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The map label for this gene is pgk

Identifier: 197118718

GI number: 197118718

Start: 2713161

End: 2714360

Strand: Direct

Name: pgk

Synonym: Gbem_2337

Alternate gene names: 197118718

Gene position: 2713161-2714360 (Clockwise)

Preceding gene: 197118717

Following gene: 197118719

Centisome position: 58.79

GC content: 60.33

Gene sequence:

>1200_bases
ATGTCAATCCGCTATATCGATGAGATCGAAAACCTGTCCGGCAAGAAGCTCTTCATGCGCGTGGACTTCAACGTGCCGCT
GGACGACCACCAGAACATCACCGAGGACACACGGATTCGCGCCGTCCTCCCCTCCATCAATTATGCCCTCGACCAAAAGG
CCAAGATCATCCTCGCTTCGCACCTTGGGCGCCCCAAGGGTGAGCGCAAGGAAAAATACTCCATGGCTCCCGCAGCCAAG
CGCCTCTCCAGGCTTCTGGGCAAGGAAGTGAAACTGGCGTCCGACTGCATCGGGGACGAGGTGAAGGCTATGATCAACGC
CATGCAGCCCGGCGACGTGATCATGCTGGAGAACGTCCGTTTCTACGCCGGCGAAGAGAAAAACGACGCCGACTTCGCCA
AGGCCCTCGCCAACGACTGCGACGTCTATGTCAACGACGCCTTCGCAGTTTCCCACCGGGCCCACGCCTCGGTCGAGGCC
ATCACCAACTGCTTCCCCGTGGTGGCTGCCGGCTTCCTGATGAAAAACGAGATGAATTACTTCGAAAAGGCCATGAAGAA
CCCGATCCGCCCCCTGGTCGCCATCCTGGGCGGCGCCAAGGTCTCCGGCAAGATCGAGGTGCTTGAGAACCTCTGCGACA
AGGTCGACAAGATCATCATCGGCGGCGGCATGGCCTTCACCTTCCTCAAGGGGATGGGCTACAACGTGGGCAAATCGCTG
GTAGAGGACAACCTGATAGATACGGCGATGAAGACCTACGAGAAGGCGCGCGCCCAGGGGGTCAAGTTCTACCTTCCCGT
AGACTGCGTCGTGGCCGATCAGTTCAATCCGGCAGCCGAGACCAAGGTCTGCCCGATCCAGGAGATCCCTGAAGGGTGGA
TGGCGCTCGACGTCGGCCCCGCCACCGTCACCCTCTTCACCGAGGCGCTGCAAAACGCGAAGACCATCGTCTGGAACGGG
CCGATGGGGGTGTTCGAGATGGATGCCTTCTCCCGCGGCACCTTCGCCATGGTCTCCGCCGTAGCCAACTCCTACGCGCT
TACCATCGTCGGCGGCGGTGACACCGACGTCGCTGTGCATCGCGCTGGCGAGTACGCAAAGATCAGCTACATCTCCACCG
GCGGCGGCGCGTTCCTCGAACTACTCGAAGGGAAGAAGCTCCCCGGCATAAAGGTCCTGGAAGACAAGGGGCACCAATAA

Upstream 100 bases:

>100_bases
TCCTTTAGGAAAAGGTGGTACTTGGAAGGGTAGATGTGATAAGTAGACGGGGGGGAGGCTGCTCCTCCCCGTATTTATTT
TTATTGATTCAGGAGGTGTC

Downstream 100 bases:

>100_bases
AAACAGATAAAGATAAAGATAAAGATGGAGATTAAGACTAAGATTAAGATTAAGAATTAAGGCTAAGGTCGTGAGAATGG
TTTTTGGTTTTCCTCAATCT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSIRYIDEIENLSGKKLFMRVDFNVPLDDHQNITEDTRIRAVLPSINYALDQKAKIILASHLGRPKGERKEKYSMAPAAK
RLSRLLGKEVKLASDCIGDEVKAMINAMQPGDVIMLENVRFYAGEEKNDADFAKALANDCDVYVNDAFAVSHRAHASVEA
ITNCFPVVAAGFLMKNEMNYFEKAMKNPIRPLVAILGGAKVSGKIEVLENLCDKVDKIIIGGGMAFTFLKGMGYNVGKSL
VEDNLIDTAMKTYEKARAQGVKFYLPVDCVVADQFNPAAETKVCPIQEIPEGWMALDVGPATVTLFTEALQNAKTIVWNG
PMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDVAVHRAGEYAKISYISTGGGAFLELLEGKKLPGIKVLEDKGHQ

Sequences:

>Translated_399_residues
MSIRYIDEIENLSGKKLFMRVDFNVPLDDHQNITEDTRIRAVLPSINYALDQKAKIILASHLGRPKGERKEKYSMAPAAK
RLSRLLGKEVKLASDCIGDEVKAMINAMQPGDVIMLENVRFYAGEEKNDADFAKALANDCDVYVNDAFAVSHRAHASVEA
ITNCFPVVAAGFLMKNEMNYFEKAMKNPIRPLVAILGGAKVSGKIEVLENLCDKVDKIIIGGGMAFTFLKGMGYNVGKSL
VEDNLIDTAMKTYEKARAQGVKFYLPVDCVVADQFNPAAETKVCPIQEIPEGWMALDVGPATVTLFTEALQNAKTIVWNG
PMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDVAVHRAGEYAKISYISTGGGAFLELLEGKKLPGIKVLEDKGHQ
>Mature_398_residues
SIRYIDEIENLSGKKLFMRVDFNVPLDDHQNITEDTRIRAVLPSINYALDQKAKIILASHLGRPKGERKEKYSMAPAAKR
LSRLLGKEVKLASDCIGDEVKAMINAMQPGDVIMLENVRFYAGEEKNDADFAKALANDCDVYVNDAFAVSHRAHASVEAI
TNCFPVVAAGFLMKNEMNYFEKAMKNPIRPLVAILGGAKVSGKIEVLENLCDKVDKIIIGGGMAFTFLKGMGYNVGKSLV
EDNLIDTAMKTYEKARAQGVKFYLPVDCVVADQFNPAAETKVCPIQEIPEGWMALDVGPATVTLFTEALQNAKTIVWNGP
MGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDVAVHRAGEYAKISYISTGGGAFLELLEGKKLPGIKVLEDKGHQ

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=407, Percent_Identity=46.4373464373464, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI31543397, Length=407, Percent_Identity=44.7174447174447, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1789294, Length=390, Percent_Identity=43.5897435897436, Blast_Score=311, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI17508823, Length=409, Percent_Identity=47.1882640586797, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319857, Length=406, Percent_Identity=49.2610837438424, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17136394, Length=414, Percent_Identity=46.1352657004831, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=44.3645083932854, Blast_Score=328, Evalue=4e-90,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_GEOBB (B5EF40)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139145.1
- ProteinModelPortal:   B5EF40
- SMR:   B5EF40
- GeneID:   6782333
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2337
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43490; Mature: 43359

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 120-120 BINDING 153-153 BINDING 204-204 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRYIDEIENLSGKKLFMRVDFNVPLDDHQNITEDTRIRAVLPSINYALDQKAKIILAS
CCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHCCCHHEEEEH
HLGRPKGERKEKYSMAPAAKRLSRLLGKEVKLASDCIGDEVKAMINAMQPGDVIMLENVR
HCCCCCCCHHHHHCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECEE
FYAGEEKNDADFAKALANDCDVYVNDAFAVSHRAHASVEAITNCFPVVAAGFLMKNEMNY
EECCCCCCHHHHHHHHHCCCCEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKAMKNPIRPLVAILGGAKVSGKIEVLENLCDKVDKIIIGGGMAFTFLKGMGYNVGKSL
HHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHH
VEDNLIDTAMKTYEKARAQGVKFYLPVDCVVADQFNPAAETKVCPIQEIPEGWMALDVGP
HHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCHHHCCCCCEEEECCC
ATVTLFTEALQNAKTIVWNGPMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDVAVH
HHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCEEEE
RAGEYAKISYISTGGGAFLELLEGKKLPGIKVLEDKGHQ
CCCCEEEEEEEECCCHHHHHHHCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
SIRYIDEIENLSGKKLFMRVDFNVPLDDHQNITEDTRIRAVLPSINYALDQKAKIILAS
CCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHCCCHHEEEEH
HLGRPKGERKEKYSMAPAAKRLSRLLGKEVKLASDCIGDEVKAMINAMQPGDVIMLENVR
HCCCCCCCHHHHHCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECEE
FYAGEEKNDADFAKALANDCDVYVNDAFAVSHRAHASVEAITNCFPVVAAGFLMKNEMNY
EECCCCCCHHHHHHHHHCCCCEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKAMKNPIRPLVAILGGAKVSGKIEVLENLCDKVDKIIIGGGMAFTFLKGMGYNVGKSL
HHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHH
VEDNLIDTAMKTYEKARAQGVKFYLPVDCVVADQFNPAAETKVCPIQEIPEGWMALDVGP
HHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCHHHCCCCCEEEECCC
ATVTLFTEALQNAKTIVWNGPMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDVAVH
HHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCEEEE
RAGEYAKISYISTGGGAFLELLEGKKLPGIKVLEDKGHQ
CCCCEEEEEEEECCCHHHHHHHCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA