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The map label for this gene is proS

Identifier: 197119263

GI number: 197119263

Start: 3333548

End: 3335269

Strand: Reverse

Name: proS

Synonym: Gbem_2891

Alternate gene names: 197119263

Gene position: 3335269-3333548 (Counterclockwise)

Preceding gene: 308535324

Following gene: 197119262

Centisome position: 72.27

GC content: 61.03

Gene sequence:

>1722_bases
ATGCGTTATTCCCAGTACTTTATTCCGACTGTCAAGGAGACTCCTTCCGATGCGGAAGTCATCTCCCACAAGTTGATGCT
GCGCGCCGGCATGATCAGGAAACTCGCCGCCGGTATCTACAACTACCTCCCGTTCGGCCTTCGCTCTATCCGTAAGGTCG
AGGCCATCGTCCGCGAGGAGATGAACCGCGCCGGCGCCATCGAACTCCTGATGCCTGCCGTCCAGCCTGCCGAACTCTGG
AAGGAGTCGGGGCGCTGGGAATTCTACGGCAAGGAACTCCTTCGCTTCAACGACAGAAAAGACGCCGAGTTCTGCATGGG
CCCCACCCACGAGGAGGTCATCACCGACCTCATCCGCAAGGAAGTCCGCAGCTACCGGCAACTGCCGATCAACCTGTACC
AGATCCAGGGCAAGTTCCGCGACGAGATCCGCCCGCGCTTCGGTCTGATGCGCGGGCGCGAGTTCATCATGAAGGACGCT
TACTCCTTCGACGTGAACGAGGCCGGTGCCGACGTCTCCTACGAGAAAATGTACAAGGCCTACCGCCGCATCTTCGAGCG
CTGCGGGCTTAAGTTCCGCGCCGTCGAAGCCGACACCGGCACCATCGGCGGGAGCTACTCCCACGAATTCATGGTGCTTG
CCGACTCCGGCGAGGACGCCATCGTCTCCTGCTCCGCCTGCGAGTACGCCGCCAACATGGAAAAGGCCGAGACCCGCAAA
GGCGAGGGGATCGAGCATGCCGACCCGCGTCCGATGGAGCACGTGAGCACCCCGGGGCAAAAGAGCATCGAGGATGTGGC
AACGTTCCTCGGTGTGCAGAACACCCAGGTCGTGAAGACGCTGGTGCTGGTCGCCGACGGCGAGCCGGTCGTGGCTCTTA
TCCGCGGCGACTATGACCTGAACGAGATCAAGCTGAAAAATCACCTGGGATGCGCAGAGCTGGAGATGGCCGAGGACGAC
GTGGTCGTCAAGGTCACCGGCGCACCCACCGGCTACGCCGGCCCCGTGGGGCTCGCTGCCAAAGTGAAAGTCGTAGCCGA
CCTCTCCCTGGAGGGGATGCACAACTTCGTCACCGGCGCCAATGCAGCCGACACCCACCTGAAAAACGTGAACATCGGGC
GTGATTTCAGCGTCAGCGGCTTCGTAGACATCAGGAACGTCGTCATCGGCGACGCCTGCCCGCGCTGTGACAGCGGCAAG
CTGGAGATCTGGCGCGGCATCGAAGTCGGTCACGTCTTCAAACTCGGCACCAAGTACTCCAAGGCACTCAAGGCCACCTT
CCTCGATGCCGACGGCAAAGAGCAGATCATCTTCATGGGGTGCTACGGCATCGGCGTCGGGCGCACCGTCGCCGCCTGCA
TCGAGCAGAACCACGACGAGAACGGCATCATCTTCCCGATTCCCATCGCGCCGTTCCAGTGCATCATCTCCTCCTTGAGC
GCCAAAGAGGACGAGGTCAAGGCTGCCTCCGAGTCAATCTACCAGGAACTTCTGGAGGCGGGCATAGAAGTGCTTCTCGA
CGACCGCGACGAGCGCCCCGGCTTCAAGTTCAAGGACGCCGACCTCATCGGGATACCGCTCAGAATAGTCGTAGGCGCCA
AGGCCCTGGCAGAAGGCAAAGTCGAGCTGAAAGAGAGAAGAAGCGGCGAAGTAGAGGTACTCCCCATCGCCGAAGCCATA
GCCAAGGTAAAAGCCGCCGTAAAGGAAGCGCTGCAGGTATAA

Upstream 100 bases:

>100_bases
CTCCGTGCCAATGGTTTTGTTTTCAGGTTTTGGTTTTACCTCAGTGTTCCTCTGTGGTTAATGCTTTTATAGTTTTTTCA
ATTCATAGTGAGGTCGATTC

Downstream 100 bases:

>100_bases
GAAACCAAACCATTGGCAAGGAGCACATCTGAGGACATCTGAGCATAAACAAAGGCTTGAGGTTAACCTCCAAAAGCTTT
TCTCAGAAGTCCTCAGACTG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MRYSQYFIPTVKETPSDAEVISHKLMLRAGMIRKLAAGIYNYLPFGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
KESGRWEFYGKELLRFNDRKDAEFCMGPTHEEVITDLIRKEVRSYRQLPINLYQIQGKFRDEIRPRFGLMRGREFIMKDA
YSFDVNEAGADVSYEKMYKAYRRIFERCGLKFRAVEADTGTIGGSYSHEFMVLADSGEDAIVSCSACEYAANMEKAETRK
GEGIEHADPRPMEHVSTPGQKSIEDVATFLGVQNTQVVKTLVLVADGEPVVALIRGDYDLNEIKLKNHLGCAELEMAEDD
VVVKVTGAPTGYAGPVGLAAKVKVVADLSLEGMHNFVTGANAADTHLKNVNIGRDFSVSGFVDIRNVVIGDACPRCDSGK
LEIWRGIEVGHVFKLGTKYSKALKATFLDADGKEQIIFMGCYGIGVGRTVAACIEQNHDENGIIFPIPIAPFQCIISSLS
AKEDEVKAASESIYQELLEAGIEVLLDDRDERPGFKFKDADLIGIPLRIVVGAKALAEGKVELKERRSGEVEVLPIAEAI
AKVKAAVKEALQV

Sequences:

>Translated_573_residues
MRYSQYFIPTVKETPSDAEVISHKLMLRAGMIRKLAAGIYNYLPFGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
KESGRWEFYGKELLRFNDRKDAEFCMGPTHEEVITDLIRKEVRSYRQLPINLYQIQGKFRDEIRPRFGLMRGREFIMKDA
YSFDVNEAGADVSYEKMYKAYRRIFERCGLKFRAVEADTGTIGGSYSHEFMVLADSGEDAIVSCSACEYAANMEKAETRK
GEGIEHADPRPMEHVSTPGQKSIEDVATFLGVQNTQVVKTLVLVADGEPVVALIRGDYDLNEIKLKNHLGCAELEMAEDD
VVVKVTGAPTGYAGPVGLAAKVKVVADLSLEGMHNFVTGANAADTHLKNVNIGRDFSVSGFVDIRNVVIGDACPRCDSGK
LEIWRGIEVGHVFKLGTKYSKALKATFLDADGKEQIIFMGCYGIGVGRTVAACIEQNHDENGIIFPIPIAPFQCIISSLS
AKEDEVKAASESIYQELLEAGIEVLLDDRDERPGFKFKDADLIGIPLRIVVGAKALAEGKVELKERRSGEVEVLPIAEAI
AKVKAAVKEALQV
>Mature_573_residues
MRYSQYFIPTVKETPSDAEVISHKLMLRAGMIRKLAAGIYNYLPFGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
KESGRWEFYGKELLRFNDRKDAEFCMGPTHEEVITDLIRKEVRSYRQLPINLYQIQGKFRDEIRPRFGLMRGREFIMKDA
YSFDVNEAGADVSYEKMYKAYRRIFERCGLKFRAVEADTGTIGGSYSHEFMVLADSGEDAIVSCSACEYAANMEKAETRK
GEGIEHADPRPMEHVSTPGQKSIEDVATFLGVQNTQVVKTLVLVADGEPVVALIRGDYDLNEIKLKNHLGCAELEMAEDD
VVVKVTGAPTGYAGPVGLAAKVKVVADLSLEGMHNFVTGANAADTHLKNVNIGRDFSVSGFVDIRNVVIGDACPRCDSGK
LEIWRGIEVGHVFKLGTKYSKALKATFLDADGKEQIIFMGCYGIGVGRTVAACIEQNHDENGIIFPIPIAPFQCIISSLS
AKEDEVKAASESIYQELLEAGIEVLLDDRDERPGFKFKDADLIGIPLRIVVGAKALAEGKVELKERRSGEVEVLPIAEAI
AKVKAAVKEALQV

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=46.4601769911504, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=52.787456445993, Blast_Score=577, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI115532348, Length=294, Percent_Identity=34.0136054421769, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=35.9550561797753, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=567, Percent_Identity=31.9223985890653, Blast_Score=276, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24656200, Length=241, Percent_Identity=39.8340248962656, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_GEOBB (B5EIT9)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139690.1
- ProteinModelPortal:   B5EIT9
- SMR:   B5EIT9
- GeneID:   6779744
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2891
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63387; Mature: 63387

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYSQYFIPTVKETPSDAEVISHKLMLRAGMIRKLAAGIYNYLPFGLRSIRKVEAIVREE
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWKESGRWEFYGKELLRFNDRKDAEFCMGPTHEEVITDLIRK
HHCCCCCHHHHCCCCHHHHHHHCCCEEEECHHHHHCCCCCCCHHHCCCCHHHHHHHHHHH
EVRSYRQLPINLYQIQGKFRDEIRPRFGLMRGREFIMKDAYSFDVNEAGADVSYEKMYKA
HHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YRRIFERCGLKFRAVEADTGTIGGSYSHEFMVLADSGEDAIVSCSACEYAANMEKAETRK
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEHHHHHHHCCHHHHHCC
GEGIEHADPRPMEHVSTPGQKSIEDVATFLGVQNTQVVKTLVLVADGEPVVALIRGDYDL
CCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCEEEEEECCCCC
NEIKLKNHLGCAELEMAEDDVVVKVTGAPTGYAGPVGLAAKVKVVADLSLEGMHNFVTGA
CCEECCCCCCCHHEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCHHHHHHHHCCC
NAADTHLKNVNIGRDFSVSGFVDIRNVVIGDACPRCDSGKLEIWRGIEVGHVFKLGTKYS
CCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCEEECCHHHH
KALKATFLDADGKEQIIFMGCYGIGVGRTVAACIEQNHDENGIIFPIPIAPFQCIISSLS
HHHHHHEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHC
AKEDEVKAASESIYQELLEAGIEVLLDDRDERPGFKFKDADLIGIPLRIVVGAKALAEGK
CCHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCEECCCCEECCCCEEEECCHHHHCCC
VELKERRSGEVEVLPIAEAIAKVKAAVKEALQV
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYSQYFIPTVKETPSDAEVISHKLMLRAGMIRKLAAGIYNYLPFGLRSIRKVEAIVREE
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWKESGRWEFYGKELLRFNDRKDAEFCMGPTHEEVITDLIRK
HHCCCCCHHHHCCCCHHHHHHHCCCEEEECHHHHHCCCCCCCHHHCCCCHHHHHHHHHHH
EVRSYRQLPINLYQIQGKFRDEIRPRFGLMRGREFIMKDAYSFDVNEAGADVSYEKMYKA
HHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YRRIFERCGLKFRAVEADTGTIGGSYSHEFMVLADSGEDAIVSCSACEYAANMEKAETRK
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEHHHHHHHCCHHHHHCC
GEGIEHADPRPMEHVSTPGQKSIEDVATFLGVQNTQVVKTLVLVADGEPVVALIRGDYDL
CCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCEEEEEECCCCC
NEIKLKNHLGCAELEMAEDDVVVKVTGAPTGYAGPVGLAAKVKVVADLSLEGMHNFVTGA
CCEECCCCCCCHHEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCHHHHHHHHCCC
NAADTHLKNVNIGRDFSVSGFVDIRNVVIGDACPRCDSGKLEIWRGIEVGHVFKLGTKYS
CCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCEEECCHHHH
KALKATFLDADGKEQIIFMGCYGIGVGRTVAACIEQNHDENGIIFPIPIAPFQCIISSLS
HHHHHHEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHC
AKEDEVKAASESIYQELLEAGIEVLLDDRDERPGFKFKDADLIGIPLRIVVGAKALAEGK
CCHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCEECCCCEECCCCEEEECCHHHHCCC
VELKERRSGEVEVLPIAEAIAKVKAAVKEALQV
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA