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The map label for this gene is moaA-3 [H]

Identifier: 197118653

GI number: 197118653

Start: 2642891

End: 2643871

Strand: Reverse

Name: moaA-3 [H]

Synonym: Gbem_2272

Alternate gene names: 197118653

Gene position: 2643871-2642891 (Counterclockwise)

Preceding gene: 197118658

Following gene: 197118652

Centisome position: 57.29

GC content: 64.73

Gene sequence:

>981_bases
ATGGCACTGATAGATACCTACGGCAGGCGCATCAACTACCTTCGGCTCTCCGTCACCGACCGCTGCAACCTCCGTTGCCG
CTACTGCATGCCGGAAGAGGGGGTCGAGAAGCTGGACCACTCGCAGGTCCTCTCCTACGCCGATTTGTTGCGCATCTCGA
CCGAGGCGGTTGCCGCCGGCATCGAGAAGATCCGCGTCACCGGCGGGGAGCCCCTGGTCCGCAAGGGGATAATCTCCTTC
CTGGAGCGCCTGGGCGCGCTCCCCGGGCTCAAGGAGCTGGTACTGACCACGAACGGCCTGCTCCTTAAGGAGATGGCGCA
GGGATTGCGGGAAGCGGGGGTGCAGCGCTTGAACATCAGTCTCGATTCGCTGAAGCCCGAGACCTTCGCGGCAATCACCC
GCGGCGGAGAGCTTAAGCGCGTCTTGGACGGCCTTGAAGCCGCCGAGAAAGCTGGTTTCCCGCCGCACAAGATCAACGTG
GTGGTGATGCGCGGTGTAAACGACGACGAGATCCTCGACTTCGTGGAACTGACCATGAAGCGCCCCTACGCGGTCCGATT
CATCGAGTACATGCCCACCTGCGGCGACGCCGACTGGCGCGAACTCTGCGTGCCGGGGGCCGAAATCAGGGAGCGCATCG
GCGAGCGATACCTCATCGAGGAAGTGAGCAACAGCGAGCTCTCCGGCCCTTCGAAGAACTTCAAGGTGCAGGGGGCGCAG
GGGTCGCTCGGGATCATCACCGCCATGACCGGGCACTTCTGCAACGGCTGCAACCGGTTGCGGGTGACCGCCTCCGGGAT
CGCCAAGGGGTGCCTCTTCTCAGGCGACGGGGTGGACCTGCGGCCGGTTTTGGCCACAGGGGACGACGCCCTTTTGCGCC
AGGAGGTGCAGCGCATCGTGGCCGCGAAACCTGGCCGGCACGAGGTGACGGACGAGGGCGCGGAAACGGTCCCCTTCGCC
ATGTCCAGGGTTGGCGGATGA

Upstream 100 bases:

>100_bases
TGGTTTTTCGCATGCTGCCGGAACCCGACTGGGAGAGGGCGGCGACATTCGTATTCCAGCCGTCGGCTGCAGCCGGCGGC
TTCAGGGAAGAAGCTCAGCT

Downstream 100 bases:

>100_bases
TCAAGCGGCAGGTTGAGATTGTTTGGATATAGATTTGATTCAACAAGGAGTTAGCTATGACTGGAAAAATCATCGCGGTG
AACGTGAGCCTCAACAAGGG

Product: pyranopterin triphosphate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MALIDTYGRRINYLRLSVTDRCNLRCRYCMPEEGVEKLDHSQVLSYADLLRISTEAVAAGIEKIRVTGGEPLVRKGIISF
LERLGALPGLKELVLTTNGLLLKEMAQGLREAGVQRLNISLDSLKPETFAAITRGGELKRVLDGLEAAEKAGFPPHKINV
VVMRGVNDDEILDFVELTMKRPYAVRFIEYMPTCGDADWRELCVPGAEIRERIGERYLIEEVSNSELSGPSKNFKVQGAQ
GSLGIITAMTGHFCNGCNRLRVTASGIAKGCLFSGDGVDLRPVLATGDDALLRQEVQRIVAAKPGRHEVTDEGAETVPFA
MSRVGG

Sequences:

>Translated_326_residues
MALIDTYGRRINYLRLSVTDRCNLRCRYCMPEEGVEKLDHSQVLSYADLLRISTEAVAAGIEKIRVTGGEPLVRKGIISF
LERLGALPGLKELVLTTNGLLLKEMAQGLREAGVQRLNISLDSLKPETFAAITRGGELKRVLDGLEAAEKAGFPPHKINV
VVMRGVNDDEILDFVELTMKRPYAVRFIEYMPTCGDADWRELCVPGAEIRERIGERYLIEEVSNSELSGPSKNFKVQGAQ
GSLGIITAMTGHFCNGCNRLRVTASGIAKGCLFSGDGVDLRPVLATGDDALLRQEVQRIVAAKPGRHEVTDEGAETVPFA
MSRVGG
>Mature_325_residues
ALIDTYGRRINYLRLSVTDRCNLRCRYCMPEEGVEKLDHSQVLSYADLLRISTEAVAAGIEKIRVTGGEPLVRKGIISFL
ERLGALPGLKELVLTTNGLLLKEMAQGLREAGVQRLNISLDSLKPETFAAITRGGELKRVLDGLEAAEKAGFPPHKINVV
VMRGVNDDEILDFVELTMKRPYAVRFIEYMPTCGDADWRELCVPGAEIRERIGERYLIEEVSNSELSGPSKNFKVQGAQG
SLGIITAMTGHFCNGCNRLRVTASGIAKGCLFSGDGVDLRPVLATGDDALLRQEVQRIVAAKPGRHEVTDEGAETVPFAM
SRVGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=315, Percent_Identity=41.5873015873016, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI22027536, Length=315, Percent_Identity=41.5873015873016, Blast_Score=245, Evalue=4e-65,
Organism=Escherichia coli, GI1786999, Length=305, Percent_Identity=34.7540983606557, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI32566331, Length=319, Percent_Identity=40.4388714733542, Blast_Score=240, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI32566329, Length=327, Percent_Identity=39.7553516819572, Blast_Score=237, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17561210, Length=328, Percent_Identity=39.0243902439024, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI28574490, Length=316, Percent_Identity=41.1392405063291, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI28574488, Length=311, Percent_Identity=41.4790996784566, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 35646; Mature: 35514

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIDTYGRRINYLRLSVTDRCNLRCRYCMPEEGVEKLDHSQVLSYADLLRISTEAVAAG
CCCHHHHCCEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKIRVTGGEPLVRKGIISFLERLGALPGLKELVLTTNGLLLKEMAQGLREAGVQRLNIS
HHHEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEE
LDSLKPETFAAITRGGELKRVLDGLEAAEKAGFPPHKINVVVMRGVNDDEILDFVELTMK
HHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHC
RPYAVRFIEYMPTCGDADWRELCVPGAEIRERIGERYLIEEVSNSELSGPSKNFKVQGAQ
CCHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHCHHHHHHHHCCCCCCCCCCCEEEECCC
GSLGIITAMTGHFCNGCNRLRVTASGIAKGCLFSGDGVDLRPVLATGDDALLRQEVQRIV
CCCEEEEHHHHHHHCCCCEEEEEHHHHHHHCEECCCCCCEEEEEECCCHHHHHHHHHHHH
AAKPGRHEVTDEGAETVPFAMSRVGG
HCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
ALIDTYGRRINYLRLSVTDRCNLRCRYCMPEEGVEKLDHSQVLSYADLLRISTEAVAAG
CCHHHHCCEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKIRVTGGEPLVRKGIISFLERLGALPGLKELVLTTNGLLLKEMAQGLREAGVQRLNIS
HHHEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEE
LDSLKPETFAAITRGGELKRVLDGLEAAEKAGFPPHKINVVVMRGVNDDEILDFVELTMK
HHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHC
RPYAVRFIEYMPTCGDADWRELCVPGAEIRERIGERYLIEEVSNSELSGPSKNFKVQGAQ
CCHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHCHHHHHHHHCCCCCCCCCCCEEEECCC
GSLGIITAMTGHFCNGCNRLRVTASGIAKGCLFSGDGVDLRPVLATGDDALLRQEVQRIV
CCCEEEEHHHHHHHCCCCEEEEEHHHHHHHCEECCCCCCEEEEEECCCHHHHHHHHHHHH
AAKPGRHEVTDEGAETVPFAMSRVGG
HCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA