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The map label for this gene is ispG [H]

Identifier: 308535324

GI number: 308535324

Start: 3335590

End: 3336639

Strand: Reverse

Name: ispG [H]

Synonym: NA

Alternate gene names: 308535324

Gene position: 3336639-3335590 (Counterclockwise)

Preceding gene: 197119267

Following gene: 197119263

Centisome position: 72.3

GC content: 61.71

Gene sequence:

>1050_bases
ATGAGAAAAACGACCAGGCAGATCATGATAGGGAATATCCCGGTTGGCGGAGGTGCTCCCTGCTCCGTCCAGTCCATGTG
CTCAACCGACACCCGTGATGTCGCGGCAACCCTTGGGCAGATCGGCCGGCTTGCCGCCGCCGGATGCGAGATCGTGCGCT
GTGCGGTGCCGGATATGGACGCCGCCCTGGCCCTTGCCGCCATAAAGGCCGGCTCCCCCATGCCGCTCATAGCCGACATA
CATTTCGACTACAAGCTTGCCTTGAAGGCCCTGGAGTCCGGGGTGGACGGGCTCCGTCTCAACCCCGGCAACATAGGCGA
AAAGTGGAAGGTTGCCGAGGTGGTGAAAGCCGCGGCCGAGCGCAACGTTCCCATCCGCATCGGCGTCAACGGCGGTTCGC
TGGAAAAGGAACTGCTGGTGAAGTACGGGCACCCGACTCCCGAGGCCATGGTCGAATCGGCGCTGGGGCATGTGCGGATC
CTCGAGGAACTGGGCTATCAGCAGATAAAGATATCGATCAAGGTCTCCGACGTATTGCGGACCCTGGAGGCGTACCGGCT
TCTTTCCGACGCCGTTGACTACCCTCTGCACATAGGCGTTACCGAGGCCGGAACCATCTTCGCCGGAACCGTCAAGTCCT
CCGTAGGTCTGGGAATACTTCTTAACCAGGGGATCGGCGACACCATGCGGGTCTCCCTCACCGGCGATCCGGTTGACGAG
GTGCGGGTAGCGTACGACATACTCAAATCGCTCGGTTTGCGGACCCGCGGCATCAACTTCGTCTCCTGCCCCACCTGTGG
GCGTTGCCAGGTGAACCTGATACCCGTAGCCGAGGAAGTAGAGCGGCGCCTGGCGCATCTGGATACAACGATCACCGTCG
CCGTCATGGGGTGCTCCGTCAACGGACCCGGCGAGGCTCGCGAGGCCGACTTCGGCATAGCCGGGGGTAGGGGAGAAGGG
CTTCTCTTTCGGCACGGCGAGATCCTGCGCAAGGTCCCCGAAGCCGAGCTAGCCGATGCCTTGGTGGAAGAAGTCTTAAA
AAATCAGTAG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:
NA

Sequences:

>Translated_349_residues
MRKTTRQIMIGNIPVGGGAPCSVQSMCSTDTRDVAATLGQIGRLAAAGCEIVRCAVPDMDAALALAAIKAGSPMPLIADI
HFDYKLALKALESGVDGLRLNPGNIGEKWKVAEVVKAAAERNVPIRIGVNGGSLEKELLVKYGHPTPEAMVESALGHVRI
LEELGYQQIKISIKVSDVLRTLEAYRLLSDAVDYPLHIGVTEAGTIFAGTVKSSVGLGILLNQGIGDTMRVSLTGDPVDE
VRVAYDILKSLGLRTRGINFVSCPTCGRCQVNLIPVAEEVERRLAHLDTTITVAVMGCSVNGPGEAREADFGIAGGRGEG
LLFRHGEILRKVPEAELADALVEEVLKNQ
>Mature_349_residues
MRKTTRQIMIGNIPVGGGAPCSVQSMCSTDTRDVAATLGQIGRLAAAGCEIVRCAVPDMDAALALAAIKAGSPMPLIADI
HFDYKLALKALESGVDGLRLNPGNIGEKWKVAEVVKAAAERNVPIRIGVNGGSLEKELLVKYGHPTPEAMVESALGHVRI
LEELGYQQIKISIKVSDVLRTLEAYRLLSDAVDYPLHIGVTEAGTIFAGTVKSSVGLGILLNQGIGDTMRVSLTGDPVDE
VRVAYDILKSLGLRTRGINFVSCPTCGRCQVNLIPVAEEVERRLAHLDTTITVAVMGCSVNGPGEAREADFGIAGGRGEG
LLFRHGEILRKVPEAELADALVEEVLKNQ

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=340, Percent_Identity=51.7647058823529, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37008; Mature: 37008

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTTRQIMIGNIPVGGGAPCSVQSMCSTDTRDVAATLGQIGRLAAAGCEIVRCAVPDMD
CCCCCCEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
AALALAAIKAGSPMPLIADIHFDYKLALKALESGVDGLRLNPGNIGEKWKVAEVVKAAAE
HHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHH
RNVPIRIGVNGGSLEKELLVKYGHPTPEAMVESALGHVRILEELGYQQIKISIKVSDVLR
CCCCEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHH
TLEAYRLLSDAVDYPLHIGVTEAGTIFAGTVKSSVGLGILLNQGIGDTMRVSLTGDPVDE
HHHHHHHHHHHCCCCEEEECCCCCEEEEEEHHCCCCEEEEEECCCCCEEEEEECCCCHHH
VRVAYDILKSLGLRTRGINFVSCPTCGRCQVNLIPVAEEVERRLAHLDTTITVAVMGCSV
HHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHCCEEEEEEEEECC
NGPGEAREADFGIAGGRGEGLLFRHGEILRKVPEAELADALVEEVLKNQ
CCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKTTRQIMIGNIPVGGGAPCSVQSMCSTDTRDVAATLGQIGRLAAAGCEIVRCAVPDMD
CCCCCCEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
AALALAAIKAGSPMPLIADIHFDYKLALKALESGVDGLRLNPGNIGEKWKVAEVVKAAAE
HHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHH
RNVPIRIGVNGGSLEKELLVKYGHPTPEAMVESALGHVRILEELGYQQIKISIKVSDVLR
CCCCEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHH
TLEAYRLLSDAVDYPLHIGVTEAGTIFAGTVKSSVGLGILLNQGIGDTMRVSLTGDPVDE
HHHHHHHHHHHCCCCEEEECCCCCEEEEEEHHCCCCEEEEEECCCCCEEEEEECCCCHHH
VRVAYDILKSLGLRTRGINFVSCPTCGRCQVNLIPVAEEVERRLAHLDTTITVAVMGCSV
HHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHCCEEEEEEEEECC
NGPGEAREADFGIAGGRGEGLLFRHGEILRKVPEAELADALVEEVLKNQ
CCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA