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The map label for this gene is frzE [H]

Identifier: 197119498

GI number: 197119498

Start: 3611816

End: 3612667

Strand: Direct

Name: frzE [H]

Synonym: Gbem_3127

Alternate gene names: 197119498

Gene position: 3611816-3612667 (Clockwise)

Preceding gene: 197119497

Following gene: 197119499

Centisome position: 78.26

GC content: 59.74

Gene sequence:

>852_bases
ATGAAGATACCCTTGGGACAGATCCTGGTACAGTCCGGCATCATCACGGTAAAGACACTGGAACGTGCCCTGACCCGGCA
AGAGGGGTCCGGCAAGCGGCTGGGGGCTATTCTCGATGAGATGGGGGTGATCACCCCGGAAGAACTGGTCGAGGCCCTGG
CCCAGCAGTCGGGGATGGAGATGGTGAAGAGGATCGCTGTGCAAAACGTTCCCGGCGAGCTCCTAGAGCTGGTTCCGGGC
GAAGTCGCCATCAACAAGCTGGTCTTTCCGCTGAAGCGGCAGGGGGGGGTCCTCGCCATCGCCGTCAGCGACCCCATCGA
CAGCGAGACCCTCGACCTCTTGGAACGGCATAGCTGCAACAAAATCGTGCAGGTGCTGGCCGCCCGCGAGGAGATCCTGG
GCGCGATCAAGCAGCACTACCTTCGTAACCAGGCAGCGGATAACGTTTCCTTGAAGATCCTCCTGGTCGACGATAGCGCC
GGCATAAGCTGCGACGTCGAGGGTGCCCTAAAAAACGAAGGATACCAAGTATATACTGCCCGGGACGGGGTCGAAGGGCT
GAAGATAGCCTTCTCTCAACGGCCGGACCTGATCCTTTGCGACGCCGGGGCGCCCAAGATGGACGGCTACGCCCTGATGC
GCGCCATCAAGGCAAACCCTGCCAGCGCCGGGACCCCCATGATACTTTTGACCTCGAAAGCCTCGCCTGAAGAAGAACAC
CGTGCGCTCAAAGCCGGCTTTCACGACTTCATAGCCAAGCCCATGATGACTATCCGCGTAGTCTCGCGCGTGAAACGCGC
ATTCCAGATTTTGGACAAGGGGAAAGAGCAGCAGGCAGTTGCTACGCTGTGA

Upstream 100 bases:

>100_bases
CGGTATCGGAACCCCTTTTTTTATTTTGAATTTTGCAGGGAAGAATGTATTATCTCCACTGTTAATAAATTCTAATAACA
TTTATGAGGCGTACTCTATC

Downstream 100 bases:

>100_bases
CCTCTCCCCACGGTGCCTCGGCTCTTTTCGCTAAACAGCCGAATGCCGGACGCCTTGCCGCTTCCCTTTCCAACCGCAAC
AGCTAAGTTTTGGCGGTGGC

Product: GspIIEN domain-containing response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKIPLGQILVQSGIITVKTLERALTRQEGSGKRLGAILDEMGVITPEELVEALAQQSGMEMVKRIAVQNVPGELLELVPG
EVAINKLVFPLKRQGGVLAIAVSDPIDSETLDLLERHSCNKIVQVLAAREEILGAIKQHYLRNQAADNVSLKILLVDDSA
GISCDVEGALKNEGYQVYTARDGVEGLKIAFSQRPDLILCDAGAPKMDGYALMRAIKANPASAGTPMILLTSKASPEEEH
RALKAGFHDFIAKPMMTIRVVSRVKRAFQILDKGKEQQAVATL

Sequences:

>Translated_283_residues
MKIPLGQILVQSGIITVKTLERALTRQEGSGKRLGAILDEMGVITPEELVEALAQQSGMEMVKRIAVQNVPGELLELVPG
EVAINKLVFPLKRQGGVLAIAVSDPIDSETLDLLERHSCNKIVQVLAAREEILGAIKQHYLRNQAADNVSLKILLVDDSA
GISCDVEGALKNEGYQVYTARDGVEGLKIAFSQRPDLILCDAGAPKMDGYALMRAIKANPASAGTPMILLTSKASPEEEH
RALKAGFHDFIAKPMMTIRVVSRVKRAFQILDKGKEQQAVATL
>Mature_283_residues
MKIPLGQILVQSGIITVKTLERALTRQEGSGKRLGAILDEMGVITPEELVEALAQQSGMEMVKRIAVQNVPGELLELVPG
EVAINKLVFPLKRQGGVLAIAVSDPIDSETLDLLERHSCNKIVQVLAAREEILGAIKQHYLRNQAADNVSLKILLVDDSA
GISCDVEGALKNEGYQVYTARDGVEGLKIAFSQRPDLILCDAGAPKMDGYALMRAIKANPASAGTPMILLTSKASPEEEH
RALKAGFHDFIAKPMMTIRVVSRVKRAFQILDKGKEQQAVATL

Specific function: FrzE is involved in a sensory transduction pathway that controls the frequency at which cells reverse their gliding direction. FrzE seems to be capable of autophosphorylating itself on an histidine residue and then to transfer that group to an aspartate r

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786781, Length=144, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790437, Length=110, Percent_Identity=37.2727272727273, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082012, Length=115, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693157, Length=113, Percent_Identity=35.3982300884956, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787375, Length=115, Percent_Identity=28.695652173913, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR002545
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR005467
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01584 CheW; PF02518 HATPase_c; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 30625; Mature: 30625

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPLGQILVQSGIITVKTLERALTRQEGSGKRLGAILDEMGVITPEELVEALAQQSGME
CCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MVKRIAVQNVPGELLELVPGEVAINKLVFPLKRQGGVLAIAVSDPIDSETLDLLERHSCN
HHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
KIVQVLAAREEILGAIKQHYLRNQAADNVSLKILLVDDSAGISCDVEGALKNEGYQVYTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCHHCCCCEEEEEE
RDGVEGLKIAFSQRPDLILCDAGAPKMDGYALMRAIKANPASAGTPMILLTSKASPEEEH
CCCCCHHHEEECCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHH
RALKAGFHDFIAKPMMTIRVVSRVKRAFQILDKGKEQQAVATL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MKIPLGQILVQSGIITVKTLERALTRQEGSGKRLGAILDEMGVITPEELVEALAQQSGME
CCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MVKRIAVQNVPGELLELVPGEVAINKLVFPLKRQGGVLAIAVSDPIDSETLDLLERHSCN
HHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
KIVQVLAAREEILGAIKQHYLRNQAADNVSLKILLVDDSAGISCDVEGALKNEGYQVYTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCHHCCCCEEEEEE
RDGVEGLKIAFSQRPDLILCDAGAPKMDGYALMRAIKANPASAGTPMILLTSKASPEEEH
CCCCCHHHEEECCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHH
RALKAGFHDFIAKPMMTIRVVSRVKRAFQILDKGKEQQAVATL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2165608; 2123853 [H]