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The map label for this gene is epsM [H]

Identifier: 197118953

GI number: 197118953

Start: 2986055

End: 2986675

Strand: Reverse

Name: epsM [H]

Synonym: Gbem_2576

Alternate gene names: 197118953

Gene position: 2986675-2986055 (Counterclockwise)

Preceding gene: 197118954

Following gene: 197118952

Centisome position: 64.71

GC content: 56.84

Gene sequence:

>621_bases
ATGGCTGAGCATTCAGCAAAGAAGAGGCACTTGTTCATACTTGGGTGCGGCGGCCATAGCCGAAGCGTCACCGACGTGAT
TTTGCGCGGAGACCCTGACGCCGTTCTTGTTTTCGTCGACGAAAACGCCCGGGAAAACGAGACCCTCTACGGGTTCGAGA
TGACCTCCAACTTAGCTGATGTGCAAGGCCCATGGTTCCCTGCCATAGGGGACAACGAAAAAAGAAAGGCCCTCATGTCG
CGGCTTGACGCCAGGGACATGGTCTCCGTTGTTTCTGTGAACGCCCATATCGGGTTTAAGGCCCGCATCGGACGCGGCTG
CTTTGTCGGGCACTTTTGTCACGTAGGCCCCGAGGCTGTCATTGGCGAGGGGACCATTGTTAATACGGCATCGGTCGTCG
AGCATGAGGTTCAGATCGGTAGTTATTGCCACGTAGGTCCCAATGCGACGGTCTCCGGCAGATGCAAAATCGGCGACCTC
GTCTTTGTCGGCGTCGGCGCTACCATCAAGGACTACATCTCCATCTGTTCCGGCGTGGTCGTCGGCGCAGGCGCCACGGT
AGTCAGAGATATCACTGAGCCAGGCGTCTATGTCGGCTGTCCGGCTGTAAGGATCAAATGA

Upstream 100 bases:

>100_bases
TCCGAGCGCATCGGTTGGCGCGGTGTCTGCCTGCCGAGTTGGCCGGAGATTTCCAAAGACCAGGTGCAGTTCATCTGCGG
TGAGATCAACGGATTCCTCA

Downstream 100 bases:

>100_bases
GAACCAAGCTTTCCATCGCGCTCTTGGGGCGCAGTTTCGACTGGGGGGGCGGCATGGACTTTCTACGCCACCTCGCCAAC
GGACTGCTTTTCAAGCAGAA

Product: acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MAEHSAKKRHLFILGCGGHSRSVTDVILRGDPDAVLVFVDENARENETLYGFEMTSNLADVQGPWFPAIGDNEKRKALMS
RLDARDMVSVVSVNAHIGFKARIGRGCFVGHFCHVGPEAVIGEGTIVNTASVVEHEVQIGSYCHVGPNATVSGRCKIGDL
VFVGVGATIKDYISICSGVVVGAGATVVRDITEPGVYVGCPAVRIK

Sequences:

>Translated_206_residues
MAEHSAKKRHLFILGCGGHSRSVTDVILRGDPDAVLVFVDENARENETLYGFEMTSNLADVQGPWFPAIGDNEKRKALMS
RLDARDMVSVVSVNAHIGFKARIGRGCFVGHFCHVGPEAVIGEGTIVNTASVVEHEVQIGSYCHVGPNATVSGRCKIGDL
VFVGVGATIKDYISICSGVVVGAGATVVRDITEPGVYVGCPAVRIK
>Mature_205_residues
AEHSAKKRHLFILGCGGHSRSVTDVILRGDPDAVLVFVDENARENETLYGFEMTSNLADVQGPWFPAIGDNEKRKALMSR
LDARDMVSVVSVNAHIGFKARIGRGCFVGHFCHVGPEAVIGEGTIVNTASVVEHEVQIGSYCHVGPNATVSGRCKIGDLV
FVGVGATIKDYISICSGVVVGAGATVVRDITEPGVYVGCPAVRIK

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 21865; Mature: 21734

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00101 HEXAPEP_TRANSFERASES ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHSAKKRHLFILGCGGHSRSVTDVILRGDPDAVLVFVDENARENETLYGFEMTSNLAD
CCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEEEEECCCCCC
VQGPWFPAIGDNEKRKALMSRLDARDMVSVVSVNAHIGFKARIGRGCFVGHFCHVGPEAV
CCCCCCCCCCCCHHHHHHHHHCCHHHHEEEEEEEEECCEEEECCCCEEEEHEECCCCCCE
IGEGTIVNTASVVEHEVQIGSYCHVGPNATVSGRCKIGDLVFVGVGATIKDYISICSGVV
ECCCEEEEHHHHHHHHEEECCEEECCCCCEEECCEEECCEEEEECCCCHHHHHHHHCCEE
VGAGATVVRDITEPGVYVGCPAVRIK
ECCCCCEEECCCCCCEEECCCEEEEC
>Mature Secondary Structure 
AEHSAKKRHLFILGCGGHSRSVTDVILRGDPDAVLVFVDENARENETLYGFEMTSNLAD
CCCCCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEEEEECCCCCC
VQGPWFPAIGDNEKRKALMSRLDARDMVSVVSVNAHIGFKARIGRGCFVGHFCHVGPEAV
CCCCCCCCCCCCHHHHHHHHHCCHHHHEEEEEEEEECCEEEECCCCEEEEHEECCCCCCE
IGEGTIVNTASVVEHEVQIGSYCHVGPNATVSGRCKIGDLVFVGVGATIKDYISICSGVV
ECCCEEEEHHHHHHHHEEECCEEECCCCCEEECCEEECCEEEEECCCCHHHHHHHHCCEE
VGAGATVVRDITEPGVYVGCPAVRIK
ECCCCCEEECCCCCCEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]