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The map label for this gene is ispE

Identifier: 197119148

GI number: 197119148

Start: 3200515

End: 3201357

Strand: Reverse

Name: ispE

Synonym: Gbem_2775

Alternate gene names: 197119148

Gene position: 3201357-3200515 (Counterclockwise)

Preceding gene: 197119149

Following gene: 197119147

Centisome position: 69.37

GC content: 60.02

Gene sequence:

>843_bases
GTGAAAAAACTGCAACTGCTGGCGCCGGCCAAGGTCAACTACCGGCTGGACGTATTGGGCAAGAGGCCGGACGGCTATCA
CGAGCTGCGCATGATCATGCAGAGGGTCGACCTCTGCGACGAGATAGAGATTGCCCTTTCCGATTCCCCCGGGATCCGGG
TTACCTGCGGCAGGAAAGGGGTGCCCGACGGGCCGGGAAACATAGCCTGGCGTGCAGCCGACGCACTGTTGAAGCTTTCG
GACAAGGAAGTCGGCATAGAGATCACCATTGCCAAGAAGATACCGGTAGGGGCAGGGCTCGGGGGGGGGAGCAGCGACGC
CGCCACTGTCCTGATGGGAGTGAACGAGCTCCTCGAATTGGGTCTTACGGACGAGCGGTTGATGGAGATCGGCGTCAAGC
TGGGTGCCGACGTTCCGTTCTTCGTCTTCAAAAAGCCCGCCCTTGCCGAGGGGATCGGCGACCGCCTCACCGCGCTGGAA
GAAGTTCCCTCCCTCTGGGTGGTGCTGGTGAATCCCGGGATACATGTTTCCACTGCCTGGGTGTATCAAAATTTAAGGTT
GACAACGCCGGATCCCGCCACTATAATCCCGCGTTCATACAGCAGCCTCAACGAGGTCTGCGAGCTCCTTTCCAACGATT
TGGAGCCGGTCACCTGCGGGAGATTCCCCCTGGTGAGCGAACTGAAAGAGGTGCTGTTGACAGCAGGTGCCCGCGGGTCG
CTGATGTCAGGAAGCGGCTCCACCGTTTTCGGTCTTTTCGAGAGCGAAAGCGCGGCGCGACATGCGGCAGCTGAGATAGC
AAAAGCGCGTGGCTGGTTCGCAGCCGCCGTCAGGACCATTTAA

Upstream 100 bases:

>100_bases
GGGAGACTGTGCTGCCGAGCTTTTTCCAGCTCTCCCCCTCCCGCGAGGGGAGGGGGGACAAGAAAAGTGGGATCTTCAAA
ACACCTCAAGGAGAGTTGTC

Downstream 100 bases:

>100_bases
AAATCAAATATTGGGGCGTCGCCAAGCGGTAAGGCAACGGGTTTTGATCCCGTCATTCCCAGGTTCGATCCCTGGCGCCC
CAGCCAAACAATTCAGGGGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKKLQLLAPAKVNYRLDVLGKRPDGYHELRMIMQRVDLCDEIEIALSDSPGIRVTCGRKGVPDGPGNIAWRAADALLKLS
DKEVGIEITIAKKIPVGAGLGGGSSDAATVLMGVNELLELGLTDERLMEIGVKLGADVPFFVFKKPALAEGIGDRLTALE
EVPSLWVVLVNPGIHVSTAWVYQNLRLTTPDPATIIPRSYSSLNEVCELLSNDLEPVTCGRFPLVSELKEVLLTAGARGS
LMSGSGSTVFGLFESESAARHAAAEIAKARGWFAAAVRTI

Sequences:

>Translated_280_residues
MKKLQLLAPAKVNYRLDVLGKRPDGYHELRMIMQRVDLCDEIEIALSDSPGIRVTCGRKGVPDGPGNIAWRAADALLKLS
DKEVGIEITIAKKIPVGAGLGGGSSDAATVLMGVNELLELGLTDERLMEIGVKLGADVPFFVFKKPALAEGIGDRLTALE
EVPSLWVVLVNPGIHVSTAWVYQNLRLTTPDPATIIPRSYSSLNEVCELLSNDLEPVTCGRFPLVSELKEVLLTAGARGS
LMSGSGSTVFGLFESESAARHAAAEIAKARGWFAAAVRTI
>Mature_280_residues
MKKLQLLAPAKVNYRLDVLGKRPDGYHELRMIMQRVDLCDEIEIALSDSPGIRVTCGRKGVPDGPGNIAWRAADALLKLS
DKEVGIEITIAKKIPVGAGLGGGSSDAATVLMGVNELLELGLTDERLMEIGVKLGADVPFFVFKKPALAEGIGDRLTALE
EVPSLWVVLVNPGIHVSTAWVYQNLRLTTPDPATIIPRSYSSLNEVCELLSNDLEPVTCGRFPLVSELKEVLLTAGARGS
LMSGSGSTVFGLFESESAARHAAAEIAKARGWFAAAVRTI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=265, Percent_Identity=38.1132075471698, Blast_Score=137, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_GEOBB (B5EHY0)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139575.1
- ProteinModelPortal:   B5EHY0
- SMR:   B5EHY0
- GeneID:   6782774
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2775
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- ProtClustDB:   PRK14614
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29931; Mature: 29931

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLQLLAPAKVNYRLDVLGKRPDGYHELRMIMQRVDLCDEIEIALSDSPGIRVTCGRKG
CCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCC
VPDGPGNIAWRAADALLKLSDKEVGIEITIAKKIPVGAGLGGGSSDAATVLMGVNELLEL
CCCCCCCEEHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
GLTDERLMEIGVKLGADVPFFVFKKPALAEGIGDRLTALEEVPSLWVVLVNPGIHVSTAW
CCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEE
VYQNLRLTTPDPATIIPRSYSSLNEVCELLSNDLEPVTCGRFPLVSELKEVLLTAGARGS
EEECCEEECCCCCEECCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCCC
LMSGSGSTVFGLFESESAARHAAAEIAKARGWFAAAVRTI
EECCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKKLQLLAPAKVNYRLDVLGKRPDGYHELRMIMQRVDLCDEIEIALSDSPGIRVTCGRKG
CCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCC
VPDGPGNIAWRAADALLKLSDKEVGIEITIAKKIPVGAGLGGGSSDAATVLMGVNELLEL
CCCCCCCEEHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
GLTDERLMEIGVKLGADVPFFVFKKPALAEGIGDRLTALEEVPSLWVVLVNPGIHVSTAW
CCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEE
VYQNLRLTTPDPATIIPRSYSSLNEVCELLSNDLEPVTCGRFPLVSELKEVLLTAGARGS
EEECCEEECCCCCEECCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCCC
LMSGSGSTVFGLFESESAARHAAAEIAKARGWFAAAVRTI
EECCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA