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The map label for this gene is mcrA [H]

Identifier: 197119165

GI number: 197119165

Start: 3219612

End: 3220313

Strand: Reverse

Name: mcrA [H]

Synonym: Gbem_2792

Alternate gene names: 197119165

Gene position: 3220313-3219612 (Counterclockwise)

Preceding gene: 197119177

Following gene: 308535320

Centisome position: 69.78

GC content: 48.01

Gene sequence:

>702_bases
ATGAACTCTAAATTTTCGCAACTTGTGGAATTCGCTGAGCAGCATCAAGGGCGGCCGCTTTCGACTCTTGGGAACAGAAG
CTCGTTCCTGCTGGAGGTGTCAGAATCCTCGTTTACATATACTCCTGTTACGACACAACGGCCCCGTCGCCAAAAGTTAA
TCCATGCGGGGCGGGTTTTTGAGCGTTACCTCGAGATCGGATCATTGAAGACCTCTAAGTACACAGACCTCACGGTGAAC
TCTTCATACATACTGGCCCTGATCAAGGCGTTCTCAGAGAAAGAACCGACTATGAACGAAGAGAAGGTGCACGAATTTGC
ATCTAATGCGATTAATGATTTAGACGAAATCCCGCAGGGGGTGGCTGAGCCTGAAAGGAGTTCTCATACTACATCTTCAT
ACGCAAGGGACCATAAGGTAAGGGCTTATGTGATAGCGCGAGCAAAGGGTACCTGTGAGTATTGCGGTGAACTTGGTTTT
CTGATGTCTAATGGCAAGAGCTACTACCTGGAGGCACATCATGTTATCGCCCTTGCTGACGAAGGTAGGGATACCCCCGA
TAACGTAATTGCTCTTTGCCCAAAGCACCACAAGGAAGCACACTTCGGAATCAATCGTGATGAAATTGAAGAGGAAATGA
TGATCTTTTTAAGCACTTTGTCTAAAAAGGCGCGGAACAATAGGGCTGCACGCAGGCGCTGA

Upstream 100 bases:

>100_bases
TCTTAGCTGACATAGGCGTCATAATGGCGCATGAGGTATGGAAAAGGGCGAATCATGACAATTTCCCCTCAATAACGATC
TGCATATGGAGGGGGATGCT

Downstream 100 bases:

>100_bases
CTCGCTGGTGCGGCCCGCACTCGTTATACTAGTAAGTACGAGGAGACTATGAAAAGGTGGATGACGTATATGCTTTTGGC
CGTATCAATCTCGGGCTGTG

Product: HNH endonuclease

Products: NA

Alternate protein names: EcoKMcrA [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MNSKFSQLVEFAEQHQGRPLSTLGNRSSFLLEVSESSFTYTPVTTQRPRRQKLIHAGRVFERYLEIGSLKTSKYTDLTVN
SSYILALIKAFSEKEPTMNEEKVHEFASNAINDLDEIPQGVAEPERSSHTTSSYARDHKVRAYVIARAKGTCEYCGELGF
LMSNGKSYYLEAHHVIALADEGRDTPDNVIALCPKHHKEAHFGINRDEIEEEMMIFLSTLSKKARNNRAARRR

Sequences:

>Translated_233_residues
MNSKFSQLVEFAEQHQGRPLSTLGNRSSFLLEVSESSFTYTPVTTQRPRRQKLIHAGRVFERYLEIGSLKTSKYTDLTVN
SSYILALIKAFSEKEPTMNEEKVHEFASNAINDLDEIPQGVAEPERSSHTTSSYARDHKVRAYVIARAKGTCEYCGELGF
LMSNGKSYYLEAHHVIALADEGRDTPDNVIALCPKHHKEAHFGINRDEIEEEMMIFLSTLSKKARNNRAARRR
>Mature_233_residues
MNSKFSQLVEFAEQHQGRPLSTLGNRSSFLLEVSESSFTYTPVTTQRPRRQKLIHAGRVFERYLEIGSLKTSKYTDLTVN
SSYILALIKAFSEKEPTMNEEKVHEFASNAINDLDEIPQGVAEPERSSHTTSSYARDHKVRAYVIARAKGTCEYCGELGF
LMSNGKSYYLEAHHVIALADEGRDTPDNVIALCPKHHKEAHFGINRDEIEEEMMIFLSTLSKKARNNRAARRR

Specific function: Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG [H]

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787406, Length=128, Percent_Identity=35.15625, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002711
- InterPro:   IPR003615 [H]

Pfam domain/function: PF01844 HNH [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 26500; Mature: 26500

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKFSQLVEFAEQHQGRPLSTLGNRSSFLLEVSESSFTYTPVTTQRPRRQKLIHAGRVF
CCCHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHH
ERYLEIGSLKTSKYTDLTVNSSYILALIKAFSEKEPTMNEEKVHEFASNAINDLDEIPQG
HHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VAEPERSSHTTSSYARDHKVRAYVIARAKGTCEYCGELGFLMSNGKSYYLEAHHVIALAD
HCCCCCCCCCHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEEEEECC
EGRDTPDNVIALCPKHHKEAHFGINRDEIEEEMMIFLSTLSKKARNNRAARRR
CCCCCCCCEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCC
>Mature Secondary Structure
MNSKFSQLVEFAEQHQGRPLSTLGNRSSFLLEVSESSFTYTPVTTQRPRRQKLIHAGRVF
CCCHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHH
ERYLEIGSLKTSKYTDLTVNSSYILALIKAFSEKEPTMNEEKVHEFASNAINDLDEIPQG
HHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VAEPERSSHTTSSYARDHKVRAYVIARAKGTCEYCGELGFLMSNGKSYYLEAHHVIALAD
HCCCCCCCCCHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEEEEECC
EGRDTPDNVIALCPKHHKEAHFGINRDEIEEEMMIFLSTLSKKARNNRAARRR
CCCCCCCCEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1938927; 8905232; 9278503 [H]