Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 197119008

GI number: 197119008

Start: 3047424

End: 3048971

Strand: Direct

Name: emrB [H]

Synonym: Gbem_2631

Alternate gene names: 197119008

Gene position: 3047424-3048971 (Clockwise)

Preceding gene: 197119007

Following gene: 197119023

Centisome position: 66.03

GC content: 59.82

Gene sequence:

>1548_bases
ATGAAAAGCGCTGAAGAAAAGATTGTAAACAAGTGGCTCATCACCATCACGGTGATGCTGCCGGCGATCATGGAGATCGT
CGATACCTCGGTCGCGAACGTGGCGCTCCCGCACATGCAGGGGAGCCTCAACGCCGGGACCGATGAAATCACCTGGGTCC
TCACCTCCTACCTGGTCAGTAACGCAGTCGTCCTCCCCATGACCGGGTGGCTCGCCCGCATGTTCGGGCGCAAAAGGTTC
CTCATCACCTGCATCACCCTCTTCACCCTCGCCTCGCTTCTTTGCGGCGCCGCCCCTTCTCTGGGCATGCTGATCTTCTT
CCGCGTCTTGCAAGGGGCCGCCGGCGGCGCCCTCATCCCCATGAGCCAGGCGATCATGATGGAAACCTTCCCCCCCTATC
AACAGGGAATGGCGATGGCCATCTTCGGCGTAGGCGCCATGTTCGGCCCCATCATCGGCCCGGCGTTGGGCGGCTGGATC
ACCGACAACATGAGCTGGCGCTGGATCTTCTACATCAACATCCCCATCGGGGTCATCGCGGTCATCATGGCTTCCTTCTT
CATCTACGACCCGAGCTACCTGAAACGTACCAAGGTCGCCATCGACTACTGGGGGCTCGCCCTTCTCACCGTGGGGCTGG
GAGCCCTGCAGATAGTGCTCGACAAAGGGCAGCAGGACGACTGGTTCAACTCCCCTTTCATCGTCGGCTGCGCAGTGGTC
ACAGCAATCGCGCTTTCCGCCCTTGTTTACGTCGAATTGACCCACCCGCATCCCATCGTGAACCTGAGGCTCTTCAAGAA
CGTCTCCTTCTCGTCGGGGAACCTGATCATGTTCGCCGTGGGATTCTGCCTTTACAGCTCCATCATGCTGATCCCGCTGT
TCCTGCAGACCCTCATGGGTTACAACGCCACCATGGCCGGCATGGTCCTCGCTCCGGGCGGCGTCGCCACCCTCATCTGC
ATGCCATTCGTGGGAGCGGCGATCCAGCGCTACGACGGCAGGAAGGTCGTTTTCATGGGGCTTATCATCGGGGCTACTTC
CATGTTCATCATGCAGCGTTTCACCCTGCAGGCGGCCTACATGGACTTCGTCTGGCCTCGGGTGGTGCTGGGCGTGGGCC
TTGCCATGATCTTCGTGCCCCTGACCACGGTCACCCTGGCAACCATTTCCAAAGAAGAGATGGGGAACGCGACCGGCATC
TTCAACCTGCTCAGAAACATCGGCGGGAGCGTCGGCATAGCAATCGCGGCCACCATGCTGGCGCGTTACTCGCAGTTTTA
TCAGACCAACCTGGTCGCACACGTAAACCCGTACAACCCGCTGTTCCAATCCCAGCTCGCGACGCTGAAGGGGGCGCTCA
TGGGGCGCGGCCTCGATCCTGTCGCTGCCGAGAAAGGGGCCATGACAGTACTTTACGGGACCGTAAGCCGGCAGGCCTAC
ATGCTCTCCTACAACAGGATCTTCTTCATCGTCGGCTTGGCGTTCCTCATCATCATTCCGCTTTTGTTTCTGTTGAAAAA
GCCCGCCAAGCACCTGCCGCCGGCGTAA

Upstream 100 bases:

>100_bases
CCCCTCCCGTCAAGGGAGGGGAGACCACAAAGCACCGGCTACACCGGTGATACATTTTCCCCCTGATGCTGCGAGTTGAA
GGACACCCCCTGGCGGCCCC

Downstream 100 bases:

>100_bases
GGCAATTGACAATTGACAATTGACAATTGACAACGTTTCCTCAGTCCACCGAGTCCATTTCGTCCATAGGGTCCATGCGG
TCCATTGCAGCAGACAAGGC

Product: RND family multidrug resistance efflux pump inner membrane protein EmrB

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MKSAEEKIVNKWLITITVMLPAIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWLARMFGRKRF
LITCITLFTLASLLCGAAPSLGMLIFFRVLQGAAGGALIPMSQAIMMETFPPYQQGMAMAIFGVGAMFGPIIGPALGGWI
TDNMSWRWIFYINIPIGVIAVIMASFFIYDPSYLKRTKVAIDYWGLALLTVGLGALQIVLDKGQQDDWFNSPFIVGCAVV
TAIALSALVYVELTHPHPIVNLRLFKNVSFSSGNLIMFAVGFCLYSSIMLIPLFLQTLMGYNATMAGMVLAPGGVATLIC
MPFVGAAIQRYDGRKVVFMGLIIGATSMFIMQRFTLQAAYMDFVWPRVVLGVGLAMIFVPLTTVTLATISKEEMGNATGI
FNLLRNIGGSVGIAIAATMLARYSQFYQTNLVAHVNPYNPLFQSQLATLKGALMGRGLDPVAAEKGAMTVLYGTVSRQAY
MLSYNRIFFIVGLAFLIIIPLLFLLKKPAKHLPPA

Sequences:

>Translated_515_residues
MKSAEEKIVNKWLITITVMLPAIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWLARMFGRKRF
LITCITLFTLASLLCGAAPSLGMLIFFRVLQGAAGGALIPMSQAIMMETFPPYQQGMAMAIFGVGAMFGPIIGPALGGWI
TDNMSWRWIFYINIPIGVIAVIMASFFIYDPSYLKRTKVAIDYWGLALLTVGLGALQIVLDKGQQDDWFNSPFIVGCAVV
TAIALSALVYVELTHPHPIVNLRLFKNVSFSSGNLIMFAVGFCLYSSIMLIPLFLQTLMGYNATMAGMVLAPGGVATLIC
MPFVGAAIQRYDGRKVVFMGLIIGATSMFIMQRFTLQAAYMDFVWPRVVLGVGLAMIFVPLTTVTLATISKEEMGNATGI
FNLLRNIGGSVGIAIAATMLARYSQFYQTNLVAHVNPYNPLFQSQLATLKGALMGRGLDPVAAEKGAMTVLYGTVSRQAY
MLSYNRIFFIVGLAFLIIIPLLFLLKKPAKHLPPA
>Mature_515_residues
MKSAEEKIVNKWLITITVMLPAIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWLARMFGRKRF
LITCITLFTLASLLCGAAPSLGMLIFFRVLQGAAGGALIPMSQAIMMETFPPYQQGMAMAIFGVGAMFGPIIGPALGGWI
TDNMSWRWIFYINIPIGVIAVIMASFFIYDPSYLKRTKVAIDYWGLALLTVGLGALQIVLDKGQQDDWFNSPFIVGCAVV
TAIALSALVYVELTHPHPIVNLRLFKNVSFSSGNLIMFAVGFCLYSSIMLIPLFLQTLMGYNATMAGMVLAPGGVATLIC
MPFVGAAIQRYDGRKVVFMGLIIGATSMFIMQRFTLQAAYMDFVWPRVVLGVGLAMIFVPLTTVTLATISKEEMGNATGI
FNLLRNIGGSVGIAIAATMLARYSQFYQTNLVAHVNPYNPLFQSQLATLKGALMGRGLDPVAAEKGAMTVLYGTVSRQAY
MLSYNRIFFIVGLAFLIIIPLLFLLKKPAKHLPPA

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=499, Percent_Identity=36.2725450901804, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=34.8697394789579, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1790195, Length=420, Percent_Identity=28.3333333333333, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=27.0531400966184, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788392, Length=403, Percent_Identity=29.7766749379653, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319770, Length=391, Percent_Identity=27.8772378516624, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325455, Length=138, Percent_Identity=31.1594202898551, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=442, Percent_Identity=23.7556561085973, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=33.8345864661654, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=447, Percent_Identity=21.4765100671141, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56008; Mature: 56008

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSAEEKIVNKWLITITVMLPAIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
NAVVLPMTGWLARMFGRKRFLITCITLFTLASLLCGAAPSLGMLIFFRVLQGAAGGALIP
CCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
MSQAIMMETFPPYQQGMAMAIFGVGAMFGPIIGPALGGWITDNMSWRWIFYINIPIGVIA
HHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEEEECCHHHHH
VIMASFFIYDPSYLKRTKVAIDYWGLALLTVGLGALQIVLDKGQQDDWFNSPFIVGCAVV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
TAIALSALVYVELTHPHPIVNLRLFKNVSFSSGNLIMFAVGFCLYSSIMLIPLFLQTLMG
HHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
YNATMAGMVLAPGGVATLICMPFVGAAIQRYDGRKVVFMGLIIGATSMFIMQRFTLQAAY
CCCHHHCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
MDFVWPRVVLGVGLAMIFVPLTTVTLATISKEEMGNATGIFNLLRNIGGSVGIAIAATML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
ARYSQFYQTNLVAHVNPYNPLFQSQLATLKGALMGRGLDPVAAEKGAMTVLYGTVSRQAY
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCCCCEE
MLSYNRIFFIVGLAFLIIIPLLFLLKKPAKHLPPA
EEHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCC
>Mature Secondary Structure
MKSAEEKIVNKWLITITVMLPAIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
NAVVLPMTGWLARMFGRKRFLITCITLFTLASLLCGAAPSLGMLIFFRVLQGAAGGALIP
CCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
MSQAIMMETFPPYQQGMAMAIFGVGAMFGPIIGPALGGWITDNMSWRWIFYINIPIGVIA
HHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEEEECCHHHHH
VIMASFFIYDPSYLKRTKVAIDYWGLALLTVGLGALQIVLDKGQQDDWFNSPFIVGCAVV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
TAIALSALVYVELTHPHPIVNLRLFKNVSFSSGNLIMFAVGFCLYSSIMLIPLFLQTLMG
HHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
YNATMAGMVLAPGGVATLICMPFVGAAIQRYDGRKVVFMGLIIGATSMFIMQRFTLQAAY
CCCHHHCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
MDFVWPRVVLGVGLAMIFVPLTTVTLATISKEEMGNATGIFNLLRNIGGSVGIAIAATML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
ARYSQFYQTNLVAHVNPYNPLFQSQLATLKGALMGRGLDPVAAEKGAMTVLYGTVSRQAY
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCCCCEE
MLSYNRIFFIVGLAFLIIIPLLFLLKKPAKHLPPA
EEHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]