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The map label for this gene is amtB [C]

Identifier: 197119497

GI number: 197119497

Start: 3610462

End: 3611682

Strand: Direct

Name: amtB [C]

Synonym: Gbem_3126

Alternate gene names: 197119497

Gene position: 3610462-3611682 (Clockwise)

Preceding gene: 197119484

Following gene: 197119498

Centisome position: 78.23

GC content: 63.55

Gene sequence:

>1221_bases
ATGAACGGGATGTCAGCGACGACAGCGCTTGGCAGCAGCAGCGATGTACTCTTCCTTATGCTCGGAGCGGTCATGGTTTT
CGCCATGCACGCAGGGTTTGCCTTTCTTGAAGTGGGGACGGTACGCCGGAAAAACCAGGTCAACGCCTTCGTCAAGATCC
TCACCGACTGGTCCGTGTCGACCGTGGTCTACTTCCTGATCGGCTTCCCCATCGCCTACGGCATCAGCTTCCTGAAGCCG
GTCAAGCAGATCCTCGGCGACAACCAGGGGTACGACATCACCCACTATTTCTTCCTGCTCTGCTTCGCGGCCTGCATCCC
CGCCATCATCTCCGGCGGCATAGCGGAGCGCGCCAAGTTCTGGCCGCAGGTCATCGCCGGCGCAATCTTCGCCGGGATCA
CCTACCCCGTCTTCGAGTCCCTCATCTGGGGACAGAGCAGCGCCGGGCTCCAGGCGTTCTTCAAGCAGGTCGGTGGCGCC
GAGTTCCACGACTATGCAGGCTCGGTGGTGGTGCACTCCATCGGGGGCTGGCTCGCGCTCCCCGCAGTCATCATCCTCGG
CCCGCGCATGGGGCGCTACCTGAAAGGGAAGTCGCACCCGATCCCGATCAGCAACATCCCCTTCCTGGCCCTGGGTTCCT
GGATCCTCGCCATCGGCTGGTTCGGCTTCAACGTGATGAGCGCCGGCCACCTGGAGAAAATCTCAGGCATCGTCGCCGTC
AACTCGCTCATGGCCATGGTGGGGGGCGTGCTGGCAGCGCTCATCGCCGGGAAGAACGACCCGGGCTTCGTGCACAACGG
CGCGCTCGCCGGGCTCATCGCCGTCTGCGCAGGCTCCGACCTGATGCACCCGCTCTGTGCCTTTGTCACCGGCGCTATCG
CCTCCGTCATCTTCGTCTACGGCTTCCACATCGAGCAGGAGAAGCTCAAGATCGACGACGTGCTCGGCGTCTGGCCGCTG
CACGGTGTCATCGGCTCCTGGGGCGGCATTGCGGCCGGCATCTTCGGCCAGGAAGCGCTGGGCGGAATGGGCGGGGTGAG
CTTAGCCTCCCAGACCCTCGGTAGCCTCGCCGCCATCGTCTTCGCGCTCTGCAACGGCTTCCTGATCTACGGCATCCTCT
CCAAGACTGTCGGCATAAGGCTGGAGCAGGAGGACGAGTTCAGGGGGGCAGACCTCTCCATTCACAGCATCGGCGCCTAT
CCCGAGGAGCATATCCGGTAG

Upstream 100 bases:

>100_bases
GAAGTCTCTGTTTACAGGTAGATACCGGATTGGCAAAGAGCTTGCTTTCTTTGGGCGCTTCCCCAACGAAGGGGGGGCAT
TTACCAACGGAGGTAGCTAG

Downstream 100 bases:

>100_bases
TTGCCCGGCATAATTGGCAAAAAAAAGGGGTTCCGGTATCGGAACCCCTTTTTTTATTTTGAATTTTGCAGGGAAGAATG
TATTATCTCCACTGTTAATA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNGMSATTALGSSSDVLFLMLGAVMVFAMHAGFAFLEVGTVRRKNQVNAFVKILTDWSVSTVVYFLIGFPIAYGISFLKP
VKQILGDNQGYDITHYFFLLCFAACIPAIISGGIAERAKFWPQVIAGAIFAGITYPVFESLIWGQSSAGLQAFFKQVGGA
EFHDYAGSVVVHSIGGWLALPAVIILGPRMGRYLKGKSHPIPISNIPFLALGSWILAIGWFGFNVMSAGHLEKISGIVAV
NSLMAMVGGVLAALIAGKNDPGFVHNGALAGLIAVCAGSDLMHPLCAFVTGAIASVIFVYGFHIEQEKLKIDDVLGVWPL
HGVIGSWGGIAAGIFGQEALGGMGGVSLASQTLGSLAAIVFALCNGFLIYGILSKTVGIRLEQEDEFRGADLSIHSIGAY
PEEHIR

Sequences:

>Translated_406_residues
MNGMSATTALGSSSDVLFLMLGAVMVFAMHAGFAFLEVGTVRRKNQVNAFVKILTDWSVSTVVYFLIGFPIAYGISFLKP
VKQILGDNQGYDITHYFFLLCFAACIPAIISGGIAERAKFWPQVIAGAIFAGITYPVFESLIWGQSSAGLQAFFKQVGGA
EFHDYAGSVVVHSIGGWLALPAVIILGPRMGRYLKGKSHPIPISNIPFLALGSWILAIGWFGFNVMSAGHLEKISGIVAV
NSLMAMVGGVLAALIAGKNDPGFVHNGALAGLIAVCAGSDLMHPLCAFVTGAIASVIFVYGFHIEQEKLKIDDVLGVWPL
HGVIGSWGGIAAGIFGQEALGGMGGVSLASQTLGSLAAIVFALCNGFLIYGILSKTVGIRLEQEDEFRGADLSIHSIGAY
PEEHIR
>Mature_406_residues
MNGMSATTALGSSSDVLFLMLGAVMVFAMHAGFAFLEVGTVRRKNQVNAFVKILTDWSVSTVVYFLIGFPIAYGISFLKP
VKQILGDNQGYDITHYFFLLCFAACIPAIISGGIAERAKFWPQVIAGAIFAGITYPVFESLIWGQSSAGLQAFFKQVGGA
EFHDYAGSVVVHSIGGWLALPAVIILGPRMGRYLKGKSHPIPISNIPFLALGSWILAIGWFGFNVMSAGHLEKISGIVAV
NSLMAMVGGVLAALIAGKNDPGFVHNGALAGLIAVCAGSDLMHPLCAFVTGAIASVIFVYGFHIEQEKLKIDDVLGVWPL
HGVIGSWGGIAAGIFGQEALGGMGGVSLASQTLGSLAAIVFALCNGFLIYGILSKTVGIRLEQEDEFRGADLSIHSIGAY
PEEHIR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=418, Percent_Identity=28.2296650717703, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=429, Percent_Identity=30.0699300699301, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17550182, Length=427, Percent_Identity=29.5081967213115, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=28.0285035629454, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI133931100, Length=435, Percent_Identity=26.4367816091954, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6325396, Length=364, Percent_Identity=24.7252747252747, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321559, Length=441, Percent_Identity=23.8095238095238, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324187, Length=449, Percent_Identity=23.6080178173719, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=29.6650717703349, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42859; Mature: 42859

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCEECCCCCCCCCCEEEEECCCCCHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCEECCCCCCCCCCEEEEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]