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The map label for this gene is radA [H]

Identifier: 197120361

GI number: 197120361

Start: 4538511

End: 4539869

Strand: Direct

Name: radA [H]

Synonym: Gbem_4000

Alternate gene names: 197120361

Gene position: 4538511-4539869 (Clockwise)

Preceding gene: 308535399

Following gene: 197120362

Centisome position: 98.34

GC content: 65.19

Gene sequence:

>1359_bases
ATGGCCAAGGTAAAAGTAAAGACGGGGTTCGTTTGCTCCGATTGCGGCGCGGACTATCCGAAGTGGCAAGGGCACTGCAC
GGCCTGCGACGCCTGGAGCACCATCAAAGAGGTCAGGCTCGGATCCGAGAGAGCCGCGCGGCGCGATGGTTTCGCCGGAG
CCACCTCGTCGGTCACCCTCCTTTCCCAGGTCGAATGCACCGAACAGTCCCGCTTTTCCAGCGGCAGCGAAGAGTTCGAC
CGGGTGCTCGGCGGGGGCTTCGTCCCCGGCTCCGTCATCCTGATCGGGGGGGACCCGGGCGCCGGGAAAAGCACCATCTT
GCTGCAGACCATGTGCTACGCGGCCGCCTCGAAGGAGGTGCTCTACGTCTCGGGCGAGGAATCGCTGCAGCAGATCGCCG
GGCGCGCCAGAAGGCTGCAACTCCCCCTGGACCGGGTGAAGATGCTGGCCGAGAGTTCGGTGGAAGGGGTACTGGAGGCG
GCGAAAAGCGAGCGCCCCGAAGTGGTGGTAATCGACTCGATCCAGGTGATGCAGTCGGCAGCGCTGGAAGCGGCGCCGGG
AGGGGTTTCCCAGGTGCGCGAGTGCGCGGCGGCGCTCACCTGCTACGCCAAGACCTCCGGAGTGGTGCTGATCATGGTCG
GGCACGTCACCAAGGACCAGTCCCTGGCCGGCCCCATGACCCTCTCCCACATGGTCGACACCCAGGTGATGCTCTCCTCC
ACCGAGGACTCGCGCTACCGCTTGATGCGCACCACCAAGAACCGCTTCGGCCCGGTCAACGAATTGGGCGTCTTCGCCAT
GACCGAGGCGGGGCTGCGCGAGGTGAAAAATCCTTCGGCGATCTTCCTCTCCCGCAGCGAGAAGGCCGCGCCCGGCAGCC
TGATCAGCGTCCTTTGGGAAGGGACCAGGCCGCTTCTGGTCGAGATCCAGTCCCTGGTAGTGGGGGCGCAGTACGGCTCG
CCGCGCAGGCTGGGAATAGGGCTCGATCAAAACCGCATCGCCATGATCCTCGCCGTCATCACCAAGCACGGGGGGCTGGT
GCTCTCGGACCAGGACGTCTTCGTGAACGTCGTGGGGGGAATAAGGGTATTGGAAACCAGCGCCGACCTCGCGGTGGCCC
TGGCCATCGTTTCCAGCTTCCGCAACACGGTGCTCCCCCAGGACCTGCTGGTGTTCGGCGAGGTGGGGCTCTCGGGCGAG
ATCCGCCCGGTCTCCAACGGGGAAATGCGGCTCAAGGAAGGGGTGAAACATGGCTTCACCCGCGCCATCGTGCCCAAGGG
AAACGCGCCGAGAAAGGGGATCCCTGGGTTGGAGGTGGTGGCGGTCACTTCGCTCCCCGAGGCGCTTGAGGCAATTTAA

Upstream 100 bases:

>100_bases
AGGGGGCGCAGCCGTAGAATCGCGCCGCTGTTGACACCTCTCGGCAAAAAGCTTAGGATATCGTAGTTTTGCCCCGCCAG
AGACGCGGGGACGGGGTGAT

Downstream 100 bases:

>100_bases
AACTTGCCAATGAGAAGGTAAAAGGAGGCCGTCGCAGCGTCCAAAGCTGTTTTACCCGCGATTTTTTGTCATTCTCCAGG
CAAATTTCACTTTACTAATG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MAKVKVKTGFVCSDCGADYPKWQGHCTACDAWSTIKEVRLGSERAARRDGFAGATSSVTLLSQVECTEQSRFSSGSEEFD
RVLGGGFVPGSVILIGGDPGAGKSTILLQTMCYAAASKEVLYVSGEESLQQIAGRARRLQLPLDRVKMLAESSVEGVLEA
AKSERPEVVVIDSIQVMQSAALEAAPGGVSQVRECAAALTCYAKTSGVVLIMVGHVTKDQSLAGPMTLSHMVDTQVMLSS
TEDSRYRLMRTTKNRFGPVNELGVFAMTEAGLREVKNPSAIFLSRSEKAAPGSLISVLWEGTRPLLVEIQSLVVGAQYGS
PRRLGIGLDQNRIAMILAVITKHGGLVLSDQDVFVNVVGGIRVLETSADLAVALAIVSSFRNTVLPQDLLVFGEVGLSGE
IRPVSNGEMRLKEGVKHGFTRAIVPKGNAPRKGIPGLEVVAVTSLPEALEAI

Sequences:

>Translated_452_residues
MAKVKVKTGFVCSDCGADYPKWQGHCTACDAWSTIKEVRLGSERAARRDGFAGATSSVTLLSQVECTEQSRFSSGSEEFD
RVLGGGFVPGSVILIGGDPGAGKSTILLQTMCYAAASKEVLYVSGEESLQQIAGRARRLQLPLDRVKMLAESSVEGVLEA
AKSERPEVVVIDSIQVMQSAALEAAPGGVSQVRECAAALTCYAKTSGVVLIMVGHVTKDQSLAGPMTLSHMVDTQVMLSS
TEDSRYRLMRTTKNRFGPVNELGVFAMTEAGLREVKNPSAIFLSRSEKAAPGSLISVLWEGTRPLLVEIQSLVVGAQYGS
PRRLGIGLDQNRIAMILAVITKHGGLVLSDQDVFVNVVGGIRVLETSADLAVALAIVSSFRNTVLPQDLLVFGEVGLSGE
IRPVSNGEMRLKEGVKHGFTRAIVPKGNAPRKGIPGLEVVAVTSLPEALEAI
>Mature_451_residues
AKVKVKTGFVCSDCGADYPKWQGHCTACDAWSTIKEVRLGSERAARRDGFAGATSSVTLLSQVECTEQSRFSSGSEEFDR
VLGGGFVPGSVILIGGDPGAGKSTILLQTMCYAAASKEVLYVSGEESLQQIAGRARRLQLPLDRVKMLAESSVEGVLEAA
KSERPEVVVIDSIQVMQSAALEAAPGGVSQVRECAAALTCYAKTSGVVLIMVGHVTKDQSLAGPMTLSHMVDTQVMLSST
EDSRYRLMRTTKNRFGPVNELGVFAMTEAGLREVKNPSAIFLSRSEKAAPGSLISVLWEGTRPLLVEIQSLVVGAQYGSP
RRLGIGLDQNRIAMILAVITKHGGLVLSDQDVFVNVVGGIRVLETSADLAVALAIVSSFRNTVLPQDLLVFGEVGLSGEI
RPVSNGEMRLKEGVKHGFTRAIVPKGNAPRKGIPGLEVVAVTSLPEALEAI

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=455, Percent_Identity=59.5604395604396, Blast_Score=553, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 47943; Mature: 47812

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVKVKTGFVCSDCGADYPKWQGHCTACDAWSTIKEVRLGSERAARRDGFAGATSSVTL
CCEEEEECCEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHH
LSQVECTEQSRFSSGSEEFDRVLGGGFVPGSVILIGGDPGAGKSTILLQTMCYAAASKEV
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCE
LYVSGEESLQQIAGRARRLQLPLDRVKMLAESSVEGVLEAAKSERPEVVVIDSIQVMQSA
EEECCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
ALEAAPGGVSQVRECAAALTCYAKTSGVVLIMVGHVTKDQSLAGPMTLSHMVDTQVMLSS
HHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEC
TEDSRYRLMRTTKNRFGPVNELGVFAMTEAGLREVKNPSAIFLSRSEKAAPGSLISVLWE
CCCHHHHHHHHHHHHCCCHHHHCCCEECHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHC
GTRPLLVEIQSLVVGAQYGSPRRLGIGLDQNRIAMILAVITKHGGLVLSDQDVFVNVVGG
CCCHHHHHHHHHHEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCEEEEEECC
IRVLETSADLAVALAIVSSFRNTVLPQDLLVFGEVGLSGEIRPVSNGEMRLKEGVKHGFT
EEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCEEECCCCCHHHHHHHHHCCC
RAIVPKGNAPRKGIPGLEVVAVTSLPEALEAI
EEEECCCCCCCCCCCCCEEEEECCCCHHHHCC
>Mature Secondary Structure 
AKVKVKTGFVCSDCGADYPKWQGHCTACDAWSTIKEVRLGSERAARRDGFAGATSSVTL
CEEEEECCEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHH
LSQVECTEQSRFSSGSEEFDRVLGGGFVPGSVILIGGDPGAGKSTILLQTMCYAAASKEV
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCE
LYVSGEESLQQIAGRARRLQLPLDRVKMLAESSVEGVLEAAKSERPEVVVIDSIQVMQSA
EEECCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
ALEAAPGGVSQVRECAAALTCYAKTSGVVLIMVGHVTKDQSLAGPMTLSHMVDTQVMLSS
HHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEC
TEDSRYRLMRTTKNRFGPVNELGVFAMTEAGLREVKNPSAIFLSRSEKAAPGSLISVLWE
CCCHHHHHHHHHHHHCCCHHHHCCCEECHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHC
GTRPLLVEIQSLVVGAQYGSPRRLGIGLDQNRIAMILAVITKHGGLVLSDQDVFVNVVGG
CCCHHHHHHHHHHEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCEEEEEECC
IRVLETSADLAVALAIVSSFRNTVLPQDLLVFGEVGLSGEIRPVSNGEMRLKEGVKHGFT
EEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCEEECCCCCHHHHHHHHHCCC
RAIVPKGNAPRKGIPGLEVVAVTSLPEALEAI
EEEECCCCCCCCCCCCCEEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]