Click here to switch to the map view.

The map label for this gene is emrY [H]

Identifier: 197118904

GI number: 197118904

Start: 2929115

End: 2930683

Strand: Direct

Name: emrY [H]

Synonym: Gbem_2527

Alternate gene names: 197118904

Gene position: 2929115-2930683 (Clockwise)

Preceding gene: 197118903

Following gene: 197118905

Centisome position: 63.47

GC content: 53.22

Gene sequence:

>1569_bases
ATGAGCGCGAATCAGGCTACGCCCGCTCCCCCACCTGCCGGCAGCATGCCAGCGGCTCCGTTCAATGGCCGTCTGCTCAC
CGGTTTTATAGGTATCGTGATTTCCGCCATGATGGCGGGTCTCAGCAACCGCGTCGGTGCACTGGCCTTGGTCGATATTC
GCGGTGTACTCGGCCTAGGGGTAGACGAGGCGAGTTGGCTTAATACCGTTTACATAGCTGCGGAATTGATCGCCATGCCT
TTCTCTATCTGGTTGTCAACGATCATGTCCCTGCGGCGCTTCCACATGCTGGTAACCGGCTTATTCGTTCTGCTTTCTTT
TGTTATTCCCTGGATACACGACTGGTCAGCCTTGTTGATGTTGCGCAGTCTTCAAGGGTTGGCGGGCGGCATGCTGATAC
CAATTCTGATGGTGGCAGCACTACGTTTTTTCCCTTTGCCGGTTCGCCTGTATGGCATGTCGCTATATGCCCTGACGGCT
ACTTTCTCGCCCAATCTCGGATTCTGGCTGGCCGGCACCTGGGTCGATATCTTGTTTGATTGGCGGCTGATTTATTGGCA
AAACCTGCCTCTGGGACTGCTGGCGATCGTGATGGTGTATTGGGGAATTCCTCAGGATCCGACCAGACCGGAACGCTTTA
AACAGACCGATTGGTTCGGCATGGTCTGTGGCGTGATTGGGTTAGGAATGATCGCTATCGGGCTAGACCAGGGCGAGCGA
ATGGATTGGTGGCGCTCGTACTTGATCTGCTGGCTGATTGGCGGTGGTATATCAATGACAGTGGTTTTCCTTATCTGTCA
GTGGTATTACCCGATCCCATTCATAAAGCCGCAGTTGCTTGTACAAAGACGAAATCTAGGAATGGGCTTCTCAATTTTCG
TTTTCCTGCTGATGGTCATGTTGTCCAGTTCAAAACTGCCGGCGATGCATCTTGAAGCCCTATGGGGCTACCGCAGTCTG
CAAAACAGCCCAATCGGGCTGCTCATTGCAGTGCCTCAACCGATTCTATCGCTGGGGGTAGCCTTATTGCTGTACCGAAA
ATGGGTAGATGCCCGCATTGTGATGGCATCTGGAATAGCTTTAATCGCTGCTGCTTGCCTTATCTGCACACATATCACCT
CCAGCTGGATTATTGAACAACTGGTGCTGGCCCAAGTTTTACAGGCCGTCGGGCAGGCCATGGCCGTGGTTTGCCTGTTG
TTCCTGACGACCAGCGTTGTGCAACCGATGGAAGGTCCATATGTGGCCGGCACCATCAATACCCTGCGCGCCTTCGGAGT
AATTTTCGGTGGTGCTGTGATCGGTCATTTCTTCCGTCTGCGTCAGCATTTTCATTCGGAGATGCTGGTGGACCGCATCG
GCCTGATTTCCGCGAGTATTCCGCTATCCCAAGACAACCTTGCCGCATTAACCGACAGTGCTCGTCAGCAGGCTTTTGTT
TTGGCCAATGCTGACAGCTATCGGATGCTCGCCGCGCTGGCGGTGCTGCTCATACCTTTCGTATTAAGAATGGATTACAT
AGCGGCTCCGGCCAGGCAAACCTCAGCACCTATCACAAAGAAAGGATAA

Upstream 100 bases:

>100_bases
TTGCGGACAACACCAGCCGAGTTTCGTTAGCCATGGTCAACCTGTACAAAGCGCTGGGGGGGGGATGGGCAGCCCAGACG
GATGAAAAGGGAGGGCAGCG

Downstream 100 bases:

>100_bases
GACAATGGCATTTCCAAAAACAGCAATGATCATCTCCGGCCTCGTTTTAGTGTCCGTAGTCGGAGCGGGTATTTACCTTC
TCAATCGTCACCAATCCGAT

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSANQATPAPPPAGSMPAAPFNGRLLTGFIGIVISAMMAGLSNRVGALALVDIRGVLGLGVDEASWLNTVYIAAELIAMP
FSIWLSTIMSLRRFHMLVTGLFVLLSFVIPWIHDWSALLMLRSLQGLAGGMLIPILMVAALRFFPLPVRLYGMSLYALTA
TFSPNLGFWLAGTWVDILFDWRLIYWQNLPLGLLAIVMVYWGIPQDPTRPERFKQTDWFGMVCGVIGLGMIAIGLDQGER
MDWWRSYLICWLIGGGISMTVVFLICQWYYPIPFIKPQLLVQRRNLGMGFSIFVFLLMVMLSSSKLPAMHLEALWGYRSL
QNSPIGLLIAVPQPILSLGVALLLYRKWVDARIVMASGIALIAAACLICTHITSSWIIEQLVLAQVLQAVGQAMAVVCLL
FLTTSVVQPMEGPYVAGTINTLRAFGVIFGGAVIGHFFRLRQHFHSEMLVDRIGLISASIPLSQDNLAALTDSARQQAFV
LANADSYRMLAALAVLLIPFVLRMDYIAAPARQTSAPITKKG

Sequences:

>Translated_522_residues
MSANQATPAPPPAGSMPAAPFNGRLLTGFIGIVISAMMAGLSNRVGALALVDIRGVLGLGVDEASWLNTVYIAAELIAMP
FSIWLSTIMSLRRFHMLVTGLFVLLSFVIPWIHDWSALLMLRSLQGLAGGMLIPILMVAALRFFPLPVRLYGMSLYALTA
TFSPNLGFWLAGTWVDILFDWRLIYWQNLPLGLLAIVMVYWGIPQDPTRPERFKQTDWFGMVCGVIGLGMIAIGLDQGER
MDWWRSYLICWLIGGGISMTVVFLICQWYYPIPFIKPQLLVQRRNLGMGFSIFVFLLMVMLSSSKLPAMHLEALWGYRSL
QNSPIGLLIAVPQPILSLGVALLLYRKWVDARIVMASGIALIAAACLICTHITSSWIIEQLVLAQVLQAVGQAMAVVCLL
FLTTSVVQPMEGPYVAGTINTLRAFGVIFGGAVIGHFFRLRQHFHSEMLVDRIGLISASIPLSQDNLAALTDSARQQAFV
LANADSYRMLAALAVLLIPFVLRMDYIAAPARQTSAPITKKG
>Mature_521_residues
SANQATPAPPPAGSMPAAPFNGRLLTGFIGIVISAMMAGLSNRVGALALVDIRGVLGLGVDEASWLNTVYIAAELIAMPF
SIWLSTIMSLRRFHMLVTGLFVLLSFVIPWIHDWSALLMLRSLQGLAGGMLIPILMVAALRFFPLPVRLYGMSLYALTAT
FSPNLGFWLAGTWVDILFDWRLIYWQNLPLGLLAIVMVYWGIPQDPTRPERFKQTDWFGMVCGVIGLGMIAIGLDQGERM
DWWRSYLICWLIGGGISMTVVFLICQWYYPIPFIKPQLLVQRRNLGMGFSIFVFLLMVMLSSSKLPAMHLEALWGYRSLQ
NSPIGLLIAVPQPILSLGVALLLYRKWVDARIVMASGIALIAAACLICTHITSSWIIEQLVLAQVLQAVGQAMAVVCLLF
LTTSVVQPMEGPYVAGTINTLRAFGVIFGGAVIGHFFRLRQHFHSEMLVDRIGLISASIPLSQDNLAALTDSARQQAFVL
ANADSYRMLAALAVLLIPFVLRMDYIAAPARQTSAPITKKG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=541, Percent_Identity=22.735674676525, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789042, Length=341, Percent_Identity=23.1671554252199, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57389; Mature: 57257

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANQATPAPPPAGSMPAAPFNGRLLTGFIGIVISAMMAGLSNRVGALALVDIRGVLGLG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCC
VDEASWLNTVYIAAELIAMPFSIWLSTIMSLRRFHMLVTGLFVLLSFVIPWIHDWSALLM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRSLQGLAGGMLIPILMVAALRFFPLPVRLYGMSLYALTATFSPNLGFWLAGTWVDILFD
HHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHEEHEEEECCCCCCHHHHHHHHHHHHH
WRLIYWQNLPLGLLAIVMVYWGIPQDPTRPERFKQTDWFGMVCGVIGLGMIAIGLDQGER
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
MDWWRSYLICWLIGGGISMTVVFLICQWYYPIPFIKPQLLVQRRNLGMGFSIFVFLLMVM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
LSSSKLPAMHLEALWGYRSLQNSPIGLLIAVPQPILSLGVALLLYRKWVDARIVMASGIA
HCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LIAAACLICTHITSSWIIEQLVLAQVLQAVGQAMAVVCLLFLTTSVVQPMEGPYVAGTIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
TLRAFGVIFGGAVIGHFFRLRQHFHSEMLVDRIGLISASIPLSQDNLAALTDSARQQAFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCEEE
LANADSYRMLAALAVLLIPFVLRMDYIAAPARQTSAPITKKG
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SANQATPAPPPAGSMPAAPFNGRLLTGFIGIVISAMMAGLSNRVGALALVDIRGVLGLG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCC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EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]