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The map label for this gene is oprM [H]

Identifier: 197118903

GI number: 197118903

Start: 2927604

End: 2929118

Strand: Direct

Name: oprM [H]

Synonym: Gbem_2526

Alternate gene names: 197118903

Gene position: 2927604-2929118 (Clockwise)

Preceding gene: 197118902

Following gene: 197118904

Centisome position: 63.43

GC content: 61.06

Gene sequence:

>1515_bases
ATGCGGTTTGGAAATGAAAACGACTTTGACATGACTGGAGAGTTCGCAAACCGGCCGGATGCACGAAAATACATCCGCCA
CTTGCCCGTGTTCCTGAGCCTTGCCTTCCTGGGCGCATGTGCCGTCGGCCCTGATTTCAAGAGGCCGGTGAGCACGCCTC
CCGCGCACCTCATGGAGACCACCGGCGAAACCGGCAGGCAGAGCCAGGTAGTGACGGGGAAGATGGAGACGGCATGGTGG
ACGCTGTTCAACGACGAGATTCTGAGCGAACTTCAGGAGCGCGCTCAGAGAGGGAACTTCGATCTGCAGTTGGCGGCTGC
GCGTCTGGCGCAAAGCCGTGCGCAGACCGGCATAGCTAATTCTGCAGGGCTTCCGCATCTCAGCATAGGCGGCTCCTATG
CGCGGGCCGCAGACAGCGCTAACGGCCAAATGGCGCTACTTGGCGCTCCCGACACCCCCTACGACATTATGCAGGCGGGA
TTCAACGCGAGTTGGGAAATCGACATGTGGGGGCACGCCCGGAGGACCAGCGAGTCTGCCGCGGCATCGCTGCAGGCAAG
CGCGTTCCAACGAGAAGGGGTGCGCGTGTCGCTTGCCGCCGAGGTAGCCCGCACCTACATCCAGTTGCGCCACGTCCAGA
CGCAATTGGACATTACCCGCCAAAACCTGGCCATTGCCGAGAATGCCCTGAAGCTGGCGGACAGTCGTGTAAAAAACGGC
ATAGCCACCCGCTTCGAGTCGGCAGCATCATCAGCACAGTTGGCGTCGACCAAGGCGCTGATCCCTCGCCTGGAGGAGCA
ACGTGACGCGCTGATGAATGCCCTCGCCTTGCTGCTTGGCGAACCTCCTCGTACCCTGGATGCCATGTTGACACCGACGC
ACCAAATCCCGCCCGTGCCAAGCGAGGTGCCAATCGGTTTATCCTCTGAACTTGCTCGCCGTCGCCCCGACATCCTCCAA
GCCGAAGCACGTCTTCATGCTGCAACAGCTGCCATCGGTGCAGCCAAAGCTGACTTTTATCCGCGCATCAGCCTGAACGG
CAGCCTTGGTTTCCAGTCCCTCAACCTGGATACCTTCGGCAACTGGGCATCGCGCACCTTCGCGGTGGGACCGACTCTCT
ACCTGCCCATCTTCGAAGGGGGGAGGCTGAGAGGCACGCTTGCCCTTAGCGAGGCCCGTCAACAGGAGGCGGTGATCGCC
TATCAGAAAACGGTGCTGCATGCCTGGCACGAGGTCGACGATGCGTTGAGCTCTTATTCCGAGGTACAGCGGCGCGGCGA
ACAACTGGCCCTGGCCTTCGGCCAATACCGCCAGGCTTACCAAGTTGCGCTGCGTCGCTATGAGCAGGGTGCCGCCGAGT
ACCTGATGGTCCTGACCGCACAACGCTCGTTATTGGCCAGCCAGATGGAACTTGCGGACAACACCAGCCGAGTTTCGTTA
GCCATGGTCAACCTGTACAAAGCGCTGGGGGGGGGATGGGCAGCCCAGACGGATGAAAAGGGAGGGCAGCGATGA

Upstream 100 bases:

>100_bases
TCTTTTATATCAAGTCGCCTATCGAATTCGGCACAACAGGGAATCGGTAAGCGATCCGACGGCTTTACAAGCTTCGTTTA
ACACTTGTCTGGGGTAGATC

Downstream 100 bases:

>100_bases
GCGCGAATCAGGCTACGCCCGCTCCCCCACCTGCCGGCAGCATGCCAGCGGCTCCGTTCAATGGCCGTCTGCTCACCGGT
TTTATAGGTATCGTGATTTC

Product: RND family efflux pump outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MRFGNENDFDMTGEFANRPDARKYIRHLPVFLSLAFLGACAVGPDFKRPVSTPPAHLMETTGETGRQSQVVTGKMETAWW
TLFNDEILSELQERAQRGNFDLQLAAARLAQSRAQTGIANSAGLPHLSIGGSYARAADSANGQMALLGAPDTPYDIMQAG
FNASWEIDMWGHARRTSESAAASLQASAFQREGVRVSLAAEVARTYIQLRHVQTQLDITRQNLAIAENALKLADSRVKNG
IATRFESAASSAQLASTKALIPRLEEQRDALMNALALLLGEPPRTLDAMLTPTHQIPPVPSEVPIGLSSELARRRPDILQ
AEARLHAATAAIGAAKADFYPRISLNGSLGFQSLNLDTFGNWASRTFAVGPTLYLPIFEGGRLRGTLALSEARQQEAVIA
YQKTVLHAWHEVDDALSSYSEVQRRGEQLALAFGQYRQAYQVALRRYEQGAAEYLMVLTAQRSLLASQMELADNTSRVSL
AMVNLYKALGGGWAAQTDEKGGQR

Sequences:

>Translated_504_residues
MRFGNENDFDMTGEFANRPDARKYIRHLPVFLSLAFLGACAVGPDFKRPVSTPPAHLMETTGETGRQSQVVTGKMETAWW
TLFNDEILSELQERAQRGNFDLQLAAARLAQSRAQTGIANSAGLPHLSIGGSYARAADSANGQMALLGAPDTPYDIMQAG
FNASWEIDMWGHARRTSESAAASLQASAFQREGVRVSLAAEVARTYIQLRHVQTQLDITRQNLAIAENALKLADSRVKNG
IATRFESAASSAQLASTKALIPRLEEQRDALMNALALLLGEPPRTLDAMLTPTHQIPPVPSEVPIGLSSELARRRPDILQ
AEARLHAATAAIGAAKADFYPRISLNGSLGFQSLNLDTFGNWASRTFAVGPTLYLPIFEGGRLRGTLALSEARQQEAVIA
YQKTVLHAWHEVDDALSSYSEVQRRGEQLALAFGQYRQAYQVALRRYEQGAAEYLMVLTAQRSLLASQMELADNTSRVSL
AMVNLYKALGGGWAAQTDEKGGQR
>Mature_504_residues
MRFGNENDFDMTGEFANRPDARKYIRHLPVFLSLAFLGACAVGPDFKRPVSTPPAHLMETTGETGRQSQVVTGKMETAWW
TLFNDEILSELQERAQRGNFDLQLAAARLAQSRAQTGIANSAGLPHLSIGGSYARAADSANGQMALLGAPDTPYDIMQAG
FNASWEIDMWGHARRTSESAAASLQASAFQREGVRVSLAAEVARTYIQLRHVQTQLDITRQNLAIAENALKLADSRVKNG
IATRFESAASSAQLASTKALIPRLEEQRDALMNALALLLGEPPRTLDAMLTPTHQIPPVPSEVPIGLSSELARRRPDILQ
AEARLHAATAAIGAAKADFYPRISLNGSLGFQSLNLDTFGNWASRTFAVGPTLYLPIFEGGRLRGTLALSEARQQEAVIA
YQKTVLHAWHEVDDALSSYSEVQRRGEQLALAFGQYRQAYQVALRRYEQGAAEYLMVLTAQRSLLASQMELADNTSRVSL
AMVNLYKALGGGWAAQTDEKGGQR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=472, Percent_Identity=29.4491525423729, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI2367348, Length=431, Percent_Identity=26.9141531322506, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54972; Mature: 54972

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFGNENDFDMTGEFANRPDARKYIRHLPVFLSLAFLGACAVGPDFKRPVSTPPAHLMET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
TGETGRQSQVVTGKMETAWWTLFNDEILSELQERAQRGNFDLQLAAARLAQSRAQTGIAN
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
SAGLPHLSIGGSYARAADSANGQMALLGAPDTPYDIMQAGFNASWEIDMWGHARRTSESA
CCCCCEEECCCCHHHHCCCCCCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCHHCCHHH
AASLQASAFQREGVRVSLAAEVARTYIQLRHVQTQLDITRQNLAIAENALKLADSRVKNG
HHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IATRFESAASSAQLASTKALIPRLEEQRDALMNALALLLGEPPRTLDAMLTPTHQIPPVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
SEVPIGLSSELARRRPDILQAEARLHAATAAIGAAKADFYPRISLNGSLGFQSLNLDTFG
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEECCCCCC
NWASRTFAVGPTLYLPIFEGGRLRGTLALSEARQQEAVIAYQKTVLHAWHEVDDALSSYS
CCCCCEEEECCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVQRRGEQLALAFGQYRQAYQVALRRYEQGAAEYLMVLTAQRSLLASQMELADNTSRVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AMVNLYKALGGGWAAQTDEKGGQR
HHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRFGNENDFDMTGEFANRPDARKYIRHLPVFLSLAFLGACAVGPDFKRPVSTPPAHLMET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
TGETGRQSQVVTGKMETAWWTLFNDEILSELQERAQRGNFDLQLAAARLAQSRAQTGIAN
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
SAGLPHLSIGGSYARAADSANGQMALLGAPDTPYDIMQAGFNASWEIDMWGHARRTSESA
CCCCCEEECCCCHHHHCCCCCCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCHHCCHHH
AASLQASAFQREGVRVSLAAEVARTYIQLRHVQTQLDITRQNLAIAENALKLADSRVKNG
HHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IATRFESAASSAQLASTKALIPRLEEQRDALMNALALLLGEPPRTLDAMLTPTHQIPPVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
SEVPIGLSSELARRRPDILQAEARLHAATAAIGAAKADFYPRISLNGSLGFQSLNLDTFG
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEECCCCCC
NWASRTFAVGPTLYLPIFEGGRLRGTLALSEARQQEAVIAYQKTVLHAWHEVDDALSSYS
CCCCCEEEECCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVQRRGEQLALAFGQYRQAYQVALRRYEQGAAEYLMVLTAQRSLLASQMELADNTSRVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AMVNLYKALGGGWAAQTDEKGGQR
HHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]