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The map label for this gene is pfk-2 [H]

Identifier: 197118825

GI number: 197118825

Start: 2839689

End: 2840648

Strand: Direct

Name: pfk-2 [H]

Synonym: Gbem_2448

Alternate gene names: 197118825

Gene position: 2839689-2840648 (Clockwise)

Preceding gene: 197118824

Following gene: 197118834

Centisome position: 61.53

GC content: 64.38

Gene sequence:

>960_bases
ATGAAAAAGATAGGGATACTCACCAGCGGCGGGGACTGTTCAGGCATGAACGCGGCCATCAGGGCCGCGACCAGGACCGC
CCTGGGGCACGGGGTACAGGTGCTCGGCTTCCGCAAGGGGTACGCGGGGCTATTGAAGGGGGACTTCCTCGAAATGCAGA
CCAAAGACGTGGCGGGGATCCTGCACCGCGGGGGTACCTTCCTGCAATCGGCCAGAAGCGAGGAATTCCGCACCGTCCTG
GGGAGGGAGAAGGCGGTGCGCCACCTGGAGGAGTTCGGGGTCGAGGGGCTGATCGTCATCGGGGGGGACGGCTCCTTGAA
CGGCGCGCTGGCGCTGCACCGCATGGGGGTGCCGGTAATCGGCATACCGGCCAGCATCGACAACGACATCCCCTTCACCG
ACATGGCGCTCGGCGTGGACACTGCACTCAACAACATCATCTATGCGGTCGACTGCATCAAGGACACGGCGAGCTCGCAC
GACCGGGCCTTCGTCATCGAGGTGATGGGGCGGAATTCCGGGTATCTCGCCAGCATGGCAGCCATCGCCACCGGCGCCGA
GTACGCCATAGTCCCCGAGGTCGAGTGCGACGTAGCCGATCTTTGCAACCAGCTCAGGCACCGCTGGGAGGAAGGGCGCA
GCAACGCCATCATCATCCTGGCCGAAGGGGCCGGGCGCGCCCAGAACATAGCCGACAACATCAAGGACGCCATCGGCTTC
GAGACCCGGGTCACCGTGCTCGGCCACTACCAGAGGGGGGGCGCCCCTTCGGTCTTCGACCGGCTCTTGGGAAGCAGGTT
CGGGCACGCTGCGGTGGAGAACCTCTTGGCCGGGCAGATGGGAAAGATGGTGGGGCTTTGCTGCAACGCGATCGTGCCGA
CCCTTTTGGAGACGGTGGTGGTAAGCGAGAAGAGGCAGCAAGACGAGCTGCACGAGATGTCGCTGATCCTCGGCATCTAG

Upstream 100 bases:

>100_bases
CGCTGTTGTAATCGTTTTCCCCCATGATATATTCAACCAGGCTTTCCTGCGGATCATGAAGATGCTGGGGAGAGCCCATT
AATTCCGGGCGGAGTCACGG

Downstream 100 bases:

>100_bases
CTGCGGTTCGGGAGAGACACAAACGAAAGGGGAGCGCCGGCTGCAAGCGGCGCTCCCCTTTTTTGTTCAGATTCCCCTAG
GCTAGCTAGGGGCGGCGGTA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKKIGILTSGGDCSGMNAAIRAATRTALGHGVQVLGFRKGYAGLLKGDFLEMQTKDVAGILHRGGTFLQSARSEEFRTVL
GREKAVRHLEEFGVEGLIVIGGDGSLNGALALHRMGVPVIGIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSH
DRAFVIEVMGRNSGYLASMAAIATGAEYAIVPEVECDVADLCNQLRHRWEEGRSNAIIILAEGAGRAQNIADNIKDAIGF
ETRVTVLGHYQRGGAPSVFDRLLGSRFGHAAVENLLAGQMGKMVGLCCNAIVPTLLETVVVSEKRQQDELHEMSLILGI

Sequences:

>Translated_319_residues
MKKIGILTSGGDCSGMNAAIRAATRTALGHGVQVLGFRKGYAGLLKGDFLEMQTKDVAGILHRGGTFLQSARSEEFRTVL
GREKAVRHLEEFGVEGLIVIGGDGSLNGALALHRMGVPVIGIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSH
DRAFVIEVMGRNSGYLASMAAIATGAEYAIVPEVECDVADLCNQLRHRWEEGRSNAIIILAEGAGRAQNIADNIKDAIGF
ETRVTVLGHYQRGGAPSVFDRLLGSRFGHAAVENLLAGQMGKMVGLCCNAIVPTLLETVVVSEKRQQDELHEMSLILGI
>Mature_319_residues
MKKIGILTSGGDCSGMNAAIRAATRTALGHGVQVLGFRKGYAGLLKGDFLEMQTKDVAGILHRGGTFLQSARSEEFRTVL
GREKAVRHLEEFGVEGLIVIGGDGSLNGALALHRMGVPVIGIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSH
DRAFVIEVMGRNSGYLASMAAIATGAEYAIVPEVECDVADLCNQLRHRWEEGRSNAIIILAEGAGRAQNIADNIKDAIGF
ETRVTVLGHYQRGGAPSVFDRLLGSRFGHAAVENLLAGQMGKMVGLCCNAIVPTLLETVVVSEKRQQDELHEMSLILGI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=310, Percent_Identity=43.5483870967742, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI266453619, Length=312, Percent_Identity=41.9871794871795, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=41.9871794871795, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=41.9871794871795, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=41.9871794871795, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI48762920, Length=310, Percent_Identity=41.2903225806452, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI1790350, Length=286, Percent_Identity=47.5524475524476, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI25147584, Length=312, Percent_Identity=43.5897435897436, Blast_Score=220, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=40.0641025641026, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=31.8339100346021, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321679, Length=345, Percent_Identity=40.8695652173913, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=36.5217391304348, Blast_Score=189, Evalue=5e-49,
Organism=Drosophila melanogaster, GI28573326, Length=310, Percent_Identity=40.6451612903226, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24652337, Length=310, Percent_Identity=40.3225806451613, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17647809, Length=310, Percent_Identity=40.3225806451613, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 33957; Mature: 33957

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGILTSGGDCSGMNAAIRAATRTALGHGVQVLGFRKGYAGLLKGDFLEMQTKDVAGI
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHCCCHHHHHHHHHHHH
LHRGGTFLQSARSEEFRTVLGREKAVRHLEEFGVEGLIVIGGDGSLNGALALHRMGVPVI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCEE
GIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSHDRAFVIEVMGRNSGYLASMA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
AIATGAEYAIVPEVECDVADLCNQLRHRWEEGRSNAIIILAEGAGRAQNIADNIKDAIGF
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
ETRVTVLGHYQRGGAPSVFDRLLGSRFGHAAVENLLAGQMGKMVGLCCNAIVPTLLETVV
CEEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEKRQQDELHEMSLILGI
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIGILTSGGDCSGMNAAIRAATRTALGHGVQVLGFRKGYAGLLKGDFLEMQTKDVAGI
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHCCCHHHHHHHHHHHH
LHRGGTFLQSARSEEFRTVLGREKAVRHLEEFGVEGLIVIGGDGSLNGALALHRMGVPVI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCEE
GIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSHDRAFVIEVMGRNSGYLASMA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
AIATGAEYAIVPEVECDVADLCNQLRHRWEEGRSNAIIILAEGAGRAQNIADNIKDAIGF
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
ETRVTVLGHYQRGGAPSVFDRLLGSRFGHAAVENLLAGQMGKMVGLCCNAIVPTLLETVV
CEEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEKRQQDELHEMSLILGI
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]