTDSVNDILKYIGSN
CEEECCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
TFLVFNDSRVRKSRIYAKTDYDGNVEFLILNRLTGDTFTSLISKAKRQKVGKVYRFPQDL
EEEEECCCCHHHHHEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHH
SAQIISKLDNEFTIKFNRYVDESYFEQYGFIPLPPYIKRDYDKEDEDRYQTIYSKYIGSS
HHHHHHHCCCCEEEEEEHHHCHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCC
ASATAGLHFSEELFSKFAQNNIEYDFITLHVGLGTFLPVRTKTIEKHKMHFESFSIRDSV
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHH
ACRLERAKSLGKKILAVGTTTLRALESAYDKQKGRFTRGEQKTNLFIYPGKNYKFKSVDM
HHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEHEE
LFTNFHTPESTLLMLVSSFGGKDFVFNAYREAVTMNYRFFSYGDATLFLSHI
EEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHEEEEEEEEEECCCEEEEECC
>Mature Secondary Structure
MILVGSMETKEFYFDLPHHLIAQYPSERRGLAKLMVLDSICQKIYHTDSVNDILKYIGSN
CEEECCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
TFLVFNDSRVRKSRIYAKTDYDGNVEFLILNRLTGDTFTSLISKAKRQKVGKVYRFPQDL
EEEEECCCCHHHHHEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHH
SAQIISKLDNEFTIKFNRYVDESYFEQYGFIPLPPYIKRDYDKEDEDRYQTIYSKYIGSS
HHHHHHHCCCCEEEEEEHHHCHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCC
ASATAGLHFSEELFSKFAQNNIEYDFITLHVGLGTFLPVRTKTIEKHKMHFESFSIRDSV
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHH
ACRLERAKSLGKKILAVGTTTLRALESAYDKQKGRFTRGEQKTNLFIYPGKNYKFKSVDM
HHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEHEE
LFTNFHTPESTLLMLVSSFGGKDFVFNAYREAVTMNYRFFSYGDATLFLSHI
EEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHEEEEEEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA