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The map label for this gene is tpi

Identifier: 197118719

GI number: 197118719

Start: 2714554

End: 2715309

Strand: Direct

Name: tpi

Synonym: Gbem_2338

Alternate gene names: 197118719

Gene position: 2714554-2715309 (Clockwise)

Preceding gene: 197118718

Following gene: 197118720

Centisome position: 58.82

GC content: 61.38

Gene sequence:

>756_bases
ATGCGCAAGCCGGTCATAGCAGGCAACTGGAAACTGTTCAAGACTAAGAACGAGGCGCTCGCACTGATAGAAGAATTGGC
ACCGCTGGTTTCCGGCGTGGACAGCGTGGAGATCGTGGTGGCGCCGGTGTTCACCGTGCTCCCCACCCTCCCCGCTGCGC
TCGCCGGCACCGGCATAAGCCTTGCCGCCCAGGACGTATTCTGGGAAGAAGAAGGCGCCTTTACCGGCGAAGTTTCGCCG
CGCATGCTTCTCGATGCCGGTGCAAGCCACGTCATCATCGGCCATTCCGAACGCCGGCAGTATTTCGGCGAGACCGAGGA
AACGGTCAACAAGAAGGTGAAGGCCGCACTCAAAGGGGCGCTTGTCCCCATCGTGTGCATCGGCGAAACCCTGGAAGCGC
GCGAAGCTGGCGATACCTTCAAGGTGCTGGAGCGCCAGCTGAAGGGTGGGCTCGAGGGGCTGACAGGGACACAGTTCGCG
CCGGTCATCGTAGCCTACGAGCCGGTCTGGGCCATCGGCACCGGCAAGGTCGCCTCCGACGACCAGGCGCAGGAGGCACA
TGCCTTCATTCGCGGGGTCATCGCAGGACTCTTCGGCAAGTCGGCGGCGGATAAAGTGAGGATTCTCTACGGCGGCTCCG
TGAAGCCGGATAACGTGAAAGGACTCATGTCGCGCCCTGACATAGACGGTGCGCTCGTGGGAGGCGCAAGTCTCAAAGGC
GCATCTTTTGCCTCAATCGTGCGTTACAGTGAATAA

Upstream 100 bases:

>100_bases
TCAATCTTAATCTTTATCTCAATCTGTATTTAGGTTTTTCCGTCTTTCCTTAATCTTTATCTTTATCTTTATCTCAATGT
ATTTCTCCTGGAGGTATTAG

Downstream 100 bases:

>100_bases
ATTCTTGGTTATTTACATCAAATATGCTAGAAAGTCTCTTTGAACACTCTCGGAGGTAAGGCTTTAAATGACAATTCTAC
TGGTAACCCTGCATATCGTG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRKPVIAGNWKLFKTKNEALALIEELAPLVSGVDSVEIVVAPVFTVLPTLPAALAGTGISLAAQDVFWEEEGAFTGEVSP
RMLLDAGASHVIIGHSERRQYFGETEETVNKKVKAALKGALVPIVCIGETLEAREAGDTFKVLERQLKGGLEGLTGTQFA
PVIVAYEPVWAIGTGKVASDDQAQEAHAFIRGVIAGLFGKSAADKVRILYGGSVKPDNVKGLMSRPDIDGALVGGASLKG
ASFASIVRYSE

Sequences:

>Translated_251_residues
MRKPVIAGNWKLFKTKNEALALIEELAPLVSGVDSVEIVVAPVFTVLPTLPAALAGTGISLAAQDVFWEEEGAFTGEVSP
RMLLDAGASHVIIGHSERRQYFGETEETVNKKVKAALKGALVPIVCIGETLEAREAGDTFKVLERQLKGGLEGLTGTQFA
PVIVAYEPVWAIGTGKVASDDQAQEAHAFIRGVIAGLFGKSAADKVRILYGGSVKPDNVKGLMSRPDIDGALVGGASLKG
ASFASIVRYSE
>Mature_251_residues
MRKPVIAGNWKLFKTKNEALALIEELAPLVSGVDSVEIVVAPVFTVLPTLPAALAGTGISLAAQDVFWEEEGAFTGEVSP
RMLLDAGASHVIIGHSERRQYFGETEETVNKKVKAALKGALVPIVCIGETLEAREAGDTFKVLERQLKGGLEGLTGTQFA
PVIVAYEPVWAIGTGKVASDDQAQEAHAFIRGVIAGLFGKSAADKVRILYGGSVKPDNVKGLMSRPDIDGALVGGASLKG
ASFASIVRYSE

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=247, Percent_Identity=44.1295546558704, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI226529917, Length=247, Percent_Identity=44.1295546558704, Blast_Score=177, Evalue=5e-45,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=44.6215139442231, Blast_Score=201, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17536593, Length=241, Percent_Identity=44.3983402489627, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=238, Percent_Identity=43.2773109243698, Blast_Score=177, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28572004, Length=245, Percent_Identity=45.3061224489796, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI28572008, Length=241, Percent_Identity=43.5684647302905, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI28572006, Length=241, Percent_Identity=43.5684647302905, Blast_Score=183, Evalue=8e-47,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_GEOBB (B5EF41)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139146.1
- GeneID:   6782334
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2338
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26431; Mature: 26431

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPVIAGNWKLFKTKNEALALIEELAPLVSGVDSVEIVVAPVFTVLPTLPAALAGTGIS
CCCCEEECCEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
LAAQDVFWEEEGAFTGEVSPRMLLDAGASHVIIGHSERRQYFGETEETVNKKVKAALKGA
HHHHHHEECCCCCEECCCCCHHEECCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHCC
LVPIVCIGETLEAREAGDTFKVLERQLKGGLEGLTGTQFAPVIVAYEPVWAIGTGKVASD
CEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEECCCCCCCC
DQAQEAHAFIRGVIAGLFGKSAADKVRILYGGSVKPDNVKGLMSRPDIDGALVGGASLKG
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCEEEECCCCCCC
ASFASIVRYSE
CHHHHHHCCCC
>Mature Secondary Structure
MRKPVIAGNWKLFKTKNEALALIEELAPLVSGVDSVEIVVAPVFTVLPTLPAALAGTGIS
CCCCEEECCEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
LAAQDVFWEEEGAFTGEVSPRMLLDAGASHVIIGHSERRQYFGETEETVNKKVKAALKGA
HHHHHHEECCCCCEECCCCCHHEECCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHCC
LVPIVCIGETLEAREAGDTFKVLERQLKGGLEGLTGTQFAPVIVAYEPVWAIGTGKVASD
CEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEECCCCCCCC
DQAQEAHAFIRGVIAGLFGKSAADKVRILYGGSVKPDNVKGLMSRPDIDGALVGGASLKG
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCEEEECCCCCCC
ASFASIVRYSE
CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA