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The map label for this gene is rfbE [H]

Identifier: 197118954

GI number: 197118954

Start: 2986668

End: 2987765

Strand: Reverse

Name: rfbE [H]

Synonym: Gbem_2577

Alternate gene names: 197118954

Gene position: 2987765-2986668 (Counterclockwise)

Preceding gene: 197118955

Following gene: 197118953

Centisome position: 64.74

GC content: 59.02

Gene sequence:

>1098_bases
ATGACTTTTCAGATTCCTATTTACCAACCGTCGCTGCTGGGTAACGAAAAGAAGTACGTCAACGAATGCCTGGATTCCAC
CTGGATCTCATCCAAGGGGCGCTTCATCGCAGAATTCGAAGAGAAATTCGCCAATTACACCGGCGCGCGCTACGCCGCTT
CGGTCTGTAACGGCACGGTGGCGCTGCACCTCGCCTTGGTGGCGCTAGGCATCGGTCCGGGGGACGAGGTGATTGTCCCC
ACTCTGACCTACATCTCCTCGGTCAACGCCATCACCTATACCGGAGCCACGCCCGTTTTTGTCGATGCCCTGGAGAGCAC
CTGGCAGATGGACCCGGATGACGTGAGGCGCAAGATTACACCCCGTACGCGCGCCATCATGGTGGTGCATCTCTACGGCC
ACCCCTGCGACATGGACCAACTGGTCGACATTGCCAAAGGGCGCGGGCTCTTCATGATAGAGGACTGCGCCGAGGCCTTT
GGTGCTCGTTACAAGGGGCGGCACGTCGGTAGCTTCGGCGATATCTCCACCTACAGCTTTTTCGGCAACAAGACCATCAC
CACCGGCGAGGGCGGGATGGTGGTGACCAATGACGAGACCCTCCATGACCGGGCAGTGCATTTCAAGGGGCAGGGGCTGG
CAAAACACCGGGAATACTGGCACGATGTAGTCGGGTACAACTACCGCATGACAAACATTTGCGCCGCCCTCGGTCTCGCG
CAGTTGGAGCGCTCTGACGAACTGCTGGATAAGAAAAGGGAGGTAGCCCGGTGGTATGCCGAGGAACTCGAAGGAGCGCC
GGTCCTCTTGCACCGAGAAACCGCGGACGTGCGGCATTCCTACTGGATGTGCTCGCTTCAGGTTCTTGATCCGGCCTTGC
GGGAGCCGCTGCGTCTTGCTCTTGGCAACGCCGGGATCGAAACGCGTCCCGTTTTCTATCCGCTGCACACCATGCCGATG
TACGCCCAGAAATTCCAGCGACACCCGGTTTCCGAGCGCATCGGTTGGCGCGGTGTCTGCCTGCCGAGTTGGCCGGAGAT
TTCCAAAGACCAGGTGCAGTTCATCTGCGGTGAGATCAACGGATTCCTCAATGGCTGA

Upstream 100 bases:

>100_bases
CAGGGGAATCTGACCTTCATCCCGGGCCATGACGCGGTCGAATCCATTCGCGGCATGCAGGTTTTCAACACCACGACGCA
GACCATAGAGGAACTTTGAC

Downstream 100 bases:

>100_bases
GCATTCAGCAAAGAAGAGGCACTTGTTCATACTTGGGTGCGGCGGCCATAGCCGAAGCGTCACCGACGTGATTTTGCGCG
GAGACCCTGACGCCGTTCTT

Product: glutamate--GDP-4-keto-6-deoxy-D-mannose aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTFQIPIYQPSLLGNEKKYVNECLDSTWISSKGRFIAEFEEKFANYTGARYAASVCNGTVALHLALVALGIGPGDEVIVP
TLTYISSVNAITYTGATPVFVDALESTWQMDPDDVRRKITPRTRAIMVVHLYGHPCDMDQLVDIAKGRGLFMIEDCAEAF
GARYKGRHVGSFGDISTYSFFGNKTITTGEGGMVVTNDETLHDRAVHFKGQGLAKHREYWHDVVGYNYRMTNICAALGLA
QLERSDELLDKKREVARWYAEELEGAPVLLHRETADVRHSYWMCSLQVLDPALREPLRLALGNAGIETRPVFYPLHTMPM
YAQKFQRHPVSERIGWRGVCLPSWPEISKDQVQFICGEINGFLNG

Sequences:

>Translated_365_residues
MTFQIPIYQPSLLGNEKKYVNECLDSTWISSKGRFIAEFEEKFANYTGARYAASVCNGTVALHLALVALGIGPGDEVIVP
TLTYISSVNAITYTGATPVFVDALESTWQMDPDDVRRKITPRTRAIMVVHLYGHPCDMDQLVDIAKGRGLFMIEDCAEAF
GARYKGRHVGSFGDISTYSFFGNKTITTGEGGMVVTNDETLHDRAVHFKGQGLAKHREYWHDVVGYNYRMTNICAALGLA
QLERSDELLDKKREVARWYAEELEGAPVLLHRETADVRHSYWMCSLQVLDPALREPLRLALGNAGIETRPVFYPLHTMPM
YAQKFQRHPVSERIGWRGVCLPSWPEISKDQVQFICGEINGFLNG
>Mature_364_residues
TFQIPIYQPSLLGNEKKYVNECLDSTWISSKGRFIAEFEEKFANYTGARYAASVCNGTVALHLALVALGIGPGDEVIVPT
LTYISSVNAITYTGATPVFVDALESTWQMDPDDVRRKITPRTRAIMVVHLYGHPCDMDQLVDIAKGRGLFMIEDCAEAFG
ARYKGRHVGSFGDISTYSFFGNKTITTGEGGMVVTNDETLHDRAVHFKGQGLAKHREYWHDVVGYNYRMTNICAALGLAQ
LERSDELLDKKREVARWYAEELEGAPVLLHRETADVRHSYWMCSLQVLDPALREPLRLALGNAGIETRPVFYPLHTMPMY
AQKFQRHPVSERIGWRGVCLPSWPEISKDQVQFICGEINGFLNG

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=364, Percent_Identity=35.7142857142857, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI2367285, Length=263, Percent_Identity=33.0798479087452, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 41007; Mature: 40876

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQIPIYQPSLLGNEKKYVNECLDSTWISSKGRFIAEFEEKFANYTGARYAASVCNGTV
CEEEECCCCCHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHH
ALHLALVALGIGPGDEVIVPTLTYISSVNAITYTGATPVFVDALESTWQMDPDDVRRKIT
HHHHHHHHHCCCCCCCEEEHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHCC
PRTRAIMVVHLYGHPCDMDQLVDIAKGRGLFMIEDCAEAFGARYKGRHVGSFGDISTYSF
CCCEEEEEEEEECCCCCHHHHHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCEEEE
FGNKTITTGEGGMVVTNDETLHDRAVHFKGQGLAKHREYWHDVVGYNYRMTNICAALGLA
ECCCEEEECCCCEEEECCCCHHHHEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLERSDELLDKKREVARWYAEELEGAPVLLHRETADVRHSYWMCSLQVLDPALREPLRLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHEEEHHHHCHHHHHHHHHH
LGNAGIETRPVFYPLHTMPMYAQKFQRHPVSERIGWRGVCLPSWPEISKDQVQFICGEIN
HCCCCCCCCCEEEECCCCHHHHHHHHCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHH
GFLNG
CCCCC
>Mature Secondary Structure 
TFQIPIYQPSLLGNEKKYVNECLDSTWISSKGRFIAEFEEKFANYTGARYAASVCNGTV
EEEECCCCCHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHH
ALHLALVALGIGPGDEVIVPTLTYISSVNAITYTGATPVFVDALESTWQMDPDDVRRKIT
HHHHHHHHHCCCCCCCEEEHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHCC
PRTRAIMVVHLYGHPCDMDQLVDIAKGRGLFMIEDCAEAFGARYKGRHVGSFGDISTYSF
CCCEEEEEEEEECCCCCHHHHHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCEEEE
FGNKTITTGEGGMVVTNDETLHDRAVHFKGQGLAKHREYWHDVVGYNYRMTNICAALGLA
ECCCEEEECCCCEEEECCCCHHHHEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLERSDELLDKKREVARWYAEELEGAPVLLHRETADVRHSYWMCSLQVLDPALREPLRLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHEEEHHHHCHHHHHHHHHH
LGNAGIETRPVFYPLHTMPMYAQKFQRHPVSERIGWRGVCLPSWPEISKDQVQFICGEIN
HCCCCCCCCCEEEECCCCHHHHHHHHCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHH
GFLNG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]