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The map label for this gene is pleD [H]

Identifier: 197119088

GI number: 197119088

Start: 3134002

End: 3134946

Strand: Reverse

Name: pleD [H]

Synonym: Gbem_2715

Alternate gene names: 197119088

Gene position: 3134946-3134002 (Counterclockwise)

Preceding gene: 197119089

Following gene: 197119086

Centisome position: 67.93

GC content: 63.7

Gene sequence:

>945_bases
ATGGACTTACTGGTTGTTGACGACGAGGAAACCCTGAGAAGCGTGGTGTCGCAGGTTCTTTCCGCCGACGGCTTCACGGT
GGCGGAGGCGGCCAGCGGCGAGGAGGCGCTGGAGGCTTTCAGGAGCGTGAGCCATCCCCTCGTGATCACTGACATAAGGA
TGGGGGGGATGAGCGGGATCGAGCTTCTAACCGAGATCAAGGAGCACAATCCCGACACCCAGGTGGTGATCATGACCAGC
CACGCCTCGCTCGACAGCGCGCTCACCGCGCTCAGAGCCGGCGCCTACGACTACCTGGTGAAGCCGTTCGAGAGCCTGGA
CCTGATCTCCGCGGTGGCGGGGCGGGCGGCGGAGAAGGCTCGCCTGGTGACGCAGAACCGGGAGCTCTTGGAGCAATTGA
CCCGCGCCAACGCCGAGCTAAAGGAGGCGAACCTGGCACTCAAGGAGCTGGCGGTGCATGACGGGCTCACCGCGCTTTAC
AACCATCGCTACTTCGAGGAGGCGTTTGTTTTGGAGGTGGCCAGGTCCAAGCGCTACGGGAAGCAGTGCAGCCTCATCTT
CATGGATGTCGACAACTTCAAGAGGTACAACGACACCAACGGGCATCCGCAGGGGGACGCGCTTTTGAAGACCCTGGCGC
AGCTCATGACGACGCACCTGCGGATCTGCGACACGGCTGCGCGCTACGGCGGAGAGGAGTTCGTGCTGCTGCTCCCGGAG
ACGCCCAAGGAGGCGGCGCTGGGCGTGGCGGAGGAACTCAGGCGCAGGGTCGCGGAGCACCCGTTCCCCGGCGGGGAGAC
CCAGCCCTTGGGGAAGGTGACCATCAGCATCGGCGTAGCCGCCTATCCCAGGGACGGGATGGACGCGCCGGAACTTTTGG
ACTTCGCCGACCAGCTCCTCTACAAGGCGAAGAACAGCGGGAAGAACATGGTGGTATGGAACTGA

Upstream 100 bases:

>100_bases
CGGCGGCGAGATTCACCTGGAGAGCGAGGAGGGGTTGGGGACTACCTTCATCATCACCCTACCACCCTCCGCGGCAGCCA
ATGGCTAAAGGAGGACGCGC

Downstream 100 bases:

>100_bases
GAAACCCGAGAACGGCTAAACCATTTAGCCACGGAGAAAATCTGAGAGAGTCAAAAACCAAAACCGCTCACAAGGAGAAC
TTCTGAGGACTTCTGAGAAA

Product: response receiver-modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MDLLVVDDEETLRSVVSQVLSADGFTVAEAASGEEALEAFRSVSHPLVITDIRMGGMSGIELLTEIKEHNPDTQVVIMTS
HASLDSALTALRAGAYDYLVKPFESLDLISAVAGRAAEKARLVTQNRELLEQLTRANAELKEANLALKELAVHDGLTALY
NHRYFEEAFVLEVARSKRYGKQCSLIFMDVDNFKRYNDTNGHPQGDALLKTLAQLMTTHLRICDTAARYGGEEFVLLLPE
TPKEAALGVAEELRRRVAEHPFPGGETQPLGKVTISIGVAAYPRDGMDAPELLDFADQLLYKAKNSGKNMVVWN

Sequences:

>Translated_314_residues
MDLLVVDDEETLRSVVSQVLSADGFTVAEAASGEEALEAFRSVSHPLVITDIRMGGMSGIELLTEIKEHNPDTQVVIMTS
HASLDSALTALRAGAYDYLVKPFESLDLISAVAGRAAEKARLVTQNRELLEQLTRANAELKEANLALKELAVHDGLTALY
NHRYFEEAFVLEVARSKRYGKQCSLIFMDVDNFKRYNDTNGHPQGDALLKTLAQLMTTHLRICDTAARYGGEEFVLLLPE
TPKEAALGVAEELRRRVAEHPFPGGETQPLGKVTISIGVAAYPRDGMDAPELLDFADQLLYKAKNSGKNMVVWN
>Mature_314_residues
MDLLVVDDEETLRSVVSQVLSADGFTVAEAASGEEALEAFRSVSHPLVITDIRMGGMSGIELLTEIKEHNPDTQVVIMTS
HASLDSALTALRAGAYDYLVKPFESLDLISAVAGRAAEKARLVTQNRELLEQLTRANAELKEANLALKELAVHDGLTALY
NHRYFEEAFVLEVARSKRYGKQCSLIFMDVDNFKRYNDTNGHPQGDALLKTLAQLMTTHLRICDTAARYGGEEFVLLLPE
TPKEAALGVAEELRRRVAEHPFPGGETQPLGKVTISIGVAAYPRDGMDAPELLDFADQLLYKAKNSGKNMVVWN

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=196, Percent_Identity=33.1632653061224, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=37.5757575757576, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=195, Percent_Identity=37.9487179487179, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788381, Length=230, Percent_Identity=32.1739130434783, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=32.1637426900585, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790299, Length=111, Percent_Identity=43.2432432432432, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788550, Length=132, Percent_Identity=36.3636363636364, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=44.2307692307692, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=36.3095238095238, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=31.2101910828025, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=32.9268292682927, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788905, Length=110, Percent_Identity=35.4545454545455, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=194, Percent_Identity=29.8969072164948, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=31.2138728323699, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787487, Length=105, Percent_Identity=34.2857142857143, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=115, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788191, Length=111, Percent_Identity=36.036036036036, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789402, Length=103, Percent_Identity=33.9805825242718, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082012, Length=112, Percent_Identity=31.25, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34517; Mature: 34517

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLVVDDEETLRSVVSQVLSADGFTVAEAASGEEALEAFRSVSHPLVITDIRMGGMSGI
CCEEEECCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHH
ELLTEIKEHNPDTQVVIMTSHASLDSALTALRAGAYDYLVKPFESLDLISAVAGRAAEKA
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHH
RLVTQNRELLEQLTRANAELKEANLALKELAVHDGLTALYNHRYFEEAFVLEVARSKRYG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
KQCSLIFMDVDNFKRYNDTNGHPQGDALLKTLAQLMTTHLRICDTAARYGGEEFVLLLPE
CCEEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
TPKEAALGVAEELRRRVAEHPFPGGETQPLGKVTISIGVAAYPRDGMDAPELLDFADQLL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHH
YKAKNSGKNMVVWN
HHHHCCCCEEEEEC
>Mature Secondary Structure
MDLLVVDDEETLRSVVSQVLSADGFTVAEAASGEEALEAFRSVSHPLVITDIRMGGMSGI
CCEEEECCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHH
ELLTEIKEHNPDTQVVIMTSHASLDSALTALRAGAYDYLVKPFESLDLISAVAGRAAEKA
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHH
RLVTQNRELLEQLTRANAELKEANLALKELAVHDGLTALYNHRYFEEAFVLEVARSKRYG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
KQCSLIFMDVDNFKRYNDTNGHPQGDALLKTLAQLMTTHLRICDTAARYGGEEFVLLLPE
CCEEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
TPKEAALGVAEELRRRVAEHPFPGGETQPLGKVTISIGVAAYPRDGMDAPELLDFADQLL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHH
YKAKNSGKNMVVWN
HHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA