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The map label for this gene is pilB [H]

Identifier: 197118972

GI number: 197118972

Start: 3005418

End: 3007124

Strand: Reverse

Name: pilB [H]

Synonym: Gbem_2595

Alternate gene names: 197118972

Gene position: 3007124-3005418 (Counterclockwise)

Preceding gene: 197118973

Following gene: 197118971

Centisome position: 65.16

GC content: 61.34

Gene sequence:

>1707_bases
ATGCACGTAAGCAGACTGGGTGAGCTGCTGGTCAGCAACAGCCTCATAACCAAGGAGCAGTTGAAGCAAGCTCTGGCCGA
ACAGAAAGCGGCCGGAGGCCAACTGCGTCTGGGCTCGATACTGGTCAGGGAGAACCTGATCAGCGAGGCCGACCTCACCT
CCTTCCTATCCAAGCAATATGGCGTGCCCACCATAAACCTCGCGGACTACGAGGTTGAGCCCTCGGTGGTTAAGATCATT
CCCGCCGAGATCGCTCACAAATACCAGATCGTACCTGTCAACCGGGCCGGCTCCACGCTGATCATAGCGATGAGCGACCC
GTCCAACATCTTCGCCATCGACGACATCAAGTTCATGACCGGATACAACGTCGAGGTGGTGGTGGTGGCCGAGAGCTCCA
TCAAGGCCGCCATCGACAAGCTCTACGACCAGTCCGCGTCCTTAGCCGATGTGATGAACGACCTGGAGATCGACGACCTC
GAGGTGGTGGGGGACGACGAGGAGGTGGACGTAGGCTCCCTGGAGCGCGCCACCGAGGACGCCCCGGTCGTCAAGCTGGT
GAACCTGATCCTCACCGACGCCATCAAGAAGAAAGCTTCCGATATCCATATCGAGCCCTACGAGAAGTACTTCCGGGTCC
GTTACCGCATCGACGGCGTGCTCTACGAGGTGATGAAGCCCCCCTTGAAGCTGAAAAACGCCATCACCTCCCGCATCAAG
ATCATGAGCGAGCTGGACATCGCCGAAAGGCGACTCCCGCAGGACGGCCGCATCAAGATCAAGCTGGGGGGCGGCAAGGA
CATGGACTTCCGCGTCTCGGTGCTCCCCACCTTGTTCGGCGAGAAGATCGTTCTGCGTCTTCTGGACAAATCGAACCTGC
AGCTCGACATGTCGAAGCTGGGCTACGAGCATGAAGCGCTGGCGCACTTCCAGCGCGAGATCCACAAGCCGTTCGGCATG
GTGCTGGTCACAGGCCCTACCGGCAGCGGCAAGACGGTTTCCCTCTACTCGGCCCTCTCCGAGTTGAACAAGGTCACCGA
GAACATCTCCACCGCCGAGGACCCGGTCGAGTTCAACTTCGCCGGCATCAATCAGGTGCAGATGCACGAGGACATAGGGC
TCAACTTCGCGGCAGCCCTGCGCGCCTTCCTGCGGCAGGACCCCGACGTCATCATGATCGGCGAGATCCGCGATTTCGAG
ACCGCGGAGATCGGCGTGAAAGCAGCGCTCACCGGCCACCTGGTGCTCTCCACGCTGCACACGAACGACGCCCCCTCGAC
CATCAACCGCCTGCTCAACATGGGGATCGAGCCGTTCCTCGTCGCCTCGGCCGTCAACCTCATCTCGGCGCAAAGGCTCG
CGCGCCGGGTCTGCAGCGAATGCAAGCAGGTGGAGGAGGTACCCCCCCAGGCGCTTATCGACGCGGGGCTTCCCCGCGAG
CTGGCCGAATCCGCCGTCTGCTACAGGGGGGCCGGCTGTCCCAAGTGCAACGGGACCGGGTACAAGGGGAGGGTCGGCTT
CTATCAGGTCATGCCGATGCTGGAGCCGATTCGCGAGCTGATACTGAACGGGGCCAACACGGCCGAGATCAAAAGGGAAT
CGATGCGGCTGGGCATCAAGACGATGCGCCAGTCCGGTCTTACCAAGCTGGTGGAGGGGGTCACCTCCTTCGAGGAAGTG
CTGAGGGTGACGGTTGCGGACGACTAA

Upstream 100 bases:

>100_bases
GAAAAGGCGCTAGCCGCCCGGCTCACAACCCCAGGTAACCCTTGACATTACTGTCATTACAAAATACCATTCACAGGTTC
TTTAACCCCCTGAGGTACCT

Downstream 100 bases:

>100_bases
CGAGACGAGGTACTCATGATCAACTTTCACCAAATGCTGAAGGAACTGGTGGAACGTAACGGTTCCGACCTGCACATAAC
CACCAACACCTCGCCGCAGA

Product: type IV pilus biogenesis ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MHVSRLGELLVSNSLITKEQLKQALAEQKAAGGQLRLGSILVRENLISEADLTSFLSKQYGVPTINLADYEVEPSVVKII
PAEIAHKYQIVPVNRAGSTLIIAMSDPSNIFAIDDIKFMTGYNVEVVVVAESSIKAAIDKLYDQSASLADVMNDLEIDDL
EVVGDDEEVDVGSLERATEDAPVVKLVNLILTDAIKKKASDIHIEPYEKYFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMSELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKIVLRLLDKSNLQLDMSKLGYEHEALAHFQREIHKPFGM
VLVTGPTGSGKTVSLYSALSELNKVTENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRAFLRQDPDVIMIGEIRDFE
TAEIGVKAALTGHLVLSTLHTNDAPSTINRLLNMGIEPFLVASAVNLISAQRLARRVCSECKQVEEVPPQALIDAGLPRE
LAESAVCYRGAGCPKCNGTGYKGRVGFYQVMPMLEPIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLVEGVTSFEEV
LRVTVADD

Sequences:

>Translated_568_residues
MHVSRLGELLVSNSLITKEQLKQALAEQKAAGGQLRLGSILVRENLISEADLTSFLSKQYGVPTINLADYEVEPSVVKII
PAEIAHKYQIVPVNRAGSTLIIAMSDPSNIFAIDDIKFMTGYNVEVVVVAESSIKAAIDKLYDQSASLADVMNDLEIDDL
EVVGDDEEVDVGSLERATEDAPVVKLVNLILTDAIKKKASDIHIEPYEKYFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMSELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKIVLRLLDKSNLQLDMSKLGYEHEALAHFQREIHKPFGM
VLVTGPTGSGKTVSLYSALSELNKVTENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRAFLRQDPDVIMIGEIRDFE
TAEIGVKAALTGHLVLSTLHTNDAPSTINRLLNMGIEPFLVASAVNLISAQRLARRVCSECKQVEEVPPQALIDAGLPRE
LAESAVCYRGAGCPKCNGTGYKGRVGFYQVMPMLEPIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLVEGVTSFEEV
LRVTVADD
>Mature_568_residues
MHVSRLGELLVSNSLITKEQLKQALAEQKAAGGQLRLGSILVRENLISEADLTSFLSKQYGVPTINLADYEVEPSVVKII
PAEIAHKYQIVPVNRAGSTLIIAMSDPSNIFAIDDIKFMTGYNVEVVVVAESSIKAAIDKLYDQSASLADVMNDLEIDDL
EVVGDDEEVDVGSLERATEDAPVVKLVNLILTDAIKKKASDIHIEPYEKYFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMSELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKIVLRLLDKSNLQLDMSKLGYEHEALAHFQREIHKPFGM
VLVTGPTGSGKTVSLYSALSELNKVTENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRAFLRQDPDVIMIGEIRDFE
TAEIGVKAALTGHLVLSTLHTNDAPSTINRLLNMGIEPFLVASAVNLISAQRLARRVCSECKQVEEVPPQALIDAGLPRE
LAESAVCYRGAGCPKCNGTGYKGRVGFYQVMPMLEPIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLVEGVTSFEEV
LRVTVADD

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=473, Percent_Identity=42.2832980972516, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=44.9350649350649, Blast_Score=339, Evalue=3e-94,
Organism=Escherichia coli, GI87082188, Length=251, Percent_Identity=33.4661354581673, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62517; Mature: 62517

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVSRLGELLVSNSLITKEQLKQALAEQKAAGGQLRLGSILVRENLISEADLTSFLSKQY
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
GVPTINLADYEVEPSVVKIIPAEIAHKYQIVPVNRAGSTLIIAMSDPSNIFAIDDIKFMT
CCCEEEECCCCCCCHHEEEEEHHHCCCEEEEEECCCCCEEEEEECCCCCEEEEECEEEEC
GYNVEVVVVAESSIKAAIDKLYDQSASLADVMNDLEIDDLEVVGDDEEVDVGSLERATED
CCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCCCCHHHCCCC
APVVKLVNLILTDAIKKKASDIHIEPYEKYFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEHHHHHHHHHHHHCCCHHHHHHHHHHHH
IMSELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKIVLRLLDKSNLQLDMSKL
HHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEHHHC
GYEHEALAHFQREIHKPFGMVLVTGPTGSGKTVSLYSALSELNKVTENISTAEDPVEFNF
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEE
AGINQVQMHEDIGLNFAAALRAFLRQDPDVIMIGEIRDFETAEIGVKAALTGHLVLSTLH
CCCCEEEEHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCHHHHHHHHHHEEECC
TNDAPSTINRLLNMGIEPFLVASAVNLISAQRLARRVCSECKQVEEVPPQALIDAGLPRE
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHH
LAESAVCYRGAGCPKCNGTGYKGRVGFYQVMPMLEPIRELILNGANTAEIKRESMRLGIK
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TMRQSGLTKLVEGVTSFEEVLRVTVADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHVSRLGELLVSNSLITKEQLKQALAEQKAAGGQLRLGSILVRENLISEADLTSFLSKQY
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
GVPTINLADYEVEPSVVKIIPAEIAHKYQIVPVNRAGSTLIIAMSDPSNIFAIDDIKFMT
CCCEEEECCCCCCCHHEEEEEHHHCCCEEEEEECCCCCEEEEEECCCCCEEEEECEEEEC
GYNVEVVVVAESSIKAAIDKLYDQSASLADVMNDLEIDDLEVVGDDEEVDVGSLERATED
CCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCCCCHHHCCCC
APVVKLVNLILTDAIKKKASDIHIEPYEKYFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEHHHHHHHHHHHHCCCHHHHHHHHHHHH
IMSELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKIVLRLLDKSNLQLDMSKL
HHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEHHHC
GYEHEALAHFQREIHKPFGMVLVTGPTGSGKTVSLYSALSELNKVTENISTAEDPVEFNF
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEE
AGINQVQMHEDIGLNFAAALRAFLRQDPDVIMIGEIRDFETAEIGVKAALTGHLVLSTLH
CCCCEEEEHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCHHHHHHHHHHEEECC
TNDAPSTINRLLNMGIEPFLVASAVNLISAQRLARRVCSECKQVEEVPPQALIDAGLPRE
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHH
LAESAVCYRGAGCPKCNGTGYKGRVGFYQVMPMLEPIRELILNGANTAEIKRESMRLGIK
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TMRQSGLTKLVEGVTSFEEVLRVTVADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]