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The map label for this gene is queA

Identifier: 197118813

GI number: 197118813

Start: 2824182

End: 2825207

Strand: Reverse

Name: queA

Synonym: Gbem_2436

Alternate gene names: 197118813

Gene position: 2825207-2824182 (Counterclockwise)

Preceding gene: 197118814

Following gene: 197118812

Centisome position: 61.22

GC content: 63.74

Gene sequence:

>1026_bases
ATGTTTCTTTCCGATTTCGATTACGAACTTCCACCGGAGCTGATAGCGCAGCACCCGGCGAGCCGCAGGGACGCCTCCAG
GCTTTTGACCCTTGACCGCTTAAGCGGCGCCATCGGCGAGGCAACCGTGGCCTCTATCGCCGCGCAGTTCCTCCCGGGCG
ATCTCCTGGTCTTGAACGACACCCGGGTCATCCCGGCGAGGCTGCACGGTCATAAGGAATCGGGGGGGGCTGTAGAGATC
TTCCTGGTGCGCCGGGTCGCAGGGGAACCGGAGTGCTGGAGTTGCCTGATCAAGGCCTCCAAGAGTCCCAAAAACGGTTG
CCGAGTGGTGTTTCCCGGCGGGGTGACCGCCACGGTGCTAGAGCGCGACGGCGGCGACTGGCGGGTCCTCTTCGAGGGGA
GCGACGACTTCATGGCATGGCTGGAGCAAACCGGCAGCATGCCGCTTCCACCCTACATCAAGCGGGCCCCCGAAGGTGAG
GACCTGGAGCGTTACCAGACAGTGTTCGCGCGCGAGAAGGGTGCCGTAGCCGCCCCGACAGCCGGGCTGCACTTCACCCC
GGCGATCCTGGACGAGATCAGGGGGCGCGGCGTCGAGATCGTCTCCGTCACCCTGCATGTGGGGCTTGGGACCTTCATGC
CGATCCGGGTGGAGGACCTCTCCGAGCACACCATGCACCGGGAGCTTTACCGGATCCCGCAGGAAACCGCCGCCGCGATC
CGCCGCACCAAGGAGCGGGGGGGTAGGGTGGTGGCCCTGGGAACCACCTCGCTCAGGGCGCTGGAGCATGCGGCGGCCTC
AGGGGAAGTAGCAGCCGGCGAGCGCGAAGCGGATATTTTTATCCTTCCCGGTTACCGGTTTAGGGTTGTCGACGCGTTGA
TAACAAATTTTCATCTCCCCAAATCGACTCTCTTTATGCTAGTGTGCGCATTCGCCGGGAAGGAGGCGATGCTGGAAGCC
TACCGCGAGGCGGTTTCCCGGCGCTTCAGGTTCTTCAGCTACGGCGACGCCATGTTCATCGGTTAA

Upstream 100 bases:

>100_bases
TGGGGCGCCAAGGGGCGCGCCATCGAGGGGTTCGATTACCGCGAGATACTCAGTTACTACTATCCGGGCGTGAAGCTTGC
GGGCGGGTACGGTCGATAGC

Downstream 100 bases:

>100_bases
CGGAACTCTTCCCCACAGAGGGAAGCACTCCAACCCTTGCCCCCTCGCGCCGGGGGAGGGGGGAGTAAAACGAATACAAG
CAGCAAACGGGATATTTTTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MFLSDFDYELPPELIAQHPASRRDASRLLTLDRLSGAIGEATVASIAAQFLPGDLLVLNDTRVIPARLHGHKESGGAVEI
FLVRRVAGEPECWSCLIKASKSPKNGCRVVFPGGVTATVLERDGGDWRVLFEGSDDFMAWLEQTGSMPLPPYIKRAPEGE
DLERYQTVFAREKGAVAAPTAGLHFTPAILDEIRGRGVEIVSVTLHVGLGTFMPIRVEDLSEHTMHRELYRIPQETAAAI
RRTKERGGRVVALGTTSLRALEHAAASGEVAAGEREADIFILPGYRFRVVDALITNFHLPKSTLFMLVCAFAGKEAMLEA
YREAVSRRFRFFSYGDAMFIG

Sequences:

>Translated_341_residues
MFLSDFDYELPPELIAQHPASRRDASRLLTLDRLSGAIGEATVASIAAQFLPGDLLVLNDTRVIPARLHGHKESGGAVEI
FLVRRVAGEPECWSCLIKASKSPKNGCRVVFPGGVTATVLERDGGDWRVLFEGSDDFMAWLEQTGSMPLPPYIKRAPEGE
DLERYQTVFAREKGAVAAPTAGLHFTPAILDEIRGRGVEIVSVTLHVGLGTFMPIRVEDLSEHTMHRELYRIPQETAAAI
RRTKERGGRVVALGTTSLRALEHAAASGEVAAGEREADIFILPGYRFRVVDALITNFHLPKSTLFMLVCAFAGKEAMLEA
YREAVSRRFRFFSYGDAMFIG
>Mature_341_residues
MFLSDFDYELPPELIAQHPASRRDASRLLTLDRLSGAIGEATVASIAAQFLPGDLLVLNDTRVIPARLHGHKESGGAVEI
FLVRRVAGEPECWSCLIKASKSPKNGCRVVFPGGVTATVLERDGGDWRVLFEGSDDFMAWLEQTGSMPLPPYIKRAPEGE
DLERYQTVFAREKGAVAAPTAGLHFTPAILDEIRGRGVEIVSVTLHVGLGTFMPIRVEDLSEHTMHRELYRIPQETAAAI
RRTKERGGRVVALGTTSLRALEHAAASGEVAAGEREADIFILPGYRFRVVDALITNFHLPKSTLFMLVCAFAGKEAMLEA
YREAVSRRFRFFSYGDAMFIG

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=48.1159420289855, Blast_Score=320, Evalue=6e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_GEOBB (B5EFX9)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139240.1
- ProteinModelPortal:   B5EFX9
- SMR:   B5EFX9
- GeneID:   6782433
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2436
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37516; Mature: 37516

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSDFDYELPPELIAQHPASRRDASRLLTLDRLSGAIGEATVASIAAQFLPGDLLVLND
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEC
TRVIPARLHGHKESGGAVEIFLVRRVAGEPECWSCLIKASKSPKNGCRVVFPGGVTATVL
CEEEEHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEE
ERDGGDWRVLFEGSDDFMAWLEQTGSMPLPPYIKRAPEGEDLERYQTVFAREKGAVAAPT
ECCCCCEEEEEECCHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEECCC
AGLHFTPAILDEIRGRGVEIVSVTLHVGLGTFMPIRVEDLSEHTMHRELYRIPQETAAAI
CCCEECHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHH
RRTKERGGRVVALGTTSLRALEHAAASGEVAAGEREADIFILPGYRFRVVDALITNFHLP
HHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCC
KSTLFMLVCAFAGKEAMLEAYREAVSRRFRFFSYGDAMFIG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCEEEEC
>Mature Secondary Structure
MFLSDFDYELPPELIAQHPASRRDASRLLTLDRLSGAIGEATVASIAAQFLPGDLLVLND
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEC
TRVIPARLHGHKESGGAVEIFLVRRVAGEPECWSCLIKASKSPKNGCRVVFPGGVTATVL
CEEEEHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEE
ERDGGDWRVLFEGSDDFMAWLEQTGSMPLPPYIKRAPEGEDLERYQTVFAREKGAVAAPT
ECCCCCEEEEEECCHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEECCC
AGLHFTPAILDEIRGRGVEIVSVTLHVGLGTFMPIRVEDLSEHTMHRELYRIPQETAAAI
CCCEECHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHH
RRTKERGGRVVALGTTSLRALEHAAASGEVAAGEREADIFILPGYRFRVVDALITNFHLP
HHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCC
KSTLFMLVCAFAGKEAMLEAYREAVSRRFRFFSYGDAMFIG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA