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The map label for this gene is cca [H]

Identifier: 197119266

GI number: 197119266

Start: 3338579

End: 3339778

Strand: Direct

Name: cca [H]

Synonym: Gbem_2894

Alternate gene names: 197119266

Gene position: 3338579-3339778 (Clockwise)

Preceding gene: 197119265

Following gene: 197119282

Centisome position: 72.34

GC content: 62.42

Gene sequence:

>1200_bases
ATGGATGCTTTGATTCTACGACTGAAGAAGTTCTTCCCTGCGTCCCTGCACTCCCGTATCTTCCTCACCGGCGGCATGGT
GCGCGACTTCCTGCTGGGGGTCGCCTGCCAGGACGTCGATCTTGCCGCCGCGGTTACCGCGGCGGAACTCGCCTCTCTCG
GTTTCCGCCTGGTCGAGTCCAAGAGCACCCCCAACATCTACTTCAGTTATCGTCAGGAATTCGGAAAGATCGAGGTCACC
CGCCTGGAGTCGGTGGAGGAACTGACCTCCGATCTTTTCCGCCGCGATTTTCGGGTGAACGCCATGGCGATGAGCCTGGA
CGGCGCGCTCATCGACCCGCTTCACGGGAAGGCCGACCTGGAACAGCGCATCCTGAGCGCCTGCACCCAGACCAGCATCA
CCGACGACCCCTTGAGGATCTTCCGCGCTTTGCGCTTCGAATGCGAGGGGTGGCACCTGGACCGCGAGGCGGAGGCGCTC
TTGGTATCGCTCGACTGGTCCGACGCGTTCGCAGCGATTCCCGTGGAGCGCTTCTCACAGGAGATGCTCAAGGCTTTGGC
GAAGCCCGAGCCCGCTCATTTCTTCAGGCGCATGGTGGAGTTTGAGGCGGGGAGGAACTATCTGCCGGAGATCTTCGAAA
TGCAGCAGGTCATTGCCGGGCCGCCGCAGCACCACCCCGAGGGGGATCTGTTCACCCACTCGATGCAGACGCTGGAGCGG
ATGGCTGCCCTGACCGATGACGTGACCGGCCGGTTCTGCGCGCTCTTCCATGATCTGGGAAAGCTTTATACCCCCAAAGA
CCAGCACCCGAAGCACCACGGCCACGATGCGCTCGGGGCCTCAGCTGCCGATGCTTTCTGCAAGAGGCTGCGCCTGCCGT
CGGCGCTGCAGCGCGCGCTGAAAGCGACCAGCAAGCTGCATGACACTGCGAACAAGTGGGAAGAGTTGAGGGACTCGACC
AAGATCAGGCTGGCCGTCGACGCCGTCAAGGGGGGGATAGCGGAATTTCTGCCGCTTCAGGTTGCGGCGGACTGCGGCGG
AAAGATGCCGGGGTGGGATGAGGCGCTCAGGGTGGCGGCGCTGAACGCCGCGCAGCTGGGGATCGGGCGGGAGATGCTGG
ATAACAAAGAAGTCCCGCCGGAGAAGCTGCAGCAGATAATCATGCAGCTACGGGTGGAGGAGTTGAGAAGAAGTAGATGA

Upstream 100 bases:

>100_bases
CCGGTGGACATGTTCCCGCAGACGGTGCATGTCGAGGACGTGGCGCTTCTGGAAAAAAAGCAGTGACAACAGCGGCAGTA
ATGCCGCTTCTAGGGCTGCC

Downstream 100 bases:

>100_bases
TTGGAATCTTCCCTCACCCTGTCCCTCTCCCAAAGGGCGAGGGGACGTAAGGGGTGGGTCCCTTCCCAAAGTCACCGGGG
AATTGCGTGGATAACGCCCC

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MDALILRLKKFFPASLHSRIFLTGGMVRDFLLGVACQDVDLAAAVTAAELASLGFRLVESKSTPNIYFSYRQEFGKIEVT
RLESVEELTSDLFRRDFRVNAMAMSLDGALIDPLHGKADLEQRILSACTQTSITDDPLRIFRALRFECEGWHLDREAEAL
LVSLDWSDAFAAIPVERFSQEMLKALAKPEPAHFFRRMVEFEAGRNYLPEIFEMQQVIAGPPQHHPEGDLFTHSMQTLER
MAALTDDVTGRFCALFHDLGKLYTPKDQHPKHHGHDALGASAADAFCKRLRLPSALQRALKATSKLHDTANKWEELRDST
KIRLAVDAVKGGIAEFLPLQVAADCGGKMPGWDEALRVAALNAAQLGIGREMLDNKEVPPEKLQQIIMQLRVEELRRSR

Sequences:

>Translated_399_residues
MDALILRLKKFFPASLHSRIFLTGGMVRDFLLGVACQDVDLAAAVTAAELASLGFRLVESKSTPNIYFSYRQEFGKIEVT
RLESVEELTSDLFRRDFRVNAMAMSLDGALIDPLHGKADLEQRILSACTQTSITDDPLRIFRALRFECEGWHLDREAEAL
LVSLDWSDAFAAIPVERFSQEMLKALAKPEPAHFFRRMVEFEAGRNYLPEIFEMQQVIAGPPQHHPEGDLFTHSMQTLER
MAALTDDVTGRFCALFHDLGKLYTPKDQHPKHHGHDALGASAADAFCKRLRLPSALQRALKATSKLHDTANKWEELRDST
KIRLAVDAVKGGIAEFLPLQVAADCGGKMPGWDEALRVAALNAAQLGIGREMLDNKEVPPEKLQQIIMQLRVEELRRSR
>Mature_399_residues
MDALILRLKKFFPASLHSRIFLTGGMVRDFLLGVACQDVDLAAAVTAAELASLGFRLVESKSTPNIYFSYRQEFGKIEVT
RLESVEELTSDLFRRDFRVNAMAMSLDGALIDPLHGKADLEQRILSACTQTSITDDPLRIFRALRFECEGWHLDREAEAL
LVSLDWSDAFAAIPVERFSQEMLKALAKPEPAHFFRRMVEFEAGRNYLPEIFEMQQVIAGPPQHHPEGDLFTHSMQTLER
MAALTDDVTGRFCALFHDLGKLYTPKDQHPKHHGHDALGASAADAFCKRLRLPSALQRALKATSKLHDTANKWEELRDST
KIRLAVDAVKGGIAEFLPLQVAADCGGKMPGWDEALRVAALNAAQLGIGREMLDNKEVPPEKLQQIIMQLRVEELRRSR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=350, Percent_Identity=30.8571428571429, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 44791; Mature: 44791

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALILRLKKFFPASLHSRIFLTGGMVRDFLLGVACQDVDLAAAVTAAELASLGFRLVES
CCHHHHHHHHHCCHHHHCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
KSTPNIYFSYRQEFGKIEVTRLESVEELTSDLFRRDFRVNAMAMSLDGALIDPLHGKADL
CCCCCEEEHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHH
EQRILSACTQTSITDDPLRIFRALRFECEGWHLDREAEALLVSLDWSDAFAAIPVERFSQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHCCHHHHHH
EMLKALAKPEPAHFFRRMVEFEAGRNYLPEIFEMQQVIAGPPQHHPEGDLFTHSMQTLER
HHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MAALTDDVTGRFCALFHDLGKLYTPKDQHPKHHGHDALGASAADAFCKRLRLPSALQRAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
KATSKLHDTANKWEELRDSTKIRLAVDAVKGGIAEFLPLQVAADCGGKMPGWDEALRVAA
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LNAAQLGIGREMLDNKEVPPEKLQQIIMQLRVEELRRSR
HHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDALILRLKKFFPASLHSRIFLTGGMVRDFLLGVACQDVDLAAAVTAAELASLGFRLVES
CCHHHHHHHHHCCHHHHCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
KSTPNIYFSYRQEFGKIEVTRLESVEELTSDLFRRDFRVNAMAMSLDGALIDPLHGKADL
CCCCCEEEHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHH
EQRILSACTQTSITDDPLRIFRALRFECEGWHLDREAEALLVSLDWSDAFAAIPVERFSQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHCCHHHHHH
EMLKALAKPEPAHFFRRMVEFEAGRNYLPEIFEMQQVIAGPPQHHPEGDLFTHSMQTLER
HHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MAALTDDVTGRFCALFHDLGKLYTPKDQHPKHHGHDALGASAADAFCKRLRLPSALQRAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
KATSKLHDTANKWEELRDSTKIRLAVDAVKGGIAEFLPLQVAADCGGKMPGWDEALRVAA
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LNAAQLGIGREMLDNKEVPPEKLQQIIMQLRVEELRRSR
HHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA