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The map label for this gene is pmbA [H]

Identifier: 197118819

GI number: 197118819

Start: 2831205

End: 2832545

Strand: Reverse

Name: pmbA [H]

Synonym: Gbem_2442

Alternate gene names: 197118819

Gene position: 2832545-2831205 (Counterclockwise)

Preceding gene: 197118820

Following gene: 197118814

Centisome position: 61.37

GC content: 64.65

Gene sequence:

>1341_bases
ATGGAACTAGCCAAGCACGCAGACGCAGTCGAACTGCTCCTCAAGGGGCGCAACCTGGATGGGTACGAGATATTCGTGTC
GCAGTCGCGGGACCTTTCCATCGAGGCCAAACAGGGGCAGGTGGACGCCTTCCGCGCCGCTGAGCCTTTCGGGGTGGCGG
TCCGGTTGAAGGTCGGCGACGGACTCGGTTTCTCCTATTCCACCAGCATGGATCCTGCAGCGCTCGCCATGATGGTCGAC
GGCGCCCTGGTGGCGGCCCGCATGCAGACGCAGGATCCCGGCTACACCCTTGCCGCGCCGGCGATCAGCTACCCTGAACT
ACCCTGGCTTTACGATCCGGAACTTCCCGCCGTGGACGAGGAGCTCAAGGTGGCGCGCGTGTTGGAATTGGAGCGGCTGG
TGCTGGCCACGGACCCGCGGGTAAAAAGGGTGAGAAAGTGCAGCTACGGCGAATCGGTCTACTCCAGCTTCATCAGGAAT
TCGCTGGGTCTTGAGAAGGGGTACCGCGGCAGCTACGTGACCTGCTCCGCCTCGGCCGTGGCAGAGGAGGGGGGCGACGC
GCAAAGCGGCTGGGATTTCGCCTTCTCCCCCTCTTTCTCCGGCATCGACATCGAGGCGGTGGCCAAAGGGGCGGGCAGCA
AGGCGACGGCCTTACTGGGGGCGCGCAGCATCCCCGGCATGCGCTGTCCGGTCGTCCTCGACAACCACGTCGCCGCAGGC
ATCGTCGAGCTCTTGGCCCCCTCCTTCCTCGGGGAGGCGGTCCACAAGGGGAAATCGCTCTTCATGGGCAAAGAAGGTGA
GCAGGTATTCTCCGAACTGGTCACGCTGCACGACGACGGCCTTCTGCTCGACGGGACGGGAACCGCCCCCTGTGACGGCG
AGGGTGTGCCGCAGCGGGATACGCCGCTTGTGACCGCCGGGGTGCTGCAGGGGTTTTTGTACGACAGCTACTGGGGGAAA
AAGGCTGGGAAATCCTCTACCGGCAACGCCCAGCGCGGAGGCATTAAAGGGGCGCCCCGCGGCGGCGCCAACAACCTGAT
GATAGATCCTGGCCAGGCGAGCCTGGAAACGCTTCTGGCCGGTGTCGAGCGCGGGGTGCTGATCACCGAGGTGATGGGGA
TGCACACGGCCAACACCATCTCGGGGGACTTTTCCGTGGGGGCCTCGGGTTTCTATCTGGAGCGCGGGGAGATTCTCTAT
CCGGTGAAGGGTATGGCACTGGCGGGGAACCTGATGCAGCTGTTCAAGGGAGTGGACCAGGTGGGAAGCGACCTCCGCTT
CTTCGGCGGTGCGGGGGCGCCGTCGCTTAGGATCGCGGAGCTGGAGATCAGCGGCAGCTAA

Upstream 100 bases:

>100_bases
ATGGTGCTGGATCTTTAAAGCAGAAGCGGGGCTAGGGGCTAAAAGGCCCCCAACCCCCAGCCCCTAGCCCCTGCCTTTGC
CCCGCCTTCAGAGGTTAAAC

Downstream 100 bases:

>100_bases
GACGCATGAAAAGGCCGCACCTGTAAAGGGTGCGGCCTTTTTTCATTTCTTCAACCGCCGGGAAGCTTAGGGCTCTTGGT
GCAGTATCTCGATCAACCTG

Product: zinc protease PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MELAKHADAVELLLKGRNLDGYEIFVSQSRDLSIEAKQGQVDAFRAAEPFGVAVRLKVGDGLGFSYSTSMDPAALAMMVD
GALVAARMQTQDPGYTLAAPAISYPELPWLYDPELPAVDEELKVARVLELERLVLATDPRVKRVRKCSYGESVYSSFIRN
SLGLEKGYRGSYVTCSASAVAEEGGDAQSGWDFAFSPSFSGIDIEAVAKGAGSKATALLGARSIPGMRCPVVLDNHVAAG
IVELLAPSFLGEAVHKGKSLFMGKEGEQVFSELVTLHDDGLLLDGTGTAPCDGEGVPQRDTPLVTAGVLQGFLYDSYWGK
KAGKSSTGNAQRGGIKGAPRGGANNLMIDPGQASLETLLAGVERGVLITEVMGMHTANTISGDFSVGASGFYLERGEILY
PVKGMALAGNLMQLFKGVDQVGSDLRFFGGAGAPSLRIAELEISGS

Sequences:

>Translated_446_residues
MELAKHADAVELLLKGRNLDGYEIFVSQSRDLSIEAKQGQVDAFRAAEPFGVAVRLKVGDGLGFSYSTSMDPAALAMMVD
GALVAARMQTQDPGYTLAAPAISYPELPWLYDPELPAVDEELKVARVLELERLVLATDPRVKRVRKCSYGESVYSSFIRN
SLGLEKGYRGSYVTCSASAVAEEGGDAQSGWDFAFSPSFSGIDIEAVAKGAGSKATALLGARSIPGMRCPVVLDNHVAAG
IVELLAPSFLGEAVHKGKSLFMGKEGEQVFSELVTLHDDGLLLDGTGTAPCDGEGVPQRDTPLVTAGVLQGFLYDSYWGK
KAGKSSTGNAQRGGIKGAPRGGANNLMIDPGQASLETLLAGVERGVLITEVMGMHTANTISGDFSVGASGFYLERGEILY
PVKGMALAGNLMQLFKGVDQVGSDLRFFGGAGAPSLRIAELEISGS
>Mature_446_residues
MELAKHADAVELLLKGRNLDGYEIFVSQSRDLSIEAKQGQVDAFRAAEPFGVAVRLKVGDGLGFSYSTSMDPAALAMMVD
GALVAARMQTQDPGYTLAAPAISYPELPWLYDPELPAVDEELKVARVLELERLVLATDPRVKRVRKCSYGESVYSSFIRN
SLGLEKGYRGSYVTCSASAVAEEGGDAQSGWDFAFSPSFSGIDIEAVAKGAGSKATALLGARSIPGMRCPVVLDNHVAAG
IVELLAPSFLGEAVHKGKSLFMGKEGEQVFSELVTLHDDGLLLDGTGTAPCDGEGVPQRDTPLVTAGVLQGFLYDSYWGK
KAGKSSTGNAQRGGIKGAPRGGANNLMIDPGQASLETLLAGVERGVLITEVMGMHTANTISGDFSVGASGFYLERGEILY
PVKGMALAGNLMQLFKGVDQVGSDLRFFGGAGAPSLRIAELEISGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=432, Percent_Identity=28.7037037037037, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789640, Length=474, Percent_Identity=26.3713080168776, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46914; Mature: 46914

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELAKHADAVELLLKGRNLDGYEIFVSQSRDLSIEAKQGQVDAFRAAEPFGVAVRLKVGD
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHCCCCCCEEEEEEECC
GLGFSYSTSMDPAALAMMVDGALVAARMQTQDPGYTLAAPAISYPELPWLYDPELPAVDE
CCCCCCCCCCCHHHHHHHHCCHHEEEEHHCCCCCEEEECCCCCCCCCCCEECCCCCCCCH
ELKVARVLELERLVLATDPRVKRVRKCSYGESVYSSFIRNSLGLEKGYRGSYVTCSASAV
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
AEEGGDAQSGWDFAFSPSFSGIDIEAVAKGAGSKATALLGARSIPGMRCPVVLDNHVAAG
HHCCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCEEECCHHHHH
IVELLAPSFLGEAVHKGKSLFMGKEGEQVFSELVTLHDDGLLLDGTGTAPCDGEGVPQRD
HHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
TPLVTAGVLQGFLYDSYWGKKAGKSSTGNAQRGGIKGAPRGGANNLMIDPGQASLETLLA
CCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHH
GVERGVLITEVMGMHTANTISGDFSVGASGFYLERGEILYPVKGMALAGNLMQLFKGVDQ
HHHCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHH
VGSDLRFFGGAGAPSLRIAELEISGS
HCCCEEEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MELAKHADAVELLLKGRNLDGYEIFVSQSRDLSIEAKQGQVDAFRAAEPFGVAVRLKVGD
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHCCCCCCEEEEEEECC
GLGFSYSTSMDPAALAMMVDGALVAARMQTQDPGYTLAAPAISYPELPWLYDPELPAVDE
CCCCCCCCCCCHHHHHHHHCCHHEEEEHHCCCCCEEEECCCCCCCCCCCEECCCCCCCCH
ELKVARVLELERLVLATDPRVKRVRKCSYGESVYSSFIRNSLGLEKGYRGSYVTCSASAV
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
AEEGGDAQSGWDFAFSPSFSGIDIEAVAKGAGSKATALLGARSIPGMRCPVVLDNHVAAG
HHCCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCEEECCHHHHH
IVELLAPSFLGEAVHKGKSLFMGKEGEQVFSELVTLHDDGLLLDGTGTAPCDGEGVPQRD
HHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
TPLVTAGVLQGFLYDSYWGKKAGKSSTGNAQRGGIKGAPRGGANNLMIDPGQASLETLLA
CCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHH
GVERGVLITEVMGMHTANTISGDFSVGASGFYLERGEILYPVKGMALAGNLMQLFKGVDQ
HHHCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHH
VGSDLRFFGGAGAPSLRIAELEISGS
HCCCEEEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]