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The map label for this gene is ackA [H]

Identifier: 197118658

GI number: 197118658

Start: 2647823

End: 2649088

Strand: Reverse

Name: ackA [H]

Synonym: Gbem_2277

Alternate gene names: 197118658

Gene position: 2649088-2647823 (Counterclockwise)

Preceding gene: 197118660

Following gene: 197118653

Centisome position: 57.4

GC content: 61.06

Gene sequence:

>1266_bases
ATGGATATACTCGCACTTAACTGCGGAAGTTCTTCTGTAAAATACCAGTTGTTTGACTGGGACAAAAAAGTCGTGGTCGC
CAAGGGTATGGTGGAGCGCGTCGTCATCGGCGATTCGTTCATCCTGCACGAAGTCCCGGGCCGCGAGACCTACCGCGAGG
ATTCCGACTGTCCCGATCATAAGACCGCCGTCGATCTGATTCTGCGCACCCTGACCTCTCCCGGACACGGCGTGCTTACC
GATATCAAACAGATAGCCGCGGTTGGGCACCGCGTGGTACACGGGGGGGAGCGTTTCACCAAGTCCGTGCTCATCGATGA
TGACGTGCTGGCCGCGGTTACCGAGGTGCAGCATCTGGCGCCTTTGCACAACCCGCCCAATATCGCAGGCATCGAGGGGG
CCATGGCCGTTCTTCCTGGCGTCCCCCAGGTGGCAATTTTCGATACCGCGTTTCACCAGACCATGCCCGAACACGCCTAT
CTCTACCCGCTTCCCTACGAGTGGTACGAGAAGTACGGGGTCAGGCGGTACGGCTTCCACGGCACCTCGCATCTCTACGT
CTCCAAGCGCATCGCCGCCATCCTCGGCAAACCTGCGAACCAGTGCAACGTCATCACCATGCATATCGGTAACGGCGTAT
CGCACTGCGCCATCAAGAATGGGGTGTCCGTCGACACCAGCATGGGGCTCACCCCGCTGGAAGGCGCCATGATGGGTACC
CGCTGCGGCGACATCGACCCGGCCATCCCCGCCTTCATGATGCAGAAGGAAAACCTCTCCGCCAAGGAGATCGACAGCAT
CCTGAACAAGAAGAGCGGCGTCATCGGCATCACCGGCCGTTTCACCGACCGGCGTGACGTGATCGAGCACGCGAACGGGG
GCGACAACCTCTGCCGACTCGCGCTCGACATCGAGGCTTACCGTCTCAAGAAGTACATCGGCACCTACATGGCCGTGGTG
GGAAGGCTCGACGCGGTCGTCTTCACCGCCGGGGTGGGGGAGATGGGGGCTCCGATCAGGGAGCGCGCCATCGAGGGGCT
CGACCACCTGGGCATCGTCCTCGACCGCGAGCGCAACGCCTCCGCCATGACCAGGAAGCGCGAGACGCTGATCACCACCG
ACGACTCGCCGGTGAAGGTGTACGTGATACCGACAGACGAGGAGCTGGTCTTCACTGAAGACGTGGCGGCGATCCTGGAC
GGGACCTACACGGACCACATGCACTTCGAGTACAGTTTCTCCAGGCCCGACTTCGTCAGGAAGTAA

Upstream 100 bases:

>100_bases
TACGGTATATTTAAAAAACAGTTTTATTTGTAAACTTTTTTTGCCGGACTAAAGTACCCAGCTTGCCCGGTTAAACTACA
ATGACGGGGGATGCAGATCG

Downstream 100 bases:

>100_bases
GGCGCGGCAGAGCGTAAGGCAACAAAAAGGCCGGCGGGGTAACCCGCCGGCCTTTCTTTTTGTTCTTTGTGTAAGCGAGC
CGCCGCCAGTGGTTGACAGG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MDILALNCGSSSVKYQLFDWDKKVVVAKGMVERVVIGDSFILHEVPGRETYREDSDCPDHKTAVDLILRTLTSPGHGVLT
DIKQIAAVGHRVVHGGERFTKSVLIDDDVLAAVTEVQHLAPLHNPPNIAGIEGAMAVLPGVPQVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLYVSKRIAAILGKPANQCNVITMHIGNGVSHCAIKNGVSVDTSMGLTPLEGAMMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVIGITGRFTDRRDVIEHANGGDNLCRLALDIEAYRLKKYIGTYMAVV
GRLDAVVFTAGVGEMGAPIRERAIEGLDHLGIVLDRERNASAMTRKRETLITTDDSPVKVYVIPTDEELVFTEDVAAILD
GTYTDHMHFEYSFSRPDFVRK

Sequences:

>Translated_421_residues
MDILALNCGSSSVKYQLFDWDKKVVVAKGMVERVVIGDSFILHEVPGRETYREDSDCPDHKTAVDLILRTLTSPGHGVLT
DIKQIAAVGHRVVHGGERFTKSVLIDDDVLAAVTEVQHLAPLHNPPNIAGIEGAMAVLPGVPQVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLYVSKRIAAILGKPANQCNVITMHIGNGVSHCAIKNGVSVDTSMGLTPLEGAMMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVIGITGRFTDRRDVIEHANGGDNLCRLALDIEAYRLKKYIGTYMAVV
GRLDAVVFTAGVGEMGAPIRERAIEGLDHLGIVLDRERNASAMTRKRETLITTDDSPVKVYVIPTDEELVFTEDVAAILD
GTYTDHMHFEYSFSRPDFVRK
>Mature_421_residues
MDILALNCGSSSVKYQLFDWDKKVVVAKGMVERVVIGDSFILHEVPGRETYREDSDCPDHKTAVDLILRTLTSPGHGVLT
DIKQIAAVGHRVVHGGERFTKSVLIDDDVLAAVTEVQHLAPLHNPPNIAGIEGAMAVLPGVPQVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLYVSKRIAAILGKPANQCNVITMHIGNGVSHCAIKNGVSVDTSMGLTPLEGAMMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVIGITGRFTDRRDVIEHANGGDNLCRLALDIEAYRLKKYIGTYMAVV
GRLDAVVFTAGVGEMGAPIRERAIEGLDHLGIVLDRERNASAMTRKRETLITTDDSPVKVYVIPTDEELVFTEDVAAILD
GTYTDHMHFEYSFSRPDFVRK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=43.3249370277078, Blast_Score=314, Evalue=5e-87,
Organism=Escherichia coli, GI145693184, Length=395, Percent_Identity=39.2405063291139, Blast_Score=292, Evalue=2e-80,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 46364; Mature: 46364

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILALNCGSSSVKYQLFDWDKKVVVAKGMVERVVIGDSFILHEVPGRETYREDSDCPDH
CCEEEEECCCCCEEEEEECCCCEEEEECCHHHEEEECCCEEEECCCCCCCCCCCCCCCCH
KTAVDLILRTLTSPGHGVLTDIKQIAAVGHRVVHGGERFTKSVLIDDDVLAAVTEVQHLA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHHHHHHH
PLHNPPNIAGIEGAMAVLPGVPQVAIFDTAFHQTMPEHAYLYPLPYEWYEKYGVRRYGFH
CCCCCCCCCCCCCHHHHCCCCCCEEEEHHHHHHCCCCCEEEECCCHHHHHHCCCEEECCC
GTSHLYVSKRIAAILGKPANQCNVITMHIGNGVSHCAIKNGVSVDTSMGLTPLEGAMMGT
CCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCC
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVIGITGRFTDRRDVIEHANGGDNLCRL
CCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCEEEE
ALDIEAYRLKKYIGTYMAVVGRLDAVVFTAGVGEMGAPIRERAIEGLDHLGIVLDRERNA
EEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCHHHCCEEEECCCCC
SAMTRKRETLITTDDSPVKVYVIPTDEELVFTEDVAAILDGTYTDHMHFEYSFSRPDFVR
HHHHHCCCEEEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCEEEEEECCCCCCCC
K
C
>Mature Secondary Structure
MDILALNCGSSSVKYQLFDWDKKVVVAKGMVERVVIGDSFILHEVPGRETYREDSDCPDH
CCEEEEECCCCCEEEEEECCCCEEEEECCHHHEEEECCCEEEECCCCCCCCCCCCCCCCH
KTAVDLILRTLTSPGHGVLTDIKQIAAVGHRVVHGGERFTKSVLIDDDVLAAVTEVQHLA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHHHHHHH
PLHNPPNIAGIEGAMAVLPGVPQVAIFDTAFHQTMPEHAYLYPLPYEWYEKYGVRRYGFH
CCCCCCCCCCCCCHHHHCCCCCCEEEEHHHHHHCCCCCEEEECCCHHHHHHCCCEEECCC
GTSHLYVSKRIAAILGKPANQCNVITMHIGNGVSHCAIKNGVSVDTSMGLTPLEGAMMGT
CCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCC
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVIGITGRFTDRRDVIEHANGGDNLCRL
CCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCEEEE
ALDIEAYRLKKYIGTYMAVVGRLDAVVFTAGVGEMGAPIRERAIEGLDHLGIVLDRERNA
EEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCHHHCCEEEECCCCC
SAMTRKRETLITTDDSPVKVYVIPTDEELVFTEDVAAILDGTYTDHMHFEYSFSRPDFVR
HHHHHCCCEEEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCEEEEEECCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA