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The map label for this gene is ybaK [H]

Identifier: 197119182

GI number: 197119182

Start: 3238830

End: 3239312

Strand: Reverse

Name: ybaK [H]

Synonym: Gbem_2809

Alternate gene names: 197119182

Gene position: 3239312-3238830 (Counterclockwise)

Preceding gene: 197119192

Following gene: 197119181

Centisome position: 70.19

GC content: 62.94

Gene sequence:

>483_bases
ATGGCCAAGTTTAAGTCCCCCGTGACAGCTGCGGTACGCGTGCTGCGGCAGGCAGGGGTATCCTACGGCGAACATCCCTA
CCAGTACGAGGAAAAGGGGGGGACCGCAGTCTCGGCGCGGGAGCTCGGCGTAGACGAGAGCTGCGTCATCAAGACCCTGA
TCATGGAGGACGAGGCGAAAAGCCCCCTCGTGGTCCTCATGCACGGAGACCTCCAGGTCTCCACCAAGGAACTGGCGCGC
GTAATCGGCGTCAAGTCCGTCGCCCCCTGCACCCCGGATACGGCAAACCGCCACTCCGGCTACATGGTGGGGGGGACCTC
ACCCTTCGGCACCAGGAAACAGATGCCGGTCTACCTGGAAGAGAGCATCCTGGAACTGCCGCTCATCTACATTAACGGCG
GCAGCCGCGGTTTCCTGGTTTCCATGCCCCCCTCCGAGCTGGTGCGGGTGCTGCAGCCGGTACTGGTGAAAGTCGGTATC
TAG

Upstream 100 bases:

>100_bases
TAAGCTGCGCGTGGCGTGATATTAATCTATAGCCGGTATGTTTGCAGCTGGCAAGTCACTTTTTCATGTGAGACTCGATT
CATCTTAAGGAGAGCTGGAA

Downstream 100 bases:

>100_bases
GAGGAGCGATGGATCTCTCCAGGAGTTTCGAACAGGCGGCGGAACTGGTGGCGCAGGCCAAGGCGCTGGTCGTGACCGCC
GGAGCTGGCATGGGGGTGGA

Product: misacylated tRNA(Pro) deacylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAKFKSPVTAAVRVLRQAGVSYGEHPYQYEEKGGTAVSARELGVDESCVIKTLIMEDEAKSPLVVLMHGDLQVSTKELAR
VIGVKSVAPCTPDTANRHSGYMVGGTSPFGTRKQMPVYLEESILELPLIYINGGSRGFLVSMPPSELVRVLQPVLVKVGI

Sequences:

>Translated_160_residues
MAKFKSPVTAAVRVLRQAGVSYGEHPYQYEEKGGTAVSARELGVDESCVIKTLIMEDEAKSPLVVLMHGDLQVSTKELAR
VIGVKSVAPCTPDTANRHSGYMVGGTSPFGTRKQMPVYLEESILELPLIYINGGSRGFLVSMPPSELVRVLQPVLVKVGI
>Mature_159_residues
AKFKSPVTAAVRVLRQAGVSYGEHPYQYEEKGGTAVSARELGVDESCVIKTLIMEDEAKSPLVVLMHGDLQVSTKELARV
IGVKSVAPCTPDTANRHSGYMVGGTSPFGTRKQMPVYLEESILELPLIYINGGSRGFLVSMPPSELVRVLQPVLVKVGI

Specific function: Functions in trans to edit the amino acid from incorrectly charged Cys-tRNA(Pro); has much weaker activity against Ala-tRNA(Pro). Probably compensates for the lack of Cys- tRNA(Pro) editing by ProRS. Additionally serves as a general deacylase with weak ac

COG id: COG2606

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prolyl-tRNA editing family. ProX subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786688, Length=160, Percent_Identity=29.375, Blast_Score=78, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004369
- InterPro:   IPR007214 [H]

Pfam domain/function: PF04073 YbaK [H]

EC number: NA

Molecular weight: Translated: 17272; Mature: 17141

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFKSPVTAAVRVLRQAGVSYGEHPYQYEEKGGTAVSARELGVDESCVIKTLIMEDEAK
CCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEHHHCCCCHHHHHHHHHHCCCCC
SPLVVLMHGDLQVSTKELARVIGVKSVAPCTPDTANRHSGYMVGGTSPFGTRKQMPVYLE
CCEEEEEECCCEECHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHH
ESILELPLIYINGGSRGFLVSMPPSELVRVLQPVLVKVGI
HHHHHCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKFKSPVTAAVRVLRQAGVSYGEHPYQYEEKGGTAVSARELGVDESCVIKTLIMEDEAK
CCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEHHHCCCCHHHHHHHHHHCCCCC
SPLVVLMHGDLQVSTKELARVIGVKSVAPCTPDTANRHSGYMVGGTSPFGTRKQMPVYLE
CCEEEEEECCCEECHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHH
ESILELPLIYINGGSRGFLVSMPPSELVRVLQPVLVKVGI
HHHHHCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10813833 [H]