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The map label for this gene is zraS [H]

Identifier: 197119089

GI number: 197119089

Start: 3134959

End: 3136737

Strand: Reverse

Name: zraS [H]

Synonym: Gbem_2716

Alternate gene names: 197119089

Gene position: 3136737-3134959 (Counterclockwise)

Preceding gene: 197119090

Following gene: 197119088

Centisome position: 67.97

GC content: 63.8

Gene sequence:

>1779_bases
ATGCATACCAGATTAAAATTTCCGCTCAGGTTCAAGATGCTCCTCTCCCAGTTGCTGGTGGTGTCGGTGGTGCTGAGCCT
GATCACCTTCACCATGGCGAACCTGTTCCAGGTCGACAAGACCGCATACATCCACGACCTCACTTCGACGGTGGTGCTGC
ATACGGCGGAGGAGGCGAACGCGCTCCTGGCGGGATACCGGGAGCGGTTGAAGCTCTTCGGTCGCGTCTTGGCCGAGCCG
GAGCTTTCGGGGCGGGACCAGGTGCTGCAGGGCTTTTTCGAGGAGTTCCGCGACTTCGTGCTGGTTACCCGCAGCGGCCC
GGGCGGCGAACAGACCGTGTACGACGGCGCTGCGCTGCAGGCGGTCGGGGTGACCAAAGAGGAGATAGTGGCGAACCTGC
AGGCCCATCCGGCGCCGGAATCCATTCCGGCAGGGCAGGTGTACCTGGTGAATTCCACGCTCTCGCCCAAGCTTCCCACG
CTCACCCTCACCATTTCCGAGCCTGCGGCGGGGGGGGCGCCGGTGGTAACGACGGCGGTGCTGCGCCTGGACCGGTTGCA
GGAGCTGGCCAAGCGCTCGCGGGTCTTCGACATCTTCCTGTTGGACTCGGCCGGGCGCTACCTGGCGCACAAGGCGCCCG
ACCGGGTTGGGGTTGCAGCCAATGTCGAATGGTGGAACCGGGTGAAGGCCCCGCGCAGCTCCGGGATGACCATGGAGTAC
AAAAAACTCGGCAAGGAGATGGTGGGAGGATTCTCGCGCTGCTCGCTGGGGGGGCTGGTGGTCGGGGTGGAGATACCCAA
GAGCGCCGCCTACCTCACCTCGCGCGAACTGCTGAGCGACCTTTTGCTCCTGTCGCTGGCGCTTCTGGGTGCGGCGGCCC
TTTTGAGCCAGTTCTGGTCGCGGCATTTCACGAGCCCACTGGAGAAGCTCTCCGAGGCGACCCGGATGGTGGGGCAGGGG
CGCTTCGAGATCGAGGTGAAGGCGGAATCAGGGGACGAGATCGGCGCGCTGGCCCGGTCCTTCAACCAGATGGCCGCCGA
GTTGAAAGTGCGCGAAAAGGCCCTCAAGGACCTCTACGGGCAACTGGTTCATTCGGAGAAGATGGCGGCCTTCGGTTCGC
TTGGCGCAGGGATCGCCCATGAGGTGAAGAACCCGCTGGCGGGGATCCTCGGCATCACCCAGCTCTCGCTCAGGGGGGCG
GGGGCCGGGCACCCGCTGGAGAAGAACCTTTTGATCATCGAGAAAGAGACCAAGCGTTGCAAGACCATCATCGAGCACCT
GCTCAAGTTTGCGCGCCAGGAGCAGGTCGAGTTCAGCGAGGTCGACCTGCAGCAGGTGGTGGCGGATGCTCTTGCCATCG
TCGACCACCAGTTGGGGATCAACAGCATCAAGGTGGAGCAGGAACTGGAGCCGGGGATGCCCACCTGCCGCGGCAACGCG
AACCAGTTGCAGCAGGTGCTCATGAACCTGATGCTCAACGCGCAGCAGGCGATGAGCGGGAAGACCGGGACGGTGAAGCT
TTCCGGGCGCAGGCTGGAAAAGGGGGGAGTGGAATTGCGGGTGGCGGACAACGGCCCCGGTATCAGCAAGGAGATCCAGG
GGAAGATCTTCGATCCCTTCTTCACCACCAAGCCGGCAGGGCAGGGAACGGGGCTCGGCCTCTCGGTCACCTACGGCATC
GTCAAGGATCACGGCGGCGAGATTCACCTGGAGAGCGAGGAGGGGTTGGGGACTACCTTCATCATCACCCTACCACCCTC
CGCGGCAGCCAATGGCTAA

Upstream 100 bases:

>100_bases
TGATCAGGGTGGAGGCCGTCGACCAGGCAGGAAACGCCAGTTACGCCTCCCGGGTCATCTACGGGACCGGCGCCGGACAG
CAAGGCAGATAGAGGACCGG

Downstream 100 bases:

>100_bases
AGGAGGACGCGCATGGACTTACTGGTTGTTGACGACGAGGAAACCCTGAGAAGCGTGGTGTCGCAGGTTCTTTCCGCCGA
CGGCTTCACGGTGGCGGAGG

Product: HAMP domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MHTRLKFPLRFKMLLSQLLVVSVVLSLITFTMANLFQVDKTAYIHDLTSTVVLHTAEEANALLAGYRERLKLFGRVLAEP
ELSGRDQVLQGFFEEFRDFVLVTRSGPGGEQTVYDGAALQAVGVTKEEIVANLQAHPAPESIPAGQVYLVNSTLSPKLPT
LTLTISEPAAGGAPVVTTAVLRLDRLQELAKRSRVFDIFLLDSAGRYLAHKAPDRVGVAANVEWWNRVKAPRSSGMTMEY
KKLGKEMVGGFSRCSLGGLVVGVEIPKSAAYLTSRELLSDLLLLSLALLGAAALLSQFWSRHFTSPLEKLSEATRMVGQG
RFEIEVKAESGDEIGALARSFNQMAAELKVREKALKDLYGQLVHSEKMAAFGSLGAGIAHEVKNPLAGILGITQLSLRGA
GAGHPLEKNLLIIEKETKRCKTIIEHLLKFARQEQVEFSEVDLQQVVADALAIVDHQLGINSIKVEQELEPGMPTCRGNA
NQLQQVLMNLMLNAQQAMSGKTGTVKLSGRRLEKGGVELRVADNGPGISKEIQGKIFDPFFTTKPAGQGTGLGLSVTYGI
VKDHGGEIHLESEEGLGTTFIITLPPSAAANG

Sequences:

>Translated_592_residues
MHTRLKFPLRFKMLLSQLLVVSVVLSLITFTMANLFQVDKTAYIHDLTSTVVLHTAEEANALLAGYRERLKLFGRVLAEP
ELSGRDQVLQGFFEEFRDFVLVTRSGPGGEQTVYDGAALQAVGVTKEEIVANLQAHPAPESIPAGQVYLVNSTLSPKLPT
LTLTISEPAAGGAPVVTTAVLRLDRLQELAKRSRVFDIFLLDSAGRYLAHKAPDRVGVAANVEWWNRVKAPRSSGMTMEY
KKLGKEMVGGFSRCSLGGLVVGVEIPKSAAYLTSRELLSDLLLLSLALLGAAALLSQFWSRHFTSPLEKLSEATRMVGQG
RFEIEVKAESGDEIGALARSFNQMAAELKVREKALKDLYGQLVHSEKMAAFGSLGAGIAHEVKNPLAGILGITQLSLRGA
GAGHPLEKNLLIIEKETKRCKTIIEHLLKFARQEQVEFSEVDLQQVVADALAIVDHQLGINSIKVEQELEPGMPTCRGNA
NQLQQVLMNLMLNAQQAMSGKTGTVKLSGRRLEKGGVELRVADNGPGISKEIQGKIFDPFFTTKPAGQGTGLGLSVTYGI
VKDHGGEIHLESEEGLGTTFIITLPPSAAANG
>Mature_592_residues
MHTRLKFPLRFKMLLSQLLVVSVVLSLITFTMANLFQVDKTAYIHDLTSTVVLHTAEEANALLAGYRERLKLFGRVLAEP
ELSGRDQVLQGFFEEFRDFVLVTRSGPGGEQTVYDGAALQAVGVTKEEIVANLQAHPAPESIPAGQVYLVNSTLSPKLPT
LTLTISEPAAGGAPVVTTAVLRLDRLQELAKRSRVFDIFLLDSAGRYLAHKAPDRVGVAANVEWWNRVKAPRSSGMTMEY
KKLGKEMVGGFSRCSLGGLVVGVEIPKSAAYLTSRELLSDLLLLSLALLGAAALLSQFWSRHFTSPLEKLSEATRMVGQG
RFEIEVKAESGDEIGALARSFNQMAAELKVREKALKDLYGQLVHSEKMAAFGSLGAGIAHEVKNPLAGILGITQLSLRGA
GAGHPLEKNLLIIEKETKRCKTIIEHLLKFARQEQVEFSEVDLQQVVADALAIVDHQLGINSIKVEQELEPGMPTCRGNA
NQLQQVLMNLMLNAQQAMSGKTGTVKLSGRRLEKGGVELRVADNGPGISKEIQGKIFDPFFTTKPAGQGTGLGLSVTYGI
VKDHGGEIHLESEEGLGTTFIITLPPSAAANG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=35.3711790393013, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1788549, Length=262, Percent_Identity=32.824427480916, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1790300, Length=335, Percent_Identity=28.955223880597, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790346, Length=291, Percent_Identity=30.5841924398625, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789808, Length=315, Percent_Identity=26.984126984127, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787894, Length=321, Percent_Identity=26.791277258567, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788393, Length=293, Percent_Identity=24.9146757679181, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=29.9107142857143, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=336, Percent_Identity=26.7857142857143, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789149, Length=333, Percent_Identity=23.1231231231231, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI48994928, Length=227, Percent_Identity=28.6343612334802, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI87082128, Length=219, Percent_Identity=28.7671232876712, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=294, Percent_Identity=25.1700680272109, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=26.1061946902655, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64018; Mature: 64018

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTRLKFPLRFKMLLSQLLVVSVVLSLITFTMANLFQVDKTAYIHDLTSTVVLHTAEEAN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEHHHHH
ALLAGYRERLKLFGRVLAEPELSGRDQVLQGFFEEFRDFVLVTRSGPGGEQTVYDGAALQ
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCEEECCCHHE
AVGVTKEEIVANLQAHPAPESIPAGQVYLVNSTLSPKLPTLTLTISEPAAGGAPVVTTAV
EECCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHH
LRLDRLQELAKRSRVFDIFLLDSAGRYLAHKAPDRVGVAANVEWWNRVKAPRSSGMTMEY
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHCCCCCCCEEECCHHHHHHCCCCCCCCCHHH
KKLGKEMVGGFSRCSLGGLVVGVEIPKSAAYLTSRELLSDLLLLSLALLGAAALLSQFWS
HHHHHHHHCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHFTSPLEKLSEATRMVGQGRFEIEVKAESGDEIGALARSFNQMAAELKVREKALKDLYG
HHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVHSEKMAAFGSLGAGIAHEVKNPLAGILGITQLSLRGAGAGHPLEKNLLIIEKETKRC
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECHHHHH
KTIIEHLLKFARQEQVEFSEVDLQQVVADALAIVDHQLGINSIKVEQELEPGMPTCRGNA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCCCH
NQLQQVLMNLMLNAQQAMSGKTGTVKLSGRRLEKGGVELRVADNGPGISKEIQGKIFDPF
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCCEEEEECCCCCCCCHHHCCCEECCC
FTTKPAGQGTGLGLSVTYGIVKDHGGEIHLESEEGLGTTFIITLPPSAAANG
CCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MHTRLKFPLRFKMLLSQLLVVSVVLSLITFTMANLFQVDKTAYIHDLTSTVVLHTAEEAN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEHHHHH
ALLAGYRERLKLFGRVLAEPELSGRDQVLQGFFEEFRDFVLVTRSGPGGEQTVYDGAALQ
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCEEECCCHHE
AVGVTKEEIVANLQAHPAPESIPAGQVYLVNSTLSPKLPTLTLTISEPAAGGAPVVTTAV
EECCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHH
LRLDRLQELAKRSRVFDIFLLDSAGRYLAHKAPDRVGVAANVEWWNRVKAPRSSGMTMEY
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHCCCCCCCEEECCHHHHHHCCCCCCCCCHHH
KKLGKEMVGGFSRCSLGGLVVGVEIPKSAAYLTSRELLSDLLLLSLALLGAAALLSQFWS
HHHHHHHHCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHFTSPLEKLSEATRMVGQGRFEIEVKAESGDEIGALARSFNQMAAELKVREKALKDLYG
HHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVHSEKMAAFGSLGAGIAHEVKNPLAGILGITQLSLRGAGAGHPLEKNLLIIEKETKRC
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECHHHHH
KTIIEHLLKFARQEQVEFSEVDLQQVVADALAIVDHQLGINSIKVEQELEPGMPTCRGNA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCCCH
NQLQQVLMNLMLNAQQAMSGKTGTVKLSGRRLEKGGVELRVADNGPGISKEIQGKIFDPF
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCCEEEEECCCCCCCCHHHCCCEECCC
FTTKPAGQGTGLGLSVTYGIVKDHGGEIHLESEEGLGTTFIITLPPSAAANG
CCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]