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The map label for this gene is emrA [H]

Identifier: 197119007

GI number: 197119007

Start: 3046157

End: 3047257

Strand: Direct

Name: emrA [H]

Synonym: Gbem_2630

Alternate gene names: 197119007

Gene position: 3046157-3047257 (Clockwise)

Preceding gene: 197119006

Following gene: 197119008

Centisome position: 66.0

GC content: 64.85

Gene sequence:

>1101_bases
ATGGCTGAAGAAACAAACCAGACTACTGAAAATAACGAAACGAATCCGGCGCCGGGCGGCGAGCGGAGGGGGCTCAACAA
GAGACAGCGCGGCGGCATCGTGCTCCTCGTGATCATCGTTATCGGCGCGCTTTTAGGCGTGCACCGCTGGGTCCGCAGCA
AGACCCACATCGAGACGGACAACGCCTTCATCGAGTCGCATGTCCACTCCATCGCGAGCCGGGTTCCCGGACTGGTGCAC
CGGGTTGCGGTGGTGGACAACCAGTTCGTGCGCAAGGGGGACCTGCTGGTCGAACTCGACCCCTCGGACTACCGGGCGCG
CGAGAACAGCGCCGCGGCTTCCGTGGAAATGGCCAAGAACGAGACCTCAGGCGACTACGCGCAAGTGGAGAGCGCCCGGG
CCAACATCGGCTTGGCGGCCGCCCGCCTGGAGCAGGCTAACCTGGACCTGCGACGGGCCGAGGCCCTTTATGCCAAGGAA
GTTATCCCCCGCGAGCAGTTGGAGCGTCAGCGCACCGCGCACCGTGTCGCGCAGATGCAGCTCAAGGAGGCGCAGGAAGC
GGAAAGAAAGGCAAAGGCGATGATCGGCATTTCCGGCACCGGCTCCAAGGACGCCAGGGTTGCCGAGAAGCAGGCGGAGT
TGGAGACCGCGCACCTGAACCTCTCCTATACCCGCATCGTCGCCCCCGGCGACGGCTTCGTGACCAGGAAGGGTGTCGAG
CCGGGAAACTACGTGCAGCCGGGCCAGGCGCTGATGGCCGTGGTGCCGCTGGAGGGGGCCTGGATCACCGCGAACTACAA
GGAAAGCCAACTGGCCAAGGTGCGGCCGGGGCAGAACGTCGAGTTCACCGTGGACACCTACCCGGGGCGAACCTTCACCG
GAAAGGTGGAAAGCATCATGGCTGGAACCGGCGCGGCCTTCTCGCTGCTGCCGCCTGAAAACGCCACGGGGAACTACGTC
AAGGTGACCCAGAGGATTCCGGTCAGGATCGCCATCGACCGCAGAAGCGACCCCGAGCACCTGTTGCGGGTCGGGATGAG
CGTGGTCCCCACCATCCTCACCGGCCAGACCTTCGGCGACGTGGTCGGTTTCGGCCGCTAA

Upstream 100 bases:

>100_bases
TGCAGGTAGCCGCAGCCCGGGTGAAACGGGCGACGGGAGAGCTGTAACGCAAAAGGCAGGACTTATCTGACGCAACCAAA
ATTTCTTGAGGTTCATATAA

Downstream 100 bases:

>100_bases
ACAAACCGTTACTCAGCTTTTCCTGATAGCGGCAGGCTCCTTTATGGCAGCTTCACCCACCCCCAGCCCCTCCCGTCAAG
GGAGGGGAGACCACAAAGCA

Product: RND family multidrug resistance efflux pump membrane fusion protein EmrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAEETNQTTENNETNPAPGGERRGLNKRQRGGIVLLVIIVIGALLGVHRWVRSKTHIETDNAFIESHVHSIASRVPGLVH
RVAVVDNQFVRKGDLLVELDPSDYRARENSAAASVEMAKNETSGDYAQVESARANIGLAAARLEQANLDLRRAEALYAKE
VIPREQLERQRTAHRVAQMQLKEAQEAERKAKAMIGISGTGSKDARVAEKQAELETAHLNLSYTRIVAPGDGFVTRKGVE
PGNYVQPGQALMAVVPLEGAWITANYKESQLAKVRPGQNVEFTVDTYPGRTFTGKVESIMAGTGAAFSLLPPENATGNYV
KVTQRIPVRIAIDRRSDPEHLLRVGMSVVPTILTGQTFGDVVGFGR

Sequences:

>Translated_366_residues
MAEETNQTTENNETNPAPGGERRGLNKRQRGGIVLLVIIVIGALLGVHRWVRSKTHIETDNAFIESHVHSIASRVPGLVH
RVAVVDNQFVRKGDLLVELDPSDYRARENSAAASVEMAKNETSGDYAQVESARANIGLAAARLEQANLDLRRAEALYAKE
VIPREQLERQRTAHRVAQMQLKEAQEAERKAKAMIGISGTGSKDARVAEKQAELETAHLNLSYTRIVAPGDGFVTRKGVE
PGNYVQPGQALMAVVPLEGAWITANYKESQLAKVRPGQNVEFTVDTYPGRTFTGKVESIMAGTGAAFSLLPPENATGNYV
KVTQRIPVRIAIDRRSDPEHLLRVGMSVVPTILTGQTFGDVVGFGR
>Mature_365_residues
AEETNQTTENNETNPAPGGERRGLNKRQRGGIVLLVIIVIGALLGVHRWVRSKTHIETDNAFIESHVHSIASRVPGLVHR
VAVVDNQFVRKGDLLVELDPSDYRARENSAAASVEMAKNETSGDYAQVESARANIGLAAARLEQANLDLRRAEALYAKEV
IPREQLERQRTAHRVAQMQLKEAQEAERKAKAMIGISGTGSKDARVAEKQAELETAHLNLSYTRIVAPGDGFVTRKGVEP
GNYVQPGQALMAVVPLEGAWITANYKESQLAKVRPGQNVEFTVDTYPGRTFTGKVESIMAGTGAAFSLLPPENATGNYVK
VTQRIPVRIAIDRRSDPEHLLRVGMSVVPTILTGQTFGDVVGFGR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=328, Percent_Identity=36.890243902439, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1788711, Length=340, Percent_Identity=34.1176470588235, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1790519, Length=316, Percent_Identity=30.379746835443, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790024, Length=236, Percent_Identity=27.5423728813559, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790012, Length=233, Percent_Identity=24.8927038626609, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789637, Length=309, Percent_Identity=27.5080906148867, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87081951, Length=304, Percent_Identity=26.6447368421053, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787013, Length=306, Percent_Identity=25.4901960784314, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081796, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39916; Mature: 39785

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEETNQTTENNETNPAPGGERRGLNKRQRGGIVLLVIIVIGALLGVHRWVRSKTHIETD
CCCCCCCCCCCCCCCCCCCCHHCCCCCHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCC
NAFIESHVHSIASRVPGLVHRVAVVDNQFVRKGDLLVELDPSDYRARENSAAASVEMAKN
HHHHHHHHHHHHHHCHHHHHHHHHHCCHHHCCCCEEEEECCCHHCCCCCCHHHHEEECCC
ETSGDYAQVESARANIGLAAARLEQANLDLRRAEALYAKEVIPREQLERQRTAHRVAQMQ
CCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LKEAQEAERKAKAMIGISGTGSKDARVAEKQAELETAHLNLSYTRIVAPGDGFVTRKGVE
HHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHEEEEEEEEEEEEEECCCCCEEECCCC
PGNYVQPGQALMAVVPLEGAWITANYKESQLAKVRPGQNVEFTVDTYPGRTFTGKVESIM
CCCCCCCCCEEEEEEECCCEEEEECCCCCHHHCCCCCCCEEEEEECCCCCEECCHHHHHH
AGTGAAFSLLPPENATGNYVKVTQRIPVRIAIDRRSDPEHLLRVGMSVVPTILTGQTFGD
HCCCCEEEECCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHH
VVGFGR
HHCCCC
>Mature Secondary Structure 
AEETNQTTENNETNPAPGGERRGLNKRQRGGIVLLVIIVIGALLGVHRWVRSKTHIETD
CCCCCCCCCCCCCCCCCCCHHCCCCCHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCC
NAFIESHVHSIASRVPGLVHRVAVVDNQFVRKGDLLVELDPSDYRARENSAAASVEMAKN
HHHHHHHHHHHHHHCHHHHHHHHHHCCHHHCCCCEEEEECCCHHCCCCCCHHHHEEECCC
ETSGDYAQVESARANIGLAAARLEQANLDLRRAEALYAKEVIPREQLERQRTAHRVAQMQ
CCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LKEAQEAERKAKAMIGISGTGSKDARVAEKQAELETAHLNLSYTRIVAPGDGFVTRKGVE
HHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHEEEEEEEEEEEEEECCCCCEEECCCC
PGNYVQPGQALMAVVPLEGAWITANYKESQLAKVRPGQNVEFTVDTYPGRTFTGKVESIM
CCCCCCCCCEEEEEEECCCEEEEECCCCCHHHCCCCCCCEEEEEECCCCCEECCHHHHHH
AGTGAAFSLLPPENATGNYVKVTQRIPVRIAIDRRSDPEHLLRVGMSVVPTILTGQTFGD
HCCCCEEEECCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHH
VVGFGR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]