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The map label for this gene is pilT-4 [H]

Identifier: 197118971

GI number: 197118971

Start: 3004302

End: 3005402

Strand: Reverse

Name: pilT-4 [H]

Synonym: Gbem_2594

Alternate gene names: 197118971

Gene position: 3005402-3004302 (Counterclockwise)

Preceding gene: 197118972

Following gene: 197118970

Centisome position: 65.12

GC content: 61.49

Gene sequence:

>1101_bases
ATGATCAACTTTCACCAAATGCTGAAGGAACTGGTGGAACGTAACGGTTCCGACCTGCACATAACCACCAACACCTCGCC
GCAGATCCGCATCGACGGCAAGCTTACCCCGATGGACATCCCGCCTCTGACCCCCATCGAGACCAAGCAGCTCTGCTACA
GCATCCTGACCGACTCGCAAAAGCATAAGTTCGAGGAGGAGAACGAGCTCGACCTCTCCTTCGGCGTCAAGGGGCTATCG
CGCTTCAGGGGGAACATCTTCGTGCAGCGCGGCGCCGTCGCCGGGGTCTTCAGGGTGATCCCCTACAAGATCCTCACCTT
CGAGGAACTGGGGCTTCCGCCGGTAGTGAGGACGCTTTGCGACAAGCCGCGTGGCCTGATCCTCGTTACCGGCCCTACCG
GCAGCGGCAAGTCCACCACACTCGCCTCGATGATCGACCGGATCAACCTGGAGCGCCACGACCACATCGTGACCGTCGAG
GACCCGATCGAGTACCTGCACCCGCACAAGGGATGCATCGTGAACCAGCGCGAGGTCGGGGCGGACACCAAGAGTTTCAA
GCACGCGCTCAAGTACGTGCTGCGCCAGGACCCGGACGTGGTGCTGATCGGGGAGTTGCGCGACCTGGAGACCATCGAGG
CAGCGCTTACCCTCGCCGAAACCGGCCACCTCTGCTTCGCGACCCTGCACACCAACTCCTGCGCCCAGACCATGAACAGG
ATCATCGACGTCTTCCCTCCCTACCAGCAGACCCAGGTGCGCACGCAGCTCTCCTTTGTGCTCGAAGGGGTCATGTCGCA
GACCCTGATACCGAAAGCAAACGGCGGGGGGAGGGCGCTCGCCCTCGAGGTCATGGTTCCCAATCCCGCCATCAGGAACC
TGATCCGCGAGGACAAGGTGCACCAGATCTATTCCCAGATGCAGGTCGGCCAGGAGAAGTTCGGTATGCAGACCATGAAT
CAATGCCTGATGAACCTTTTGGCCAAGCGCATCATAACCGTTGAAGACGCCCTCGGGCGCTCCTCCGAGCCGGACGAACT
GAAGCAGATGCTCGCACCGGGTGCGGGTGCCGGACAGATGAGGCGCCCCCCCCAGAGGTAA

Upstream 100 bases:

>100_bases
GATGCGCCAGTCCGGTCTTACCAAGCTGGTGGAGGGGGTCACCTCCTTCGAGGAAGTGCTGAGGGTGACGGTTGCGGACG
ACTAACGAGACGAGGTACTC

Downstream 100 bases:

>100_bases
GGTTAGCCGGTCCCTTGGCGGGGCTGCAAAGGAGTAAGTAATGCCTAAGTTTGATTGGGAAGCAAGGAGCAAGGCCGGAA
GCACCCAGAAAGGGGTGATG

Product: twitching motility pilus retraction protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MINFHQMLKELVERNGSDLHITTNTSPQIRIDGKLTPMDIPPLTPIETKQLCYSILTDSQKHKFEEENELDLSFGVKGLS
RFRGNIFVQRGAVAGVFRVIPYKILTFEELGLPPVVRTLCDKPRGLILVTGPTGSGKSTTLASMIDRINLERHDHIVTVE
DPIEYLHPHKGCIVNQREVGADTKSFKHALKYVLRQDPDVVLIGELRDLETIEAALTLAETGHLCFATLHTNSCAQTMNR
IIDVFPPYQQTQVRTQLSFVLEGVMSQTLIPKANGGGRALALEVMVPNPAIRNLIREDKVHQIYSQMQVGQEKFGMQTMN
QCLMNLLAKRIITVEDALGRSSEPDELKQMLAPGAGAGQMRRPPQR

Sequences:

>Translated_366_residues
MINFHQMLKELVERNGSDLHITTNTSPQIRIDGKLTPMDIPPLTPIETKQLCYSILTDSQKHKFEEENELDLSFGVKGLS
RFRGNIFVQRGAVAGVFRVIPYKILTFEELGLPPVVRTLCDKPRGLILVTGPTGSGKSTTLASMIDRINLERHDHIVTVE
DPIEYLHPHKGCIVNQREVGADTKSFKHALKYVLRQDPDVVLIGELRDLETIEAALTLAETGHLCFATLHTNSCAQTMNR
IIDVFPPYQQTQVRTQLSFVLEGVMSQTLIPKANGGGRALALEVMVPNPAIRNLIREDKVHQIYSQMQVGQEKFGMQTMN
QCLMNLLAKRIITVEDALGRSSEPDELKQMLAPGAGAGQMRRPPQR
>Mature_366_residues
MINFHQMLKELVERNGSDLHITTNTSPQIRIDGKLTPMDIPPLTPIETKQLCYSILTDSQKHKFEEENELDLSFGVKGLS
RFRGNIFVQRGAVAGVFRVIPYKILTFEELGLPPVVRTLCDKPRGLILVTGPTGSGKSTTLASMIDRINLERHDHIVTVE
DPIEYLHPHKGCIVNQREVGADTKSFKHALKYVLRQDPDVVLIGELRDLETIEAALTLAETGHLCFATLHTNSCAQTMNR
IIDVFPPYQQTQVRTQLSFVLEGVMSQTLIPKANGGGRALALEVMVPNPAIRNLIREDKVHQIYSQMQVGQEKFGMQTMN
QCLMNLLAKRIITVEDALGRSSEPDELKQMLAPGAGAGQMRRPPQR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=41.4330218068536, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1786296, Length=279, Percent_Identity=35.1254480286738, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789723, Length=286, Percent_Identity=32.1678321678322, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40907; Mature: 40907

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINFHQMLKELVERNGSDLHITTNTSPQIRIDGKLTPMDIPPLTPIETKQLCYSILTDSQ
CCCHHHHHHHHHHCCCCEEEEEECCCCEEEECCEECCCCCCCCCCCCHHHHHHHHHCCCH
KHKFEEENELDLSFGVKGLSRFRGNIFVQRGAVAGVFRVIPYKILTFEELGLPPVVRTLC
HHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHEEHHHCCCCHHHHHHC
DKPRGLILVTGPTGSGKSTTLASMIDRINLERHDHIVTVEDPIEYLHPHKGCIVNQREVG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCCCCEECHHHCC
ADTKSFKHALKYVLRQDPDVVLIGELRDLETIEAALTLAETGHLCFATLHTNSCAQTMNR
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
IIDVFPPYQQTQVRTQLSFVLEGVMSQTLIPKANGGGRALALEVMVPNPAIRNLIREDKV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HQIYSQMQVGQEKFGMQTMNQCLMNLLAKRIITVEDALGRSSEPDELKQMLAPGAGAGQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCC
RRPPQR
CCCCCC
>Mature Secondary Structure
MINFHQMLKELVERNGSDLHITTNTSPQIRIDGKLTPMDIPPLTPIETKQLCYSILTDSQ
CCCHHHHHHHHHHCCCCEEEEEECCCCEEEECCEECCCCCCCCCCCCHHHHHHHHHCCCH
KHKFEEENELDLSFGVKGLSRFRGNIFVQRGAVAGVFRVIPYKILTFEELGLPPVVRTLC
HHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHEEHHHCCCCHHHHHHC
DKPRGLILVTGPTGSGKSTTLASMIDRINLERHDHIVTVEDPIEYLHPHKGCIVNQREVG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCCCCEECHHHCC
ADTKSFKHALKYVLRQDPDVVLIGELRDLETIEAALTLAETGHLCFATLHTNSCAQTMNR
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
IIDVFPPYQQTQVRTQLSFVLEGVMSQTLIPKANGGGRALALEVMVPNPAIRNLIREDKV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HQIYSQMQVGQEKFGMQTMNQCLMNLLAKRIITVEDALGRSSEPDELKQMLAPGAGAGQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCC
RRPPQR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]