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The map label for this gene is pdxJ

Identifier: 197119011

GI number: 197119011

Start: 3049764

End: 3050483

Strand: Reverse

Name: pdxJ

Synonym: Gbem_2634

Alternate gene names: 197119011

Gene position: 3050483-3049764 (Counterclockwise)

Preceding gene: 197119012

Following gene: 197119010

Centisome position: 66.1

GC content: 63.33

Gene sequence:

>720_bases
GTGGCTAGACTGGGAGTGAACATAGATCACGTGGCGACGCTTCGGCAGGCGAGGGGGGGGACGGAACCGGACCCGGTCGC
AGCCGCCGCCATCGCCGAATTCGCCGGTGCCGACGGTATCACCATCCATCTGCGTGAGGACCGCAGGCACATCCAGGATC
GCGACCTGAAGATCCTGCGCCAGACCGTGCAGACCAGGCTGAACCTGGAGATGGCCGCTACCGACGAGATGGTAGCCATA
GCGCTCGCGGTGAAGCCCGAGGCGTGCACCCTGGTCCCGGAGAAACGGGCCGAGCTGACCACCGAGGGGGGGCTCGACGT
GCGCATCCACCAGGAGGCGCTCAAGGTCGCCATAGAGAAACTGCAGGCAGGGGGGATCATCGTCAGCCTCTTCATCGATC
CGGACCCCGACCAGATCAAGGTGGCCAACAAGATCGGCGCCGACTATATAGAGATCCACACCGGCTCCTTCGCCGAGGCG
AAGAGCTGGAAGGAAGAGGAACTGGAACTGATCAAGATAGAGAACGCGGTAAAGCTCGCCCGCAAGCTCGATCTCGGCGT
CAACGCCGGGCACGGGCTTAGCTACAGCAACGTGAAGAAGGTGGCGGCCATCGGCGGCATCGAGGAATTCAACATCGGGC
ATTCCATCATGTCCCGCGCCATCCTGGTGGGACTGGACCGCGCCGTGCGCGACATGTCCGAGCTGGTGAGATACGCCTGA

Upstream 100 bases:

>100_bases
CTTTTGCGCATCATGCTGGAAGGAAGCGACGAGGGGGATATTCGCTGCTGGGCCAACGACATAGCTTCCATCGTGGCACA
GAAGCTGGGAGGTGAGGCGC

Downstream 100 bases:

>100_bases
GCTTCAGGCGGGAAGAAAGCATCGAACAAAGGAGAAGCAGATGACAAAAGCTAGTGCGCGTCACATTTTAGTAGCTACCG
AACAGGAGTGCGTAGCGCTC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MARLGVNIDHVATLRQARGGTEPDPVAAAAIAEFAGADGITIHLREDRRHIQDRDLKILRQTVQTRLNLEMAATDEMVAI
ALAVKPEACTLVPEKRAELTTEGGLDVRIHQEALKVAIEKLQAGGIIVSLFIDPDPDQIKVANKIGADYIEIHTGSFAEA
KSWKEEELELIKIENAVKLARKLDLGVNAGHGLSYSNVKKVAAIGGIEEFNIGHSIMSRAILVGLDRAVRDMSELVRYA

Sequences:

>Translated_239_residues
MARLGVNIDHVATLRQARGGTEPDPVAAAAIAEFAGADGITIHLREDRRHIQDRDLKILRQTVQTRLNLEMAATDEMVAI
ALAVKPEACTLVPEKRAELTTEGGLDVRIHQEALKVAIEKLQAGGIIVSLFIDPDPDQIKVANKIGADYIEIHTGSFAEA
KSWKEEELELIKIENAVKLARKLDLGVNAGHGLSYSNVKKVAAIGGIEEFNIGHSIMSRAILVGLDRAVRDMSELVRYA
>Mature_238_residues
ARLGVNIDHVATLRQARGGTEPDPVAAAAIAEFAGADGITIHLREDRRHIQDRDLKILRQTVQTRLNLEMAATDEMVAIA
LAVKPEACTLVPEKRAELTTEGGLDVRIHQEALKVAIEKLQAGGIIVSLFIDPDPDQIKVANKIGADYIEIHTGSFAEAK
SWKEEELELIKIENAVKLARKLDLGVNAGHGLSYSNVKKVAAIGGIEEFNIGHSIMSRAILVGLDRAVRDMSELVRYA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=233, Percent_Identity=60.0858369098712, Blast_Score=280, Evalue=7e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_GEOBB (B5EHA6)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139438.1
- ProteinModelPortal:   B5EHA6
- SMR:   B5EHA6
- GeneID:   6782631
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2634
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26010; Mature: 25879

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 70-70 ACT_SITE 191-191 BINDING 7-7 BINDING 18-18 BINDING 45-45 BINDING 50-50 BINDING 100-100 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLGVNIDHVATLRQARGGTEPDPVAAAAIAEFAGADGITIHLREDRRHIQDRDLKILR
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHH
QTVQTRLNLEMAATDEMVAIALAVKPEACTLVPEKRAELTTEGGLDVRIHQEALKVAIEK
HHHHHHHCEEEECCCCEEEEEEEECCCCEEECCCHHHCEECCCCCEEEEHHHHHHHHHHH
LQAGGIIVSLFIDPDPDQIKVANKIGADYIEIHTGSFAEAKSWKEEELELIKIENAVKLA
HHCCCEEEEEEECCCCCCEEEHHHCCCCEEEEECCCCHHHHCCCHHHHHEEEHHHHHHHH
RKLDLGVNAGHGLSYSNVKKVAAIGGIEEFNIGHSIMSRAILVGLDRAVRDMSELVRYA
HHHCCCCCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARLGVNIDHVATLRQARGGTEPDPVAAAAIAEFAGADGITIHLREDRRHIQDRDLKILR
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHH
QTVQTRLNLEMAATDEMVAIALAVKPEACTLVPEKRAELTTEGGLDVRIHQEALKVAIEK
HHHHHHHCEEEECCCCEEEEEEEECCCCEEECCCHHHCEECCCCCEEEEHHHHHHHHHHH
LQAGGIIVSLFIDPDPDQIKVANKIGADYIEIHTGSFAEAKSWKEEELELIKIENAVKLA
HHCCCEEEEEEECCCCCCEEEHHHCCCCEEEEECCCCHHHHCCCHHHHHEEEHHHHHHHH
RKLDLGVNAGHGLSYSNVKKVAAIGGIEEFNIGHSIMSRAILVGLDRAVRDMSELVRYA
HHHCCCCCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA