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The map label for this gene is entA [C]

Identifier: 197118870

GI number: 197118870

Start: 2887899

End: 2888501

Strand: Direct

Name: entA [C]

Synonym: Gbem_2493

Alternate gene names: 197118870

Gene position: 2887899-2888501 (Clockwise)

Preceding gene: 197118869

Following gene: 197118880

Centisome position: 62.57

GC content: 63.68

Gene sequence:

>603_bases
ATGCGAATCATCGTCGTCGGAGCAACCGGAACTATAGGAAAAGCCGTGGCCAAGCTGTTGTCCACGGAGCATGAAGTGGC
GAAGGTCGCCTTCAGTAGCGGCGACTTGCGGGTGGACATGTCGCGCAAGGATTCCATAGAGTCGATGTTCAAGGAGGTCG
GAGACTTCGATGCCCTGGTCTGCGCGGCGGGAGTCGCCAGGTTCGGCTCCCTGGCTGAATTGACCGACGAGGACTTCCAG
ACCGGGCTCTCCGGGAAGCTGATGGGCCAGGTCAACCTGGTCCGGGTGGGGATGCACTACATCCGGGATAACGGCTCCTT
CACGCTGACCAGCGGGGTCTTGAGCCACCAGCCCATGCCCGGGAGTTCCTCCATCAGCATGATCAACGCGGGGCTCGAAG
GGTTTGTCCGCGCCGCCGCGCTGGAACTGCCGCGCGGGATCAGGATCAACGTGGTGAGCCCCCCCTGGGTGAAGGAGACC
CTTGAGGCGCTGGATATGGACAGCTCGGGGGGAATGCCTGCGCAGGACGTGGCGCAGGCCTACTGGGCCAGCATACACGG
CGCCCGCAGCGGCCTAGTCATCAACGCCCGGGACTTCGCCTGA

Upstream 100 bases:

>100_bases
CAGATATACTTCACGCTGTACCTCGGCCTGTAGGGGGGCGACAGCGCCCCCTGCAGCGGCTCGGGGGCCATAAAAAAGCG
CCAATACGGGAGGTCTCTTT

Downstream 100 bases:

>100_bases
GACAGAAGCAGGAAAGGGCAGGAATTAAAAAGGGGAGCGCCGCTAACAGGCGCTCCCCTTTTCTTGTCTCCGGAAATCCG
GAATCACCTCTCCAGCACTT

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: Short-Chain Dehydrogenase/Reductase SDR; Dehydrogenase; 3-Oxoacyl-[Acyl-Carrier-Protein] Reductase; Short-Chain Dehydrogenase; Oxidoreductase; Oxidoreductase Short Chain Dehydrogenase/Reductase Family; NAD-Dependent Epimerase/Dehydratase; Acyl-Carrier-Protein; Aklaviketone Reductase; 2-Hydroxycyclohexanecarboxyl-CoA Dehydrogenase; Short-Chain Dehydrogenase Protein; Short Chain Dehydrogenase/Reductase Family Oxidoreductase; DTDP-4-Dehydrorhamnose Reductase; Short Chain Dehydrogenase Family Protein; Dehydrogenases With Different Specificities; Short-Chain Dehydrogenase/Reductase

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MRIIVVGATGTIGKAVAKLLSTEHEVAKVAFSSGDLRVDMSRKDSIESMFKEVGDFDALVCAAGVARFGSLAELTDEDFQ
TGLSGKLMGQVNLVRVGMHYIRDNGSFTLTSGVLSHQPMPGSSSISMINAGLEGFVRAAALELPRGIRINVVSPPWVKET
LEALDMDSSGGMPAQDVAQAYWASIHGARSGLVINARDFA

Sequences:

>Translated_200_residues
MRIIVVGATGTIGKAVAKLLSTEHEVAKVAFSSGDLRVDMSRKDSIESMFKEVGDFDALVCAAGVARFGSLAELTDEDFQ
TGLSGKLMGQVNLVRVGMHYIRDNGSFTLTSGVLSHQPMPGSSSISMINAGLEGFVRAAALELPRGIRINVVSPPWVKET
LEALDMDSSGGMPAQDVAQAYWASIHGARSGLVINARDFA
>Mature_200_residues
MRIIVVGATGTIGKAVAKLLSTEHEVAKVAFSSGDLRVDMSRKDSIESMFKEVGDFDALVCAAGVARFGSLAELTDEDFQ
TGLSGKLMGQVNLVRVGMHYIRDNGSFTLTSGVLSHQPMPGSSSISMINAGLEGFVRAAALELPRGIRINVVSPPWVKET
LEALDMDSSGGMPAQDVAQAYWASIHGARSGLVINARDFA

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 21172; Mature: 21172

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIVVGATGTIGKAVAKLLSTEHEVAKVAFSSGDLRVDMSRKDSIESMFKEVGDFDALV
CEEEEEECCCHHHHHHHHHHHCHHHHHHHEECCCCEEEECCHHHHHHHHHHHHCCHHHHH
CAAGVARFGSLAELTDEDFQTGLSGKLMGQVNLVRVGMHYIRDNGSFTLTSGVLSHQPMP
HHHHHHHHCCHHHHCHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCC
GSSSISMINAGLEGFVRAAALELPRGIRINVVSPPWVKETLEALDMDSSGGMPAQDVAQA
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHH
YWASIHGARSGLVINARDFA
HHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure
MRIIVVGATGTIGKAVAKLLSTEHEVAKVAFSSGDLRVDMSRKDSIESMFKEVGDFDALV
CEEEEEECCCHHHHHHHHHHHCHHHHHHHEECCCCEEEECCHHHHHHHHHHHHCCHHHHH
CAAGVARFGSLAELTDEDFQTGLSGKLMGQVNLVRVGMHYIRDNGSFTLTSGVLSHQPMP
HHHHHHHHCCHHHHCHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCC
GSSSISMINAGLEGFVRAAALELPRGIRINVVSPPWVKETLEALDMDSSGGMPAQDVAQA
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHH
YWASIHGARSGLVINARDFA
HHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA