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The map label for this gene is idnO [C]

Identifier: 197118742

GI number: 197118742

Start: 2738912

End: 2739643

Strand: Direct

Name: idnO [C]

Synonym: Gbem_2361

Alternate gene names: 197118742

Gene position: 2738912-2739643 (Clockwise)

Preceding gene: 197118741

Following gene: 197118754

Centisome position: 59.35

GC content: 61.2

Gene sequence:

>732_bases
ATGGAAATCGCAGGAAAAACAGTTTTGGTGACCGGCGCGAACGGCGGGATCGGTCGTGCTCTGGTCGAGGGACTGCTCAA
AAAAGGGGCCGCCAAGGTTTACGCAGCGGCAAGGACCATCGAAGCCGTGGCGGAACTTTCCAAAATCGACAAGCGAGTGG
CGGCGGTAAGGCTTGAGGTCACCCATGCCACCAGCGTGGCTGCAGCAGCGGAGCACTGCCCGGAGGTCGACCTCGTCATC
AACAACGCAGGGATCAACCGCTGCGCGGGTTTTATGGCTGAAAACGCCATGGAGAACGCGCGCCAGGAAATCAAGGTCAA
CTACCTGGGGACGCTGGCCATGTGCCGCGCCTTCGCCCCGGCCCTCATCGAGCGCAAAGGATGCATCGTCAACGTTTGTT
CCGTCATCGGGCTGGTCAACCTCCCGGTTAACGGCACCTACAGCGCCTCGAAGGCTGCGGGACACTCTCTGTTGCAGGGG
ATCCGGGCCGAATTGGCGCCGCACGGCGTGAAGGTGCTGGGGATCTATCCCGGCCCGGTCGACACCAGGATGACCTCAGG
CCAGGAGATGCCCAAGGCGACTACCGAGGAAGTGGTGCAAGCGATACTGGAAGGGATCGAAAAAGAGCAGGAGGAGATTT
ATCCCGACGCGATGTCGAAAGAGGTTTCCGCAGCGCTTCTGCAGGCGCCCAAAGAGGTCGAGCGGCAGTTCTCCGCCATG
ATCCCCGCCTAG

Upstream 100 bases:

>100_bases
GCCGTGATGGTGCCGGCTTCCTGACATAACAAAGATGAACTTCGAACCTGGGGGCGGACAGCGAGCCGCGTGAAGGTAGA
CAACGAAAAAGGAGCAAGAA

Downstream 100 bases:

>100_bases
AGGAAATGGTCAGACGGGGGTCCGCTAAAGTGCTACGGCGACCAGCGCGACCACCCGCCTCCTTCCCTGCGAGTCCCCCA
TCACCTGGAGCGGGAGTTCG

Product: short chain dehydrogenase

Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MEIAGKTVLVTGANGGIGRALVEGLLKKGAAKVYAAARTIEAVAELSKIDKRVAAVRLEVTHATSVAAAAEHCPEVDLVI
NNAGINRCAGFMAENAMENARQEIKVNYLGTLAMCRAFAPALIERKGCIVNVCSVIGLVNLPVNGTYSASKAAGHSLLQG
IRAELAPHGVKVLGIYPGPVDTRMTSGQEMPKATTEEVVQAILEGIEKEQEEIYPDAMSKEVSAALLQAPKEVERQFSAM
IPA

Sequences:

>Translated_243_residues
MEIAGKTVLVTGANGGIGRALVEGLLKKGAAKVYAAARTIEAVAELSKIDKRVAAVRLEVTHATSVAAAAEHCPEVDLVI
NNAGINRCAGFMAENAMENARQEIKVNYLGTLAMCRAFAPALIERKGCIVNVCSVIGLVNLPVNGTYSASKAAGHSLLQG
IRAELAPHGVKVLGIYPGPVDTRMTSGQEMPKATTEEVVQAILEGIEKEQEEIYPDAMSKEVSAALLQAPKEVERQFSAM
IPA
>Mature_243_residues
MEIAGKTVLVTGANGGIGRALVEGLLKKGAAKVYAAARTIEAVAELSKIDKRVAAVRLEVTHATSVAAAAEHCPEVDLVI
NNAGINRCAGFMAENAMENARQEIKVNYLGTLAMCRAFAPALIERKGCIVNVCSVIGLVNLPVNGTYSASKAAGHSLLQG
IRAELAPHGVKVLGIYPGPVDTRMTSGQEMPKATTEEVVQAILEGIEKEQEEIYPDAMSKEVSAALLQAPKEVERQFSAM
IPA

Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7706318, Length=245, Percent_Identity=29.3877551020408, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI20149619, Length=235, Percent_Identity=26.3829787234043, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI150247226, Length=202, Percent_Identity=31.6831683168317, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI223718074, Length=250, Percent_Identity=31.2, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI19743808, Length=233, Percent_Identity=29.6137339055794, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI32455239, Length=231, Percent_Identity=30.3030303030303, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI5031765, Length=231, Percent_Identity=30.3030303030303, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI210032112, Length=219, Percent_Identity=28.310502283105, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI210032110, Length=231, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI44680136, Length=229, Percent_Identity=27.9475982532751, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI44680133, Length=229, Percent_Identity=27.9475982532751, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI17738292, Length=229, Percent_Identity=27.9475982532751, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI4504503, Length=226, Percent_Identity=29.646017699115, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI40254992, Length=186, Percent_Identity=30.6451612903226, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI22507405, Length=219, Percent_Identity=27.3972602739726, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI7657478, Length=248, Percent_Identity=27.4193548387097, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790717, Length=193, Percent_Identity=34.7150259067358, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082100, Length=215, Percent_Identity=33.0232558139535, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786701, Length=194, Percent_Identity=31.4432989690722, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787335, Length=195, Percent_Identity=30.2564102564103, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787820, Length=180, Percent_Identity=29.4444444444444, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082160, Length=212, Percent_Identity=27.8301886792453, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71997402, Length=173, Percent_Identity=33.5260115606936, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI115534660, Length=226, Percent_Identity=30.5309734513274, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=209, Percent_Identity=33.9712918660287, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557780, Length=243, Percent_Identity=27.9835390946502, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17570069, Length=191, Percent_Identity=30.8900523560209, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=27.8884462151394, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI72001658, Length=232, Percent_Identity=29.7413793103448, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=210, Percent_Identity=27.6190476190476, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=211, Percent_Identity=26.5402843601896, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=217, Percent_Identity=30.8755760368664, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=195, Percent_Identity=29.2307692307692, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17542178, Length=237, Percent_Identity=29.1139240506329, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322067, Length=247, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323882, Length=242, Percent_Identity=28.099173553719, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24639444, Length=223, Percent_Identity=35.4260089686099, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21358495, Length=253, Percent_Identity=28.8537549407115, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24643142, Length=184, Percent_Identity=32.0652173913043, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24651139, Length=226, Percent_Identity=26.1061946902655, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI23397609, Length=191, Percent_Identity=29.3193717277487, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24649181, Length=261, Percent_Identity=26.4367816091954, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24580925, Length=195, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357041, Length=191, Percent_Identity=30.8900523560209, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24644339, Length=209, Percent_Identity=28.2296650717703, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.69 [C]

Molecular weight: Translated: 25577; Mature: 25577

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIAGKTVLVTGANGGIGRALVEGLLKKGAAKVYAAARTIEAVAELSKIDKRVAAVRLEV
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEH
THATSVAAAAEHCPEVDLVINNAGINRCAGFMAENAMENARQEIKVNYLGTLAMCRAFAP
HHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIERKGCIVNVCSVIGLVNLPVNGTYSASKAAGHSLLQGIRAELAPHGVKVLGIYPGPV
HHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
DTRMTSGQEMPKATTEEVVQAILEGIEKEQEEIYPDAMSKEVSAALLQAPKEVERQFSAM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHC
IPA
CCC
>Mature Secondary Structure
MEIAGKTVLVTGANGGIGRALVEGLLKKGAAKVYAAARTIEAVAELSKIDKRVAAVRLEV
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEH
THATSVAAAAEHCPEVDLVINNAGINRCAGFMAENAMENARQEIKVNYLGTLAMCRAFAP
HHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIERKGCIVNVCSVIGLVNLPVNGTYSASKAAGHSLLQGIRAELAPHGVKVLGIYPGPV
HHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
DTRMTSGQEMPKATTEEVVQAILEGIEKEQEEIYPDAMSKEVSAALLQAPKEVERQFSAM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHC
IPA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH; NADP+; NADPH [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} 0.02 {NADP+}} [C]

Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA