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The map label for this gene is truD [H]

Identifier: 197119149

GI number: 197119149

Start: 3201507

End: 3202769

Strand: Reverse

Name: truD [H]

Synonym: Gbem_2776

Alternate gene names: 197119149

Gene position: 3202769-3201507 (Counterclockwise)

Preceding gene: 197119151

Following gene: 197119148

Centisome position: 69.4

GC content: 63.97

Gene sequence:

>1263_bases
ATGTTGAAAGAGCAGCTGGTACTGGGTATGATGCTGTGGTTGCGGAAACGGATTCAGGGAGGGGCATTGTCCATATATCT
TACAGCAGAGGTCCCGGGGACCGGCGGCACCATCAAGGGGAGCCCGGAAGATTTCCTGGTCGAGGAGATCCCGGCCTATC
TGCCGAGCGGGCAGGGGGAGCACTGTTTCGCGGTCCTGGAAAAGCGCGGCATTGCGACGCTTGAGGCGCTGCGCCGCATC
GCGAAGACCCTGGGTGTCCAGGAGCGCGATTTGGGCTACGCCGGGATGAAGGATGCCATTGGCGTCACCCGTCAAACCGT
CTCCATACCGCGCGTGGCGCCCGATAAGGTGCTGGCGCTTGAGATTCCGGGGATCAAGATCCTCTCGGCCCGTATGCATG
GCAACAAGTTGAGGCTCGGTCACCTCAAGGGAAACCGTTTCGTCATCAGGGTCCGCGACGTGGCCCAAGGCGCGGTCAAG
AATGCGGAGGCCGCGTTGGAGGTCATGACCAGGCGCGGGGTACCCAACCGTTTCGGGGTCCAGCGCTACGGTGCCCAAGG
CAACACCCACGAGATAGGGGCGGCGATGCTGCGCCGCGAGTTCAAGTCGGCGGTGGACCGGCTGATCGGCGACCCGGCTG
CGGTGACCGACGAGCGCTGGCGCCAGGCCATAGAGGCGTACCGGCGCGGCGAAGTGGAGGAAAGCCTGGCGCTTTTCCCG
GGGCATTTCCGGGTCGAGCGCGAACTGCTCACCCGGCTAGTGCAGCGTCCGGACGGCTTCGAGAGGGCCTTCAACTCGGT
GCAGCCGCGCATGAAGAGGTTGTATCTCTCCGCCTTCCAGTCCTCCCTCTTCGACCTGGTGTTGGAAAAAAGGCTCGATA
GCTTCGACAAGGTGAACGTCGGCGACATCGCCTTCAAGCACGAGAACGGCGCCCGTTTCCTGGTGCAGGATCTGGCAGCG
GAGGCGCCGCGCGCCGAAGCCTTCGACATTTCCCCCACCGGCCCCATGTTCGGCTGCACCATGATGGAGCCTCACGGCGC
GCAGGGCGAGCTTGAGCGCGAGGTGCTGGCAGCGCAAGAGTTAACCCTGGAGAGCTTCAACCTCTCGGGAGGTCTGCGTA
TGGAAGGGGAGCGGCGCCCATTGCGGGTCCCTATCGCCGGGGCGGATGTGCAGCAGGAGGGCTCCGACCTGTTGGTCGGC
TTCTCGCTGCCGCGAGGCGCCTACGCCACCTGCGTCATGAGCGAGATAATGAAGAGCGAATAG

Upstream 100 bases:

>100_bases
GCTGTCAGAGAAGCATTATGACGATGCTTATTGGTCGGCATTACGGGCGAATTCTTTAGTCGCCGTTCCTGCAGATTCTG
TCCGCGGGTGGAATGGAAAG

Downstream 100 bases:

>100_bases
GTAGGTGTGCAGGGATTCCCTCACCCCCGCCCTTCTCCCAGAGGGCGAGGGGAGACTGTGCTGCCGAGCTTTTTCCAGCT
CTCCCCCTCCCGCGAGGGGA

Product: tRNA pseudouridine 13 synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MLKEQLVLGMMLWLRKRIQGGALSIYLTAEVPGTGGTIKGSPEDFLVEEIPAYLPSGQGEHCFAVLEKRGIATLEALRRI
AKTLGVQERDLGYAGMKDAIGVTRQTVSIPRVAPDKVLALEIPGIKILSARMHGNKLRLGHLKGNRFVIRVRDVAQGAVK
NAEAALEVMTRRGVPNRFGVQRYGAQGNTHEIGAAMLRREFKSAVDRLIGDPAAVTDERWRQAIEAYRRGEVEESLALFP
GHFRVERELLTRLVQRPDGFERAFNSVQPRMKRLYLSAFQSSLFDLVLEKRLDSFDKVNVGDIAFKHENGARFLVQDLAA
EAPRAEAFDISPTGPMFGCTMMEPHGAQGELEREVLAAQELTLESFNLSGGLRMEGERRPLRVPIAGADVQQEGSDLLVG
FSLPRGAYATCVMSEIMKSE

Sequences:

>Translated_420_residues
MLKEQLVLGMMLWLRKRIQGGALSIYLTAEVPGTGGTIKGSPEDFLVEEIPAYLPSGQGEHCFAVLEKRGIATLEALRRI
AKTLGVQERDLGYAGMKDAIGVTRQTVSIPRVAPDKVLALEIPGIKILSARMHGNKLRLGHLKGNRFVIRVRDVAQGAVK
NAEAALEVMTRRGVPNRFGVQRYGAQGNTHEIGAAMLRREFKSAVDRLIGDPAAVTDERWRQAIEAYRRGEVEESLALFP
GHFRVERELLTRLVQRPDGFERAFNSVQPRMKRLYLSAFQSSLFDLVLEKRLDSFDKVNVGDIAFKHENGARFLVQDLAA
EAPRAEAFDISPTGPMFGCTMMEPHGAQGELEREVLAAQELTLESFNLSGGLRMEGERRPLRVPIAGADVQQEGSDLLVG
FSLPRGAYATCVMSEIMKSE
>Mature_420_residues
MLKEQLVLGMMLWLRKRIQGGALSIYLTAEVPGTGGTIKGSPEDFLVEEIPAYLPSGQGEHCFAVLEKRGIATLEALRRI
AKTLGVQERDLGYAGMKDAIGVTRQTVSIPRVAPDKVLALEIPGIKILSARMHGNKLRLGHLKGNRFVIRVRDVAQGAVK
NAEAALEVMTRRGVPNRFGVQRYGAQGNTHEIGAAMLRREFKSAVDRLIGDPAAVTDERWRQAIEAYRRGEVEESLALFP
GHFRVERELLTRLVQRPDGFERAFNSVQPRMKRLYLSAFQSSLFDLVLEKRLDSFDKVNVGDIAFKHENGARFLVQDLAA
EAPRAEAFDISPTGPMFGCTMMEPHGAQGELEREVLAAQELTLESFNLSGGLRMEGERRPLRVPIAGADVQQEGSDLLVG
FSLPRGAYATCVMSEIMKSE

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI148762985, Length=397, Percent_Identity=25.6926952141058, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI148762961, Length=397, Percent_Identity=25.6926952141058, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI148762948, Length=397, Percent_Identity=25.6926952141058, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI50727002, Length=405, Percent_Identity=24.1975308641975, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1789102, Length=167, Percent_Identity=37.125748502994, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557232, Length=259, Percent_Identity=24.3243243243243, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324817, Length=276, Percent_Identity=30.0724637681159, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355799, Length=254, Percent_Identity=27.5590551181102, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 46423; Mature: 46423

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEQLVLGMMLWLRKRIQGGALSIYLTAEVPGTGGTIKGSPEDFLVEEIPAYLPSGQGE
CCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
HCFAVLEKRGIATLEALRRIAKTLGVQERDLGYAGMKDAIGVTRQTVSIPRVAPDKVLAL
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCEEEE
EIPGIKILSARMHGNKLRLGHLKGNRFVIRVRDVAQGAVKNAEAALEVMTRRGVPNRFGV
ECCCEEEEEEEECCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QRYGAQGNTHEIGAAMLRREFKSAVDRLIGDPAAVTDERWRQAIEAYRRGEVEESLALFP
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCC
GHFRVERELLTRLVQRPDGFERAFNSVQPRMKRLYLSAFQSSLFDLVLEKRLDSFDKVNV
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GDIAFKHENGARFLVQDLAAEAPRAEAFDISPTGPMFGCTMMEPHGAQGELEREVLAAQE
CCEEEECCCCCHHHHHHHHHCCCCCCEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
LTLESFNLSGGLRMEGERRPLRVPIAGADVQQEGSDLLVGFSLPRGAYATCVMSEIMKSE
HHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKEQLVLGMMLWLRKRIQGGALSIYLTAEVPGTGGTIKGSPEDFLVEEIPAYLPSGQGE
CCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
HCFAVLEKRGIATLEALRRIAKTLGVQERDLGYAGMKDAIGVTRQTVSIPRVAPDKVLAL
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCEEEE
EIPGIKILSARMHGNKLRLGHLKGNRFVIRVRDVAQGAVKNAEAALEVMTRRGVPNRFGV
ECCCEEEEEEEECCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QRYGAQGNTHEIGAAMLRREFKSAVDRLIGDPAAVTDERWRQAIEAYRRGEVEESLALFP
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCC
GHFRVERELLTRLVQRPDGFERAFNSVQPRMKRLYLSAFQSSLFDLVLEKRLDSFDKVNV
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GDIAFKHENGARFLVQDLAAEAPRAEAFDISPTGPMFGCTMMEPHGAQGELEREVLAAQE
CCEEEECCCCCHHHHHHHHHCCCCCCEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
LTLESFNLSGGLRMEGERRPLRVPIAGADVQQEGSDLLVGFSLPRGAYATCVMSEIMKSE
HHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA